Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Estimating Infrared Radiometric Satellite Sea Surface Temperature Retrieval Cold Biases in the Tropics due to Unscreened Optically Thin Cirrus Clouds

Passive longwave infrared radiometric satellite–based retrievals of sea surface temperature (SST) at instrument nadir are investigated for cold bias caused by unscreened optically thin cirrus (OTC) clouds [cloud optical depth (COD) ≤ 0.3]. Level 2 nonlinear SST (NLSST) retrievals over tropical oceans (30°S–30°N) from Moderate Resolution Imaging Spectroradiometer (MODIS) radiances collected aboard the NASA Aqua satellite (Aqua-MODIS) are collocated with cloud profiles from the Cloud–Aerosol Lidar with Orthogonal Polarization (CALIOP) instrument. OTC clouds are present in approximately 25% of tropical quality-assured (QA) Aqua-MODIS Level 2 data, representing over 99% of all contaminating cirrus found. Cold-biased NLSST (MODIS, AVHRR, and VIIRS) and triple-window (AVHRR and VIIRS only) SST retrievals are modeled based on operational algorithms using radiative transfer model simulations conducted with a hypothetical 1.5-km-thick OTC cloud placed incrementally from 10.0 to 18.0 km above mean sea level for cloud optical depths between 0.0 and 0.3. Corresponding cold bias estimates for each sensor are estimated using relative Aqua-MODIS cloud contamination frequencies as a function of cloud-top height and COD (assuming they are consistent across each platform) integrated within each corresponding modeled cold bias matrix. NLSST relative OTC cold biases, for any single observation, range from 0.33° to 0.55°C for the three sensors, with an absolute (bulk mean) bias between 0.09° and 0.14°C. Triple-window retrievals are more resilient, ranging from 0.08° to 0.14°C relative and from 0.02° to 0.04°C absolute. Cold biases are constant across the Pacific and Indian Oceans. Absolute bias is lower over the Atlantic but relative bias is higher, indicating that this issue persists globally