Green synthesis of nano binary oxide SiO2/V2O5 NPs integrated ointment cream application on wound dressings and skin cancer cells

 اشتملت الدراسة الحالية على تحضير المزدوج النانو SiO2/V2O5  باستخدام مواد طبيعية خام كمادة أولية حيث استخدمت رماد قشور الارز بعد حرقها بدرجة  °C700  سيليزية  للتخلص من المواد العضوية واللاعضوية في القشور حيث كانت هي  المادة الخام لاستخلاص السليكا جل والتي تم مزجها مع أوكسيد الفناديوم الخماسي وتم تشخيص المزدوج النانو SiO2/V2O5  المحضر باستخدام عدة تقنيات مثل  الأشعة السينية ,المجهر الالكتروني الماسح عالي الدقة حيث تم حساب معدل الحجم الحبيبي باستخدام صورة المجهر الالكتروني الماسح  للسطح المادة المحضرة عند المقياس 200 نانومتر , مطيافية تشتت الطاقة بالأشعة السينية التي استخدمت لكشف تركيز العناصر  الموجودة في النموذج المحضر SiO2/V2O5 من مادة قشور الارز الخام وتقديرها كميا ونوعيا  وكذلك تم قياس المساحة السطحية للسليكا المستخلص والمزدوج المحضر SiO2/V2O5 والمقارنة بينهما كما موضح في النتائج  كما تضمنت الدراسة البحثية الفعالية البيولوجية للمزدوج SiO2/V2O5 وتأثيرها على تثبيط النمو البكتيري بعد أن تم تطبيق المادة النانوية المحضرة على ضمادات الجروح حيث أعطت نتيجة واعدة لاستخدامها كضمادات موضعية تعمل على إزالة الميكروبات وخاصة لمرضى الحروق والجروح وذلك بسبب فعاليتها العالية لقتل البكتيريا الموجبة  S.aurea عند تركيزµg/mL  625   التي تتميز بمقاومتها للعديد من المضادات الحيوية . تعتبر مقاومة المضادات الحيوية من المشكلات التي يسعى العديد من الباحثين لحل هذه المشكلة من خلال توفير مضادات حيوية أكثر فعالية وأمانًا. اختيار مادة السيليكا المستخلصة من مادة طبيعية لتقليل السمية الناتجة عن استخدام الكيماويات ، حيث تعتبرالسيليكا مادة غير سامة. لذلك ، أثناء التحضير ، تم الحرص على استخدام المواد الكيميائية بتراكيز منخفضة لتقليل السمية. تم دراسة التأثيرات السامة للخلايا في المختبر SiO2 / V2O5)) NPs على خط الخلايا الطبيعية Vero 101 وخط خلايا الجلد A431 وتم فحصهما بتراكيز مختلفة. تم استخدام MTT (3- (4،5-dimethylthiazol-2-yl) -2،5-diphenyltetrazolium bromide) لتحديد التأثيرات السامة للخلايا للمساحيق النانوية الخضراء المُصنّعة.Green synthesis is depending on preparation of nano composited SiO2/V2O5 by using the modified sol-gel method depending on rice husk ash as a source for the extraction of silica gel and the product powder of nano composited SiO2/V2O5 characterization by many techniques such as X-ray diffraction spectroscopy (XRD), field emission scanning electron microscopy (FESEM), and N2 adsorptions/desorption isotherms (BET). This study also includs the biological effectiveness of SiO2/V2O5 and its effect on inhibiting bacterial growth after the prepared nanomaterial was applied to wound dressings, which gave a promising result for its use as topical dressings that remove microbes especially for burns and wounds patients, due to its high effectiveness in killing Gram-positive bacteria S.aurea positive bacteria at a concentration of 625 µg/mL, which is characterized by its resistance to many antibiotics. Antibiotic resistance is one of the problems that many researchers seek to solve this problem by providing more effective and safe antibiotics. Choosing silica extracted from a natural substance to reduce the toxicity resulting from the use of chemicals, as silica oxide is considered a non-toxic substance. Therefore, during preparation, care was taken to use chemicals in low concentrations to reduce toxicity. In vitro cytotoxic effects were studied of composited SiO2/V2O5 nanoparticles  on Vero cell line 101 and skin cell line-A431were investigated at different concentrations. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxic effects of green synthesized nanopowders

Investigation in Optical Properties of Magnesium Doped Nanostructure Zinc Oxide Films by Pulsed-Laser Deposition Method

In the present work, films have been grown under various deposition conditions in order to understand the effect of processing on the film properties and to specify the optimum condition, namely substrate at temperatures of 400°C, oxygen pressure (2×10-1) mbar, laser fluence 0.4 J/cm2, and with different Mg contents (x=0, 0.02, 0.04, 0.06), using double frequency Q-switching Nd:YAG laser beam (wavelength 532nm), repetition rate (1-6) Hz and the pulse duration of (10 ns), to deposit MgxZn1-xO films glass substrates with thickness of about 200 nm for all MgxZn1-xO films at different deposition condition and the number of laser pulses was 100 pulses. The optical properties were characterized by the transmittance and absorption spectroscopy at room temperature, measured in the range from 300 to 900 nm. For all the films, the average transmittance in the visible wavelength region λ = (400 - 800) nm is greater than 70%. The maximum value of the transmittance (greater than 95%) was obtained for these films. Eg values of MgxZn1-xO thin films were (3.37, 3.59, 3.82, and 4.00) eV corresponding to the Mg-content (x = 0, 0.02, 0.04 and 0.06), respectively. In other word, the optical band gap of MgxZn1-xO thin films become wider as Mg-content increases and can be precisely controlled between 3.37 and 4.00 eV. The refractive index of the films ranged from 2.1-2.8 between 350nm to 900nm. The extinction coefficient and the optical conductivity of the films increases with doped. The real dielectric constant and the imaginary part increases when the doped rate increasing. Keywords: Optical properties, Pulsed-laser deposition, MgxZn1-xO Nanostructures

FINITE ELEMENT ANALYSIS OF A SINGLE-PHASE INDUCTION MOTOR WITH NON-UNIFORM STATOR SLOTS BASED ON MAGNET SOFTWARE AND AUTOCAD

The current study uses the finite element technique to investigate a single-phase induction motor with non-uniform stator slots. A 0.5 hp, four poles, 36 stator slots, and 48 rotor slots have non-uniform stator slots were analyzed based on magnet Finite Element Method software with using AutoCAD in modeling stator due to its unsymmetrical slots, The suggested study attempts to evaluate all motor design modifications. The values of currents and torque received from the FEM analysis for the test motor were compared with motor nameplate data with a good agreement

Classification of COVID-19 from CT chest images using Convolutional Wavelet Neural Network

Analyzing X-rays and computed tomography-scan (CT scan) images using a convolutional neural network (CNN) method is a very interesting subject, especially after coronavirus disease 2019 (COVID-19) pandemic. In this paper, a study is made on 423 patients’ CT scan images from Al-Kadhimiya (Madenat Al Emammain Al Kadhmain) hospital in Baghdad, Iraq, to diagnose if they have COVID or not using CNN. The total data being tested has 15000 CT-scan images chosen in a specific way to give a correct diagnosis. The activation function used in this research is the wavelet function, which differs from CNN activation functions. The convolutional wavelet neural network (CWNN) model proposed in this paper is compared with regular convolutional neural network that uses other activation functions (exponential linear unit (ELU), rectified linear unit (ReLU), Swish, Leaky ReLU, Sigmoid), and the result is that utilizing CWNN gave better results for all performance metrics (accuracy, sensitivity, specificity, precision, and F1-score). The results obtained show that the prediction accuracies of CWNN were 99.97%, 99.9%, 99.97%, and 99.04% when using wavelet filters (rational function with quadratic poles (RASP1), (RASP2), and polynomials windowed (POLYWOG1), superposed logistic function (SLOG1)) as activation function, respectively. Using this algorithm can reduce the time required for the radiologist to detect whether a patient has COVID or not with very high accuracy

Tooth wear in relation to physical salivary characteris-tics among gastroesophageal reflux disease

Background: Gastroesophageal reflux disease, is a quite prevalent gastrointestinal disease, among which gastric content (excluding the air) returns into the oral cavity. Many 0ral manifestations related t0 this disease include tooth wear, dental caries also changes in salivary flow rate and pH. This study was conducted among gastroesophageal reflux disease patients in order to assess tooth wear in relation to salivary flow rate and pH among these patients and the effect of gastroesophageal reflux disease duration on this relation. Materials and methods: One hundred patients participate in this cross-sectional study for both genders and having an age range of 20-40 years old, patients had been endoscopically identified as having gastroesophageal reflux disease using the classification of Los Angeles (LA), who were attending the Gastroenterology and Hepatology Teaching Hospital in Baghdad. and divided into two groups: group A with grade severity mucosal breakage not longer than 5 mm and Group B with grade severity mucosal breakage more than 5 mm long, Smith and Knight (1984) tooth wear index criteria were used for the assessment of tooth wear. For measurement of salivary flow rate and pH, saliva sample (unstimulated) had been collected. Results: Of the entire sample (90%) were having tooth wear. Tooth wear was higher in grade B severity than in grade A severity among patients with gastroesophageal reflux disease duration of two years or less, while it was higher in grade A than in grade B among patients with a duration of more than two years but all these results were statistically non-significant. Salivary flow rate and pH showed a non-significant reduction with increasing gastroesophageal reflux disease severity for both of groups concerning disease duration. The correlation of total tooth wear with salivary flow rate and pH was a significantly weak negative correlation in grade A, while a nonsignificant weak negative correlation in grade B. Conclusions: The findings of the present study concluded that patients with gastroesophageal reflux disease recorded a high occurrence of tooth wear and there was a negative correlation of tooth wear with salivary flow rate and pH among patients with gastroesophageal reflux disease

L-Tryptophan and Thiamine Hydrochloride as Vital Factors of the Indole Acetic Acid and Siderophores Produced by Rhizobium leguminosarum bv. viceae and Their reflection on Faba Bean growth Yield and some Soil Properties under Saline Soil

Plant growth promoting rhizobacteria such as R. leguminosarum bv. viceae strains no. 481 & 441 were shown to produce indole-3-acetic acid (IAA) and siderophores in bacterial culture supplemented with L-tryptophan (L-trp) and thiamine hydrochloride (Thch) concentrations. Maximum IAA production was found in the medium amended with 1.5mg ml-1 (L-trp). IAA production was not observed with the (L-trp) in free medium while (Thch) produced a maximum amount of growth and IAA at 3 mg ml-1 for both the two rhizobial strains. Biosynthesis of siderophore was maximum (44.23% and 35.75%) at 1.5mg ml-1 of (L-trp ) while the production of sidrophore was (40.49% & 34.68 % of siderophore units) at 3 mg ml-1 of (Thch) in R. leguminosarum bv. viceae strain 481 & 441 respectively. Rhizobial strain 481 was more active in the production IAA and siderophore than the other rhizobial strain 441. A field experiment on a newly reclaimed saline soil at Sahl El-Hussinia, El-Sharkia Governorate, Egypt, used to evaluate the effect of IAA and siderophores synthesizing by rhizobial strains on plant growth parameters and soil characteristics. The obtained results revealed that applying of rhizobial strains grown on 1.5mg ml-1 L-Trp and 3 mg ml-1 (Thch) led to improve soil properties which reflected on the crop growth and yield components. Soil respiration (CO2 evolution) and proline content were also studied

Effect of Female Sex Hormones on Cardiorespiratory System: Theoretical Review

This study aimed at analyzing the effect of the female sex hormones upon  the cardiorespiratory system. And the results showed that the cardiac output measurement showed no significant increase during both phases of menstrual cycle before exercise but there was an insignificant increase soon after exercise owing to sympathetic over activity. The observed increase in the peripheral blood flow during mid-follicular phase before and after exercise revealed that influence of estrogen increases the arterial distensibility. The observed increase in the respiratory efficiency test during early luteal phase before and soon after exercise revealed the influence of progesterone, which is considered to be a potent stimulator of respiration, and added up effect of hormones and exercise-induced changes

SAPONINS, GLYCOSIDES AND FLAVONOIDS IN CELLS AND TISSUES OF BALANITES AEGYPTIACA CULTURED ON SOLID AND LIQUID CULTURE MEDIA

Objectives: The main objective of the present study is to obtain callus and cell suspension culture from Balanitesa egyptiaca sterile plantlets grown in vitro and to compare growth and the biosynthetic potential of saponins, flavonoids and glycosides by callus and cell suspension culture Balanitisa egyptiaca. Methods: Callus was induced from the mother plants on MS culture media supplemented with  2.0 mg/l  BA + 2.0 mg/l  2,4-D with and without agar gelling. Total saponins, glycosides and flavonoids were estimated in both types of cultures over a period extending from 1 to 5 weeks to compare the productivity of such secondary metabolites in callus and cell suspension cultures. Results: The results obtained indicated that both calli and cell suspension cultures were able to synthesize the target active ingredients and that cell suspension culture was superior to the callus culture in the biosynthesis and accumulation processes. By the end of the incubation period, the amount of total saponins in cell suspension culture reached up 51.97±0.26 dry biomass compared to 35.02 ±0.06 mg/g in callus culture. The amount of total flavonoids in cell suspension culture reached up 10.88±0.24 dry biomass compared to 6.40±0.02 mg/g in callus culture and of total glycosides reached up 6.11±0.25 dry biomass compared to 5.06 ±0.05 mg/g in callus culture. Conclusions: The results obtained in this study may indicate the promising role that plant cell culture will play in the future in phytopharmaceutical industry.                          Peer Review History: Received 16 July 2020; Revised 12 August; Accepted 26 August, Available online 15 September 2020 Academic Editor: Dr. Iman Muhammad Higazy, National Research Center, Egypt, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. Gehan Fawzy Abdel Raoof Kandeel, Researcher at pharmacognosy Department, National Research Centre, Egypt, [email protected] Ahmad Najib, Department of Pharmacognosy-Phytochemystry Universitas Muslim Indonesia-Indonesia, [email protected] Similar Articles: PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY OF COLOCASIA ESCULENTA (TARO) MEDICINAL PLANT LEAVES USED IN FOLK MEDICINE FOR TREATMENT OF WOUNDS AND BURNS IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEMEN PHYTOCHEMICAL SCREENING AND THIN LAYER CHROMATOGRAPHIC OF PRUNUS DULCIS (ALMOND) MEDICINAL PLANT LEAVES USED IN FOLK MEDICINE FOR TREATMENT OF WOUNDS AND BURNS IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEME