Regeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells

International audienc

An assessment of genetic diversity within a collection of cassava (Manihot esculenta Crantz) germplasm using molecular markers

Des clones cDNA de Manioc (#Manihot esculenta Crantz) ont été utilisés pour détecter le polymorphisme de longueur des fragments de resriction (RFLP) dans une collection de germplasm de manioc conservée en culture #in vitro au centre ORSTOM de Montpellier. La collection se compose principalement de cultivars africains de #M. esculenta, ainsi que de quelques #M.glaziovii Mueller Von Argau and #M. caerulescens Pohl, et de quelques hybrides interspécifiques de #M. esculenta x #M. glaziovii$. Les sondes cDNA mettent à jour des niveaux de polymorphisme significatifs à la fois à l'intérieur et entre les espèces ; ce qui est suffisant pour créer des dendogrammes indiquant la diversité génétique à l'intérieur de la collection. (Résumé d'auteur

Characterization of the protease domain of Rice tungro bacilliform virus responsible for the processing of the capsid protein from the polyprotein

BACKGROUND: Rice tungro bacilliform virus (RTBV) is a pararetrovirus, and a member of the family Caulimoviridae in the genus Badnavirus. RTBV has a long open reading frame that encodes a large polyprotein (P3). Pararetroviruses show similarities with retroviruses in molecular organization and replication. P3 contains a putative movement protein (MP), the capsid protein (CP), the aspartate protease (PR) and the reverse transcriptase (RT) with a ribonuclease H activity. PR is a member of the cluster of retroviral proteases and serves to proteolytically process P3. Previous work established the N- and C-terminal amino acid sequences of CP and RT, processing of RT by PR, and estimated the molecular mass of PR by western blot assays. RESULTS: A molecular mass of a protein that was associated with virions was determined by in-line HPLC electrospray ionization mass spectral analysis. Comparison with retroviral proteases amino acid sequences allowed the characterization of a putative protease domain in this protein. Structural modelling revealed strong resemblance with retroviral proteases, with overall folds surrounding the active site being well conserved. Expression in E. coli of putative domain was affected by the presence or absence of the active site in the construct. Analysis of processing of CP by PR, using pulse chase labelling experiments, demonstrated that the 37 kDa capsid protein was dependent on the presence of the protease in the constructs. CONCLUSION: The findings suggest the characterization of the RTBV protease domain. Sequence analysis, structural modelling, in vitro expression studies are evidence to consider the putative domain as being the protease domain. Analysis of expression of different peptides corresponding to various domains of P3 suggests a processing of CP by PR. This work clarifies the organization of the RTBV polyprotein, and its processing by the RTBV protease

Analysis of a large number of independent transgenic rice plants produced by the biolistic method

International audienc

Single-mode cavities at frequencies of 172 and 178 MHz

In the report presented here the projects of two accelerating cavities with strong damping of higher modes (HOM) with special vacuum loads are presented. The designs of the cavities and loads are described. The design parameters of cavities, their spectra of higher modes and calculation results of the beam phase motion stability are given for the VEPP-2000 and NANOHANA Projects

Les peroxydases végétales de classe III

International audienc