Development of space technology for ecological habitats

The development of closed ecological systems for space stations is discussed. Growth chambers, control systems, microgravity, ecosystem stability, lighting equipment, and waste processing systems are among the topics discussed

Biomedical Research Division significant accomplishments for FY 1983

Various research and technology activities of Ames Research Center's Biomedical Research Division are described. Contributions to the Space Administration's goals in the life sciences include research in operational medicine, cardiovascular deconditioning, motion sickness, bone alterations, muscle atrophy, fluid and electrolyte changes, radiation effects and protection, human behavior and performance, general biomedical research, and gravitational biology

Controlled Ecological Life Support Systems: CELSS 1985 Workshop

Various topics related to closed ecological systems are discussed. Space habitats, vegetative growth, photosynthesis, recycling, culture techniques, waste utilization bioreactors and controlled atmospheres on space stations are among the topics covered

The synergistic effect of prebiotics, probiotics and antioxidants on dogs with chronic kidney disease

The use of probiotics, prebiotics and antioxidants could be found beneficial for dogs with chronic kidney disease (CKD). The aim of our case-control study is to evaluate the synergistic effect of a diet integrated with a supplement containing probiotics (Lactobacillus acidophilus), prebiotics (fructoligosaccharides) and antioxidants (Olea Europaea extract) on the nutritional status and on serum and urinary parameters of dogs with CKD. A total of 30 dogs classified with IRIS CKD stage 3 were enrolled and randomly assigned to a control (CG, n = 15) and a treated (TG n = 15) group. The trial consisted in a 7-days adaptation period, followed by 90 days where animals in the TR group received the supplement, while in the CG group the placebo. No significant changes in body weight and body condition score were recorded. We recorded a significant improvement of the protein plasmatic level and a decrease in blood phosphorus, systolic pressure, BUN, proteinuria and urine protein-to-creatinine ratio throughout the trial in the TG compared to the CG group. Furthermore, the parameters related to inflammation and oxidative stress (C-reactive protein and Reactive Oxygen Metabolite- derived compound, respectively) were lower in the TG than in the CG group throughout the study. Our results showed that the supplement allows to maintain the correct nutritional status and to improve blood and kidney parameters in dogs with advance stage of CKD. This supplement could be considered as a new nutritional approach for treating this condition.HIGHLIGHTS Diet supplemented with prebiotics, probiotics and antioxidants is safe for dogs with CKD. The synergic effect of prebiotics, probiotics and antioxidants included in the supplement under study shows the maintenance of a good nutritional status and the improvement of blood and urinary parameters in dogs with CKD

Multi-dimensional scaling techniques unveiled gain1q&loss13q co-occurrence in Multiple Myeloma patients with specific genomic, transcriptional and adverse clinical features

The complexity of Multiple Myeloma (MM) is driven by several genomic aberrations, interacting with disease-related and/or -unrelated factors and conditioning patients’ clinical outcome. Patient’s prognosis is hardly predictable, as commonly employed MM risk models do not precisely partition high- from low-risk patients, preventing the reliable recognition of early relapsing/refractory patients. By a dimensionality reduction approach, here we dissect the genomic landscape of a large cohort of newly diagnosed MM patients, modelling all the possible interactions between any MM chromosomal alterations. We highlight the presence of a distinguished cluster of patients in the low-dimensionality space, with unfavorable clinical behavior, whose biology was driven by the co-occurrence of chromosomes 1q CN gain and 13 CN loss. Presence or absence of these alterations define MM patients overexpressing either CCND2 or CCND1, fostering the implementation of biology-based patients’ classification models to describe the different MM clinical behaviors

Integrative analysis of the genomic and transcriptomic landscape of double-refractory multiple myeloma

In multiple myeloma, novel treatments with proteasome inhibitors (PIs) and immunomodulatory agents (IMiDs) have prolonged survival but the disease remains incurable. At relapse, next-generation sequencing has shown occasional mutations of drug targets but has failed to identify unifying features that underlie chemotherapy resistance. We studied 42 patients refractory to both PIs and IMiDs. Whole-exome sequencing was performed in 40 patients, and RNA sequencing (RNA-seq) was performed in 27. We found more mutations than were reported at diagnosis and more subclonal mutations, which implies ongoing evolution of the genome of myeloma cells during treatment. The mutational landscape was different from that described in published studies on samples taken at diagnosis. The TP53 pathway was the most frequently inactivated (in 45% of patients). Conversely, point mutations of genes associated with resistance to IMiDs were rare and were always subclonal. Refractory patients were uniquely characterized by having a mutational signature linked to exposure to alkylating agents, whose role in chemotherapy resistance and disease progression remains to be elucidated. RNA-seq analysis showed that treatment or mutations had no influence on clustering, which was instead influenced by karyotypic events. We describe a cluster with both amp(1q) and del(13) characterized by CCND2 upregulation and also overexpression of MCL1, which represents a novel target for experimental treatments. Overall, high-risk features were found in 65% of patients. However, only amp(1q) predicted survival. Gene mutations of IMiD and PI targets are not a preferred mode of drug resistance in myeloma. Chemotherapy resistance of the bulk tumor population is likely attained through differential, yet converging evolution of subclones that are overall variable from patient to patient and within the same patient

Search for massive rare particles with MACRO

Massive rare particles have been searched for in the penetrating cosmic radiation using the MACRO apparatus at the Gran Sasso National Laboratories. Liquid scintillators, streamer tubes and nuclear track detectors have been used to search for magnetic monopoles (MMs). Based on no observation of such signals, stringent flux limits are established for MMs as slow as a few 10^(-5)c. The methods based on the scintillator and on the nuclear track subdetectors were also applied to search for nuclearites. Preliminary results of the searches for charged Q-balls are also presented.Comment: 20 pages, 9 EPS figures included with epsfi

Observation of the Shadowing of Cosmic Rays by the Moon using a Deep Underground Detector

Using data collected by the MACRO experiment during the years 1989-1996, we show evidence for the shadow of the moon in the underground cosmic ray flux with a significance of 3.6 sigma. This detection of the shadowing effect is the first by an underground detector. A maximum-likelihood analysis is used to determine that the angular resolution of the apparatus is 0.9+/-0.3 degrees. These results demonstrate MACRO's capabilities as a muon telescope by confirming its absolute pointing ability and quantifying its angular resolution.Comment: 14 pages, 8 figures Submitted to Phys. Rev.

Neutrino astronomy with the MACRO detector

High energy gamma ray astronomy is now a well established field and several sources have been discovered in the region from a few GeV up to several TeV. If sources involving hadronic processes exist, the production of photons would be accompanied by neutrinos too. Other possible neutrino sources could be related to the annihilation of WIMPs at the center of galaxies with black holes. We present the results of a search for point-like sources using 1100 upward-going muons produced by neutrino interactions in the rock below and inside the MACRO detector in the underground Gran Sasso Laboratory. These data show no evidence for a possible neutrino point-like source or for possible correlations between gamma ray bursts and neutrinos. They have been used to set flux upper limits for candidate point-like sources which are in the range 10^-14-10^-15 cm-2 s-1.Comment: 37 pages, 15 figures, replacement due to a typo in tab. 6, AASLaTex, submitted to Ap

Search for Nucleon Decays induced by GUT Magnetic Monopoles with the MACRO Experiment

The interaction of a Grand Unification Magnetic Monopole with a nucleon can lead to a barion-number violating process in which the nucleon decays into a lepton and one or more mesons (catalysis of nucleon decay). In this paper we report an experimental study of the effects of a catalysis process in the MACRO detector. Using a dedicated analysis we obtain new magnetic monopole (MM) flux upper limits at the level of $\sim 3\cdot 10^{-16} cm^{-2} s^{-1} sr^{-1}$ for $1.1\cdot 10^{-4} \le |\beta| \le 5\cdot 10^{-3}$, based on the search for catalysis events in the MACRO data. We also analyze the dependence of the MM flux limit on the catalysis cross section.Comment: 12 pages, Latex, 10 figures and 2 Table