Parental Leave, (In)formal Childcare and Long-term Child Outcomes

There is a strong debate about who should provide care to young children. Governments offer two alternative types of institutions: formal childcare and parental leave. We assess the effectiveness of these two competing institutions in promoting child development by comparing how a major parental leave extension from one to two years affected Austrian children's long-term outcomes in communities with and without formal childcare facilities for under-3-year-olds. Empirical identification of treatment effects is based on a sharp birthday cutoff-based discontinuity in the eligibility for extended parental leave and geographical variation in formal childcare. We find evidence that the counterfactual mode of care is decisive. If formal childcare is available, the reform induced a replacement of formal childcare by maternal care and had zero (or negative effects) on child outcomes. Whereas if formal childcare is not available, informal childcare was replaced by maternal care, and the reform improved child outcomes. This heterogeneity is driven by the additional time with the mother in the second year of the child's life and not by a change in maternal income. We conclude that care provided by mothers or formal institutions is superior to informal care-arrangements

Parental Leave, (In)formal Childcare and Long-term Child Outcomes

We provide a novel interpretation of the estimated treatment effects from evaluations of parental leave reforms. Accounting for the counterfactual mode of care is crucial in the analysis of child outcomes and potential mediators. We evaluate a large and generous parental leave extension in Austria exploiting a sharp birthday cutoff-based discontinuity in the eligibility for extended parental leave and geographical variation in formal childcare. We find that estimated treatment effects on long-term child outcomes differ substantially according to the availability of formal childcare and the mother’s counterfactual work behavior. We show that extending parental leave has significant positive effects on children’s health and human capital outcomes only if the reform induces a replacement of informal childcare with maternal care. We conclude that care provided by mothers (or formal institutions) is superior to informal care arrangements

Kinderbetreuung

Im Rahmen der vom Bundesministerium für Familie, Senioren, Frauen und Jugend (BMFSFJ) und vom Bundesministerium der Finanzen (BMF) in Auftrag gegebenen Gesamtevaluation von zentralen ehe- und familienbezogenen Leistungen wurden in der vorliegenden Studie die Auswirkungen öffentlich geförderter Kinderbetreuung auf Familien untersucht. In einer ex-post Analyse wurden die Effekte von Kinderbetreuung auf folgende familienpolitische Ziele identifiziert: Vereinbarkeit von Familie und Beruf/wirtschaftliche Stabilität und soziale Teilhabe von Familien, Steigerung der Geburtenrate/Realisierung von Kinderwünschen. Die Wirkungsanalysen wurden für Familien mit unter-dreijährigen Kindern (U3), mit drei- bis sechsjährigen Kindern (Ü3) sowie für Schulkinder (Ü6) separat durchgeführt. Im Anschluss wurden in Effizienzanalysen die Selbstfinanzierungsquoten des Ausbaus der Kindertagesbetreuung untersucht

A luciferase-based quick potency assay to predict chondrogenic differentiation.

Chondrogenic differentiation of adipose derived stem cells (ASC) is challenging but highly promising for cartilage repair. Large donor variability of chondrogenic differentiation potential raises the risk for transplantation of cells with reduced efficacy and a low chondrogenic potential. Therefore quick potency assays are required in order to control the potency of the isolated cells before cell transplantation. Current in vitro methods to analyze the differentiation potential are time consuming and thus, a novel enhancer and tissue-specific promoter combination was employed for the detection of chondrogenic differentiation of ASC in a novel quick potency bioassay. Human primary ASC were co-transfected with the Metridia luciferase based collagen type II reporter gene pCMVE_ACDCII-MetLuc together with a Renilla control plasmid and analyzed for their chondrogenic potential. On day 3 after chondrogenic induction, the luciferase activity was induced in all tested donors under three dimensional (3D) culture conditions and in a second approach also under 2D culture conditions. With our newly developed quick potency bioassay we can determine chondrogenic potential already after 3 days of chondrogenic induction and under 2D culture conditions. This will enhance the efficiency of testing cell functionality, which should allow in the future to predict the suitability of cells derived from individual patients for cell therapies, in a very short time and at low costs

Improving statistical inference on pathogen densities estimated by quantitative molecular methods: malaria gametocytaemia as a case study

BACKGROUND: Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to estimate pathogen density in a variety of clinical and epidemiological contexts. These methods are often classified as semi-quantitative, yet estimates of reliability or sensitivity are seldom reported. Here, a statistical framework is developed for assessing the reliability (uncertainty) of pathogen densities estimated using QMMs and the associated diagnostic sensitivity. The method is illustrated with quantification of Plasmodium falciparum gametocytaemia by QT-NASBA. RESULTS: The reliability of pathogen (e.g. gametocyte) densities, and the accompanying diagnostic sensitivity, estimated by two contrasting statistical calibration techniques, are compared; a traditional method and a mixed model Bayesian approach. The latter accounts for statistical dependence of QMM assays run under identical laboratory protocols and permits structural modelling of experimental measurements, allowing precision to vary with pathogen density. Traditional calibration cannot account for inter-assay variability arising from imperfect QMMs and generates estimates of pathogen density that have poor reliability, are variable among assays and inaccurately reflect diagnostic sensitivity. The Bayesian mixed model approach assimilates information from replica QMM assays, improving reliability and inter-assay homogeneity, providing an accurate appraisal of quantitative and diagnostic performance. CONCLUSIONS: Bayesian mixed model statistical calibration supersedes traditional techniques in the context of QMM-derived estimates of pathogen density, offering the potential to improve substantially the depth and quality of clinical and epidemiological inference for a wide variety of pathogens

28 th European Photovoltaic Solar Energy Conference, 30

ABSTRACT: As grid price rises and the feed-in tariff declines, the economics of local storage become increasingly lucrative to the system owner. The attractiveness of a local storage investment is compounded in the presence of a PV grid injection cap. The larger the PV system size is relative to this cap level, the greater the opportunity exists to charge the local storage with PV production that would otherwise be dissipated without credit. This study utilizes two household demand profiles that represent the extremes of the potential for local PV self-consumption and, consequently, the range of economic potential that exists for local storage to be coupled with residential PV systems. A series of algorithms were subsequently developed to analyze the related benefit potential of delayed storage charging to target instances of excess PV production depending upon the grid injection cap

Integrated Flow Chamber System for Live Cell Microscopy

In vitro quantification of the effect of mechanical loads on cells by live microscopy requires precise control of load and culture environment. Corresponding systems are often bulky, their setup and maintenance are time consuming, or the cell yield is low. Here, we show the design and initial testing of a new cell culture system that fits on standard light microscope stages. Based on the parallel plate principle, the system allows for live microscopy of cells exposed to flow-induced shear stress, features short setup time and requires little user interaction. An integrated feedback-controlled heater and a bubble trap enable long observation times. The key design feature is the possibility for quick exchange of the cultured cells. We present first test results that focus on verifying the robustness, biocompatibility, and ease of use of the device

Adjoint quarks and fermionic boundary conditions

We study quenched SU(2) lattice gauge theory with adjoint fermions in a wide range of temperatures. We focus on spectral quantities of the Dirac operator and use the temporal fermionic boundary conditions as a tool to probe the system. We determine the deconfinement temperature through the Polyakov loop, and the chiral symmetry restoration temperature for adjoint fermions through the gap in the Dirac spectrum. This chiral transition temperature is about four times larger than the deconfinement temperature. In between the two transitions we find that the system is characterized by a non-vanishing chiral condensate which differs for periodic and anti-periodic fermion boundary conditions. Only for the latter (physical) boundary conditions, the condensate vanishes at the chiral transition. The behavior between the two transitions suggests that deconfinement manifests itself as the onset of a dependence of spectral quantities of the Dirac operator on boundary conditions. This picture is supported further by our results for the dual chiral condensate.Comment: Minor changes; final version to appear in JHE

Afferent signalling from the acid-challenged rat stomach is inhibited and gastric acid elimination is enhanced by lafutidine

<p>Abstract</p> <p>Background</p> <p>Lafutidine is a histamine H₂receptor antagonist, the gastroprotective effect of which is related to its antisecretory activity and its ability to activate a sensory neuron-dependent mechanism of defence. The present study investigated whether intragastric administration of lafutidine (10 and 30 mg/kg) modifies vagal afferent signalling, mucosal injury, intragastric acidity and gastric emptying after gastric acid challenge.</p> <p>Methods</p> <p>Adult rats were treated with vehicle, lafutidine (10 – 30 mg/kg) or cimetidine (10 mg/kg), and 30 min later their stomachs were exposed to exogenous HCl (0.25 M). During the period of 2 h post-HCl, intragastric pH, gastric volume, gastric acidity and extent of macroscopic gastric mucosal injury were determined and the activation of neurons in the brainstem was visualized by c-Fos immunocytochemistry.</p> <p>Results</p> <p>Gastric acid challenge enhanced the expression of c-Fos in the nucleus tractus solitarii but caused only minimal damage to the gastric mucosa. Lafutidine reduced the HCl-evoked expression of c-Fos in the NTS and elevated the intragastric pH following intragastric administration of excess HCl. Further analysis showed that the gastroprotective effect of lafutidine against excess acid was delayed and went in parallel with facilitation of gastric emptying, measured indirectly via gastric volume changes, and a reduction of gastric acidity. The H₂receptor antagonist cimetidine had similar but weaker effects.</p> <p>Conclusion</p> <p>These observations indicate that lafutidine inhibits the vagal afferent signalling of a gastric acid insult, which may reflect an inhibitory action on acid-induced gastric pain. The ability of lafutidine to decrease intragastric acidity following exposure to excess HCl cannot be explained by its antisecretory activity but appears to reflect dilution and/or emptying of the acid load into the duodenum. This profile of actions emphasizes the notion that H₂receptor antagonists can protect the gastric mucosa from acid injury independently of their ability to suppress gastric acid secretion.</p