Evaluation of advanced backcrosses of eggplant with Solanum elaeagnifolium introgressions under low N conditions

[EN] Selection and breeding of eggplant (Solanum melongena) materials with good performance under low nitrogen (N) fertilization inputs is a major breeding objective to reduce environmental degradation, risks for human health, and production costs. Solanum elaeagnifolium, an eggplant wild relative, is a potential source of variation for introgression breeding in eggplant. We evaluated 24 plant, fruit, and composition traits in a set of genotyped advanced backcrosses (BC2 and BC3) of eggplant with S. elaeagnifolium introgressions under low N conditions. Significant differences were found between the two parents for most traits, and a wide phenotypic diversity was observed in the advanced backcrosses, with some individuals with a much higher yield, nitrogen use efficiency (NUE), and phenolics content than the S. melongena parent. In general, the lower the proportion of S. elaeagnifolium genome introgressed in the advanced backcrosses, the higher was the general phenotypic resemblance to S. melongena. Putative QTLs were detected for stem diameter (pd4), presence of prickles in stem (ps6), leaf (pl6) and fruit calyx (pc6), fruit width (fw7), chlorogenic acid content (cg5), total phenolic acid peaks area (ph6), chlorogenic acid peak area (ca1), and phenolic acids pattern (cp1). Our results reveal that introgression breeding of eggplant with S. elaeagnifolium has a great interest for eggplant breeding, particularly for adaptation to low N conditions. These materials can potentially contribute to the development of improved eggplant varieties for a more sustainable agriculture.This work was supported by the project SOLNUE in the framework of the H2020 call SusCrop-ERA-Net (ID#47) and funded by Agencia Estatal de Investigación (PCI2019-103375) and by the Ministerio de Ciencia, Innovación y Universidades, Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional (grant RTI2018-094592-B-I00 from MCIU/AEI/ FEDER, UE). The Spanish Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación, and Fondo Social Europeo funded a predoctoral fellowship to Gloria Villanueva (PRE2019-103375). The Spanish Ministerio de Economía y Competitividad, Agencia Estatal de Investigación, and Fondo Social Europeo funded a predoctoral fellowship to Elena Rosa-Martínez (BES-2016-077482) and a postdoctoral fellowship to Mariola Plazas (IJC2019-039091-I).Villanueva-Párraga, G.; Rosa-Martínez, E.; Sahin, A.; García-Fortea, E.; Plazas Ávila, MDLO.; Prohens Tomás, J.; Vilanova Navarro, S. (2021). Evaluation of advanced backcrosses of eggplant with Solanum elaeagnifolium introgressions under low N conditions. Agronomy. 11(9):1-18. https://doi.org/10.3390/agronomy11091770S11811

Fruit Composition of Eggplant Lines with Introgressions from the Wild Relative S. incanum: Interest for Breeding and Safety for Consumption

[EN] The wild species Solanum incanum has been used as a donor parent for the development of a set of eggplant introgression lines (ILs), which are of interest for breeding for stress tolerances and relevant morpho-agronomic traits but could also be useful for breeding for fruit quality, due to the generally higher content in health-promoting compounds of S. incanum. The use of eggplant ILs with introgressions from S. incanum requires ensuring that glycoalkaloids levels are below safety limits. We evaluated 25 fruit composition traits, including proximate composition, sugars, acids, phenolics, glycoalkaloids, and minerals in a set of 16 eggplant ILs with S. incanum, both parents and the F-1, grown under two environments (open field and screenhouse). The results demonstrated that the parents were significantly different regarding most fruit composition traits. Large variation was found among the 16 ILs for all traits analyzed and a strong influence of the environment accounted for the variation of 17 out of the 25 traits evaluated. Although the S. incanum parent produced fruits with high levels of glycoalkaloids, the 16 ILs showed mean values of total glycoalkaloids below the currently accepted safety limit for human consumption (200 mg kg(-1) fresh weight). Overall, the ILs produced fruits that are safe for consumption, with nutritional and functional quality similar to the recipient parent. Furthermore, six putative QTLs were detected spread over chromosomes 3 for crude protein, 5 for malic and total acids, and 7 for chlorogenic acid and solamargine, and potential candidate genes were spotted for most of them, which provide new relevant information for eggplant breeding.This work was undertaken as part of the initiative "Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives", which is supported by the Government of Norway. The project is managed by the Global Crop Diversity Trust with the Millennium Seed Bank of the Royal Botanic Gardens, Kew, and implemented in partnership with national and international gene banks and plant breeding institutes around the world. For further information see the project website: http://www.cwrdiversity.org/.Funding was also received from grants AGL2015-64755-R funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe" and RTI-2018-094592-B-100 funded by MCIN/AEI/10.13039/501100011033, and from the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 677379 (G2P-SOL project: Linking genetic resources, genomes, and phenotypes of Solanaceous crops). M.P. is grateful to the Spanish Ministerio de Ciencia e Innovacion for a post-doctoral grant with the Juan de la Cierva program (grant number IJC2019-039091-I from MCIN/AEI/10.1309/501100011033). P.G. is grateful to the Japan Society for the Promotion of Science for a post-doctoral grant (P19105, FY2019 JSPS Postdoctoral Fellowship for Research in Japan). E.R.-M. is grateful to the Spanish Ministerio de Economia, Industria y Competitividad for a pre-doctoral grant (grant number BES-2016-077482 from MCIN/AEI/10.1309/501100011033).Rosa-Martínez, E.; Adalid-Martinez, AM.; García-Martínez, MD.; Mangino, G.; Raigón Jiménez, MD.; Plazas Ávila, MDLO.; Gramazio, P.... (2022). Fruit Composition of Eggplant Lines with Introgressions from the Wild Relative S. incanum: Interest for Breeding and Safety for Consumption. Agronomy. 12(2):1-12. https://doi.org/10.3390/agronomy1202026611212

Fruit composition diversity in land races and modern pepino (Solanum muricatum) varieties and wild related species

[EN] Pepino (Solanum muricatum) fruits from 15 accessions of cultivated pepino as well as six accessions from wild relatives were evaluated for contents in dry matter, protein, b-carotene, chlorophylls and seven minerals. Several-fold differences among accessions were found for most traits. Average values obtained were similar to those of melon and cucumber, but the phenolic contents were much higher. Wild species had significantly higher average contents for all traits vs. the cultivated pepino accessions. And, the comparisons among the cultivated pepino varieties showed that the modern varieties were more uniform in composition, and they possessed significantly lower concentrations of protein, P, K, and Zn than local land races. Most of the significant correlations among composition traits were positive. Our studies show that regular consumption of pepino fruits could make a significant contribution to the recommended daily intake of P, K, Fe and Cu as well as to the average daily intake of phenolics. Furthermore, the higher values for most nutrients measured in the wild species and in the local land races indicate that new pepino varieties with improved fruit contents in nutrient and bioactive compounds can be developed.Pietro Gramazio is grateful to Universitat Politecnica de Valencia for a pre-doctoral (Programa FPI de la UPV-Subprograma 1/2013 call) contract.Herraiz García, FJ.; Raigón Jiménez, MD.; Vilanova Navarro, S.; García-Martínez, MD.; Gramazio, P.; Plazas Ávila, MDLO.; Rodríguez Burruezo, A.... (2016). Fruit composition diversity in land races and modern pepino (Solanum muricatum) varieties and wild related species. Food Chemistry. 203:49-58. https://doi.org/10.1016/j.foodchem.2016.02.035S495820

Fruit composition profile of pepper, tomato and eggplant varieties grown under uniform conditions

[EN] The study of the diversity within and between major Solanaceae crops (pepper, tomato, eggplant) is of interest for the selection and development of balanced diets. We have measured thirty-six major fruit composition traits, encompassing sugars, organic acids, antioxidants and minerals, in a set of 10 accessions per crop for pepper, tomato and eggplant, grown under the same cultivation conditions. The aim was to evaluate the diversity within species and to provide an accurate comparison of fruit composition among species by reducing to a minimum the environmental effect. Pepper, tomato and eggplant had a clearly distinct composition profile. Pepper showed the highest average content in total sugars and organic acids. Fructose and glucose were the major sugar compounds in the three species, although in pepper and tomato sucrose was present only in trace amounts. Citric acid was the major organic acid in pepper and tomato, while in eggplant it was malic acid. Pepper and eggplant had the highest total antioxidant activity. Vitamin C content was much higher in pepper than in tomato and eggplant, while eggplant accumulated high concentrations of chlorogenic acid. Furthermore, eggplant was the species with higher content in most minerals, particularly for K, Mg and Cu, while pepper was the richest in Fe. Due to their complementary nutritional profiles, a combined regular consumption of the three vegetables would supply more than 20% of the Dietary Reference Intake of several of the analysed phytochemicals. The large diversity within each species is of interest for selecting varieties with better nutritional and organoleptic profiles, as well as for breeding new cultivars.This work has been funded by the European Union's Horizon 2020 Research and Innovation Programme under grant agreement No. 677379 (Linking genetic resources, genomes and phenotypes of Sola-naceous crops; G2PSOL) . Elena RosaMartinez is grateful to the Spanish Ministerio de Economia, Industria y Competitividad for a pre-doctoral grant (BES2016077482)Rosa-Martínez, E.; García-Martínez, MD.; Adalid-Martinez, AM.; Pereira-Días, L.; Casanova-Calancha, C.; Soler-Calabuig, E.; Figás-Moreno, MDR.... (2021). Fruit composition profile of pepper, tomato and eggplant varieties grown under uniform conditions. Food Research International. 147:1-13. https://doi.org/10.1016/j.foodres.2021.110531S11314

Genetic structure of Cannabis sativa var. indica cultivars based on genomic SSR (gSSR) markers: implications for breeding and germplasm management

[EN] Cannabis saliva L. is cultivated for its fiber or seeds (var. saliva; hemp), or for its high content in cannabinoids (var. indica; marijuana). Knowledge of the genetic structure of C. sativa var. indica is important for selection and breeding of cultivars with medicinal interest. We used six genomic SSRs (gSSRs) for genotyping 154 individual plants of 20 cultivars of C. sativa var. indica, plus two cultivars of C. sativa var. sativa. A very high polymorphism was observed, with an average of 17 alleles and 23.8 genotypes per locus. Expected (H-e) and observed (H-o) heterozygosities were high, with average values of 0.753 and 0.429, respectively. In some cultivars H-e and H-o presented similar values, while in others H-e was considerably higher than H-o suggesting that consanguinity and fixation had taken place during its development. In addition, some cultivars had a reduced number of alleles per locus (in some cases only two) indicating that a genetic bottleneck had taken place during its development. Gene flow (Nm) between both botanical varieties was high, with Nm = 1.736. The molecular analysis of variance (AMOVA) revealed that only 31.94% of the molecular variation observed was caused by differences among cultivars, while the variation among plants of the same cultivar was of 37.11%, and within individual variation, due to heterozygosity, was of 30.96%. This indicates that a large variation exists within cultivars, which can be exploited for selection, but also complicates germplasm management and regeneration. The population structure analysis identified 14 genetic clusters, with most individuals of a single cultivar clustering together. This analysis, together with UPGMA cluster analysis shows that the two C. saliva var. saliva cultivars studied are differentiated from C. sativa var. indica, and that some cultivars of C. saliva var. indica seem to represent different selections from a common original cultivar. Our results represent the first comprehensive study of intra-varietal diversity in C. sativa var. indica and provide information of relevance for selection, breeding, and germplasm conservation, as well as for forensic studies in this crop.Soler Aleixandre, S.; Gramazio, P.; Figás-Moreno, MDR.; Vilanova Navarro, S.; Rosa-Martínez, E.; Llosa, ER.; Borràs-Palomares, D.... (2017). Genetic structure of Cannabis sativa var. indica cultivars based on genomic SSR (gSSR) markers: implications for breeding and germplasm management. Industrial Crops and Products. 104:171-178. doi:10.1016/j.indcrop.2017.04.043S17117810

Variation for Composition and Quality in a Collection of the Resilient Mediterranean 'de penjar' Long Shelf-Life Tomato Under High and Low N Fertilization Levels

[EN] The 'de penjar' tomato (Solanum lycopersicum L.) is a group of local varieties from the Spanish Mediterranean region carrying the alc mutation, which provides long shelf-life. Their evolution under low-input management practices has led to the selection of resilient genotypes to adverse conditions. Here we present the first evaluation on nutritional fruit composition of a collection of 44 varieties of 'de penjar' tomato under two N fertilization levels, provided by doses of manure equivalent to 162 kg N ha(-1) in the high N treatment and 49 kg N ha(-1) in the low N treatment. Twenty-seven fruit composition and quality traits, as well as plant yield and SPAD value, were evaluated. A large variation was observed, with lycopene being the composition trait with the highest relative range of variation (over 4-fold) under both N treatments, and significant differences among varieties were detected for all traits. While yield and most quality traits were not affected by the reduction in N fertilization, fruits from the low N treatment had, on average, higher values for hue (5.9%) and lower for fructose (-11.5%), glucose (-15.8%), and total sweetness index (-12.9%). In addition, lycopene and beta-carotene presented a strongly significant genotype x N input interaction. Local varieties had higher values than commercial varieties for traits related to the ratio of sweetness to acidity and for vitamin C, which reinforces the appreciation for their organoleptic and nutritional quality. Highest-yielding varieties under both conditions displayed wide variation in the composition and quality profiles, which may allow the selection of specific ideotypes with high quality under low N conditions. These results revealed the potential of 'de penjar' varieties as a genetic resource in breeding for low N inputs and improving the organoleptic and nutritional tomato fruit quality.This work has been funded by the European Union's Horizon 2020 Research and Innovation Programme under grant agreement no. 774244 (Breeding for resilient, efficient and sustainable organic vegetable production; BRESOV), by the Spanish Agencia Estatal de Investigacion under grant agreement no. PCI2019-103375 (project SOLNUE in the framework of the H2020 call SusCrop-ERA-Net; ID#47), and by Generalitat Valenciana (Conselleria d'Innovacio, Universitats, Ciencia I Societat Digital) under grant agreement no. AICO/2020/042. ER-M is grateful to the Spanish Ministerio de Economia, Industria y Competitividad for a pre-doctoral grant (BES-2016-077482).Rosa-Martínez, E.; Adalid-Martinez, AM.; Alvarado, LE.; Burguet-Belda, R.; García-Martínez, MD.; Pereira-Días, L.; Casanova-Calancha, C.... (2021). Variation for Composition and Quality in a Collection of the Resilient Mediterranean 'de penjar' Long Shelf-Life Tomato Under High and Low N Fertilization Levels. Frontiers in Plant Science. 12:1-19. https://doi.org/10.3389/fpls.2021.6339571191

SDSS-IV MaNGA : the MaNGA dwarf galaxy sample presentation

M.C.D. acknowledges support from CONACYT "Ciencia de Frontera" grant 320199. M.C.D. and H.M.H.T. acknowledge support from UC MEXUS-CONACYT grant CN-17-128. A.R.P. acknowledges support from the CONACyT "Ciencia Basica" grant 285721. E.A.O. acknowledges support from the SECTEI (Secretaría de Educación, Ciencia, Tecnología e Innovación de la Ciudad de México) under the Postdoctoral Fellowship SECTEI/170/2021 and CM-SECTEI/303/2021. Funding for the Sloan Digital Sky Survey IV has been provided by the Alfred P. Sloan Foundation, the U.S. Department of Energy Office of Science, and the Participating Institutions.We present the MaNGA Dwarf galaxy (MaNDala) Value Added Catalog (VAC), from the final release of the Sloan Digital Sky Survey-IV program. MaNDala consists of 136 randomly selected bright dwarf galaxies with M* −18.5, making it the largest integral field spectroscopy homogeneous sample of dwarf galaxies. We release a photometric analysis of the g, r, and z broadband imaging based on the DESI Legacy Imaging Surveys, as well as a spectroscopic analysis based on the Pipe3D SDSS-IV VAC. Our release includes the surface brightness (SB), geometric parameters, and color profiles, Sérsic fits as well as stellar population properties (such as stellar ages, metallicities, and star formation histories), and emission lines' fluxes within the FOV and the effective radii of the galaxies. We find that the majority of the MaNDala galaxies are star-forming late-type galaxies with 〈nSersic,r〉∼1.6 that are centrals (central/satellite dichotomy). MaNDala covers a large range of SB values (we find 11 candidate ultra-diffuse galaxies and three compact ones), filling the gap between classical dwarfs and low-mass galaxies in the Kormendy Diagram and in the size–mass/luminosity relation, which seems to flatten at 108 2, while the last 20% was at 〈z〉 < 0.3. Finally, a bending of the sSFR-M * relation at M* ∼ 109 M⊙ for the main-sequence galaxies seems to be supported by MaNDala.Publisher PDFPeer reviewe

Modifiable risk factors associated with prediabetes in men and women: A cross-sectional analysis of the cohort study in primary health care on the evolution of patients with prediabetes

Background: Prediabetes is a high-risk state for diabetes development, but little is known about the factors associated with this state. The aim of the study was to identify modifiable risk factors associated with the presence of prediabetes in men and women. Methods: Cohort Study in Primary Health Care on the Evolution of Patients with Prediabetes (PREDAPS-Study) is a prospective study on a cohort of 1184 subjects with prediabetes and another cohort of 838 subjects without glucose metabolism disorders. It is being conducted by 125 general practitioners in Spain. Data for this analysis were collected during the baseline stage in 2012. The modifiable risk factors included were: smoking habit, alcohol consumption, low physical activity, inadequate diet, hypertension, dyslipidemia, and obesity. To assess independent association between each factor and prediabetes, odds ratios (ORs) were estimated using logistic regression models. Results: Abdominal obesity, low plasma levels of high-density lipoprotein cholesterol (HDL-cholesterol), and hypertension were independently associated with the presence of prediabetes in both men and women. After adjusting for all factors, the respective ORs (95% Confidence Intervals) were 1.98 (1.41-2.79), 1.88 (1.23-2.88) and 1.86 (1.39-2.51) for men, and 1.89 (1.36-2.62), 1.58 (1.12-2.23) and 1.44 (1.07-1.92) for women. Also, general obesity was a risk factor in both sexes but did not reach statistical significance among men, after adjusting for all factors. Risky alcohol consumption was a risk factor for prediabetes in men, OR 1.49 (1.00-2.24). Conclusions: Obesity, low HDL-cholesterol levels, and hypertension were modifiable risk factors independently related to the presence of prediabetes in both sexes. The magnitudes of the associations were stronger for men than women. Abdominal obesity in both men and women displayed the strongest association with prediabetes. The findings suggest that there are some differences between men and women, which should be taken into account when implementing specific recommendations to prevent or delay the onset of diabetes in adult population

Physical Activity Patterns of the Spanish Population Are Mostly Determined by Sex and Age: Findings in the ANIBES Study

Background Representative data for the Spanish population regarding physical activity (PA) behaviors are scarce and seldom comparable due to methodological inconsistencies. Aim Our objectives were to describe the PA behavior by means of the standardized self-reported International Physical Activity Questionnaire (IPAQ) and to know the proportion of the Spanish population meeting and not meeting international PA recommendations. Material and Methods PA was assessed using the IPAQ in a representative sample of 2285 individuals (males, 50.4%) aged 9–75 years and living in municipalities of at least 2,000 inhabitants. Data were analyzed according to: age groups 9–12, 13–17, 18–64, and 65–75 years; sex; geographical distribution; locality size and educational levels. Results Mean total PA was 868.8±660.9 min/wk, mean vigorous PA 146.4±254.1 min/wk, and mean moderate PA 398.1±408.0 min/wk, showing significant differences between sexes (p<0.05). Children performed higher moderate-vigorous PA than adolescents and seniors (p<0.05), and adults than adolescents and seniors (p<0.05). Compared to recommendations, 36.2%of adults performed <150 min/week of moderate PA, 65.4% <75 min/week of vigorous PA and 27.0%did not perform any PA at all, presenting significant differences between sexes (p<0.05). A total of 55.4%of children and adolescents performed less than 420 min/week of MVPA, being higher in the later (62.6%) than in the former (48.4%). Highest non-compliance was observed in adolescent females (86.5%). Conclusion Sex and age are the main influencing factors on PA in the Spanish population. Males engage in more vigorous and light PA overall, whereas females perform more moderate PA. PA behavior differs between age groups and no clear lineal increase with age could be observed. Twenty-seven percent of adults and 55.4% of children and adolescents do not meet international PA recommendations. Identified target groups should be addressed to increase PA in the Spanish populationCoca-Cola Iberia through Spanish Nutrition Foundation (FEN)Coca-Cola Iberi