Clinical proteomics experiences in Japan on lung cancer treatments with EGFR -TKI-IRESSA

Comunicaciones a congreso

BioBanking - The Holy Grail of novel drug and diagnostic developments?

The ever increasing social cost that society pays for illness and disease are currently steadily increasing in many countries in the world today. These changes in society becomes a major financial burden that activates politicians and health care organizations in order to find new solutions. Biobanks are becoming the new powerful modality within the field of modern Life Science, that is expected to be important in the proactive awareness of patient health status. Biobanks are also expected to promote the developments of targeted treatments with personalized indicator assays, for effective use of Personalized Medicine treatments in the near future

Results of Five-Year Monitoring of Implantoprosthetic Rehabilitation of Combat Wounds to the Face and Jaws

Udio ozljeda glave u ratnim ozljedama čini gotovo 15%. Strijelne i eksplozivne ozljede specifične su po mehanizmu nastanka i po rezultirajućim oštećenjima. Ratne ozljede maksilofacijalne regije po svojem su opsegu i po opsegu trajnih oštećenja u pravilu mnogo nepovoljnije od mirnodopskih te je tomu proporcionalna i težina rehabilitacije takvih ozljeda. Implantoprotetička rehablitacija danas je rutina, ali je u doba neposredno nakon Domovinskoga rata bila tek u začetcima. U Klinici za kirurgju lica, čeljusti i usta KB “Dubrava” rehabilitirano je više defekata zuba i čeljusti ratnih vojnih i civilnih invalida Domovinskoga rata metodom ugradnje titanskih oseointegrirajućih usadaka, a zatim rehabilitacijom protetskim napravama. Prikazani su slučajevi implantoprotetske rehabilitacije ratnih ozljeda lica i čeljusti s praćenjem više od 5 godina.Of all combat wounds the share of head wounds amounts to almost 15%. Gunshot and explosive wounds are specific both with regard to the mechanism of occurrence and with regard to the resulting damage. Combat wounds in the maxillofacial region, because of their extent and the extent of the permanent damage, are as a rule far worse than those that occur during peacetime, and the difficulty of rehabilitation of such wounds is proportional. Today implantoprosthetic rehabilitation is routine, although in the period immediately after the Homeland War it was just in its initial stage. In the Clinic of Oral and Maxillofacial Surgery University Hospital “Dubrava”, Zagreb several defects of the teeth and jaws in military and civil invalids of war were rehabilitated by the method of inserting titanium osseointegrating implants, followed by rehabilitation with prosthetic devices. Cases are presented of implantoprosthetic rehabilitation of combat wounds to the face and jaws with five-year monitoring

Moving towards high density clinical signature studies with a human proteome catalogue developing multiplexing mass spectrometry assay panels

A perspective overview is given describing the current development of multiplex mass spectrometry assay technology platforms utilized for high throughput clinical sample analysis. The development of targeted therapies with novel personalized medicine drugs will require new tools for monitoring efficacy and outcome that will rely on both the quantification of disease progression related biomarkers as well as the measurement of disease specific pathway/signaling proteins

2DDB – a bioinformatics solution for analysis of quantitative proteomics data

BACKGROUND: We present 2DDB, a bioinformatics solution for storage, integration and analysis of quantitative proteomics data. As the data complexity and the rate with which it is produced increases in the proteomics field, the need for flexible analysis software increases. RESULTS: 2DDB is based on a core data model describing fundamentals such as experiment description and identified proteins. The extended data models are built on top of the core data model to capture more specific aspects of the data. A number of public databases and bioinformatical tools have been integrated giving the user access to large amounts of relevant data. A statistical and graphical package, R, is used for statistical and graphical analysis. The current implementation handles quantitative data from 2D gel electrophoresis and multidimensional liquid chromatography/mass spectrometry experiments. CONCLUSION: The software has successfully been employed in a number of projects ranging from quantitative liquid-chromatography-mass spectrometry based analysis of transforming growth factor-beta stimulated fi-broblasts to 2D gel electrophoresis/mass spectrometry analysis of biopsies from human cervix. The software is available for download at SourceForge

Biobank resources for future patient care: developments, principles and concepts

The aim of the overview is to give a perspective of global biobank development is given in a view of positioning biobanking as a key resource for healthcare to identify new potential markers that can be used in patient diagnosis and complement the targeted personalized drug treatment. The fast progression of biobanks around the world is becoming an important resource for society where the patient benefit is in the focus, with a high degree of personal integrity and ethical standard. Biobanks are providing patient benefits by large scale screening studies, generating large database repositories. It is envisioned by all participating stakeholders that the biobank initiatives will become the future gateway to discover new frontiers within life science and patient care. There is a great importance of biobank establishment globally, as biobanks has been identified as a key area for development in order to speed up the discovery and development of new drugs and protein biomarker diagnostics. One of the major objectives in Europe is to establish concerted actions, where biobank networks are being developed in order to combine and have the opportunity to share and build new science and understanding from complex disease biology. These networks are currently building bridges to facilitate the establishments of best practice and standardizations

Localization of tamoxifen in human breast cancer tumors by MALDI mass spectrometry imaging

Background: Tamoxifen is used in endocrine treatment of breast cancer to inhibit estrogen signaling. A set of strati‐ ed ER‐positive and ER‐negative tumor sections was subjected to manual deposition of tamoxifen solution in order to investigate its spatial distribution upon exposure to interaction within thin tissue sections. Methods: The localization of tamoxifen in tumor sections was assessed by matrix assisted laser deposition/ioniza‐ tion mass spectrometry imaging. The images of extracted ion maps were analyzed for comparison of signal intensity distributions. Results: The precursor ion of tamoxifen (m/z 372.233) displayed heterogeneous signal intensity distributions in his‐ tological compartments of tumor tissue sections. The levels of tamoxifen in tumor cells compared with stroma were higher in ER‐positive tissues, whereas ER‐negative tissue sections showed lower signal intensities in tumor cells. Conclusions: The experimental model was successfully applied on frozen tumor samples allowing for di erentiation between ER groups based on distribution of tamoxifen

Cancer Phenotype Diagnosis and Drug Efficacy within Japanese Health Care

An overview on targeted personalized medicine is given describing the developments in Japan of lung cancer patients. These new targeted therapies with novel personalized medicine drugs require new implementations, in order to follow and monitor drug efficacy and outcome. Examples from IRESSA (Gefitinib) and TARCEVA (Erlotinib) treatments used in medication of lung cancer patients are presented. Lung cancer is one of the most common causes of cancer mortality in the world. The importance of both the quantification of disease progression, where diagnostic-related biomarkers are being implemented, in addition to the actual measurement of disease-specific mechanisms relating to pathway signalling activation of disease-progressive protein targets is summarised. An outline is also presented, describing changes and adaptations in Japan, meeting the rising costs and challenges. Today, urgent implementation of programs to address these needs has led to a rebuilding of the entire approach of medical evaluation and clinical care