The comprehensive environmental management model in post-Covid19 era

To restrict the adverse impact of Green House Gas there is a need of rapid action for transformation of the current situation to achieve the targets mentioned by the United Nations Programme. The coronavirus crisis generated additional challenges but also generated a provision for companies to develop a sustainable model in the recovery period by incorporating the non-financial aspect of green environment in their business model. In this paper, we identify some of the main problems of the existing projects related to Green House Gas control and propose the Dragon Value System with a potentiality of overcoming the drawbacks of the existing models. We find a comprehensive environmental management model that will be opportunity driven, based on certain financial assets associated with wide spectrum of stakeholders and applicable by company of any size. The findings of the paper will contribute to the academic literature related to environmental management and SDG 7. The proposed model will assist the investors to identify a comprehensive model to invest and could generate new public-private partnership to apply highly beneficial and comprehensive environmental management model

Humoral and cellular response to BoHV-1 in buffalo and cattle treated with an inactivated marker vaccine

The study is aimed at assessing and comparing the immune response to BoHV-1 elicited by an inactivated marker vaccine in buffaloes and cattle. Vaccination did not produced any local or general reactions in buffaloes. Seroneutralizing antibodies and cellular response by IFN-γ- test have been detected in buffaloes and cattle after a prime/ booster vaccination strategy. Humoral and cellular responses were significantly higher in cattle than in buffaloes. Data pointed out the possibility to use the marker vaccine in buffaloes. However, further studies must be planned to assess the immune pressure of marker vaccines in terms of IBR eradicative attitude in infected buffalo herds

Solar limb brightening observed with SOHO/UVCS

A model of the ultraviolet emission from chromospheric and transition region solar plasma is necessary for a correct interpretation of the measurements of the resonantly scattered spectral lines in solar corona and to determine the stray light correction needed to subtract non-coronal signal from coronagraphic data. That model is useful for analysis of SOHO/UltraViolet Coronagraph Spectrometer (UVCS) observations and for the incoming future missions for coronal study in UV spectral range. Escape probability techniques avoid the need for a full solution of the radiative transfer equation and reduce the question to a geometrical problem. In this paper we use UVCS observations to calculate a simple function to approximate a radial limb brightening

The Comprehensive Environmental Management Model In Post-Covid19 Era

Copyright © the author(s). To restrict the adverse impact of Green House Gas there is a need of rapid action for transformation of the current situation to achieve the targets mentioned by the United Nations Programme. The coronavirus crisis generated additional challenges but also generated a provision for companies to develop a sustainable model in the recovery period by incorporating the non-financial aspect of green environment in their business model. In this paper, we identify some of the main problems of the existing projects related to Green House Gas control and propose the Dragon Value System with a potentiality of overcoming the drawbacks of the existing models. We find a comprehensive environmental management model that will be opportunity driven, based on certain financial assets associated with wide spectrum of stakeholders and applicable by company of any size. The findings of the paper will contribute to the academic literature related to environmental management and SDG 7. The proposed model will assist the investors to identify a comprehensive model to invest and could generate new public-private partnership to apply highly beneficial and comprehensive environmental management model

A multidisciplinary approach to study Sporadic Amyotrophic Lateral Sclerosis in patients with common geographical origin

Amyotrophic Lateral Sclerosis (ALS), a fatal neurodegenerative disorder, is object of intensive research as the causes are still unknown and a treatment not available yet. This project is aimed to study, with a multidisciplinary approach, a small cohort of ALS subjects with a common environmental exposure. For metal quantitation, samples of serum and whole blood were analyzed by ICP-MS. For proteomic analyses, immobilized pH gradient covered the 4-10 and 3-7 pH range. Arsenic concentration resulted significantly lower in patients than in controls. Also, Mn and Hg showed lower levels in patients. Levels of plasma APOA2 protein resulted decreased in patients with respect to controls, whereas SAMP showed a significant decrease only in the late onset group. APOA1 and TTHY also were decreased, the latter in late-onset patients. RET4 was decreased only in the early-onset group. When evaluating APOE genotype we found a 3-fold increase in the frequency of E3/E4 genotype in the patient\u2019s group. DNA oxidative stress has been evaluated through a Comet Assay. The multidisciplinary approach applied in this study allowed to dissect different aspects of ALS, often are evaluated separately and in heterogeneous cohorts of patients

Metal and proteomic analysis of sporadic ALS patients with common geographical origin

Amyotrophic Lateral Sclerosis (ALS) is a rare neurodegenerative disorder characterized by selective degeneration of both upper and lower motor neurons in the brain, brainstem, and spinal cord. This results in paralysis due to muscle weakness and atrophy, leading to death in 3-5 years. Genetic and environmental factors are involved in the pathogenesis of the disease and metals metabolism have been linked to ALS. This study enrolled seven patients and five controls (age matched, living in the same geographical area). For metal quantitation, samples of serum were analyzed by ICP-MS. For proteomic analyses, immobilized pH gradient covered the 4-10 and 3-7 pH range. Statistical analyses were carried out with Student's t-test and Artificial Neural Networks. Among the metals analyzed, As concentration resulted significantly lower in patients than in controls (p=0.007); Hg too was found in lower concentration in patients, but with a lower statistical significance (p=0.13). Higher concentration of Al in patients was detected (p=0.08). In this study, we were not able to confirm the higher concentrations of Ni and Pb in patients previously described in a smaller cohort. Our proteomics data show that APOA2 is decreased by 30% in patients with respect to controls. Furthermore, AHSG and SAP showed a significant decrease in patients with a story of more than 10 years of disease. Impaired metal homeostasis, attributable to environmental exposure, could lead to mineral overload. Besides promoting oxidative stress, metals can compete for the binding sites of metal-containing proteins, such as those containing iron-sulfur clusters. At present, no literature data link APOA2 to ALS, but the fact that its mRNA is processed by TDP43, provides a possible connection with the disease. The proteins differentially expressed belong to the group of Acute Phase Reaction proteins, possibly linking ALS to a chronic inflammation status. Further experiments are still ongoing

BS148 Reduces the Aggressiveness of Metastatic Melanoma via Sigma-2 Receptor Targeting

: The management of advanced-stage melanoma is clinically challenging, mainly because of its resistance to the currently available therapies. Therefore, it is important to develop alternative therapeutic strategies. The sigma-2 receptor (S2R) is overexpressed in proliferating tumor cells and represents a promising vulnerability to target. Indeed, we have recently identified a potent S2R modulator (BS148) that is effective in melanoma. To elucidate its mechanism of action, we designed and synthesized a BS148 fluorescent probe that enters SK-MEL-2 melanoma cells as assessed using confocal microscopy analysis. We show that S2R knockdown significantly reduces the anti-proliferative effect induced by BS148 administration, indicating the engagement of S2R in BS148-mediated cytotoxicity. Interestingly, BS148 treatment showed similar molecular effects to S2R RNA interference-mediated knockdown. We demonstrate that BS148 administration activates the endoplasmic reticulum stress response through the upregulation of protein kinase R-like ER kinase (PERK), activating transcription factor 4 (ATF4) genes, and C/EBP homologous protein (CHOP). Furthermore, we show that BS148 treatment downregulates genes related to the cholesterol pathway and activates the MAPK signaling pathway. Finally, we translate our results into patient-derived xenograft (PDX) cells, proving that BS148 treatment reduces melanoma cell viability and migration. These results demonstrate that BS148 is able to inhibit metastatic melanoma cell proliferation and migration through its interaction with the S2R and confirm its role as a promising target to treat cancer