A Critical Role of Fatty Acid Binding Protein 4 and 5 (FABP4/5) in the Systemic Response to Fasting

学位記番号：医博甲148

Moringa oleifera Lam. to accelerate wound healing: a review

An injury to the skin that disrupts the soft tissue may form a wound. The healing process in response to injury is a dynamic and well-regulated process of cellular, humoral, and molecular mechanisms that consists of four partly overlapping phases: hemostasis, inflammation, proliferation, and remodeling. An impaired wound-healing process may cause a formation of an abnormal scar and chronic wounds, leading to a reduced life quality. Therefore, it needs an optimal prevention strategy. Many modalities have been claimed to accelerate wound healing. The trend of using natural products is increasing in most Southeast Asian countries due to their biodiversity. Nowadays, studies on natural compounds are increasing to accelerate wound healing. Moringa oleifera Lam. is a high-value plant that each part of it has a high nutritional value as well as a great range of medicinal uses, including anti-inflammatory, antimicrobial, antioxidant, and wound healing properties. In this review, we have explored the M. oleifera that are very rich in vitamins, minerals, fatty acids, and phytochemical compounds like quercetin, kaempferol, and vicenin-2, that play a role in the wound healing process. Moreover, these compounds may enhance the healing of wounds with pathological conditions such as diabetes, immunocompromised and persistent infection

Viabilitas Oosit Pasca Vitrifikasi menggunakan Kombinasi Ethilen Glikol dan Dimetil Sulfoksida dengan Dua Level Konsentrasi yang Berbeda

Vitrifikasi merupakan metode kriopreservasi untuk membekukan sel secara cepat, tanpa disertai terbentuknya kristal es. Vitrifikasi dilakukan dengan menggunakan krioprotektan yang memiliki toksitas. rendah sehingga oosit dapat mempertahankan viabilitasnya. Dimetil sulfoksida (DMSO) dan ethylene glycol (EG) merupakan krioprotektan intraseluler dengan toksisitas rendah sehingga kombinasi kedua krioprotektan tersebut diharapkan dapat meningkatkan viabilitas oosit pasca vitrifikasi. Tujuan dari penelitian ini adalah untuk mengevaluasi viabilitas oosit pasca vitrifikasi dengan menggunakan kombinasi dan DMSO dan EG pada konsentrasi yang berbeda. Penelitian dilaksanakan di Laboratorium Riset dan Bioteknologi, Fakultas Peternakan, Universitas Padjadjaran periode September 2016-Desember 2016. Penelitian menggunakan Rancangan Acak Lengkap dengan dua kelompok perlakuan,  yaitu media vitrifikasi dengan dua konsentrasi yang berbeda: 15% DMSO+15% EG dan media 17% DMSO+17%EG. Setelah seminggu penyimpanan, maka dilakukan proses warming untuk mengevaluasi viabiltias oosit pasca vitrifikasi. Hasil menunjukkan bahwa viabilitas oosit yang divitrifikasi dengan menggunakan 17% DMSO+17%EG nyata lebih tinggi apabila dibandingkan dengan 15% DMSO+15%EG. Kata kunci: vitrifikasi, Dimetil sulfoksida, Ethylen glikol, viabilitas oosit

Perbandingan Viabilitas Oosit Domba Pasca Vitrifikasi dengan Menggunakan Hemistraw dan Cryotop

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengkaji efek vitrifikasi dengan menggunakan dua buah system carrier yang berbeda terhadap viabilitas oosit domba yang telah dimaturasi secara in vitro. Oo­sit dibagi menjadi dua kelompok perlakuan, yaitu (i) divitrifikasi dengan menggunakan hemistraw (ii) divitrifikasi dengan menggunakan cryotop. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vi­trifikasi serupa pada kedua jenis carrier yang digu­nakan untuk vitrifikasi. Oosit diletakkan dalam larutan equilibrasi yang mengandung konsentrasi permeable kriopro­tektan setengah dari larutan vitrifikasi. Se­telah 15 menit, oosit ditransfer ke dalam media vitrifikasi yang mengandung 17% EG+17% DMSO +0, 65M sukrosa di dalam modified PBS yang dis­uplementasi dengan 20% fetal bovine serum. Total waktu yang digunakan untuk memaparkan oosit ke dalam laru­tan vitrifikasi adalah 30 detik. 5-8 oosit dipipet menggunakan kapiler gelas dan diletak­kan/loading ke dalam carrier yang digunakan (hemistraw atau cryotop) kemudian langsung di paparkan ke dalam nitrogen cair. Viabilitas oosit dievaluasi berdasarkan reekspansi, warna dan homogenitas sitoplasma. Hasil penelitian ini menunjukkan bahwa viabilitas oosit setelah vi­trifikasi serupa pada kedua jenis carrier yang digu­nakan untuk vitrifikasi(Comparation of sheep oocyte viability after vitrification using hemistraw and cryotop)ABSTRACT. The aim of this study was to examine the effect vitrification using two different carrier system on the matured sheep oocytes viability. Oocytes were devided into two group (i) vitrified using hemistraw (ii) vitrified using cryotop. Oocytes placed into equilibration solution which is containing a half concentration permeable cryoprotectant of vitrification solution. After 15 minute, oocytes were transferred into vitri­fication solution containing 17% EG+17% DMSO +0, 65M sucrose in modified PBS supplemented with 20% fetal bovine serum. The total exposure time of oocytes to vitrification solution was 30 sec. Oocytes were pipetted into a glass capillary into group 5-8, and loaded into carrier (hemistraw or cryotop) then plugged into liquid nitrogen. After a week cryopreservation, oocytes were warmed and cultured in TCM 199 suplemented with 10% fetal bovine serum at 38.50 C under 5% CO2 for 3h. Oo­cytes viability was evaluated by re expansion, color and homogeneity of oocyte cytoplasm. Our results indicated that the oocytes viability after vitrification was similar from both of carrier for vitrificatio

THE HEMATOLOGIC PROFILE IN THE ACUTE TOXICITY TEST OF COGON GRASS ROOTS ETHANOL EXTRACT IN WISTAR RATS

Objective: This study aimed to investigate the hematologic profile of Wistar rats in the acute toxicity test of Cogon grass roots ethanol extract (CGEE). Methods: Cogon grass roots were dissolved in 70% ethanol. An acute toxicity test was conducted based on The National Agency of Drug and Food Control of the Republic of Indonesia. Five female rats in the treatment group were administered a single high dose of 5000 mg/kg body weight (BW) of CGEE in 200 μl of 0.5% carboxymethyl cellulose (CMC), and the 5 female rats in the control group were administered 200 μl of 0.5% CMC. After 14 d, blood samples were collected, and 18 hematologic parameters were measured with a hematology analyzer. Statistical analyses were performed to compare the parameters between the two groups with the independent t-test for normally distributed data and the Mann Whitney test for non-normally distributed data. Results: None of the hematologic parameters in the treatment group significantly differed from those in the control group after 14 d of observation (P>0.05). Conclusion: A single high dose of 5000 mg/kg BW of CGEE did not change the hematologic profile of Wistar rats. These results indicate that CGEE does not have an acute hemotoxic effect, at least for hematologic parameters

Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method

Objective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxygen species (ROS) that reduces sperm quality.Methods: Semen samples that were collected from 31 male partners of couples undergoing infertility investigation were analyzed for sperm concentration, motility, and leucocytes concentration. Semen samples were then divided in two groups based on their leucocytes concentrations (category A: >0 to 1 x 106/mL. Semen samples were processed using density-gradient centrifugation technique. Results: There was a significant difference in the number of sperms harvested and sperm motility after preparation. Interestingly, pregnancy rate after IUI was higher (p<0.05) in non-leukocytospermia semen (39%) when compared to leukocytospermia semen (30%).Conclusions: Seminal leukocytes (PMNL) concentration affects pregnancy rate after intrauterine insemination. Keywords: Density gradient method, sperm recovery rate, intrauterine insemination, pregnancy rate DOI: 10.15850/ijihs.v6n2.131

THE EFFECT OF COGON GRASS ROOT ETHANOL EXTRACT ON REPRODUCTIVE ORGAN AND EPIDIDYMAL SPERM QUALITY OF MOUSE WITH DIFFERENT AGES

This study aims to determine changes related to epididymal sperm parameters in mice given cogon grass root ethanol extract (CGREE). The experiment was carried out by dividing three groups of mice aged 4-week old (early pubertal), 8-week old (adult), and 24-week old (old) as control and treatment groups. The treatment groups were given 115 mg/kg body weight of CGREE for 14 days. The mice were sacrificed on day 15 to asses reproductive organ indexes and quality of the epididymal sperm. Index of epididymis and ductus deferens indices significantly reduced in the 4-week old treatment group compared to control (0.23 vs 0.33; 0.08 vs 0.16,  respectively), and vesical seminal significantly reduced in 8- week old treatment group compared to control (0.76  vs  0.68). The epididymal sperm quality significantly decreased in all treatment groups, but the sperm concentration in early pubertal group showed an increase. These results indicate that CGREE can enhance several semen parameters in prepubertal and adult mice but this effect is not apparent in old mice

Pengaruh Keberadaan Corpus Luteum terhadap Kualitas dan Tingkat Maturasi Oosit Domba Lokal Umur Pubertas Awal Secara In Vitro

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 (>4 lapis), grade 2 (3–4 lapis) dan grade 3 (0–2 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5°C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p<0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro.   (Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty)  ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by >4, 3–4, and 0–2 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5°C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p<0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production progra

PRELIMINARY PRELIMINARY STUDY ON BLOOD PROFILE OF BLOOD PRESSURE AND VEGETABLE CONSUMPTION AMONG WOMEN IN THE ERA OF GERMAS IN CICANIR AND JATIPAMOR VILLAGES, MAJALENGKA REGENCY, WEST JAVA PROVINCE

Objective: To discover the profile of hypertension (HT) disease and consumption habit of vegetables among women in Cicanir and Jatipamor villages related to the program of Gerakan Masyarakat Hidup Sehat (GERMAS)/Healthy Living Society Movement. Method: The study used a cross-sectional design while respondents were taken by using a simple random sampling. The blood pressure profile was classified based on the Seventh Report of the Joint National Committee (JNC 7). The respondents suffering from HT were given a question relating to their blood pressure. Vegetable consumption profile of the respondents was measured by using four questions. Result: Youngest ages of pre-HT respondents in Cicanir and Jatipamor were 16 and 18 y, respectively; youngest ages of HT respondents in Cicanir and Jatipamor were 22 and 18 y, respectively. Numbers of the respondents with HT in Cicanir and Jatipamor villages had increased into 16.3% and 10.9%, respectively. Vegetables consumption frequencies for 4-7 d/w in Cicanir and Jatipamor were 81.33% and 62.66%, respectively. The respondents cleaning vegetables under running water in Cicanir and Jatipamor were 74.67% and 86.67%, respectively. The respondents who cooked the vegetables optimally in Cicanir and Jatipamor were 46.67% and 93.33%, respectively. Based on vegetable sources, the respondents bought the vegetables in the markets in Cicanir and Jatipamor were 81.33% and 93.33%, respectively. Conclusion: In both villages, HT prevalence had increased. Vegetable consumption profile found in the respondents showed the respondents had a good vegetable consumption habit. It is important to improve awareness of respondents to maintain health for themselves and their families