Retinal cone photoreceptors of the deer mouse Peromyscus maniculatus : development, topography, opsin expression and spectral tuning

A quantitative analysis of photoreceptor properties was performed in the retina of the nocturnal deer mouse, Peromyscus maniculatus, using pigmented (wildtype) and albino animals. The aim was to establish whether the deer mouse is a more suitable model species than the house mouse for photoreceptor studies, and whether oculocutaneous albinism affects its photoreceptor properties. In retinal flatmounts, cone photoreceptors were identified by opsin immunostaining, and their numbers, spectral types, and distributions across the retina were determined. Rod photoreceptors were counted using differential interference contrast microscopy. Pigmented P. maniculatus have a rod-dominated retina with rod densities of about 450.000/mm(2) and cone densities of 3000 - 6500/mm(2). Two cone opsins, shortwave sensitive (S) and middle-to-longwave sensitive (M), are present and expressed in distinct cone types. Partial sequencing of the S opsin gene strongly supports UV sensitivity of the S cone visual pigment. The S cones constitute a 5-15% minority of the cones. Different from house mouse, S and M cone distributions do not have dorsoventral gradients, and coexpression of both opsins in single cones is exceptional (<2% of the cones). In albino P. maniculatus, rod densities are reduced by approximately 40% (270.000/mm(2)). Overall, cone density and the density of cones exclusively expressing S opsin are not significantly different from pigmented P. maniculatus. However, in albino retinas S opsin is coexpressed with M opsin in 60-90% of the cones and therefore the population of cones expressing only M opsin is significantly reduced to 5-25%. In conclusion, deer mouse cone properties largely conform to the general mammalian pattern, hence the deer mouse may be better suited than the house mouse for the study of certain basic cone properties, including the effects of albinism on cone opsin expression

Unrecognized Backscattering in Low Energy Beta Spectroscopy

We present studies on electron backscattering from the surface of plastic scintillator beta detectors. By using a setup of two detectors coaxial with a strong external magnetic field - one detector serving as primary detector, the other as veto-detector to detect backscattering - we investigate amount and spectrum of unrecognized backscattering, i.e. events where only one detector recorded a trigger signal. The implications are important for low energy particle physics experiments.Comment: 5 pages, 8 figures; v2: published NIM A versio

A black-hole mass measurement from molecular gas kinematics in NGC4526

The masses of the supermassive black-holes found in galaxy bulges are correlated with a multitude of galaxy properties, leading to suggestions that galaxies and black-holes may evolve together. The number of reliably measured black-hole masses is small, and the number of methods for measuring them is limited, holding back attempts to understand this co-evolution. Directly measuring black-hole masses is currently possible with stellar kinematics (in early-type galaxies), ionised-gas kinematics (in some spiral and early-type galaxies) and in rare objects which have central maser emission. Here we report that by modelling the effect of a black-hole on the kinematics of molecular gas it is possible to fit interferometric observations of CO emission and thereby accurately estimate black hole masses. We study the dynamics of the gas in the early-type galaxy NGC4526, and obtain a best fit which requires the presence of a central dark-object of 4.5(+4.2-3.0)x10^8 Msun (3 sigma confidence limit). With next generation mm-interferometers (e.g. ALMA) these observations could be reproduced in galaxies out to 75 megaparsecs in less the 5 hours of observing time. The use of molecular gas as a kinematic tracer should thus allow one to estimate black-hole masses in hundreds of galaxies in the local universe, many more than accessible with current techniques.Comment: To appear in Nature online on 30/01/2013. 3 Pages, 2 Figures (plus two pages of supplementary information