Storage problems of poplar chips from short rotation plantations with special emphasis on fungal development

There are several problems in storing wood chips freshly harvested from short rotation plantations, which result in quality losses as well as in dry matter and energy losses. The factors influencing the degradation of raw material are examined in this paper with special focus on fungal development. An excessive growth of fungi is connected to dry matter losses and also to an increased health risk during raw material handling. The following factors were measured during 6 months storage of poplar wood chips depending on particle size: box temperature, moisture content, pH-value, appearance of fungi in the storage and the concentration of fungal particles in the air. The results show a close connection between particle size, temperature and attack of fungi. During the storage mesophilic and termophilic species of the genera Alternaria, Aspergillus, Cladosporium, Mucor and Penicillium appeared. The concentration of fungal particles is the highest for fine chips and decreases in bigger particles. There was a special focus on the investigation of the properties of coarse chips (G 50), which represent a good compromise between handling, storage losses and health risk due to fungal development.Rövid vágásfordulójú nyár apríték tárolási problémái, különös tekintettel a kifejlődő gombákra. Frissen kitermelt rövid vágásfordulójú fafajokból előállított apríték tárolása során számos probléma merül fel, melyek eredménye minőség-, szárazanyag- és energiaveszteség. Ebben a tanulmányban a nyersanyag-degradációt befolyásoló tényezők kerültek vizsgálatra, különös tekintettel a kifejlődő gombákra. Ezek rendkívüli elszaporodásának köszönhető a szárazanyag-veszteség mellett a nyersanyag kezelése során fellépő, megnövekedett egészségügyi kockázat is. Nyár faapríték hat hónapos tárolása folyamán az aprítékméret függvényében a következő tényezők mérése zajlott: a tárolón belüli hőmérséklet, nedvességtartalom, pH, a tárolóban megjelenő gombák és azok száma a levegőben. Az eredmények szoros összefüggést mutatnak, különösen az aprítékméret, a hőmérséklet és a gombák száma közt. A tárolás során mezofil és termofil gombák fejlődtek. A következő nemzetségekhez tartozó fajok voltak megfigyelhetők: Alternaria, Aspergillus, Cladosporium, Mucor, Penicillium. A gombák száma a finom aprítéknál volt a legmagasabb, ami az aprítékméret növekedésével csökkent. A kutatás folyamán különös szerepet kapott a középfinom apríték tulajdonságainak vizsgálata (G 50), mely kompromisszumos megoldást jelent a kezelés, tárolási veszteségek és a kifejlődő gombáknak köszönhető egészségügyi kockázatok között

Storage Problems of Poplar Chips from Short Rotation Plantations with Special Emphasis on Fungal Development = Rövid vágásfordulójú nyár apríték tárolási problémái, különös tekintettel a kifejlődő gombákra

There are several problems in storing wood chips freshly harvested from short rotation plantations, which result in quality losses as well as in dry matter and energy losses. The factors influencing the degradation of raw material are examined in this paper with special focus on fungal development. An excessive growth of fungi is connected to dry matter losses and also to an increased health risk during raw material handling. The following factors were measured during 6 months storage of poplar wood chips depending on particle size: box temperature, moisture content, pH-value, appearance of fungi in the storage and the concentration of fungal particles in the air. The results show a close connection between particle size, temperature and attack of fungi. During the storage mesophilic and termophilic species of the genera Alternaria, Aspergillus, Cladosporium, Mucor and Penicillium appeared. The concentration of fungal particles is the highest for fine chips and decreases in bigger particles. There was a special focus on the investigation of the properties of coarse chips (G 50), which represent a good compromise between handling, storage losses and health risk due to fungal development. | Frissen kitermelt rövid vágásfordulójú fafajokból előállított apríték tárolása során számos probléma merül fel, melyek eredménye minőség-, szárazanyag- és energiaveszteség. Ebben a tanulmányban a nyersanyag-degradációt befolyásoló tényezők kerültek vizsgálatra, különös tekintettel a kifejlődő gombákra. Ezek rendkívüli elszaporodásának köszönhető a szárazanyag-veszteség mellett a nyersanyag kezelése során fellépő, megnövekedett egészségügyi kockázat is. Nyár faapríték hat hónapos tárolása folyamán az aprítékméret függvényében a következő tényezők mérése zajlott: a tárolón belüli hőmérséklet, nedvességtartalom, pH, a tárolóban megjelenő gombák és azok száma a levegőben. Az eredmények szoros összefüggést mutatnak, különösen az aprítékméret, a hőmérséklet és a gombák száma közt. A tárolás során mezofil és termofil gombák fejlődtek. A következő nemzetségekhez tartozó fajok voltak megfigyelhetők: Alternaria, Aspergillus, Cladosporium, Mucor, Penicillium. A gombák száma a finom aprítéknál volt a legmagasabb, ami az aprítékméret növekedésével csökkent. A kutatás folyamán különös szerepet kapott a középfinom apríték tulajdonságainak vizsgálata (G 50), mely kompromisszumos megoldást jelent a kezelés, tárolási veszteségek és a kifejlődő gombáknak köszönhető egészségügyi kockázatok között

Thermogravimetric and reaction kinetic analysis of biomass samples from an energy plantation

The products of a Hungarian experimental plantation for energy crops were investigated. Young shoots of poplar clones (Populus x euramericana and Populus x interamericana), black locust (Robinia pseudoacacia), willow (Salix alba), and an herbaceous plant (Miscanthus sinensis) revealed unexpectedly similar thermal behavior in inert and oxidative atmospheres, as well. An 8-fold difference in the level of grinding did not result in substantial differences in the thermal decomposition. The effect of oxygen in the ambient gas was studied at low sample masses (0.2-0.4 mg) that excluded the overheating due to the high reaction heat of the combustion process. The presence of oxygen affects the decomposition from ca. 220 degreesC. Nevertheless, the extrapolated onset temperature of the hemicellulose decomposition is practically the same at 0, 5, and 21 V/V% oxygen. A group of 12 experiments, representing two grinding levels, three plant genera and four different heating programs were evaluated simultaneously by the method of least squares employing the model of independent pseudocomponents. All evaluated experiments were well described by the same set of kinetic parameters; only the parameters describing the peak area of the partial processes differed. A technique was recommended for the appropriate handling of the nonrandom errors in the simultaneous evaluation of experiment series

Storage Problems of Poplar Chips from Short Rotation Plantations with Special Emphasis on Fungal Development

There are several problems in storing wood chips freshly harvested from short rotation plantations, which result in quality losses as well as in dry matter and energy losses. The factors influencing the degradation of raw material are examined in this paper with special focus on fungal development. An excessive growth of fungi is connected to dry matter losses and also to an increased health risk during raw material handling.The following factors were measured during 6 months storage of poplar wood chips depending on particle size: box temperature, moisture content, pH-value, appearance of fungi in the storage and the concentration of fungal particles in the air. The results show a close connection between particle size, temperature and attack of fungi. During the storage mesophilic and termophilic species of the genera Alternaria, Aspergillus, Cladosporium, Mucor and Penicillium appeared. The concentration of fungal particles is the highest for fine chips and decreases in bigger particles. There was a special focus on the investigation of the properties of coarse chips (G 50), which represent a good compromise between handling, storage losses and health risk due to fungal development

Effect of Culture Conditions on Fatty Acid Profiles of Bacteria and Lipopolysaccharides of the Genus Pseudomonas—GC-MS Analysis on Ionic Liquid-Based Column

The profiling of bacterial fatty acids is a well-established technique in identifying and classifying bacteria. Cultivation conditions may affect the biosynthesis, thereby, changing the fatty acid profile in bacteria. The effect of the culture conditions on the fatty acid components of Pseudomonas aeruginosa PAO1, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa polyresistant and Pseudomonas putida all are aligned to the genus Pseudomonas. The fatty acids in the lipopolysaccharides of Pseudomonas aeruginosa PAO1 were also examined. The effects of the cultivation conditions were followed by using agar and blood agar media at the characteristic temperatures, 25 °C, 37 °C and 42 °C, respectively, and an analysis was made during the 1st, 3rd and 5th day following inoculation. In addition to quantitative differences, we also experienced qualitative differences in the fatty acid profiles which detect newly appearing fatty acids, due to changes in environmental factors. The application of ionic liquid-based column unveils new possibilities for the analyses of fatty acids in GC-MS experiments for bacterial fatty acid profiling. The validation results (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) show the high separation efficiency of the ionic liquid-based column in the analyses