Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats

NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. July 2010; 398 (2): 199-204.Conjugated linoleic acid (CLA) is a dietary fatty acid frequently used as a body fat reducing agent whose effects upon cell membranes and cellular function remain unknown. Obese Zucker rats were fed atherogenic diets containing saturated fats of vegetable or animal origin with or without 1% CLA, as a mixture of cis(c)9,trans(t)11 and t10,c12 isomers. Plasma membrane vesicles obtained from visceral adi- pose tissue were used to assess the effectiveness of dietary fat and CLA membrane incorporation and its outcome on fluidity and permeability to water and glycerol. A significant decrease in adipose membrane fluidity was correlated with the changes observed in permeability, which seem to be caused by the incor- poration of the t10,c12 CLA isomer into membrane phospholipids. These results indicate that CLA supple- mentation in obese Zucker rats fed saturated and cholesterol rich diets reduces the fluidity and permeability of adipose membranes, therefore not supporting CLA as a body fat reducing agent through membrane fluidification in obese fat consumers

Cape Verde: The Case for Euroization

After 10 years of a fixed exchange rate against the euro and a deepening integration with the European Union (EU), the authorities of Cape Verde maintain a strong commitment to nominal stability and are now considering the official euroization of the country. Compared to the current pegging, euroization could be costly if the economic conditions of Cape Verde were to require control over the interest rates and the exchange rate. Given the strong economic and financial integration between Cape Verde and Europe, and the fact that Cape Verde records inflation rates at levels that are similar to those of the European Monetary Union (EMU), the relevant issue is whether the European Central Bank (ECB) monetary policy fits the needs of Cape Verde. In order to answer this question, we empirically assess the synchronization between the business cycle of Cape Verde and the business cycle of the EMU. For that purpose, we compute output gaps and then use conventional correlation measures as well as other indicators recently suggested in the literature. Replicating the methodology for each of the current 27 EU members, our results show that Cape Verde ranks better than several EU countries and even better than some EMU countries. We thus argue that there is a strong case for the euroization of Cape Verde. Euroization would secure the benefits already attained with the pegging to the euro and would warrant additional benefits, most likely with no relevant costs stemming from inappropriate ECB monetary policies.Africa, Cape Verde, European Monetary Union, Euroization, Business Cycles

Understanding Water Equilibration Fundamentals as a Step for Rational Protein Crystallization

Background: Vapor diffusion is the most widely used technique for protein crystallization and the rate of water evaporation plays a key role on the quality of the crystals. Attempts have been made in the past to solve the mass transfer problem governing the evaporation process, either analytically or by employing numerical methods. Despite these efforts, the methods used for protein crystallization remain based on trial and error techniques rather than on fundamental principles. Methodology/Principal Findings: Here we present a new theoretical model which describes the hanging drop method as a function of the different variables that are known to influence the evaporation process. The model is extensively tested against experimental data published by other authors and considering different crystallizing conditions. Aspects responsible for the discrepancies between the existing theories and the measured evaporation kinetics are especially discussed; they include the characterization of vapor-liquid equilibrium, the role of mass transfer within the evaporating droplet, and the influence of the droplet-reservoir distance. Conclusions/Significance: The validation tests show that the proposed model can be used to predict the water evaporation rates under a wide range of experimental conditions used in the hanging drop vapor-diffusion method, with no parameter fitting or computational requirements. This model combined with protein solubility data is expected to become a usefu

Metabolic aspects of palladium(II) potential anti-cancer drugs

This mini-review reports on the existing knowledge of the metabolic effects of palladium [Pd(II)] complexes with potential anticancer activity, on cell lines and murine models. Most studies have addressed mononuclear Pd(II) complexes, although increasing interest has been noted in bidentate complexes, as polynuclear structures. In addition, the majority of records have reported in vitro studies on cancer cell lines, some including the impact on healthy cells, as potentially informative in relation to side effects. Generally, these studies address metabolic effects related to the mechanisms of induced cell death and antioxidant defense, often involving the measurement of gene and protein expression patterns, and evaluation of the levels of reactive oxygen species or specific metabolites, such as ATP and glutathione, in relation to mitochondrial respiration and antioxidant mechanisms. An important tendency is noted toward the use of more untargeted approaches, such as the use of omic sciences e.g., proteomics and metabolomics. In the discussion section of this mini-review, the developments carried out so far are summarized and suggestions of possible future developments are advanced, aiming at recognizing that metabolites and metabolic pathways make up an important part of cell response and adaptation to therapeutic agents, their further study potentially contributing valuably for a more complete understanding of processes such as biotoxicity or development of drug resistance.AG acknowledges funding from the CICECO-Aveiro Institute of Materials project, with references UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. MM acknowledges financial support from POCentro, COMPETE 2020, Portugal 2020 and European Community through the FEDER and the Portuguese Foundation for Science and Technology (UIDB/00070/2020). TC and AM are grateful to the Portuguese Foundation for Science and Technology (FCT) for grants SFRH/BD/145920/2019 and SFRH/BD/111576/2015, respectively.publishe

Social and cultural contexts change but intelligence persists as incisive to explain children’s academic achievement

The intelligence is a well established predictor of school achievement. Although school failure/success can be explained by cognitive variables, socio-familial variables can also have an impact. Since these variables haven’t been so systematically investigated together, the present study aims to consider both variables to understand their causal roles in academic achievement. With a sample of 376 Portuguese children aged 6 to 10 years, a path analysis was carried out based on a prior analysis to search for causal relationships between intelligence and socio-familial variables to explain children’s academic achievement. The results point to intelligence as a major influence on school performance, combined with socio-familial variables (directly: community, type of school, mother's education and school year; and indirectly: socioeconomic status and father’s education level). Practical implications were discussed concerning the relevance of the investigated variables in explaining academic achievement of children.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Fundação para a Ciência e Tecnologia, IP (FCT) and the POCH/FSE under Grant SFRH/BD/85110/2012 to Ana Filipa Alves

A comparison between on-site measured and estimated based adjustment factor values used to calculate heat losses to unconditioned spaces in dwellings

Steady-state methods have been widely used in Europe to analyse the energy performance of low-energy buildings. The accuracy of such methods depends on the assumptions regarding the compensation of non-stationary effects but also on the input design data, such as the temperature of unconditioned spaces (UnSp). This temperature depends mainly on the thermal characteristics of UnSp envelope, air ventilation rate, temperature of the conditioned spaces, and the external environment. External environment varies over time, daily and seasonally, making it difficult to accurately estimate UnSp temperature. European Union (EU) directives stated that the UnSp temperature can be evaluated by the adjustment factor (b) set by EN ISO 13789. However, each Member State may adjust procedures, by proposing simplified approaches, either for new or existing buildings. In this paper the b-values measured on-site in three dwellings were compared to those calculated by EN ISO 13789, as well as those estimated based on simplified procedures, allowed in the regulatory framework of some EU Member States, namely Ireland, Portugal, Spain, France, and Italy. The study allowed to conclude that EN ISO 13789 and Irish BR 443 provided similar values. However, if the purpose is to simplify procedures and reduce computation effort, French RE2020 proved to be very effective. The thermal characteristics of the UnSp envelope and air ventilation rate were identified as the parameters that most affect the estimation of the b-value, while thermal losses through linear thermal bridges and the ground do not seem to have a significant impact.info:eu-repo/semantics/publishedVersio