Evaluation of the effects of ozone therapy in the treatment of intra-abdominal infection in rats

INTRODUCTION: The antibacterial effect of ozone (O3) has been described in the extant literature, but the role of O3 therapy in the treatment of certain types of infection remains controversial. OBJECTIVES: To evaluate the effect of intraperitoneal (i.p.) O3 application in a cecal ligation/puncture rat model on interleukins (IL-6, IL-10) and cytokine-induced neutrophil chemoattractant (CINC)-1 serum levels, acute lung injury and survival rates. METHODS: Four animal groups were used for the study: a) the SHAM group underwent laparotomy; b) the cecal ligation/puncture group underwent cecal ligation/puncture procedures; and c) the CLP+O2 and CLP+O3 groups underwent CLP+ corresponding gas mixture infusions (i.p.) throughout the observation period. IL-6, CINC-1 and IL-10 concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Acute lung injury was evaluated with the Evans blue dye lung leakage method and by lung histology. P<0.05 was considered significant. RESULTS: CINC-1 was at the lowest level in the SHAM group and was lower for the CLP+O3 group vs. the CLP+O2 group and the cecal ligation/puncture group. IL-10 was lower for the SHAM group vs. the other three groups, which were similar compared to each other. IL-6 was lower for the SHAM group vs. all other groups, was lower for the CLP+O3 or CLP+O2 group vs. the cecal ligation/puncture group, and was similar for the CLP+O3 group vs. the CLP+O2 group. The lung histology score was lower for the SHAM group vs. the other groups. The Evans blue dye result was lower for the CLP+O3 group vs. the CLP+O2 group and the cecal ligation/puncture group but similar to that of the SHAM group. The survival rate for the CLP+O3 group was lower than for the SHAM group and similar to that for the other 2 groups (CLP and CLP+O2). CONCLUSION: Ozone therapy modulated the inflammatory response and acute lung injury in the cecal ligation/puncture infection model in rats, although there was no improvement on survival rates

Linearization of the Bradford protein assay to application in cow milk proteins quantification by UV-Vis spectrophotometry method.

Reliable methods for determination and quantification of total protein in food are essential information to ensure quality and safety of food trade. The objective of this study was to evaluate the linearity of calibration curves obtained from different proteins (blood serum albumin-BSA, α-LA, β-LG, caseins (CN): αs, β and κ-CAS) with the reagent of Bradford. Comercial UHT skimmed bovine milk was analyzed for the determination of total protein using the Bradford method by reading at 595 nm. The determination of the concentrations of total milk protein was achieved by linear regression. The Bradford method showed a high sensitivity for the determination of total proteins in bovine milk dilution 1:25 to values closer to those obtained by the Kjeldahl method. The results showed that the calibration curve of standard proteins β-CN and BSA obtained better linearity with less variation in the absorbance measurements for the determination of total protein of milk

Initial development of maize plants grown with different combinations of nitrate and ammonium

Corn is one of the main commodities of Brazilian agribusiness, due to its wide use, whether for human or animal consumption or in the chemical and biofuel industries. Thus, this work was carried out to evaluate the influence of different proportions of nitrate and ammonium in the initial development of corn plants. The experiment took place in a greenhouse, by direct sowing, where the seeds were placed at a depth of 1 cm in plastic pots with a capacity of 6 dm3 containing a mixture of previously sieved and washed with vermiculite, in a 2:1 ratio. Five proportions of nitrate and ammonium ions (NO3- :NH4+) were used: T1=100:0, T2=75:25, T3=50:50, T4=25:75 and T5=0:100, through nutrient solutions. After 35 days of cultivation, the following were evaluated: plant height, number of leaves, culm diameter, chlorophyll a, b, total and a/b ratio, root length, leaf fresh matter mass, dry matter mass of leaves, culm, roots, total dry mass, and root dry mass/shoot dry mass ratio, leaf area, specific leaf area, leaf area ratio, and leaf mass ratio. Nitrate as the only source of nitrogen provided a smaller increase in the chlorophyll a index of maize plants, which is the most important chlorophyll in the capture of light in the antenna complex. It is not recommended to cultivate corn plants with only nitrate or ammonium; however, it was observed that high doses of nitrate combined with low doses of ammonium in the culture medium provided satisfactory performance for most variables

Adaptability and stability of elite lineages and common bean cultivars of special grains in different seasons of cultivation in Minas Gerais, Brazil.

This work aimed to identify elite lineages and cultivars of special grains with better agronomic performance, adaptability and productivity stability at different growing seasons in the state of Minas Gerais, Brazil

Small forest losses degrade stream macroinvertebrate assemblages in the eastern Brazilian Amazon

Generally, habitat loss and fragmentation negatively affect biota, often in nonlinear ways. Such nonlinear responses suggest the existence of critical limits for habitat loss beyond which taxa experience substantial changes. Therefore, we identified change points for aquatic macroinvertebrate assemblages at both local-riparian and catchment extents in response to a forest-loss gradient in agriculture-altered landscapes of 51 small (1st to 3rd Strahler order) eastern Amazon streams. We used Threshold Indicator Taxa Analysis (TITAN) to identify change points for individual taxa and segmented regression analysis for assemblage richness. Considering the patterns of the cumulative frequency distributions of sum(Z−) maxima across bootstrap replications, peak changes in macroinvertebrate assemblages were at ∼9% (5–95 percentiles = 1–15%) of forest-loss at the catchment extent, and at ∼1.4% (5–95 percentiles = 0–35%) of forest-loss at the local-riparian extent. Although the assemblage change point at the site extent was less than that detected at the catchment extent, the markedly lower percentile range indicates that biotic assemblages are more clearly responsive to forest-loss at the catchment/network-riparian extents than the site extent. For catchment and site extents, segmented regression analysis determined a change point for assemblage richness at 57% and 79% of forest-loss, respectively. This indicates the low capacity of total richness to separate early and synchronous decreases of sensitive taxa from gradual increases of tolerant taxa. Our results also show that it is not enough to focus management and conservation actions on riparian zones, but that conservation strategies should be expanded to entire catchments as well. The sharp decline of sensitive taxa in response to removal of a small portion of forest cover, even at catchment extents, indicates that the Brazilian Forest Code is insufficient for protecting stream macroinvertebrates. Consequently, we recommend strategies to reverse the potential collapse of aquatic biodiversity, particularly through avoiding deforestation and forest degradation, encouraging socio-economic incentives for restoring degraded areas, creating protected areas, and maintaining the current protected areas. We argue that reducing habitat loss should be a top priority for conservation planners in tropical forests because the sensitivity of aquatic biodiversity to removal of riparian forest-cover in Amazon rainforests is higher than previously thought. Therefore, the Forest Code regulatory framework needs complementary regulation that may be achived by more restrictive State and biome policies. © 2019 Elsevier Lt

Assembléias de Formicidae da serapilheira como bioindicadores da conservação de remanescentes de Mata Atlântica no extremo sul do Estado da Bahia

In the extreme south of Bahia State, the Atlantic rainforest has been strongly affected by anthropogenic actions for a long time. Ants are seen as good biological indicators because of their abundance and sensitivity to changes of environmental conditions, thus allowing the evaluation of environmental impacts. The purpose of this study was to evaluate the environmental quality in four Atlantic Forest remnants through the study of Formicidae assemblages. Four reserves sampled during the experiment were: the school of agriculture EMARC (CEPLAC) in Teixeira de Freitas; Vista Alegre Farm in Alcobaça; FIBRASA Farm in Itamarajú; and Riacho das Pedras Farm in Prado. In each area, 50 units of one square meter of litter were sampled at intervals of 50m and 100m of the border using “Winkler” traps. The most frequent ants in EMARC reserve were: Wasmannia auropunctata (60%) and Solenopsis sp.1 (58%); in FIBRASA farm: W. auropunctata (64%) and Solenopsis sp.1 (46%); in Riacho das Pedras farm: Pyramica eggersi (80%) and Solenopsis sp.1 (64%); in Vista Alegre farm: Pheidole sp.1 (34%), Hypoponera sp.8 (32%) and Pyramica denticulata (24%). The highest value of diversity index (Shannon-Weaver) was observed in FIBRASA farm (3.41), followed by Riacho das Pedras farm (3.31), Vista Alegre farm (3.00), and EMARC reserve (2.33). The largest number of species was observed in FIBRASA and in Riacho das Pedras farms (55 species), followed by Vista Alegre (33) and EMARC (22). The area with the highest estimated diversity is the FIBRASA farm, while the reserve of EMARC had the lowest estimate. There were correlations between the level of human impact, the species found and generic richness, as well as with other ecological variables. All the studied areas had a high occurrence of species typical of degraded lands, showing that urgent conservation measures are needed to the increasingly more rare remnants of forest in the extreme South Bahia State.No extremo sul do Estado da Bahia, a Mata Atlântica tem sido fortemente afetada pelas atividades humanas. As formigas são vistas como indicadores biológicos confiáveis por serem sensíveis às mudanças das condições do ambiente, permitindo uma avaliação do impacto ambiental. O objetivo do presente estudo foi de avaliar a qualidade ambiental em quatro remanescentes de Mata Atlântica através de uma análise da estrutura das assembléias de Formicidae. As quatro reservas estudadas, todas situadas naquela região, foram: reserva da escola agrícola EMARC, Município de Teixeira de Freitas; Fazenda Vista Alegre, Alcobaça; Fazenda FIBRASA, Itamarajú e Fazenda Riacho das Pedras, Prado. Em cada área, foram amostrados 50 pontos de um metro quadrado de serapilheira, com o auxílio de armadilhas de tipo “Winkler”. O maior número de espécies observado foi na Fazenda FIBRASA (55 espécies) e na Fazenda Riacho das Pedras, seguidas pela Fazenda Vista Alegre (33) e a reserva da EMARC (22). A área de maior diversidade de espécies esperada (estimador Chao2) é também a da Fazenda FIBRASA, enquanto a área da EMARC foi também a de menor diversidade esperada. As espécies mais freqüentes na reserva da EMARC foram Wasmannia auropunctata (60% das amostras) e Solenopsis sp1 (58%); na Fazenda FIBRASA, W. auropunctata (64%) e Solenopsis sp.1 (46%); na Fazenda Riacho das Pedras, Pyramica eggersi (80%) e Solenopsis sp.1 (64%); na Fazenda Vista Alegre, Pheidole sp.1 (34%), Hypoponera sp.8 (32%) e Pyramica denticulata (24%). Existem correlações entre o nível de impacto humano e as riquezas específica e genérica, assim como com outras variáveis ecológicas, nas áreas estudadas. Todas essas apresentaram uma ocorrência elevada de espécies típicas de ambientes degradados, como é o caso de W. auropunctata, mostrando a urgência que existe numa política de conservação mais efetiva dos cada vez mais raros remanescentes do extremo sul do Estado da Bahia

Estudo atualiza nível de controle da bicheira-da-raiz em arroz.

A bicheira-da-raiz, denominação das larvas do gorgulho-aquático Oryzophagus oryzae, dependendo do nível de infestação pode ocasionar perdas econômicas de produtividade da cultura do arroz irrigado por inundação. Frente a isso, a Embrapa/UFPel, no âmbito do projeto ?Melhorarroz?, revisaram índices de perda de produção de arroz associados a distintos níveis de infestação larval, visando atualizar o nível de controle

Potencial genético de feijão-carioca para produtividade, resistência a doenças e qualidade comercial dos grãos.

O objetivo deste trabalho foi avaliar o potencial genético de linhagens-elite de feijão-carioca para produtividade, peso e tamanho dos grãos, adaptação à colheita mecanizada e tolerância às principais doenças que acometem a cultura

Practical Science and Environmental Education Workshop in Manaus, Brazil

It is an unequivocal fact that Amazonian tropical forest is the largest remaining primary forest in the world. The ecosystem in the region is e tremely comple with high biodiversity (Peres et al. 2010). Conservation and protection of the dynamic forest and river regions is e tremely important not only for the natural environments, but also for the economy and social dependence of benefits from such abundant natural environments. Important natural parameters that affect status of the natural environments include light (natural sunlight), soil, and water, which abundantly e ist in the Amazon region. Solar energy is the primary energy source for the majority of living organisms in both terrestrial and aquatic ecosystems, and drives the diurnal and seasonal cycles of biogeochemical processes (Monteith & Unsworth 2013). In particular, in situ light data remains one of the most underappreciated data measurements although having a significant impact on the physical, chemical and biological processes in the ecosystem (Johnsen 2012). Soil provides the fundamental basis for all terrestrial living organisms including the Amazonian forests as well as life-sustaining infrastructure for human society. Water is the most essential single entity to constitute all organisms from a single cell to the earth. Understanding of importance and roles of each factor and interaction of such comple dynamics in the natural environments can serve as fundamental platform for natural scientists, particularly for young scientists such as university students. The objective of this workshop was to provide hand- on scientific and environmental education for university students in Manaus, Amazonas, Brazil through practical field measurements using the three most important parameters in the natural ecosystem composed of natural sunlight, soil, and water. The workshop was divided into a series of lectures, in situ field sampling, and data processing, analysis and interpretation with the ultimate goal of empowering the undergraduate students with research-centered environmental education and e perience of developing international collaboration.departmental bulletin pape

La pobreza del Estado : reconsideración del rol del Estado en la lucha contra la pobreza global

El espectro de la pobreza ha tenido una presencia constante en la historia de la humanidad. Actualmente es posible erradicar la pobreza extrema. Existen los recursos para lograrlo en un período razonable. Los deseos y la disposición de las organizaciones internacionales, los gobiernos y los pueblos son evidentes en los discursos dominantes que expresan la necesidad y la voluntad de la comunidad internacional de reducir y erradicar la pobreza. ¿Cuáles son los factores que están impidiendo el logro de una meta tan ampliamente aceptada? Es difícil dar una respuesta exhaustiva y precisa a esta pregunta. No obstante, una parte sustancial de la explicación puede estar en una de las estructuras más importantes, pero problemáticas, del mundo moderno, a saber, el Estado.Prefacio / Hartley Dean, Alberto Cimadamore y Jorge Siqueira Introducción Parte I. Dimensiones teórica y global El programa mundial de derechos humanos y la (im)posibilidad del Estado ético Hartley Dean La redefinición del problema social y la retórica acerca de la pobreza durante el decenio de 1990 Anete Brito Leal Ivo La antiglobalización y el antiestatismo desde la perspectiva del postdesarrollo y el antiutilitarismo Paulo Henrique Martins Parte II. Dimensiones política y nacional La estrategia de reducción de la pobreza y la brecha educacional en la Guatemala de posguerra Virgilio Álvarez Aragón El futuro que no llegará: la erradicación de la pobreza desde la perspectiva del gobierno federal de México (2000-2006) Nelson Arteaga Botello Las dificultades de una política de seguridad alimentaria en el Brasil José Graziano da Silva, Walter Belik y Maya Takagi Políticas de empleo público como instrumentos para la reducción de la pobreza y la desigualdad en el Brasil Tarcisio Patricio de Araujo y Roberto Alves de Lima Pobreza, desigualdad y desarrollo: el rol del Estado en la experiencia de Cuba Mayra Paula Espina Prieto ¿Privatización para la reducción de la pobreza? : El caso de Bangladesh y su importancia para otras economías en desarrollo Hulya Dagdeviren Parte III. Dimensiones práctica y local Sinergias frágiles para el desarrollo: el caso de Jardim Oratório, São Paulo, Brasil Rowan Ireland Pobreza, políticas y prestidigitación en Bolivia y América Latina John-Andrew McNeish Fondos Sociales en África: ¿una respuesta clientelístico-tecnocrática a la pobreza? Einar Braathe