Rhizobial diversity from stem and root nodules of Discolobiumand Aeschynomene.

Among the genera that exhibit stem nodulation, Aeschynomeneand Discolobiumare found in the Pantanal Mato-grossense (wetlands in the state ofMato Grosso, Brazil). Isolates obtained from the stem and root nodules of D. pulchellum, D. psoraleaefolium, D. leptophyllumand A. fluminensiswere collected from various locations in the Pantanal de Poconésub-region and phenotypically characterized and genotyped by restriction analysis of the 16S rDNA gene. Of the 282 isolates obtained from the stem and root nodules, 84.3% alkalized YMA media and 74.1% showed slow growth. No differences in either the phenotype or the genotype among the rhizobial populations isolated from the root or stem nodules of the species Discolobiumand A. fluminensis were observed. Among the isolates obtained from D. pulchellum,there was a group that was not similar to any of the reference strains used, and most of the isolates analyzed by PCR-RFLP were similar to the genus Bradyrhizobium

Avaliação de estirpes nativas de rizóbio para inoculação em feijão-caupi.

JIPE 2013

Efeito da interação genótipo x ambiente no ganho esperado com seleção em feijoeiro-comum.

O objetivo do presente trabalho foi avaliar o efeito da interação genótipos x ambientes e o reflexo do ganho com a seleção em determinados ambiente, por meio da seleção praticada em outros ambientes, para produtividade de grãos.CONAF

Weight evaluation of Tabapuã cattle raised in northeastern Brazil using random-regression models - Avaliação do peso de bovinos Tabapuã criados no Nordeste do Brasil utilizando-se modelos de regressão aleatória.

Na publicação: D. M. AZEVÊDO; S. Sousa Júnior

Acceptance of the bodypainting as supportive method to learn the surface locomotor apparatus anatomy of the horse

Although bodypainting has been reported as a great resource for teaching surface anatomy of humans, its use in veterinary anatomy has not been scientifically reported. In the present study, bodypainting was performed on 4 horses for anatomy teaching purposes of the equine locomotor apparatus. We aimed to use the bodypainting method as an additional tool to classic teaching and to test the relevance of our purpose. Twenty one Brazilian veterinary students were given a 90-min session, which included a presentation of painted horses, with opportunities for the students to ask questions and to palpate anatomic locations on the horses. Based on a questionnaire, there was unanimous student satisfaction with this technique. Furthermore, student scores on practical tests to evaluate the attention retain given immediately before and 1 h after the session were 33.9 ± 19.8% and 69.0 ± 18.4%, respectively (p < 0.001). We concluded that bodypainting has great potential for support the classic lectures of the equine locomotor apparatus

Efeito de diferentes fontes energéticas da dieta sobre a produção de metano utilizando a técnica de fermentação ruminal ex situ (microrúmen).

O objetivo do experimento foi avaliar o efeito de diferentes fontes energéticas da dieta sobre a fermentação ruminal em bovinos. O delineamento adotado foi o quadrado latino 3x3 replicado, com período de 16 dias e 3 tratamentos: Controle: Dieta de baixo extrato etéreo; Soja: Dieta de alto extrato etéreo (inclusão de 15% de soja grão); Polpa: Dieta de baixo extrato etéreo e alta participação de pectina (inclusão de 15% de polpa cítrica). As coletas de conteúdo ruminal foram realizadas no 16º dia experimental, sendo realizadas antes, 3, 6, 9 e 12 horas após a alimentação matinal. Utilizou-se a técnica de fermentação ruminal ex situ, a qual consiste em incubar frascos de penicilina contendo conteúdo ruminal sólido e líquido em banho termostático por 30min. Após a incubação era realizada a mensuração de metano e ácidos graxos de cadeia curta por cromatografia gasosa. A partir desta técnica consegue-se estimar a produção de ácidos graxos de cadeia curta, metano e a perda de energia realtiva (PER). A PER estima a eficiência da fermentação dos alimentos, ou seja, com ela é possível avaliar a perda de metano em relação aos outros produtos da fermentação. A Polpa aumentou a produção de acético e butírico e a dieta Soja diminuiu os mesmos ácidos graxos de cadeia curta. Nenhuma das dietas alterou a produção de metano ou a perda de energia relativa ao metano

Orbit determination of Transneptunian objects and Centaurs for the prediction of stellar occultations

The prediction of stellar occultations by Transneptunian objects and Centaurs is a difficult challenge that requires accuracy both in the occulted star position as for the object ephemeris. Until now, the most used method of prediction involving tens of TNOs/Centaurs was to consider a constant offset for the right ascension and for the declination with respect to a reference ephemeris. This offset is determined as the difference between the most recent observations of the TNO and the reference ephemeris. This method can be successfully applied when the offset remains constant with time. This paper presents an alternative method of prediction based on a new accurate orbit determination procedure, which uses all the available positions of the TNO from the Minor Planet Center database plus sets of new astrometric positions from unpublished observations. The orbit determination is performed through a numerical integration procedure (NIMA), in which we develop a specific weighting scheme. The NIMA method was applied for 51 selected TNOs/Centaurs. For this purpose, we have performed about 2900 new observations during 2007-2014. Using NIMA, we succeed in predicting the stellar occultations of 10 TNOs and 3 Centaurs between 2013 and 2015. By comparing the NIMA and JPL ephemerides, we highlighted the variation of the offset between them with time. Giving examples, we show that the constant offset method could not accurately predict 6 out of the 13 observed positive occultations successfully predicted by NIMA. The results indicate that NIMA is capable of efficiently refine the orbits of these bodies. Finally, we show that the astrometric positions given by positive occultations can help to further refine the orbit of the TNO and consequently the future predictions. We also provide the unpublished observations of the 51 selected TNOs and their ephemeris in a usable format by the SPICE library.Comment: 12 pages, 9 figures, accepted in A&