Comparison of UVB and UVC irradiation disinfection efficacies on Pseudomonas Aeruginosa biofilm

Disinfection routines are important in all clinical applications. The uprising problem of antibiotic resistance has driven major research efforts towards alternative disinfection approaches, involving light-based solutions. Pseudomonasaeruginosa (P. aeruginosa) is a common bacterium that can cause skin, soft tissue, lungs, kidney and urinary tract infections. Moreover, it can be found on and in medical equipment causing often cross infections in hospitals. The objective of this study was to test the efficiency, of two different light-based disinfection treatments, namely UVB and UVC irradiation, on P. aeruginosa biofilms at different growth stages. In our experiments a new type of UV light emitting diodes (LEDs) were used to deliver UV irradiation on the biofilms, in the UVB (296nm) and UVC (266nm) region. The killing rate was studied as a function of dose for 24h grown biofilms. The dose was ramped from 72J/m2 to 10000J/m2. It was shown that UVB irradiation was more effective than UVC irradiation in inactivating P. aeruginosa biofilms. No colony forming units (CFU) were observed for the UVB treated biofilms when the dose was 10000 J/m2 (CFU in control sample: 7.5 x 104). UVB irradiation at a dose of 20000J/m2 on mature biofilms (72h grown) resulted in a 3.9 log killing efficacy. The fact that the wavelength of 296nm exists in daylight and has such disinfection ability on biofilms gives new perspectives for applications within disinfection at hospitals

Thermodynamic basis of the concept of "recombination resistances"

The concept of "recombination resistance" introduced by Shockley and Read (Phys. Rev. 87, 835 (1952)) is discussed within the framework of the thermodynamics of irreversible processes ruled by the principle of the minimum rate of entropy production. It is shown that the affinities of recombination processes represent "voltages" in a thermodynamic Ohm-like law where the net rates of recombinations represent the "currents". The quantities thus found allow for the definition of the "dissipated power" which is to be related to the rate of entropy production of the recombination processes dealt with.Comment: Submitted to Phys. Rev.

Dynamics of the dispersion interaction in an energy transfer system

On the propagation of resonant radiation through an optically dense system, photon capture is commonly followed by one or more near-field transfers of the resulting optical excitation. The process invokes secondary changes to the local electronic environment, shifting the electromagnetic interactions between participant chromophores and producing modified intermolecular forces. From the theory it emerges that energy transfer, when it occurs between chromophores with electronically dissimilar properties, can itself generate significant changes in the intermolecular potentials. This report highlights specific effects that can be anticipated when laser light propagates across an interface between differentially absorbing components in a model energy transfer system

Integration of Catalysis with Storage for the Design of Multi-Electron Photochemistry Devices for Solar Fuel

Decarbonization of the transport system and a transition to a new diversified energy system that is scalable and sustainable, requires a widespread implementation of carbon-neutral fuels. In biomimetic supramolecular nanoreactors for solar-to-fuel conversion, water-splitting catalysts can be coupled to photochemical units to form complex electrochemical nanostructures, based on a systems integration approach and guided by magnetic resonance knowledge of the operating principles of biological photosynthesis, to bridge between long-distance energy transfer on the short time scale of fluorescence, ~10−9 s, and short-distance proton-coupled electron transfer and storage on the much longer time scale of catalysis, ~10−3 s. A modular approach allows for the design of nanostructured optimized topologies with a tunneling bridge for the integration of storage with catalysis and optimization of proton chemical potentials, to mimic proton-coupled electron transfer processes in photosystem II and hydrogenase

Beyond the Yablonovitch limit: trapping light by frequency shift

It is shown that randomising the photon distribution over the frequency as well as orientation variables dramatically improves the efficiency of optical confinement in a weakly absorbing material such as crystalline silicon. The enhancement in average optical path length over the Yablonovitch limit [E. Yablonovitch, J. Opt. Soc. Am. 72, 899 (1982)] is given by an inverse Boltzmann factor of the frequency shift, making it possible to manufacture, for example, efficient crystalline silicon solar cells of thickness barely 1 micromete

Thermodynamics of losses in photovoltaic conversion

This letter presents a thermodynamic analysis of losses in an ideal solar cell. It is shown that the maximum voltage—corresponding to the voltage produced by a hot-carrier solar cell — is equal to the energy of the incident solar photon multiplied by the appropriate Carnot factor. Voltage generated by the usual p-n junction cell is lower on account of entropy generation through kinetic losses, photon cooling, and étendue expansion of the incident beam. Simple expressions can be obtained by an approximation where the energy and entropy changes are modeled by the corresponding expressions for a two-dimensional ideal photon gas