Monitoring mountain cryosphere dynamics by time-lapse stereo-photogrammetry

International audienc

Short-term effect of aldosterone on vasopressin-sensitive adenylate cyclase in rat collecting tubule

International audienceBecause previous studies indicated that, in the rat collecting tubule, vasopressin (AVP)-sensitive adenylate cyclase (AC) is controlled by mineralocorticoids in the long term, the present study was designed to investigate whether such a control also exists in the short term. Therefore, we investigated the in vivo and in vitro effects of aldosterone on AC activity in cortical and outer medullary collecting tubules (CCD and OMCD, respectively) from adrenalectomized rats. Injection of aldosterone (10 micrograms/kg body wt) to adrenalectomized rats restored within 3 h AVP-sensitive AC activity in the CCD and OMCD up to the levels observed in the corresponding segments of adrenal intact rats. Similarly, incubating CCD or OMCD from adrenalectomized rats for 2.5 h in the presence of 10(-8) M aldosterone enhanced AVP-sensitive AC activity up to values similar to those found in normal rats. In vitro stimulation of AVP-sensitive AC activity was dose dependent with regard to aldosterone [apparent affinity constant (K0.5) approximately 10(-9) M], appeared after a 30-min lag period, and reached its maximum after 2-2.5 h. In addition, it was totally abolished by the antimineralocorticoid spironolactone, whereas the specific glucocorticoid antagonist RU 38486 had no effect. Finally, actinomycin D and cycloheximide totally abolished the in vitro action of aldosterone, demonstrating the involvement of protein synthesis in that process

EVALUATING the QUALITY of PHOTOGRAMMETRIC POINT-CLOUDS in CHALLENGING GEO-ENVIRONMENTS - A CASE STUDY in AN ALPINE VALLEY

Precise and accurate three-dimensional geospatial data has become increasingly available thanks to advances in both Terrestrial Laser Scanning (TLS) and Structure-from-Motion Photogrammetry (SfM). These tools provide valuable information for mapping geomorphological features and detect surface changes in mountainous environments. The exploitation of 3D point-clouds has been proven tremendously useful in the field of geosciences. It remains, however, controversial whether cost efficient photogrammetry can provide as accurate and reliable geospatial information as the significantly more expensive laser scanning or not. In this study, a rockfall case site in the territory of Obergurgl, Austria, is investigated in order to provide answers to the above question in a complex environment. The analysis includes different terrestrial photogrammetry configurations aiming to comprehensively define the strengths and limitations of terrestrial photogrammetry over TLS. The latter constitutes an optimized methodology that provides guidelines for costly future assessments as part of the site investigation phase in geohazard management. There are no doubts that compared to traditional and conventional surveying methods TLS and Photogrammetry both offer products much faster and with a much higher data density. In the current study, we show that when photogrammetry is applied following a well-defined optimized strategy, it can be potentially an adequate alternative to more costly TLS datasets for mass movement assessment and monitoring purposes

Investigating the slope failures at the Lou rock glacier front, French Alps

International audienceOn August 14 th 2015 a large debris flow initiated by the occurrence of two slope failures at the front of the Lou rock glacier flooded part of the town of Lanslevillard, France. The present study aims to understand the meteorological and geomorphological context that led to these failures. Investigations were conducted by combining meteorological data, surface movements, and geophysical transects. The analysis indicates that the Lou rock glacier is directly connected to an active torrential channel and has a natural predisposition to frontal failure due to the steepness of its front. The slope failures were triggered after a heat wave followed by a three-week period of almost continuous rainfall. Water flowing on top of the permafrost table was observed in the two head scarps, suggesting that regressive erosion consecutive to this concentrated subsurface water flow triggered the failures. For one of the slides, traces of previous failures were observable on historical aerial imagery dating back to the 1950's, while the second slide corresponded to a novel event and developed on the frontal slope of a fast-moving and destabilized rock glacier lobe. We also discuss the increase in local predisposition to failure related to the remarkable morphological modifications such as frontal advance and development of surface cracks associated with the lobe destabilization

Evaluating the quality of photogrammetric point-clouds in challenging geo-environments: a case study in an Alpine valley

Precise and accurate three-dimensional geospatial data has become increasingly available thanks to advances in both Terrestrial Laser Scanning (TLS) and Structure-from-Motion Photogrammetry (SfM). These tools provide valuable information for mapping geomorphological features and detect surface changes in mountainous environments. The exploitation of 3D point-clouds has been proven tremendously useful in the field of geosciences. It remains, however, controversial whether cost efficient photogrammetry can provide as accurate and reliable geospatial information as the significantly more expensive laser scanning or not. In this study, a rockfall case site in the territory of Obergurgl, Austria, is investigated in order to provide answers to the above question in a complex environment. The analysis includes different terrestrial photogrammetry configurations aiming to comprehensively define the strengths and limitations of terrestrial photogrammetry over TLS. The latter constitutes an optimized methodology that provides guidelines for costly future assessments as part of the site investigation phase in geohazard management. There are no doubts that compared to traditional and conventional surveying methods TLS and Photogrammetry both offer products much faster and with a much higher data density. In the current study, we show that when photogrammetry is applied following a well-defined optimized strategy, it can be potentially an adequate alternative to more costly TLS datasets for mass movement assessment and monitoring purposes

Mass Spectrometry of Mitochondrial Membrane Protein Complexes

International audienceThe ATP production (oxidative phosphorylation) involves five complexes embedded in the inner membrane of mitochondria. The yeast Saccharomyces cerevisiae is mainly used as a model for the study of oxidative phosphorylation; mutants are easy to produce and are still viable due to their ability to grow using the fermentation pathway. Here we present a process for analyzing mitochondrial respiratory complexes using native electrophoresis (BN-PAGE) coupled to LC-MS/MS. BN-PAGE (i) permits the separation of functional respiratory complexes, thus allowing in-gel activity detection of most of the respiratory complexes and (ii) provides convenient samples for bottom-up proteomics. Combining BN-PAGE and LC-MS/MS leads to the identification of the subunit composition of membrane complexes and offers the possibility to highlight potential interacting proteins

Pesquisa de antigenos aglutinantes "major" 1, 2 e 3 em cepas de Bordetella pertussis, isoladas de crianças com coqueluche atendidas no Hospital de Isolamento Emílio Ribas de São Paulo, Brasil Determination of 1, 2 and 3 major antigens in Bordetella pertussis strains isolated from Brazilian children with whooping-cough

Em 30 cepas de Bordetella pertussis isoladas de crianças com coqueluche, atendidas no Hospital de Isolamento Emílio Ribas de São Paulo, foram pesquisados os antígenos aglutinantes ''major" 1, 2 e 3. Levando-se em conta a presença combinada dos três antígenos, as provas de soro-aglutinação rápida em lamina revelaram que 25 (83,3%) cepas possuiam os fatores 1, 2 e 3, enquanto que 3 (10,0%) e 2 (6,7%) foram positivas, somente, para 1, 2 e 1, 3, respectivamente. Os resultados foram discutidos, considerando-se a importância deste antígeno no preparo de vacinas.<br>The presence of major antigens, 1, 2 and 3 were determined in 30 strains of B. pertussis isolated from children with whooping-cough hospitalized at the Hospital Emílio Ribas, São Paulo Brazil. The method used was the slide-agglutination test. Tests showed that 25(83.3%) of strains were positives for factors 1, 2 and 3. Factores 1 and 3 alone were present in 3 (10%) of strains and 1 and 2 alone in 2 (6.7%)