Use of NWCSAF NWC/GEO Software Package with MSG, HIMAWARI-8/9 and GOES-13/16 Satellites

Ponencias presentadas en: Joint EUMETSAT/AMS/NOAA Conference 2019 celebrada en Boston del 30 de septiembre al 4 de octubre de 2019

The nowcasting SAF products and services: recent improvements in the new SW packages PPS v2018 and GEO v2018 and future plans

Presentación realizada en la 3rd European Nowcasting Conference, celebrada en la sede central de AEMET en Madrid del 24 al 26 de abril de 2019

Doñana. Acta vertebrata. vol 23 (1)

Variaciones geográfica y temporal en la dieta de la lechuza campestre (Asio flammeus) en EuropaVariaciones estacionales en la dieta de la lechuza común (Tyto alba) en dos nidos de diferente localizaciónDinámica anual de la comunidad de paseriformes en carrizales costeros del sudeste ibéricoSedentarismo, áreas de campeo y selección de microhábitats en el lagarto verdinegro Lacerta schreiberEstudio morfométrico y del crecimiento de Apodemus sylvaticus L., 1758 (Rodentia, Muridae) en el norte de la Península IbéricaLas Gaviotas patiamarillas (Larus cachinnans) de la colonia de las Islas Medes (NE de España) como posibles agentes transmisores de contaminación microbiana.Dieta de la garduña (Martes foina Erxlebeb, 1777) en una localidad de las sierras subbéticas de Granada (sureste de España)Situación del lince ibérico en la Sierra de GataNotas sobre la reproducción de una población semicautiva de tortuga mediterránea Testudo hermanni en Valencia (E, España)Peer reviewe

Quid: observatorio de medios

El informe está dividido en cuatro apartados: “Derecho a la información y transparencia”, “La televisión mexicana”, “Empresas y prácticas periodísticas” y “Los que se fueron”. En el primero de ellos se presenta un texto que ayuda a entender cuál es el momento en el que se encuentran las propuestas legislativas para regular a los medios y las telecomunicaciones en México, y una evaluación de los primeros cinco años del Instituto de Transparencia e Información Pública de Jalisco. El segundo apartado del informe es ecléctico, pues se compone de artículos que trabajan distintas temáticas de la televisión:la estructura y oferta de la televisión en nuestro país (en particular en la ciudad de Guadalajara), la televisión por cable (enfatizando el caso de Megacable), un recuento de cómo se gestó el Canal 44 y de sus prospectivas en 2011, y los mundiales de futbol. La tercera parte del informe documenta algunas de las situaciones más importantes que se viven en el periodismo local: estos trabajos presentan sistemas en crisis (alta vulnerabilidad de los periodistas mexicanos ante un clima de violencia que lejos de disminuir va en aumento, y la participación, por acción u omisión, del Estado mexicano en la sistemática violación de los derechos de quienes dedican su vida al trabajo periodístico. Los siguientes artículos tratan sobre las transformaciones de las empresas periodísticas, particularmente las del sector de la prensa escrita: la rápida e inexorable desaparición de los suplementos culturales, y una radiografía sobre las formas de producción de algunas secciones internacionales de los periódicos tapatíos. Al final se presentan las semblanzas de José Galindo, Raúl Mora Lomelí, S.J., Tomás Eloy Martínez y Juan Pablo Rosell.ITESO, A.C

A New Crocodylian from the Late Maastrichtian of Spain: Implications for the Initial Radiation of Crocodyloids

The earliest crocodylians are known primarily from the Late Cretaceous of North America and Europe. The representatives of Gavialoidea and Alligatoroidea are known in the Late Cretaceous of both continents, yet the biogeographic origins of Crocodyloidea are poorly understood. Up to now, only one representative of this clade has been known from the Late Cretaceous, the basal crocodyloid Prodiplocynodon from the Maastrichtian of North America.The fossil studied is a skull collected from sandstones in the lower part of the Tremp Formation, in Chron C30n, dated at -67.6 to 65.5 Ma (late Maastrichtian), in Arén (Huesca, Spain). It is located in a continuous section that contains the K/P boundary, in which the dinosaur faunas closest to the K/P boundary in Europe have been described, including Arenysaurus ardevoli and Blasisaurus canudoi. Phylogenetic analysis places the new taxon, Arenysuchus gascabadiolorum, at the base of Crocodyloidea.The new taxon is the oldest crocodyloid representative in Eurasia. Crocodyloidea had previously only been known from the Palaeogene onwards in this part of Laurasia. Phylogenetically, Arenysuchus gascabadiolorum is situated at the base of the first radiation of crocodyloids that occurred in the late Maastrichtian, shedding light on this part of the cladogram. The presence of basal crocodyloids at the end of the Cretaceous both in North America and Europe provides new evidence of the faunal exchange via the Thulean Land Bridge during the Maastrichtian

Role of age and comorbidities in mortality of patients with infective endocarditis

[Purpose]: The aim of this study was to analyse the characteristics of patients with IE in three groups of age and to assess the ability of age and the Charlson Comorbidity Index (CCI) to predict mortality. [Methods]: Prospective cohort study of all patients with IE included in the GAMES Spanish database between 2008 and 2015.Patients were stratified into three age groups:<65 years,65 to 80 years,and ≥ 80 years.The area under the receiver-operating characteristic (AUROC) curve was calculated to quantify the diagnostic accuracy of the CCI to predict mortality risk. [Results]: A total of 3120 patients with IE (1327 < 65 years;1291 65-80 years;502 ≥ 80 years) were enrolled.Fever and heart failure were the most common presentations of IE, with no differences among age groups.Patients ≥80 years who underwent surgery were significantly lower compared with other age groups (14.3%,65 years; 20.5%,65-79 years; 31.3%,≥80 years). In-hospital mortality was lower in the <65-year group (20.3%,<65 years;30.1%,65-79 years;34.7%,≥80 years;p < 0.001) as well as 1-year mortality (3.2%, <65 years; 5.5%, 65-80 years;7.6%,≥80 years; p = 0.003).Independent predictors of mortality were age ≥ 80 years (hazard ratio [HR]:2.78;95% confidence interval [CI]:2.32–3.34), CCI ≥ 3 (HR:1.62; 95% CI:1.39–1.88),and non-performed surgery (HR:1.64;95% CI:11.16–1.58).When the three age groups were compared,the AUROC curve for CCI was significantly larger for patients aged <65 years(p < 0.001) for both in-hospital and 1-year mortality. [Conclusion]: There were no differences in the clinical presentation of IE between the groups. Age ≥ 80 years, high comorbidity (measured by CCI),and non-performance of surgery were independent predictors of mortality in patients with IE.CCI could help to identify those patients with IE and surgical indication who present a lower risk of in-hospital and 1-year mortality after surgery, especially in the <65-year group

Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence.Integrin α6β4 is a major component of hemidesmosomes that mediate the stable anchorage of epithelial cells to the underlying basement membrane. Integrin α6β4 has also been implicated in cell proliferation and migration and in carcinoma progression. The third and fourth fibronectin type III domains (FnIII-3,4) of integrin β4 mediate binding to the hemidesmosomal proteins BPAG1e and BPAG2, and participate in signalling. Here, it is demonstrated that X-ray crystallography, small-angle X-ray scattering and double electron-electron resonance (DEER) complement each other to solve the structure of the FnIII-3,4 region. The crystal structures of the individual FnIII-3 and FnIII-4 domains were solved and the relative arrangement of the FnIII domains was elucidated by combining DEER with site-directed spin labelling. Multiple structures of the interdomain linker were modelled by Monte Carlo methods complying with DEER constraints, and the final structures were selected against experimental scattering data. FnIII-3,4 has a compact and cambered flat structure with an evolutionary conserved surface that is likely to correspond to a protein-interaction site. Finally, this hybrid method is of general application for the study of other macromolecules and complexes.This work was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund grants BFU2009-08389 and BFU2012-32847 (to JMdP), by SNF grant 200020_14441 (to GJ) and by the Netherlands Science Foundation and the Dutch Cancer Society (to AS). NAG received a JAE contract (CSIC), RMB received Juan de la Cierva and Ramon y Cajal contracts and JAM received a fellowship from the University of Salamanca. This research received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under BioStruct-X (grant agreement No. 283570).Peer Reviewe

Structure of dimeric fragments of the plakin domain

Resumen del póster presentado al XIVth Congress of the Spanish Biophysical Society, celebrado en Alcalá de Henares (Madrid-España) del 11 al 13 de junio de 2014.The plakin family consists of very large proteins involved in the interconnection of multiple systems of the cytoskeleton and their connection to adhesion complexes. In mammals the plakin family includes plectin, desmoplakin, the bullous pemphigoid antigen 1, the microtubule actin crosslinking factor 1, envoplakin, periplakin, and epiplakin. Plakins have a tripartite modular structure. The N-terminal region contains a conserved segment named the plakin domain (~1000 residues) that consists of up to nine spectrin repeats (SR1-SR9) and an SH3 domain; the plakin domains mediate the interaction with proteins in adhesion complexes. Downstream of the plakin domain extends a central rod domain (900 to 1300 residues) that mediates homo-dimerization via coiled-coil interactions. Finally, the C-terminal region contains binding sites for intermediate filaments. We have recently solved the crystal structure of a 39-residues fragment of the N-terminal region of the rod domain of plectin, which adopts a non-physiological antiparallel coiled-coil in the crystal, suggesting that the formation of parallel dimers requires larger segments of the rod domain. Here, we have created dimeric fragments of the plakin domain of plectin and desmoplakin by exchanging the large rod domain for the dimeric coiled-coil of GCN4. These fragments were analyzed by small angle x-ray scattering (SAXS) that confirmed the dimeric oligomerization state. Analysis of the SAXS data indicates that the inter-protomer contacts extend along the C-terminal segment of the plakin domain, but not in the N-terminal half of the plakin domain. These results have implications for the mechanical properties of plakins and their interaction with other proteins in adhesion complexes.Peer Reviewe

The structure of the plakin domain of plectin reveals an extended rod-like shape

Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. Most plakins contain a plakin domain formed by up to nine spectrin repeats (SR1-SR9) and an SH3 domain. The plakin domains of plectin and other plakins harbor binding sites for junctional proteins. We have combined x-ray crystallography with small angle x-ray scattering (SAXS) to elucidate the structure of the plakin domain of plectin, extending our previous analysis of the SR1 to SR5 region. Two crystal structures of the SR5-SR6 region allowed us to characterize its uniquely wide inter-repeat conformational variability. We also report the crystal structures of the SR7-SR8 region, refined to 1.8 Å, and the SR7-SR9 at lower resolution. The SR7-SR9 region, which is conserved in all other plakin domains, forms a rigid segment stabilized by uniquely extensive inter-repeat contacts mediated by unusually long helices in SR8 and SR9. Using SAXS we show that in solution the SR3-SR6 and SR7-SR9 regions are rod-like segments and that SR3-SR9 of plectin has an extended shape with a small central kink. Other plakins, such as bullous pemphigoid antigen 1 and microtubule and actin cross-linking factor 1, are likely to have similar extended plakin domains. In contrast, desmoplakin has a two-segment structure with a central flexible hinge. The continuous versus segmented structures of the plakin domains of plectin and desmoplakin give insight into how different plakins might respond to tension and transmit mechanical signals.This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund, Grants BFU2012-32847 and BFU2015-69499-P (to J. M. dP), as well as by the Netherlands Science Foundation and the Dutch Cancer Society (to A. S.). This work also was supported by the European Community's Seventh Framework Programme (FP7/2007–2013) under BioStruct-X Grant Agreement 283570.Peer Reviewe

Structure of the plakin domain of plectin: implications for the mechanical properties of plakins

Resumen del póster presentado a la CNIC Conference: Mechanical forces in physiology and disease, celebrada en Madrid (España) del 4 al 5 de noviembre de 2016.Plakins are large multi-domain proteins that interconnect cytoskeletal structures. Plectin is a prototypical plakin that tethers intermediate filaments to membrane-associated complexes. For example in epithelia plectin links the cytokeratins to the integrin α6ß4 in the hemidesmosomes. The N-terminal region of plectin contains an actin binding domain that mediates the binding to α6ß4, and a region named the plakin domain that is conserved in most plakins. The plakin domain of plectin is formed by nine spectrin repeats (SR1 to SR9) and a non-canonical SH3 domain. We have combined X-ray crystallography with small angle X-ray scattering (SAXS) to elucidate the global structure of the plakin domain of plectin, extending our previous analysis of the SR1 to SR5 region. Two crystal structures of the SR5-SR6 segment allowed us to characterize its uniquely wide inter-repeat conformational variability. We also report the crystal structures of the SR7-SR8 region, refined to 1.8 Å, and the SR7-SR9 at lower resolution. The SR7-SR9 region, which is conserved in all other plakin domains, forms a rigid segment stabilized by uniquely extensive inter-repeat contacts mediated by unusually long helices in SR8 and SR9. Using SAXS we show that in solution the SR3-SR9 of plectin has an extended shape with a small central kink. Other plakins, such as bullous pemphigoid antigen 1 (BPAG1) and microtubule and actin crosslinking factor 1 (MACF1), are likely to have similar extended plakin domains. The array of SRs in the plakin domain is very similar to those present in spectrins. Such arrays of SRs form deformable structures that can be bent; SRs can also unfold individually at low pulling forces. The continuous and extended rod-like structure of plectin suggests that the plakin domain may work as a molecular shock absorbent that dissipates elastic energy when cells are subjected to external forces, and might contribute to the mechanical stability and resilience of tissues that are subjected to mechanical stressPeer Reviewe