The release call as a diagnostic character between cryptic related species Odontophrynus cordobae and O. americanus (Anura: Cycloramphidae)

Diploid Odontophrynus cordobae and tetraploid O. americanus are morphologically cryptic species (Martino and Sinsch 2002). These species occurs in sympatry and syntopy in the SW of the Córdoba province, Argentina (Grenat et al. 2009). At present, individuals of O. cordobae and O. americanus can be only differentiated by mean cytogenetics (Salas 2006; Rosset et al. 2006), which involves the sacrifice of the animal, and erythrometry (Grenat et al. 2009). We propose the release call as a novel character to differentiate these species. Release vocalizations are produced by male anurans as a negative response to male mating attempts. Some studies demonstrated that the release calls of several anuran species differed specifically and that temporal structure of calls could be phylogenetically informative (Brown and Littlejohn 1972; Sullivan and Lamb 1988; Sullivan and Malmos 1994; di Tada et al. 2001). In the genus Odontophrynus, only the release call of O. cordobae has been described (Grenat et al. 2012). The sonograms of O. americanus and O. occidentalis release calls were showed by Barrio (1964), but call measurements were not reported. The aims of present study are: 1) to describe the release call of Odontophrynus americanus; 2) to compare release calls of cryptic species O. cordobae and O. americanus; 3) to evaluate release calls as diagnostic character to distinguish between these species.Fil: Grenat, Pablo Raúl. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentin

Cryptic Triploids and Leaky Premating Isolation in an Odontophrynus Hybrid Zone

The diploid Odontophrynus cordobae and its autopolyploid counterpart O. americanus (4n) co-occur in a small-sized contact zone in Central Argentina, together with numerous specimens of a cryptic triploid taxon. Additionally, we monitor another five localities inhabited by taxa of this species complex within a radius of 76 km north and south of the contact zone to look for possible co-occurrence of taxa. In this study, we analyze the reproductive interactions in this breeding assemblage covering three levels of ploidy. We focus on the advertisement call structure as a powerful mechanism of premating isolation and on the mating preferences of males and females in the natural habitat. Advertisement calls of triploids were indistinguishable from those of tetraploids, and both differed significantly in pulse rate from diploids. Analyses of 21 amplexi demonstrated that heterospecific matings dominated breeding: one between a diploid female and tetraploid male, and ten between triploids and tetraploids. At three localities with syntopic diploids and tetraploids, premating isolation was almost perfect, preventing the formation of triploids. Therefore, we question the putative origin of triploids from hybridization and discuss alternatives. This unique system, including three bisexual taxa of distinct ploidy, which interact reproductively, remains to be investigated in more detail to fully understand the mechanism stabilizing its persistence.Fil: Martino, Adolfo Ludovico. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente.; Argentina. Universitat Koblenz; AlemaniaFil: Grenat, Pablo Raúl. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente.; ArgentinaFil: Sinsch, Ulrich. Universitat Koblenz; Alemani

Effect to age on the geographic variation in morphometric traits among populations of Boana cordobae (Anura: Hylidae)

La variación morfométrica del tamaño corporal es un aspecto importante de la historia natural de las especies, que ha sido de particular interés. En este estudio se estimaron diferencias en el tamaño corporal y la estructura de edad de seis poblaciones de Boana cordobae que viven a diferentes altitudes, 808-2 310 m.a.s.l. en las provincias de Córdoba y San Luis (Argentina). La técnica de esqueletocronología se utilizó para la determinación de la edad, mediante la medición de 15 variables morfométricas en 79 individuos. Las variables morfométricas mostraron diferencias significativas entre sexos, siendo las hembras de mayor tamaño que los machos, incluso cuando el efecto de la edad se tuvo en cuenta; y se encontró una relación significativa entre la edad y la mayoría de las variables morfométricas. Cuando el efecto de la edad fue removido, se encontraron diferencias significativas en el tamaño del cuerpo entre las poblaciones estudiadas. Machos de poblaciones de mayor altitud son más grandes que los de altitudes menores. Estos resultados sugieren que las diferencias en la estructura de edad entre las poblaciones es un factor de importancia a tener en cuenta para analizar la variación en el tamaño corporal de esta especie según el área geográfica.The morphometric variation of body size is an important topic of the natural history of the species which has been received particular interest. In this study, we estimated differences in body size and age structure of six populations of Boana cordobae living at different altitudes, 808-2 310 m.a.s.l. in Córdoba and San Luis provinces (Argentina). We measured 15 morphometric variables and used skeletochronology to age determina- tion of 79 individuals. Morphometric variables showed significant differences between sexes, being females larger than males, even when the effect of age was taken into account. We found a significant relationship between age and most of the morphometric variables. When removing the effects of age, we found significant inter-population differences in body size. Males from the high-elevation populations were larger than individuals from low-elevation populations. These results suggest that a difference in age structure between populations is a main factor for the geographic variation in body size of this species.Fil: Baraquet, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; ArgentinaFil: Otero, Manuel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; ArgentinaFil: Grenat, Pablo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; ArgentinaFil: Babini, María Selene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; ArgentinaFil: Martino, Adolfo Ludovico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentin

Meiotic behavior of two polyploid species of genus Pleurodema (Anura: Leiuperidae) from central Argentina

Polyploidy is an important evolutionary force but rare in vertebrates. However, in anurans, the genus Pleurodema has polyploid species, two of them tetraploid and one octoploid. The manner in which the chromosomes join in diakinesis can vary among species and, crucially, if they differ in their ploidy levels. In this work, we describe the meiotic configurations in two cryptic species from central Argentina, with different ploidy levels, Pleurodema kriegi (tetraploid) and P. cordobae (octoploid). A total of 306 diakineses from 19 individuals were analyzed. In meiosis, P. kriegi form 22 bivalents, whereas P. cordobae exhibits variation in meiotic figures. We discuss the possible allo- and autopolyploid origin of these species, and we consider that the autopolyploid origin of P. cordobae from P. kriegi might be the most feasible.Fil: Salas, Nancy Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; ArgentinaFil: Valetti, Julian Alonso. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grenat, Pablo Raúl. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Otero, Manuel Alejandro. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

A redescription of the chigger Hannemania achalai Alzuet and Mauri, 1987 (Acariformes: Prostigmata: Leeuwenhoekiidae) in frogs from Sierra Grande, Cordoba, Argentina

We redescribe the endoparasitic chigger Hannemania achalai based on specimens collected from the type frog host (Pleurodema kriegi) and an additional host (P. cordobae) in the type locality of this mite (Pampa de Achala, Córdoba province, Argentina). Hannemania achalai is diagnosed by the following characters: palpal setal formula B/B/BBB/5B, palpal claw trifurcate, galeal seta B, parasubterminala I N, 2-4 genualae I, 1 genuala II–III, femorala III absent and coxa III with 1B. This redescription is accompanied by micrographs with scanning electronic microscopy and drawings.Fil: Paredes León, Ricardo. Universidad Nacional Autónoma de México; MéxicoFil: Biolé, Fernanda Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Valetti, Julian Alonso. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Ciencias Naturales; Argentin

Integrative taxonomic reassessment of Odontophrynus populations in Argentina and phylogenetic relationships within Odontophrynidae (Anura)

Amphibians are the most vulnerable vertebrates to biodiversity loss mediated by habitat destruction, climate change and diseases. Informed conservation management requires improving the taxonomy of anurans to assess reliably the species’ geographic range. The genus Odontophrynus that is geographically refined to Argentina, Bolivia, Brazil, Uruguay and Paraguay includes currently 12 nominal species with many populations of uncertain taxonomic assignment and subsequently unclear geographic ranges. In this study, we applied integrative taxonomic methods combining molecular (mitochondrial 16S gene), allozyme, morphological and bioacoustic data to delimit species of the genus Odontophrynus sampled from throughout Argentina where most species occur. The combined evidence demonstrates one case of cryptic diversity and another of overestimation of species richness. The populations referred to as O. americanus comprise at least three species. In contrast, O. achalensis and O. barrioi represent junior synonyms of the phenotypically plastic species O. occidentalis. We conclude that each of the four species occurring in Argentina inhabits medium to large areas. The Red List classification is currently “Least Concern”. We also propose a phylogenetic hypothesis for the genus and associated genera Macrogenioglottus and Proceratophrys (Odontophrynidae)

The tadpole of Pleurodema cordobae Valetti, Salas & Martino, 2009 (Anura: Leiuperidae) from Córdoba, Argentina

The frog genus Pleurodema Tschudi, 1838 is distributed from Panama throughout South America to southern Chile and Argentina and currently is represented by 15 species, of which 10 have been recorded in Argentina (Faivovich et al. 2012). Recently, Valetti et al. (2009) re-described the tetraploid species P. kriegi and described a related cryptic species, the octoploid Pleurodema cordobae. These species are endemic of the Sierra Grande of Córdoba (Ferraro & Casagranda 2009; Valetti et al. 2009; Valetti et al. 2011) and together with the tetraploid P. bibrioni comprise a clade of polyploid cryptic species within the genus (Faivovich et al. 2012). The external morphology has been described for the tadpoles of P. bibrioni and P. kriegi by Kolenc et al. (2009), but the tadpole of P. cordobae remains undescribed. The aim of this work is to describe the larval external morphology and oral disc of new species P. cordobae.Fil: Otero, Manuel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grenat, Pablo Raúl. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valetti, Julian Alonso. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salas, Nancy Edith. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquimicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Reproductive endpoints of Rhinella arenarum (Anura, Bufonidae): Populations that persist in agroecosystems and their use for the environmental health assessment

Degradation of the environment by agriculture affects the persistence and health of the amphibian populations. Characteristics related to reproduction of anuran can be used to evaluate the status of populations and as endpoints in environmental health assessment. In this in situ study the aspects related to the amplexus and ovipositions of the bioindicator species Rhinella arenarum that inhabits agroecosystems were analyzed. The hypothesis of this study is that perturbations of agroecosystems have a negative impact on the size of reproductive adults, on the size of ovipositions and eggs, and on the survival of eggs and embryos. Study area is located in the rural landscape of central Argentina. Four sampling sites were selected: C1, C2 and C3 are ponds on agroecosystems; and SM is a reference site that is not affected by agriculture or livestock. Abundance of amplexus pairs, oviposition and tadpoles per site was recorded. Individuals´ snout–vent length (SVL) in amplexus was measured. The fecundity was calculated like number of eggs per oviposition. The eggs’ Gosner stage, the diameter eggs and the frequency of dead and abnormal eggs were recorded by oviposition. Killing-power between egg-embryo and egg-tadpole was calculated. The higher phosphate concentration was detected in all agroecosystems and nitrate was detected in C1 and C2. Conductivity, salinity and SDT were higher in C1 site Male SVL from the SM site was lower than the other sites while the largest SVL was of female from the C3 site. The higher frequencies of sprouted eggs and of dead eggs were recorded in the C2 site. Egg diameter was associated with SM and correlated negatively to SVL of the male and female. No correlation between female SVL and oviposition size was recorded. Killing-power in the passage from egg to tadpole classes was higher in the three agroecosystems. The hypothesis of this study was corroborated in part. Reproductive adults in agroecosystems did not have smaller body size. However, in the agroecosystem ponds, the eggs with smaller diameter were registered, the oviposition had higher frequency of abnormal eggs and the higher mortality was registered. This confirms the high sensitivity of the early stages to environmental disturbances and sustains their use as endpoints for the environmental health assessment.Fil: Babini, María Selene. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bionda, Clarisa de Lourdes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Salinas, Zulma Anahí. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Salas, Nancy Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentin

Anuran tadpoles inhabiting a fluoride-rich stream: diets and morphological indicators

We in situ assessed the influence of natural fluoride concentrations in lotic freshwater ecosystems on diet and morphology of Boana cordobae tadpoles. Two streams were sampled in Argentina: Los Vallecitos stream (LF-LV) and Los Cerros Negros stream (HF-CN) with low and high fluoride levels, respectively. We captured tadpoles of B. cordobae in each stream using nets. Body weight (BW), total length (TL) and body condition (BC) of tadpoles was registered. Food items were identified to genus level and assigned to functional traits. Tadpoles showed significant differences in TL between streams, with smaller individuals in HF-CN, while did not show differences in BW and BC. The diet of tadpoles consisted mostly of microalgae. In tadpoles from HF-CN stream the proportion of cyanobacteria was lower than tadpoles from LF-LV. In relation to functional traits, small algae, high profile and colonial algae were more abundant in HF-CN. Algae attached by pads showed a higher proportion in HF-CN diets and stalked algae were more abundant in LF-LV. The differences in TL and diet of tadpoles can be attributed to differences in algal community composition, with genera that are affected by high concentrations of natural fluoride; for example cyanobacteria. The low algal richness registered in HF-CN stream does not affect the physiological state of the tadpoles, possibly because of a higher algal density in HF-CN diets. However, in further studies it would be important to evaluate the population status of B. cordobae from the HF-CN, because a small body length of tadpoles could have consequences at the population level.Fil: Pollo, Favio Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente; ArgentinaFil: Cibils Martina, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente; ArgentinaFil: Otero, Manuel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente; ArgentinaFil: Baraquet, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente; ArgentinaFil: Grenat, Pablo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente; ArgentinaFil: Salas, Nancy Edith. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; ArgentinaFil: Martino, Adolfo Ludovico. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Ciencias Naturales. Cátedra de Ecología; Argentin

Efeito da temperatura sobre o canto de advertência de Pleurodema tucumanum (Anura: Leiuperidae)

El canto de advertencia es un carácter importante que permite discriminar especies,principalmente entre especies simpátricas. El género Pleurodema poseen especies crípticas y por lotanto, sus características acústicas podrían representar una herramienta útil para resolver problemastaxonómicos. Es esperado que las propiedades acústicas del canto de advertencia de los anfibiosanuros varíen con la temperatura debido a su condición ectotérmica. Nosotros evaluamos el efecto dela temperatura en las variables acústicas del canto de advertencia de Pleurodema tucumanum. Lasvariables intervalo entre cantos, número de pulsos por canto, intervalo entre pulsos y tasa de pulsosmostraron covariación con la temperatura, mientras que las variables duración del canto, duración delpulso y frecuencia dominante resultaron temperatura-independientes. El canto de advertencia de P. tucumanum corresponde a un canto simple (tren de pulsos periódico simple). El canto tuvo unaduración promedio de 289 ms, una frecuencia dominante de 2552 Hz y 35 pulsos de 2.38 ms a 22˚C.El número de pulsos incrementó junto con la temperatura. La discriminación de la variabilidadexógena a los componentes acústicos permite analizar la variabilidad intrínseca de los cantos deadvertencia.O canto de advertência representa um caráter importante para discriminar espécies,principalmente entre espécies aparentadas. O gênero Pleurodema possui espécies crípticas, portantosuas características acústicas poderiam representar uma ferramenta útil para resolver problemastaxonômicos. É esperado que as propriedades acústicas do canto de advertência dos anfíbios anurosvariem com a temperatura, devido à sua ectotermia. Avaliamos o efeito da temperatura sobre asvariáveis acústicas do canto de advertência de Pleurodema tucumanum. O intervalo entre cantos, onúmero de pulsos por canto, o intervalo entre pulsos e a taxa de pulsos mostraram covariação com atemperatura, enquanto a duração do canto, a duração do pulso e a frequência dominante foramindependentes da temperatura. O canto de advertência de P. tucumanum corresponde a uma sequênciade pulsos periódicos. O canto teve uma duração média de 289 ms, frequência dominante de 2552 Hze 35 pulsos de 2.38 ms a 22oC. O número de impulsos aumentou com a temperatura. A discriminaçãoda variabilidade exógena a partir de componentes acústicos permite analisar a variabilidade intrínsecados cantos de advertência.The advertisement call represents an important character to discriminatespecies, mainly between sibling species. The genus Pleurodema has cryptic species,therefore its acoustical characteristics could represent an useful tool to solve taxonomicproblems. It is expected that the advertisement call acoustic properties of the amphibiananurans will vary with the temperature because the frogs are ectotherms. We evaluated theeffect of temperature in the acoustic variables of the advertisement call of Pleurodematucumanum. The inter-call interval, number of pulses per call, inter-pulse interval andpulse rate showed covariation with temperature, whereas the call duration, pulse durationand dominant frequency were temperature-independent. The advertisement call of P.tucumanum corresponds to a single periodic pulse train. The call showed an averageduration of 289 ms, dominant frequency of 2552 Hz and 35 pulses of 2.38 ms at 22˚C. Thenumber of pulses increased with temperature. The discrimination of exogenous variabilityfrom the acoustic components allows one to analyze the intrinsic variability of theadvertisement calls