Ride the Wave (The Wave of Recovery): Leisure Time Activities

Ride the Wave (The Wave of Recovery) Leisure Time Activities is the title of a leisure time activities booklet that was created by Donnie Martin in partnership with Recovery in Motion (RIM, non-profit organization), as a resource to help reduce occurrences of addiction relapses in the Twin Falls community. The hope is that more recovering addicts will be successful in their recovery and transition into a recovery lifestyle becoming productive members of the Twin Falls community. The leisure time activities booklet is being distributed and edified to recovering addicts in recovery support groups by RIM support group facilitators and by the recovery group, Ride the Wave

Biolayer Interferometry: Protein-RNA Interactions.

RNA-binding proteins often contain multiple RNA-binding domains connected by short flexible linkers. This domain arrangement allows the protein to bind the RNA with greater affinity and specificity than would be possible with individual domains and sometimes to remodel its structure. It is therefore important to understand how multiple modules interact with RNA because it is the modular nature of these proteins which specifies their biological function. This chapter is concerned with the use of biolayer interferometry to study protein-RNA interactions

An optimized strategy to measure protein stability highlights differences between cold and hot unfolded states

Macromolecular crowding ought to stabilize folded forms of proteins, through an excluded volume effect. This explanation has been questioned and observed effects attributed to weak interactions with other cell components. Here we show conclusively that protein stability is affected by volume exclusion and that the effect is more pronounced when the crowder's size is closer to that of the protein under study. Accurate evaluation of the volume exclusion effect is made possible by the choice of yeast frataxin, a protein that undergoes cold denaturation above zero degrees, because the unfolded form at low temperature is more expanded than the corresponding one at high temperature. To achieve optimum sensitivity to changes in stability we introduce an empirical parameter derived from the stability curve. The large effect of PEG 20 on cold denaturation can be explained by a change in water activity, according to Privalov's interpretation of cold denaturation

Associations between inattention and impulsivity ADHD symptoms and disordered eating risk in a community sample of young adults.

BACKGROUND: Symptoms of attention deficit hyperactivity disorder (ADHD) and trait impulsivity have been associated with disordered eating but are seldom assessed in community studies, or longitudinally and little is known about the mediating mechanisms. METHODS: We tested associations between ADHD symptoms and disordered eating cross-sectionally and between trait impulsivity and disordered eating longitudinally. We utilised data from a normative cohort of young adults (642 participants: 65% female, Mage = 23 years). Participants were classified as high risk or low risk for disordered eating using the SCOFF instrument. In the first two steps of both cross-sectional and longitudinal hierarchical logistic regression models, demographics and covariates were entered. For the cross-sectional regression, Adult ADHD self-report scale (ASRS) scores, separated into inattentive and hyperactive/impulsive symptoms, were entered in the third step. In a separate longitudinal model, Barratt impulsivity scale subscales (attentional, motor and non-planning impulsivity) were entered in the third step. Depression, as assessed by the moods and feelings questionnaire (MFQ), was examined as a mediator. RESULTS: Cross-sectionally, sex, MFQ score and inattentive symptoms predicted disordered eating risk (model R2 = 20%). Longitudinally, sex, MFQ score and attentional impulsivity predicted disordered eating risk (model R2 = 16%). The relationship between inattentive symptoms and the disordered eating risk was partially mediated by MFQ score, whereas the relationship between attentional impulsivity and the disordered eating risk was fully mediated by MFQ scores. CONCLUSIONS: These data highlight (1) a specific role for inattentive symptoms of ADHD and (2) the importance of both depression and impulsivity in predicting eating disorder risk

Molecular mechanism of influenza A NS1-mediated TRIM25 recognition and inhibition

RIG-I is a viral RNA sensor that induces the production of type I interferon (IFN) in response to infection with a variety of viruses. Modification of RIG-I with K63-linked poly-ubiquitin chains, synthesised by TRIM25, is crucial for activation of the RIG-I/MAVS signalling pathway. TRIM25 activity is targeted by influenza A virus non-structural protein 1 (NS1) to suppress IFN production and prevent an efficient host immune response. Here we present structures of the human TRIM25 coiled-coil-PRYSPRY module and of complexes between the TRIM25 coiled-coil domain and NS1. These structures show that binding of NS1 interferes with the correct positioning of the PRYSPRY domain of TRIM25 required for substrate ubiquitination and provide a mechanistic explanation for how NS1 suppresses RIG-I ubiquitination and hence downstream signalling. In contrast, the formation of unanchored K63-linked poly-ubiquitin chains is unchanged by NS1 binding, indicating that RING dimerisation of TRIM25 is not affected by NS1

The Impact of the Structural Parameters of Age and Race Upon Residents\u27 Evaluations of Community.

The research reported in this dissertation deals directly with an individual\u27s perception of his or her community and family circumstances. The major question explored deals with what structural factors influence individuals\u27 perceptions. In order to examine this question, the study primarily employs structural parameters such as age, sex, race, community, etc. to note differences or similarities among subpopulations. Specifically, the study explores the differences or similarities between adults and adolescents in rural communities. The data includes both an adult and adolescent sample taken in three rural communities in South Louisiana. The racial composition of the communities includes black, Indian, and white. The findings of the study suggest a different strategy for the derivation of the community evaluation scale. Instead of developing a scale from all samples combined, factor analysis procedures of subpopulations were employed to delineate a scale of greatest convergence for all respondents and subpopulations. This offers support to the contention that it is important to recognize potential subgroups within community settings whose perceptions may differ significantly from the total sample. Other findings indicate that age and race have influences upon community and family evaluations, particularly when subpopulations such as parent vs. child, racial categories, etc. are explored. Whites tend to rate aspects of community and family circumstances higher than do nonwhites, whereas adolescent offspring have a more positive view of family circumstances than do their parents, especially among nonwhites. Surprisingly, community residence has no impact upon community evaluation or family satisfaction. House condition and sex have no influence upon assessments of community, but both influence family satisfaction. The better the house condition among adults, the higher the score for family circumstances, whereas females tend to rate their family situation higher than males among the adolescents. The major implication of this study is that structural parameters like age and race are factors along which perceptions of community become organized. This is supported empirically in this study. This substantiates the importance of structural parameters in underlying the differentiation among groups, and their potential for governing social interaction. In addition, this study is a supportive case for continued examination and research into subjective indicators of social reality. The present study should be considered as part of a continuing development of community satisfaction research in rural communities. It is fairly evident from this study that the determination of community evaluative responses of residents is not easily captured. It is the task of sociologists to recognize potential subgroups within community settings whose realities may differ significantly from the representative residents. We cannot allow ourselves the luxury of making assumptions about people\u27s beliefs and perceptions without some notion of relevant structural dimensions along which realities may be experienced and constructed

The structure of the RbBP5 β-propeller domain reveals a surface with potential nucleic acid binding sites

The multi-protein complex WRAD, formed by WDR5, RbBP5, Ash2L and Dpy30, binds to the MLL SET domain to stabilize the catalytically active conformation required for histone H3K4 methylation. In addition, the WRAD complex contributes to the targeting of the activated complex to specific sites on chromatin. RbBP5 is central to MLL catalytic activation, by making critical contacts with the other members of the complex. Interestingly its only major structural domain, a canonical WD40 repeat -propeller, is not implicated in this function. Here, we present the structure of the RbBP5 -propeller domain revealing a distinct, feature rich surface, dominated by clusters of Arginine residues. Our nuclear magnetic resonance binding data supports the hypothesis that in addition to the role of RbBP5 in catalytic activation, its -propeller domain is a platform for the recruitment of the MLL complexes to chromatin targets through its direct interaction with nucleic acids

KH domains with impaired nucleic acid binding as a tool for functional analysis

In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how the different KH modules participate in the recognition of the RNA targets is necessary to dissect the way these proteins operate. We have designed a KH mutant with impaired RNA-binding capability for general use in exploring the role of individual KH domains in the combinatorial functional recognition of RNA targets. A double mutation in the hallmark GxxG loop (GxxG-to-GDDG) impairs nucleic acid binding without compromising the stability of the domain. We analysed the impact of the GDDG mutations in individual KH domains on the functional properties of KSRP as a prototype of multiple KH domain-containing proteins. We show how the GDDG mutant can be used to directly link biophysical information on the sequence specificity of the different KH domains of KSRP and their role in mRNA recognition and decay. This work defines a general molecular biology tool for the investigation of the function of individual KH domains in nucleic acid binding proteins