New York state's merchandise export gap

New York's merchandise export performance has trailed the nation's for several years. The cause of this gap is not easy to identify: the state maintains a relatively healthy mix of customer markets, remains well represented in industries with strong foreign demand, and continues to enjoy declining labor costs. A broader look at New York's competitiveness, however, reveals that high nonlabor costs may be hurting the state's manufacturing sector and thus its volume of exports.Exports ; Manufactures ; New York (State)

Regulation of phenylacetic acid uptake is σ54 dependent in Pseudomonas putida CA-3

<p>Abstract</p> <p>Background</p> <p>Styrene is a toxic and potentially carcinogenic alkenylbenzene used extensively in the polymer processing industry. Significant quantities of contaminated liquid waste are generated annually as a consequence. However, styrene is not a true xenobiotic and microbial pathways for its aerobic assimilation, via an intermediate, phenylacetic acid, have been identified in a diverse range of environmental isolates. The potential for microbial bioremediation of styrene waste has received considerable research attention over the last number of years. As a result the structure, organisation and encoded function of the genes responsible for styrene and phenylacetic acid sensing, uptake and catabolism have been elucidated. However, a limited understanding persists in relation to host specific regulatory molecules which may impart additional control over these pathways. In this study the styrene degrader <it>Pseudomonas putida </it>CA-3 was subjected to random mini-Tn<it>5 </it>mutagenesis and mutants screened for altered styrene/phenylacetic acid utilisation profiles potentially linked to non-catabolon encoded regulatory influences.</p> <p>Results</p> <p>One mutant, D7, capable of growth on styrene, but not on phenylacetic acid, harboured a Tn<it>5 </it>insertion in the <it>rpoN </it>gene encoding σ54. Complementation of the D7 mutant with the wild type <it>rpoN </it>gene restored the ability of this strain to utilise phenylacetic acid as a sole carbon source. Subsequent RT-PCR analyses revealed that a phenylacetate permease, PaaL, was expressed in wild type <it>P. putida </it>CA-3 cells utilising styrene or phenylacetic acid, but could not be detected in the disrupted D7 mutant. Expression of plasmid borne <it>paaL </it>in mutant D7 was found to fully restore the phenylacetic acid utilisation capacity of the strain to wild type levels. Bioinformatic analysis of the <it>paaL </it>promoter from <it>P. putida </it>CA-3 revealed two σ⁵⁴consensus binding sites in a non-archetypal configuration, with the transcriptional start site being resolved by primer extension analysis. Comparative analyses of genomes encoding phenylacetyl CoA, (PACoA), catabolic operons identified a common association among styrene degradation linked PACoA catabolons in <it>Pseudomonas </it>species studied to date.</p> <p>Conclusions</p> <p>In summary, this is the first study to report RpoN dependent transcriptional activation of the PACoA catabolon <it>paaL </it>gene, encoding a transport protein essential for phenylacetic acid utilisation in <it>P. putida </it>CA-3. Bioinformatic analysis is provided to suggest this regulatory link may be common among styrene degrading <it>Pseudomonads</it>.</p

Self-Presentational Determinants of Sex Differences in Leadership Behavior

Men and women placed in leadership positions communicated information about their skills and abilities to their subordinates. Although leaders’ perceptions of their abilities, group members’ knowledge of their leader’s abilities, and the specific skills needed by the leader were all manipulated in the experimental setting, self-presentations of ability were primarily determined by sex role stereotypes rather than by situational factors. Results indicated that (1) male leaders emphasized their social influence and task abilities; (2) female leaders emphasized their interpersonal, socioemotional abilities; and (3) group members felt task ability, as compared to interpersonal ability, was a far more important skill for a leader to possess. It was concluded that sex differences in male and female leadership behavior may be due to self-presentational conformity to sex roles, and that this conformity enhances males’ leadership effectiveness while detracting from females’ leadership effectiveness

Epidermal Keratinocyte Self-Renewal Is Dependent upon Dermal Integrity

The epidermis is a major site of self-renewal in which there is constant replacement by cell division in the basal layers of cells lost by desquamation in the superficial layers. such a tissue is therefore likely to contain stem cells and in this study we have examined the role of the dermis in the maintenance of epidermal self-renewal. We have developed a mouse model to address the question of whether the maintenance of epidermal self-renewal is dependent, as in the hemopoietic system, upon a heterologous cell type. Intact epidermis separated from disaggregated epidermal cells, can reconstitute a stratified squamous epithelium when grafted onto the lumbo-dermal fascia of the mouse or onto an experimentally induced granulation tissue bed. However, we have shown that, after grafting the clonogenic capacity of the keratinocytes declines sharply and the colonies that are produced are incapable of self-renewal in vitro. Although initially hyperplastic, these epidermal grafts assume an atrophic appearance after 40–70 d this may be related to the loss of self-renewal observed in vitro. With both experimental murine grafts and clinical grafts the failure of keratinocytes to self-renew can be alleviated, partially, by the presence of the dermis in full-thickness or split-thickness grafts, which implies that the dermis has a functional role in epidermal role in epidermal stem cell maintenance. The relevance of these observations to the clinical experience with cultured autologous keratinocyte sheets as would dressings to patients is discussed

A Foldable, Compact and Lightweight Solar Array Substrate with Large Deployed Wingspan for Small Spacecraft

A solar array (SA) mechanical subsystem made of thin and lightweight substrates was developed, built and tested for a small spacecraft. The SA is compactly foldable and deployable to a length of approximately five times the widthof the spacecraft. It has miniature hinges and latches, and deploys freely without dampers and synchronizing mechanisms. The solar cell interconnect harness consists ofthin, laminatedflexible circuits,and the substrates feature a syntactic foam core exposed to large temperature extremes. This developmental technology, currently at TRL 6, when completely proven out, would be viable for small satellites and would enable missions in the Express-class. The Express-class (or Express) refers to satellites in the range of 25kg to 100 kg that are positioned in the gap between 12U CubeSats and small ESPA-class spacecraft. Cornerstones of the SA development were compact packaging, deployment dynamic simulation, and hinge-latch tuning for dynamics and lock-up loads. Dynamic deployment simulations were modeled in Adams to observe the behavior of the unfolding array, to size the hinge springs and to monitor the lockup loads at the substrate to hinge interfaces. Extensive substrate mechanical and thermal tests were conducted to verify the substrate’s structural capability and dimensional stability in its operating environment. Thermal tests were carried out to observe the effect of mismatching coefficients of thermal expansion between the adhered flexible laminated interconnect circuits and the substrate. Gravity-negated wing deployment tests were performed at temperature limits and in vacuum to verify the overall design intent of the deployment. The stowed wing was vibration tested to verify its structural capabilities under launch environments, and then deployment tested again to demonstrate that the array as a mechanism was unaffected by launch loads. Mechanically, the Express SA substrate assembly has been advanced in its development and proven out as a structure and mechanism. Further development of the electrical power system is necessary, and additional testing for mechanical and thermal interactions of the solar cells with the overall SA substrate will need to be done. This SA subsystem would be an essential expansion to the Express hardware developed by The Applied Physics Laboratory (APL) for the advancement and enablement of Express-class missions

Teasing, rejection, and violence: case studies of the school shootings.

Media commentators have suggested that recent school shootings were precipitated by social rejection, but no empirical research has examined this claim. Case studies were conducted of 15 school shootings between 1995 and 2001 to examine the possible role of social rejection in school violence. Acute or chronic rejection-in the form of ostracism, bullying, and/or romantic rejection-was present in all but two of the incidents. In addition, the shooters tended to be characterized by one or more of three other risk factors-an interest in firearms or bombs, a fascination with death or Satanism, or psychological problems involving depression, impulse control, or sadistic tendencies. Implications for understanding and preventing school violence are discussed. Aggr

Oxygen Glucose Deprivation in Rat Hippocampal Slice Cultures Results in Alterations in Carnitine Homeostasis and Mitochondrial Dysfunction

Mitochondrial dysfunction characterized by depolarization of mitochondrial membranes and the initiation of mitochondrial-mediated apoptosis are pathological responses to hypoxia-ischemia (HI) in the neonatal brain. Carnitine metabolism directly supports mitochondrial metabolism by shuttling long chain fatty acids across the inner mitochondrial membrane for beta-oxidation. Our previous studies have shown that HI disrupts carnitine homeostasis in neonatal rats and that L-carnitine can be neuroprotective. Thus, this study was undertaken to elucidate the molecular mechanisms by which HI alters carnitine metabolism and to begin to elucidate the mechanism underlying the neuroprotective effect of L-carnitine (LCAR) supplementation. Utilizing neonatal rat hippocampal slice cultures we found that oxygen glucose deprivation (OGD) decreased the levels of free carnitines (FC) and increased the acylcarnitine (AC): FC ratio. These changes in carnitine homeostasis correlated with decreases in the protein levels of carnitine palmitoyl transferase (CPT) 1 and 2. LCAR supplementation prevented the decrease in CPT1 and CPT2, enhanced both FC and the AC: FC ratio and increased slice culture metabolic viability, the mitochondrial membrane potential prior to OGD and prevented the subsequent loss of neurons during later stages of reperfusion through a reduction in apoptotic cell death. Finally, we found that LCAR supplementation preserved the structural integrity and synaptic transmission within the hippocampus after OGD. Thus, we conclude that LCAR supplementation preserves the key enzymes responsible for maintaining carnitine homeostasis and preserves both cell viability and synaptic transmission after OGD