130 research outputs found
Practical recommendations to communicate with patients about healthârelated conspiracy theories
Health-related conspiracy theories often advance the argument that information is being kept secret from the public by powerful individuals or groups within the government or health industry. They are widespread and are associated with important health attitudes, intentions, and behaviours. Recent research suggests that individuals are attracted to conspiracy theories to satisfy three important and fundamental psychological needs: epistemic, existential, and social needs. Understanding these underlying motivations associated with health-related conspiracy theories can help address patientsâ beliefs. Debunking and inoculation are discussed as approaches that can be used to address patientsâ belief in health-related conspiracy theories
Motivations for social media use: Associations with social media engagement and body satisfaction and well-being among adolescents
Adolescents are spending considerable time on social media, yet it is unclear whether motivations for social media use drive different forms of social media engagement, and their relationships with body satisfaction and well-being. This study tested a proposed model of the relationships between motivations for social media use, types of social media engagement and body satisfaction and well-being. Responses to an online survey from 1432 Australian adolescents (M = 13.45 years, SD = 1.14, range 11â17; 55.4% boys) were collected. Structural equational modelling indicates excellent model fit. Specifically, motivations for social media use (information sharing, passing time, escapism, social interaction, social capital and appearance feedback) were associated with engagement (intensity, photo-based use, active use, passive use and liking use) and revealed mixed associations with body satisfaction and well-being. The findings support the importance of considering motivations for social media use in future research. ag
Tribocorrosion behavior of TiâCâOâN nanostructured thin films (black) for decorative applications
In the past few years, tribocorrosion has become a focus of research because of its relevance in terms of the future in-service degradation mechanisms of materials. In the particular case of decorative coatings, tribocorrosion is certainly one of the most important issues, and sweat corrosion and human contact wear are two other factors that may act as material selection tools.
Thus, the current study aimed to investigate the tribocorrosion behavior of a new class of thin films, the TiâCâOâN system, which is being developed to be used as a surface decorative material due to its relatively dark appearance. The films were prepared by reactive magnetron sputtering. The influence of the structural features on the tribocorrosion behavior is discussed.This research is supported by FEDER funds through COMPETE-Programa Operacional Factores de Competitividade and by national funds through the Fundacao para a Ciencia e a Tecnologia, project PTDC/CTM/69362/2006 and contract SFRH/BD/27569/2006
Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria
Acetate is a short-chain fatty acid secreted by Propionibacteria from the human intestine, known to induce mitochondrial apoptotic death in colorectal cancer (CRC) cells. We previously established that acetate also induces lysosome membrane permeabilization in CRC cells, associated with release of the lysosomal protease cathepsin D (CatD), which has a well-established role in the mitochondrial apoptotic cascade. Unexpectedly, we showed that CatD has an antiapoptotic role in this process, as pepstatin A (a CatD inhibitor) increased acetate-induced apoptosis. These results mimicked our previous data in the yeast system showing that acetic acid activates a mitochondria-dependent apoptosis process associated with vacuolar membrane permeabilization and release of the vacuolar protease Pep4p, ortholog of mammalian CatD. Indeed, this protease was required for cell survival in a manner dependent on its catalytic activity and for efficient mitochondrial degradation independently of autophagy. In this study, we therefore assessed the role of CatD in acetate-induced mitochondrial alterations. We found that, similar to acetic acid in yeast, acetate-induced apoptosis is not associated with autophagy induction in CRC cells. Moreover, inhibition of CatD with small interfering RNA or pepstatin A enhanced apoptosis associated with higher mitochondrial dysfunction and increased mitochondrial mass. This effect seems to be specific, as inhibition of CatB and CatL with E-64d had no effect, nor were these proteases significantly released to the cytosol during acetate-induced apoptosis. Using yeast cells, we further show that the role of Pep4p in mitochondrial degradation depends on its protease activity and is complemented by CatD, indicating that this mechanism is conserved. In summary, the clues provided by the yeast model unveiled a novel CatD function in the degradation of damaged mitochondria when autophagy is impaired, which protects CRC cells from acetate-induced apoptosis. CatD inhibitors could therefore enhance acetate-mediated cancer cell death, presenting a novel strategy for prevention or therapy of CRC.FEDER through POFC â COMPETE and by Fundação para a CiĂȘncia e Tecnologia through projects PEst-OE/BIA/UI4050/2014 and FCT ANR/BEX-BCM/0175/201
The Atacama Cosmology Telescope: Cosmology from cross-correlations of unWISE galaxies and ACT DR6 CMB lensing
We present tomographic measurements of structure growth using
cross-correlations of Atacama Cosmology Telescope (ACT) DR6 and Planck CMB
lensing maps with the unWISE Blue and Green galaxy samples, which span the
redshift ranges and , respectively. We improve on prior unWISE cross-correlations not just by
making use of the new, high-precision ACT DR6 lensing maps, but also by
including additional spectroscopic data for redshift calibration and by
analysing our measurements with a more flexible theoretical model. An extensive
suite of systematic and null tests within a blind analysis framework ensures
that our results are robust. We determine the amplitude of matter fluctuations
at low redshifts (), finding using the ACT cross-correlation alone and with a combination of Planck and ACT cross-correlations; these
measurements are fully consistent with the predictions from primary CMB
measurements assuming standard structure growth. The addition of Baryon
Acoustic Oscillation data breaks the degeneracy between and
, allowing us to measure from the
cross-correlation of unWISE with ACT and from the
combination of cross-correlations with ACT and Planck. These results also agree
with the expectations from primary CMB extrapolations in CDM
cosmology; the consistency of derived from our two redshift samples
at and provides a further check of our cosmological model.
Our results suggest that structure formation on linear scales is well described
by CDM even down to low redshifts .Comment: 73 pages (incl. 30 pages of appendices), 50 figures, 16 tables, to be
submitted to ApJ. Watch G. S. Farren and A. Krolewski discuss the analysis
and results under https://cosmologytalks.com/2023/09/11/act-unwis
Mechanisms of TSC-mediated Control of Synapse Assembly and Axon Guidance
Tuberous sclerosis complex is a dominant genetic disorder produced by mutations in either of two tumor suppressor genes, TSC1 and TSC2; it is characterized by hamartomatous tumors, and is associated with severe neurological and behavioral disturbances. Mutations in TSC1 or TSC2 deregulate a conserved growth control pathway that includes Ras homolog enriched in brain (Rheb) and Target of Rapamycin (TOR). To understand the function of this pathway in neural development, we have examined the contributions of multiple components of this pathway in both neuromuscular junction assembly and photoreceptor axon guidance in Drosophila. Expression of Rheb in the motoneuron, but not the muscle of the larval neuromuscular junction produced synaptic overgrowth and enhanced synaptic function, while reductions in Rheb function compromised synapse development. Synapse growth produced by Rheb is insensitive to rapamycin, an inhibitor of Tor complex 1, and requires wishful thinking, a bone morphogenetic protein receptor critical for functional synapse expansion. In the visual system, loss of Tsc1 in the developing retina disrupted axon guidance independently of cellular growth. Inhibiting Tor complex 1 with rapamycin or eliminating the Tor complex 1 effector, S6 kinase (S6k), did not rescue axon guidance abnormalities of Tsc1 mosaics, while reductions in Tor function suppressed those phenotypes. These findings show that Tsc-mediated control of axon guidance and synapse assembly occurs via growth-independent signaling mechanisms, and suggest that Tor complex 2, a regulator of actin organization, is critical in these aspects of neuronal development
Oxidation of benzoin catalyzed by oxovanadium (IV) schiff base complexes
BACKGROUND: The oxidative transformation of benzoin to benzil has been accomplished by the use of a wide
variety of reagents or catalysts and different reaction procedures. The conventional oxidizing agents yielded mainly
benzaldehyde or/and benzoic acid and only a trace amount of benzil. The limits of practical utilization of these
reagents involves the use of stoichiometric amounts of corrosive acids or toxic metallic reagents, which in turn
produce undesirable waste materials and required high reaction temperatures.
In recent years, vanadium complexes have attracted much attention for their potential utility as catalysts for various
types of reactions.
RESULTS: Active and selective catalytic systems of new unsymmetrical oxovanadium(IV) Schiff base complexes for
the oxidation of benzoin is reported. The Schiff base ligands are derived between 2-aminoethanol and 2-hydroxy-1-
naphthaldehyde (H2L1) or 3-ethoxy salicylaldehyde (H2L3); and 2-aminophenol and 3-ethoxysalicylaldehyde (H2L2) or
2-hydroxy-1-naphthaldehyde (H2L4). The unsymmetrical Schiff bases behave as tridentate dibasic ONO donor
ligands. Reaction of these Schiff base ligands with oxovanadyl sulphate afforded the mononuclear oxovanadium(IV)
complexes (VIVOLx.H2O), which are characterized by various physico-chemical techniques.
The catalytic oxidation activities of these complexes for benzoin were evaluated using H2O2 as an oxidant. The best
reaction conditions are obtained by considering the effect of solvent, reaction time and temperature. Under the
optimized reaction conditions, VOL4 catalyst showed high conversion (>99%) with excellent selectivity to benzil
(~100%) in a shorter reaction time compared to the other catalysts considered.
CONCLUSION: Four tridentate ONO type Schiff base ligands were synthesized. Complexation of these ligands with
vanadyl(IV) sulphate leads to the formation of new oxovanadium(IV) complexes of type VIVOL.H2O.
Elemental analyses and spectral data of the free ligands and their oxovanadium(IV) complexes were found to be in
good agreement with their structures, indicating high purity of all the compounds.
Oxovanadium complexes were screened for the oxidation of benzoin to benzil using H2O2 as oxidant. The effect of
time, solvent and temperature were optimized to obtain maximum yield. The catalytic activity results demonstrate
that these catalytic systems are both highly active and selective for the oxidation of benzoin under mild reaction
conditions.Web of Scienc
The Atacama Cosmology Telescope: Mitigating the impact of extragalactic foregrounds for the DR6 CMB lensing analysis
We investigate the impact and mitigation of extragalactic foregrounds for the
CMB lensing power spectrum analysis of Atacama Cosmology Telescope (ACT) data
release 6 (DR6) data. Two independent microwave sky simulations are used to
test a range of mitigation strategies. We demonstrate that finding and then
subtracting point sources, finding and then subtracting models of clusters, and
using a profile bias-hardened lensing estimator, together reduce the fractional
biases to well below statistical uncertainties, with the inferred lensing
amplitude, , biased by less than . We also show
that another method where a model for the cosmic infrared background (CIB)
contribution is deprojected and high frequency data from Planck is included has
similar performance. Other frequency-cleaned options do not perform as well,
incurring either a large noise cost, or resulting in biased recovery of the
lensing spectrum. In addition to these simulation-based tests, we also present
null tests performed on the ACT DR6 data which test for sensitivity of our
lensing spectrum estimation to differences in foreground levels between the two
ACT frequencies used, while nulling the CMB lensing signal. These tests pass
whether the nulling is performed at the map or bandpower level. The
CIB-deprojected measurement performed on the DR6 data is consistent with our
baseline measurement, implying contamination from the CIB is unlikely to
significantly bias the DR6 lensing spectrum. This collection of tests gives
confidence that the ACT DR6 lensing measurements and cosmological constraints
presented in companion papers to this work are robust to extragalactic
foregrounds.Comment: Companion paper to Qu et al and Madhavacheril et a
The Influence of Bleached Jute Fiber Filler on the Properties of Vulcanized Natural Rubber
Identification of Conserved and HLA Promiscuous DENV3 T-Cell Epitopes
Anti-dengue T-cell responses have been implicated in both protection and immunopathology. However, most of the T-cell studies for dengue include few epitopes, with limited knowledge of their inter-serotype variation and the breadth of their human leukocyte antigen (HLA) affinity. In order to expand our knowledge of HLA-restricted dengue epitopes, we screened T-cell responses against 477 overlapping peptides derived from structural and non-structural proteins of the dengue virus serotype 3 (DENV3) by use of HLA class I and II transgenic mice (TgM): A2, A24, B7, DR2, DR3 and DR4. TgM were inoculated with peptides pools and the T-cell immunogenic peptides were identified by ELISPOT. Nine HLA class I and 97 HLA class II novel DENV3 epitopes were identified based on immunogenicity in TgM and their HLA affinity was further confirmed by binding assays analysis. A subset of these epitopes activated memory T-cells from DENV3 immune volunteers and was also capable of priming naïve T-cells, ex vivo, from dengue IgG negative individuals. Analysis of inter- and intra-serotype variation of such an epitope (A02-restricted) allowed us to identify altered peptide ligands not only in DENV3 but also in other DENV serotypes. These studies also characterized the HLA promiscuity of 23 HLA class II epitopes bearing highly conserved sequences, six of which could bind to more than 10 different HLA molecules representing a large percentage of the global population. These epitope data are invaluable to investigate the role of T-cells in dengue immunity/pathogenesis and vaccine design. © 2013 Nascimento et al
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