Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats

NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications. July 2010; 398 (2): 199-204.Conjugated linoleic acid (CLA) is a dietary fatty acid frequently used as a body fat reducing agent whose effects upon cell membranes and cellular function remain unknown. Obese Zucker rats were fed atherogenic diets containing saturated fats of vegetable or animal origin with or without 1% CLA, as a mixture of cis(c)9,trans(t)11 and t10,c12 isomers. Plasma membrane vesicles obtained from visceral adi- pose tissue were used to assess the effectiveness of dietary fat and CLA membrane incorporation and its outcome on fluidity and permeability to water and glycerol. A significant decrease in adipose membrane fluidity was correlated with the changes observed in permeability, which seem to be caused by the incor- poration of the t10,c12 CLA isomer into membrane phospholipids. These results indicate that CLA supple- mentation in obese Zucker rats fed saturated and cholesterol rich diets reduces the fluidity and permeability of adipose membranes, therefore not supporting CLA as a body fat reducing agent through membrane fluidification in obese fat consumers

Impacto das variáveis sociofamiliares no desenvolvimento cognitivo da criança

O presente estudo explora a variável meio (urbano vs rural) no desenvolvimento cognitivo das crianças. A investigação na área aponta que fatores sociofamiliares (profissão da mãe e do pai, habilitações escolares da mãe e do pai e meio de pertença urbano vs rural), assim como os contextos escolares, estão associados a diferenças nas habilidades cognitivas das crianças. Nesta comunicação recorremos à aplicação da Escala de Competências Cognitivas (ECCOs4/10) a uma amostra de crianças com idades compreendidas entre os 6 e os 9 anos, do 1º Ciclo do Ensino Básico, de escolas públicas e privadas, do meio rural e urbano. Esta aplicação da escala procurou analisar as reações e atitudes das crianças em relação a cada uma das provas e aos seus itens, no fundo um estudo mais qualitativo dos processos e estratégias cognitivas que as crianças usam na resolução da ECCOs.Fundação para a Ciência e a Tecnologia (FCT

Book of Abstracts of the 8th International Symposium on Delivery of Functionality in Complex Food Systems

This volume contains the abstracts presented at the 8th International Symposium on Delivery of Functionality in Complex Food Systems, held in Sheraton Porto Hotel Conference Centre, Porto, Portugal, 7-10 July, 2019.info:eu-repo/semantics/publishedVersio

Polyelectrolyte precipitation: a new green chemistry approach to recover value-added proteins from different sources in a circular economy context

Proteins have always been vital biological molecules used for industrial purposes, human nutrition and health. Nowadays, seeking new alternatives and sources of these biomolecules is becoming an increasing research trend derived from the present consumer awareness between food consumption and health promotion, but also on environmental sustainability. Although there are different consolidated/traditional downstream processes to obtain proteins, such as chromatography tools, alkali hydrolysis, precipitation by inorganic salts and organic solvents, their industrial-scale application still demands urgent innovation due to the poor recovery yields, high costs and time-consuming steps, environmental impact as well as some toxic concerns. Polyelectrolyte precipitation represents a green, innovative alternative for protein recovery; however, there are reduced data regarding its pilot or industrial-scale application. In this literature work, the action mechanism and principles with regards to its functionality and insights for its application on a big scale are reviewed. Overall, this review discusses the novelty and sustainability of protein precipitation by polyelectrolytes from different sources against traditional techniques as well as highlights the relationship between protein source, production relevance and bioactive properties that are key factors to maximize the application of this extractive method on a circular economy context.info:eu-repo/semantics/publishedVersio

Bioremediation of petroleum-contaminated soils: mathematical modelling as a tool for the simulation of alternative strategies

POCI-01-0145-FEDER-016575; ERC Grant n.º 323009; UID/BIO/04469/2013; POCI -01-0145-FEDER-006684; NORTE-01-0145-FEDER-000004; FCOMP-01-0124- FEDER-027462; SFRH/BPD/80528/2011info:eu-repo/semantics/publishedVersio

The development of gullies in a Mediterranean environment: The example of the Corgo gully (central Portugal)

ICEER2019 - 6th International Conference on Energy and Environment Research: “Energy and environment: challenges towards circular economy”, 22 -25 July 2019, Aveiro, PortugalGullies are the most energy-efficient way to transport excess runoff from the watershed after a landscape disturbance. The diversity of physical factors that are associated with gully formation makes straightforward interpretation difficult and requires well-founded analysis based on local observations. Some gullies have developed in the Alva river basin and some of them reach a spectacular size, especially in areas where forest fires have recurred with greater severity. In this paper, we identify the most important factors in the formation and development of the River Corgo’s gully, located in the Alva river valley in central Portugal. The evolution of this gully in the last 4 years is also examined, based on a study of the modification of its morphological characteristics. The analysis was based on the Spearman-Rho correlation coefficient and stepwise multiple regression to estimate the correlation between the quantitative characteristics, geomorphological processes and biophysical variables. The results show that the main factors that seem to control the spatial variation of soil erosion are the soil penetration resistance, slope, slope shape and vegetation cover.info:eu-repo/semantics/publishedVersio

Hydrocarbon toxicity towards hydrogenotrophic methanogens in oily waste streams

Hydrocarbon-containing wastes and wastewaters are produced worldwide by the activities of the oil and gas industry. Anaerobic digestion has the potential to treat these waste streams, while recovering part of its energy potential as biogas. However, hydrocarbons are toxic compounds that may inhibit the microbial processes, and particularly the methanogens. In this work, the toxicity of hexadecane (030 mM) towards pure cultures of hydrogenotrophic methanogens (Methanobacterium formicicum and Methanospirillum hungatei) was assessed. Significantly lower (p 30 mM for M. formicicum and M. hungatei, respectively, suggesting that toxic effects from aliphatic hydrocarbons towards hydrogenotrophic methanogens may not occur during anaerobic treatment. Hydrocarbon toxicity towards hydrogenotrophic methanogens was further assessed by incubating an anaerobic sludge with H2/CO2 in the presence of a complex mixture of hydrocarbons (provided by the addition of an oily sludge from a groundwater treatment system). Specific methanogenic activity from H2/CO2 decreased 1.2 times in the presence of the hydrocarbons, but a relatively high methane production (~30 mM) was still obtained in the assays containing the inoculum and the oily sludge (without H2/CO2), reinforcing the potential of anaerobic treatment systems for methane production from oily waste/wastewater.This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of project MORE (POCI-01-0145-FEDER-016575) and by the strategic funding of UIDB/04469/2020 unit.info:eu-repo/semantics/publishedVersio

Atypical moral judgment following traumatic brain injury

Previous research has shown an association between emotions, particularly social emotions, and moral judgments. Some studies suggested an association between blunted emotion and the utilitarian moral judgments observed in patients with prefrontal lesions. In order to investigate how prefrontal brain damage affects moral judgment, we asked a sample of 29 TBI patients (12 females and 17 males) and 41 healthy participants (16 females and 25 males) to judge 22 hypothetical dilemmas split into three different categories (non-moral, impersonal and personal moral). The TBI group presented a higher proportion of affirmative (utilitarian) responses for personal moral dilemmas when compared to controls, suggesting an atypical pattern of utilitarian judgements. We also found a negative association between the performance on recognition of social emotions and the proportion of affirmative responses on personal moral dilemmas. These results suggested that the preference for utilitarian responses in this type of dilemmas is accompanied by difficulties in social emotion recognition. Overall, our findings suggest that deontological moral judgments are associated with normal social emotion processing and that frontal lobe plays an important role in both emotion and moral judgment

Effects of grape seed extract on properties of type I collagen scaffolds

 To obtain a material with potential for use in tissue engineering, anionic collagen was obtained from porcine serosa (S) and bovine tendon (T) by alkaline hydrolysis for 72h. Part of this collagen was mixed with water to obtain 4 % (weight/weight) collagen suspension and part was solubilized in acetic acid pH 3.5 to obtain 1.5% (w/w) gel. The suspensions were mixed with their respective gels (2:1) (suspension: gel) and grape seed extract, whose main product is proanthocyanidin, was added at concentrations of 0.03% and 0.5%, thus obtaining the scaffolds SC (serosa collagen suspension and gel), TC (tendon collagen suspension and gel), SCP003 (SC with 0.03% extract), TCP003 (TC with 0.03% extract), SCP05 (SC with 0.5% extract added) and TCP05 (TC with 0.5% extract). The materials were analyzed by differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and characterized by phosphate buffered saline absorption assay and in vitro biological stability assay. By DSC it is observed that the addition of 0.5% of extract increases the denaturation temperature (Td) of collagen, indicating that at this concentration the extract acts as polymer crosslinking agent. SEM shows disorganized cross-section pores in all scaffolds, not exceeding 130 ?m. Absorption and degradation assays indicated that the addition of 0.5% extract increases the absorption of phosphate buffered saline (PBS) by the scaffolds and decreases the degradation percentage by collagenase. These results suggests that the scaffolds can be used for different applications, e.g. as hemostatic agent.