Novel galanin receptors in teleost fish: identification, expression and regulation by sex steroids

In fish, the onset of puberty, the transition from juvenile to sexually reproductive adult animals, is triggered by the activation of pituitary gonadotrophin secretion and its timing is influenced by external and internal factors that include the growth/adiposity status of the animal. Kisseptins have been implicated in the activation of puberty but peripheral signals coming from the immature gonad or associated to the metabolic/nutritional status are also thought to be involved. Additionally, there is evidence that the galaninergic system in the brain and testis of pre-pubertal male sea bass is a possible mediator involved in the translation of somatic signals leading to gonadal maturation. Here, the transcripts for four galanin receptors (GALR), named GALR1a, 1b, 2a and 2b, were isolated from European sea bass, Dicentrarchus labrax. Phylogenetic analysis confirmed the previously reported duplication of GALR1 in teleost fish, and unravelled the duplication of GALR2 in teleost fish and in some tetrapod species. Comparison with human showed that the key amino acids involved in ligand binding are present in the corresponding GALR1 and GALR2 orthologues. Transcripts for all four receptors are expressed in brain and testes of adult fish with GALR1a and GALR1b abundant in testes 15 and hardly detected in ovaries. In order to investigate whether GALR1 dimorphic expression was dependent on steroid context we evaluated the effect of 11-ketotestosterone and 17β-estradiol treatments on the receptor expression in brain and testes of pre-pubertal males. Interestingly, steroid treatments had no effect on the expression of GALRs in the brain while in the testes, GALR1a and GALR1b were significantly up regulated by 11KT. Altogether, these results support a role for the galaninergic system, in particular the GALR1 paralog in fish reproductive function

COVID-19 and the targets of SDG 8: reflections on Brazilian scenario

Purpose: This paper aims to present reflections on the COVID-19 pandemic impacts in the search for the targets of Sustainable Development Goal 8 (SDG 8) disseminated by the United Nations. These reflections are made considering the Brazilian scenario. Design/methodology/approach: As this paper is a viewpoint, several information presented here are based on authors’ opinion. However, to enhance the information basis and present examples, a bibliographic research was also conducted. Findings: Analyzing the targets of SDG 8 and the probable crisis in the Brazilian economy as a consequence of the pandemic, the authors believe that this crisis will impact the achievement of these targets. Practical implications: The reflections presented here can contribute with the debates about the search for decent work in Brazil. It is worth to remember that the development of actions aligned with SDG 8 targets generates more than only economic gains, as these contribute for building a more just, egalitarian and inclusive society. Originality/value: This viewpoint calls attention to issues that demand urgent public policies; otherwise, these issues can generate losses to an entire generation of Brazilians

Difficulties observed in hydroelectric turbine projects management: evidence from case studies

Purpose: This study aims to analyze the difficulties observed in the management of three hydroelectric turbine projects developed by a large multinational company in the metal-mechanic segment, identifying those most critical. Design/methodology/approach: Through a bibliographic search, difficulties throughout project management were identified. These difficulties were used as a research protocol to carry out three case studies in a hydroelectric turbine manufacturer. The projects selection aimed to contemplate different characteristics to encompass the diversity of their typologies. Data were analyzed through content analysis technique and the difficulties were ordered via Grey Relational Analysis (GRA). Findings: Qualitative data analysis provided specific insights regarding the characteristics of each project. When analyzing the difficulties of hydroelectric turbine projects management via GRA, it is observed that those difficulties most evidenced were related to integration and communication, suppliers management and objectives definition. Originality/value: The main contribution of this research lies in the sector under analysis. The literature about hydroelectric turbine projects is scarce. In addition, no similar studies were found in the literature

Sustainable procurement practices in the supplier selection process: an exploratory study in the context of Brazilian manufacturing companies

Purpose: This paper aims to evaluate the adoption of sustainable procurement practices adopted by Brazilian manufacturing companies in supplier selection; additionally, it is aimed to understand which of these practices enable a better differentiation of the analysed companies. Design/methodology/approach: A systematic literature review was performed to compose the theoretical base of this research. In addition, a detailed study of ISO 20400 standard was conducted. The guidelines of ISO 20400 were used as a base to structure a questionnaire used in a survey with professionals working in procurement sphere of manufacturing companies in Brazil. The data were analysed via frequency and CRITIC (Criteria Importance Through Intercriteria Correlation) method. Findings: A moderate dispersion in the adoption level of sustainable procurement practices in supplier selection process of the manufacturing companies was observed; in practices associated with social aspects, the dispersion is greater. A negative issue to be highlighted is that almost 20% of analysed companies did not even considered in their supplier selection process if their candidates accomplish philanthropic activities, generate jobs in local community and fulfill the Universal Declaration of Human Rights of United Nations (UN). Those two last practices are the ones with the best capacity to differ the companies in the sample. Originality/value: There are few studies that focuses on understanding the adoption of sustainable procurement practices in manufacturing companies' supplier selection process. The main contribution of this study to the literature is to evidence that social requirements in supplier selection process are considered in a clear and well-structured form only by few Brazilian manufacturing companies. Despite the sample size, companies analysed in this research are prominent organisations in manufacturing sector. Thus, if this situation occurs in these companies, a more critical scenario will be evidenced in other organisations. This study has implication for practice and academy. For companies' managers, information present here can be used to debate the theme in the organisational context and the nine practices and scale can be used to perform a critical analysis of company's practices. For researchers, the information present here can be used as starting point for futures studies

Elevated serum levels of soluble CD154 in children with juvenile idiopathic arthritis

<p>Abstract</p> <p>Objective</p> <p>Cytokines play important roles in mediating inflammation in autoimmunity. Several cytokines are elevated in serum and synovial fluid samples from children with Juvenile Idiopathic Arthritis (JIA). Soluble CD154 (sCD154) is elevated in other autoimmune disorders, but has not been characterized in JIA. Our objectives were to determine if sCD154 is elevated in JIA, and to examine correlations between sCD154 and other inflammatory cytokines.</p> <p>Methods</p> <p>Serum from 77 children with JIA and 81 pediatric controls was analyzed for interleukin (IL)1β, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, sCD154, interferon-γ (IFNγ), soluble IL2 receptor (sIL2R), and tumor necrosis factor-α (TNFα), using the Luminex Multi-Analyte Profiling system. Differences in levels of cytokines between cases and controls were analyzed. Logistic regression was also performed.</p> <p>Results</p> <p>sCD154 was significantly elevated in cases compared to controls (p < 0.0001). IL1β, IL5, IL6, IL8, IL13, IFNγ, sIL2R, and TNFα were also significantly elevated in JIA. Levels of sCD154 were highly correlated with IL1β, IL6, IL8, and TNFα (p < 0.0001). Logistic regression analysis suggested that IL6 (odds ratio (OR): 1.4, p < 0.0001), sCD154 (OR: 1.1, p < 0.0001), and TNFα (OR: 1.1, p < 0.005) were positively associated with JIA, while IL10 (OR: 0.5, p < 0.002) was protective. sCD154 was elevated in all JIA subtypes, with highest levels among more severe subtypes. IL1β, IL6, IL8, sIL2R and TNFα were also elevated in several JIA subtypes.</p> <p>Conclusion</p> <p>Serum levels of sCD154, IL1β, IL6, IL8, sIL2R and TNFα are elevated in most JIA subtypes, suggesting a major role for sCD154, and these cytokines and cytokine receptors in the pathogenesis of JIA.</p

Dupla inoculação (rizóbio e fungos micorrízicos arbusculares) mitigam efeitos nocivos da salinidade no crescimento do feijão-caupi

A salinidade em áreas áridas e semiáridas do mundo está a expandir rapidamente devido às alterações climáticas e atividades humanas. O uso de inóculos contendo microrganismos benéficos, como fungos micorrízicos arbusculares (FMA) e bactérias simbióticas fixadoras de azoto, aliado ao manejo adequado do solo e das culturas, é uma alternativa promissora para melhorar a produção vegetal nessas regiões. Aqui, investigámos o efeito de práticas agrícolas comuns, nomeadamente a utilização de microrganismos benéficos como inóculo e a perturbação do solo entre a rotação de culturas, no crescimento do feijão-caupi e na sua associação com micróbios do solo sob condições salinas e não salinas. A estirpe Bradyrhizobium yuanmingense BR 3267, e uma mistura comercial de FMA (Endoplant Riego) foram utilizados como inóculos. Paralelamente, avaliámos o crescimento do feijão-caupi, após sucessão ao capim-buffel com ou sem perturbação prévia do solo. Ensaios de plantas em solo não-esterilizado (suplementado ou não com NaCl) foram realizados em dois ciclos de 75 dias em estufa. Parâmetros vegetais e simbióticos e o teor de nutrientes nas folhas foram determinados, bem como a diversidade bacteriana e de FMA em raízes e nódulos de feijão-caupi por meio de sequenciamento DNA metabarcode. Os nossos dados revelaram que os parâmetros simbióticos (número de nódulos e/ou taxa de colonização) foram melhorados no feijão-caupi inoculado com Bradyrhizobium e/ou FMA comercial, o que consequentemente resultou em maior teor de N ou P nas folhas. A salinidade imposta afetou negativamente o crescimento do feijão, porém sem afetar significativamente os parâmetros simbióticos analisados. O aumento de propágulos de FMA disponíveis no solo através da inoculação de FMA comercial foi um fator determinante para mitigar os efeitos do manejo do solo no crescimento do feijão. A perturbação do solo mostrou impacto negativo nos parâmetros vegetais e simbióticos, exceto para o número de nódulos. Além disso, os efeitos positivos do uso de FMA comercial no crescimento do feijão-caupi, em condições controlo ou de salinidade podem ser explicados pelas mudanças na funcionalidade das comunidades de FMA associadas ao feijão-caupi. Por outro lado, as comunidades bacterianas em nódulos ou raízes do feijão não foram afetadas pela inoculação de FMA ou de rizóbios. Este estudo revela que a dupla inoculação (mistura de rizóbio e FMA) e a rotação de culturas, sem a perturbação do solo, são práticas eficazes para melhorar o crescimento do feijão-caupi e mitigar os efeitos nocivos do sal no seu crescimento. Mais, estes resultados também sugerem que os efeitos sinérgicos entre rizóbios e FMA dependem principalmente de um estabelecimento bem-sucedido da simbiose entre FMA e a planta hospedeira e não da comunidade de FMA em si

Towards the SDGs for gender equality and decent work: investigating major challenges faced by Brazilian women in STEM careers with international experience

This paper aims to understand the main difficulties faced by women throughout their careers in Brazil and abroad. Based on the information gathered from these experiences, it seeks to advance the discussion on women's participation in STEM focusing on SDG 5 (gender equality) and SDG 8 (decent work). The main difficulties experienced by women in STEM as discussed in the academic literature were mapped. This provided input to develop a questionnaire containing qualitative and quantitative questions used to conduct interviews with women working in STEM. The sample consisted of highly qualified professionals working in high positions in the hierarchies of multinational companies in the STEM field with experience both in Brazil and abroad. The data collected was analyzed using a mixed-methods approach, including content analysis for qualitative questions and the Grey Relational Analysis for quantitative questions. The results revealed that the lack of flexible work systems, the scarcity of gender-sensitive organizational policies and labor policies, and the prevalence of traditional cultural models are some of the main difficulties faced both in Brazil and abroad by the women interviewed. The need to discuss issues of gender equality and decent work in the early stages of education is important for increasing women’s participation in STEM, which is a critical factor in the development of inclusive organizations and in fully achieving the sustainable development of society. This paper presents a unique perspective of the perceived difficulties faced by executive women who worked in Brazil and in different countries (i.e., Canada, Denmark, France, Germany, Switzerland and the United States). Gender equality in organizations is highly context-dependent, and cross-cultural analysis generates relevant insights to face the challenges and advance the discussion on women’s participation in STEM

Brazilian contributions to the Sustainable Development Goal 7 and policy implications

Purpose: The seventh United Nations' Sustainable Development Goal (SDG 7) is broadly characterised by ensuring affordable and clean energy for all. Considering the need for research associated with SDGs in developing countries, the Brazilian economy importance and its energy matrix, the purpose of this study is to evaluate how Brazil has been acting in favour of 7th SDGs targets. Design/methodology/approach: The methodological strategy used was a survey conducted with 42 energy planning specialists who know the Brazilian reality. Data were analysed via averages and the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) technique. Findings: The results show that, in general, the actions taken by Brazil to achieve the goals related to SDG 7 have been developed in a simply and punctually form, needing to move considerably towards developing more planned activities; with this, satisfactory and consolidated results are expected on a national scale. Originality/value: The information presented can significantly contribute to the broadening of debates associated with sustainable development and energy planning, for both academic and political-economic spheres. Information present here can support future more profound studies, and these, in turn, support the Brazilian government in better actions to achieve the SDG 7 targets

Critical analysis of engineering education focused on sustainability in supply chain management: an overview of Brazilian higher education institutions

© 2020, Emerald Publishing Limited. Purpose: This paper aims to critically analyze the engineering education focused on sustainability in supply chain management, in courses offered by Brazilian higher education institutions. Design/methodology/approach: Topics related to sustainable supply chain management were listed from the literature and used as a framework to gather professors’ opinions on how well these topics are covered in engineering courses offered in Brazil. Data analysis was performed via frequency analysis and comparative ordering using the Fuzzy technique for order preference by similarity to ideal solution technique. Findings: It was possible to evidence that most of the topics are superficially presented within other subjects and that there are few associated practical activities that enable greater learning. Comparatively, issues related to ISO standards (related to quality and environmental management systems) and compliance with environmental laws, regulations and standards were highlighted. Additionally, it was possible to verify that there is a need for further study on issues related to energy efficiency, worker training and corporate governance. Originality/value: No similar study was found in the literature. The findings presented in this paper can contribute to the improvement of engineering education in Brazil and other countries