Personal private herbaria: a valuable but neglected source of floristic data. The case of Italian collections today

Today private collections still represent an important source of information as is evident from the available literature, where, in many cases, private collections are mentioned both in floristic and taxonomic/systematic researches. We present a summary of the main information about private herbaria in Italy, whose collections are currently increasing. Based on our survey, we retrieved information on 34 personal herbaria where 156,361 specimens are preserved at present. Piedmont and Tuscany resulted the two Italian regions with the highest number of collections, whereas for 9 regions we obtained no answer. The most represented families resulted Asteraceae and Poaceae, whereas the most represented genera resulted Carex, Trifolium, and Hieracium. Taken all together, these collections rank 16th among the 68 institutional public herbaria officially recognised in Italy

Vascular flora of Monte Sparviere (southern Italy, Pollino Massif)

Vascular Flora of Monte Sparviere (Southern Italy, Pollino Massif). A floristic survey of Monte Sparviere was carried out from 2012 to 2015, allowing us to record 377 specific and subspecific taxa, belonging to 229 genera and 64 families. The most represented families are Asteraceae (55 taxa), Poaceae (30), Fabaceae (28), Rosaceae (23) and Lamiaceae (19). Italian endemic species reach the 8.5% and no exotic species are recorded except three conifers used for reforestation. Biological spectrum shows a dominance of Hemicryptophytes, with a moderate percentage of Therophytes. The chorological analysis shows a dominance of species belonging to the Eurosibiric region, albeit Mediterranean region is also well represented. The ecological spectra are in agreement with climatic and geo-pedologic features, with variations mainly related to woody coverage and altitude. Finally, Potentilla pedata Willd. ex Hornem was confirmed for the flora of Basilicata; Dianthus sternbergii Capelli was excluded from the flora of Basilicata and Calabria whereas Dianthus hyssopifolius L. resulted new for both regions

The Stability of C6H6+2: The Implication for Polycyclic Aromatic Hydrocarbon Dications

The fragmentation pathways of C6H are studied using density functional theory. All of the transition states that were found, excluding the rearrangement to the C5v structure, are significantly above the six-membered ring structure. This is consistent with the soft ionization experiments that find mostly dication production instead of fragmentation. Since the C5v structure is probably of limited importance for multiple-ring polycyclic aromatic hydrocarbons, the computed barriers suggest that Coulomb explosion is not a significant channel under most astrophysical conditions, and therefore, the dications are long-lived and should be considered in modeling of the interstellar medium

A morphometric and karyological study of Onobrychis calabrica (Fabaceae), a neglected species endemic to Calabria, S Italy

A morphometric (multivariate and univariate) and karyological study of Onobrychis calabrica Sirj. (Fabaceae), compared to O. alba subsp. echinata (Guss.) P. W. Ball and O. supina (Chaix ex Vill.) DC., was carried out. According to our results, twelve morphological characters clearly support the distinctiveness of O. calabrica. In addition, the latter species is tetraploid with 2n = 4x = 28 chromosomes, whereas the other two taxa are diploid with 2n = 2x = 14 chromosomes. The names O. calabrica and O. handel-mazzettii Sirj. (a heterotypic synonym of O. supina) are lectotypified here. Onobrychis calabrica, a narrow endemic to SE Calabria, S Italy, is here assessed as Critically Endangered CR B1ab(iii)+B2ab(iii) following IUCN Red List categories and criteria

A simple and quick method for loading proteins in extracellular vesicles

Extracellular vesicles (EVs) mediate intercellular transport of biomolecular cargo in the body, making them promising delivery vehicles for bioactive compounds. Genetic engineering of producer cells has enabled encapsulation of therapeutic proteins in EVs. However, genetic engineering approaches can be expensive, time-consuming, and incompatible with certain EV sources, such as human plasma and bovine milk. The goal of this study was to develop a quick, versatile, and simple method for loading proteins in EVs post-isolation. Proteins, including CRISPR associated protein 9 (Cas9), were bound to cationic lipids that were further complexed with MDA-MB-231 cell-derived EVs through passive incubation. Size-exclusion chromatography was used to remove components that were not complexed with EVs. The ability of EVs to mediate intracellular delivery of proteins was compared to conventional methods, such as electroporation and commercial protein transfection reagents. The results indicate that EVs retain native features following protein-loading and obtain similar levels of intracellular protein delivery as conventional methods, but display less toxicity. This method opens up opportunities for rapid exploration of EVs for protein delivery

Chromosome numbers for the Italian flora: 2.

In this contribution new chromosome numbers for Italian endemic taxa are presented. It includes 13 chromosome counts for Ornithogalum (Asparagaceae), Anthemis, Carduus, Centaurea, Cirsium, Hieracium, Taraxacum (Asteraceae), Asyneuma (Campanulaceae), Knautia (Caprifoliaceae), Gypsophila (Caryophyllaceae), Linum (Linaceae), Helleborus (Ranunculaceae)

Chromosome numbers for the Italian flora: 2.

In this contribution new chromosome numbers for Italian endemic taxa are presented. It includes 13 chromosome counts for Ornithogalum (Asparagaceae), Anthemis, Carduus, Centaurea, Cirsium, Hieracium, Taraxacum (Asteraceae), Asyneuma (Campanulaceae), Knautia (Caprifoliaceae), Gypsophila (Caryophyllaceae), Linum (Linaceae), Helleborus (Ranunculaceae)

Chromosome numbers for the Italian flora: 1.

In this contribution new chromosome data obtained on material collected in Italy are presented. It includes 15 chromosome counts for Carduus, Crepis, Picris, Taraxacum (Asteraceae), Ceratonia, Lathyrus (Fabaceae), Colchicum (Colchicaceae), Fritillaria (Liliaceae), Petrorhagia (Caryophyllaceae), Potentilla (Rosaceae), Quercus (Fagaceae), Reseda (Resedaceae), and Thymus (Lamiaceae)

Chromosome numbers for the Italian flora: 3

In this contribution new chromosome data obtained from material collected in Italy are presented. It includes four chromosome counts for the following genera: Bellevalia (Asparagaceae), Genista (Fabaceae), Linaria (Plantaginaceae), and Teucrium (Lamiaceae)