The bldC developmental locus of Streptomyces coelicolor encodes a member of a family of small DNA-binding proteins related to the DNA-binding domains of the MerR family.

The bldC locus, required for formation of aerial hyphae in Streptomyces coelicolor, was localized by map-based cloning to the overlap between cosmids D17 and D25 of a minimal ordered library. Subcloning and sequencing showed that bldC encodes a member of a previously unrecognized family of small (58- to 78-residue) DNA-binding proteins, related to the DNA-binding domains of the MerR family of transcriptional activators. BldC family members are found in a wide range of gram-positive and gram-negative bacteria. Constructed {Delta}bldC mutants were defective in differentiation and antibiotic production. They failed to form an aerial mycelium on minimal medium and showed severe delays in aerial mycelium formation on rich medium. In addition, they failed to produce the polyketide antibiotic actinorhodin, and bldC was shown to be required for normal and sustained transcription of the pathway-specific activator gene actII-orf4. Although {Delta}bldC mutants produced the tripyrrole antibiotic undecylprodigiosin, transcripts of the pathway-specific activator gene (redD) were reduced to almost undetectable levels after 48 h in the bldC mutant, in contrast to the bldC+ parent strain in which redD transcription continued during aerial mycelium formation and sporulation. This suggests that bldC may be required for maintenance of redD transcription during differentiation. bldC is expressed from a single promoter. S1 nuclease protection assays and immunoblotting showed that bldC is constitutively expressed and that transcription of bldC does not depend on any of the other known bld genes. The bldC18 mutation that originally defined the locus causes a Y49C substitution that results in instability of the protein

Gas and Stellar Motions and Observational Signatures of Co-Rotating Spiral Arms

We have observed a snapshot of our N-body/Smoothed Particle Hydrodynamics simulation of a Milky Way-sized barred spiral galaxy in a similar way to how we can observe the Milky Way. The simulated galaxy shows a co-rotating spiral arm, i.e. the spiral arm rotates with the same speed as the circular speed. We observed the rotation and radial velocities of the gas and stars as a function of the distance from our assumed location of the observer at the three lines of sight on the disc plane, (l, b) = (90, 0), (120, 0) and (150,0) deg. We find that the stars tend to rotate slower (faster) behind (at the front of) the spiral arm and move outward (inward), because of the radial migration. However, because of their epicycle motion, we see a variation of rotation and radial velocities around the spiral arm. On the other hand, the cold gas component shows a clearer trend of rotating slower (faster) and moving outward (inward) behind (at the front of) the spiral arm, because of the radial migration. We have compared the results with the velocity of the maser sources from Reid et al. (2014), and find that the observational data show a similar trend in the rotation velocity around the expected position of the spiral arm at l = 120 deg. We also compared the distribution of the radial velocity from the local standard of the rest, V_LSR, with the APOGEE data at l = 90 deg as an example.Comment: 10 pages, 7 figures, accepted for publication in MNRA

The stellar kinematics of co-rotating spiral arms in Gaia mock observations

We have observed an N-body/Smoothed Particle Hydrodynamics simulation of a Milky Way like barred spiral galaxy. We present a simple method that samples N-body model particles into mock Gaia stellar observations and takes into account stellar populations, dust extinction and Gaia's science performance estimates. We examine the kinematics around a nearby spiral arm at a similar position to the Perseus arm at three lines of sight in the disc plane; (l,b)=(90,0), (120,0) and (150,0) degrees. We find that the structure of the peculiar kinematics around the co-rotating spiral arm, which is found in Kawata et al. (2014b), is still visible in the observational data expected to be produced by Gaia despite the dust extinction and expected observational errors of Gaia. These observable kinematic signatures will enable testing whether the Perseus arm of the Milky Way is similar to the co-rotating spiral arms commonly seen in N-body simulations.Comment: 9 pages 4 Figures, submitted to MNRAS 22nd Dec 201

CydDC-mediated reductant export in Escherichia coli controls the transcriptional wiring of energy metabolism and combats nitrosative stress

The glutathione/cysteine exporter CydDC maintains redox balance in Escherichia coli. A cydD mutant strain was used to probe the influence of CydDC upon reduced thiol export, gene expression, metabolic perturbations, intracellular pH homeostasis, and tolerance to nitric oxide (NO). Loss of CydDC was found to decrease extracytoplasmic thiol levels, whereas overexpression diminished the cytoplasmic thiol content. Transcriptomic analysis revealed a dramatic up-regulation of protein chaperones, protein degradation (via phenylpropionate/phenylacetate catabolism), ?-oxidation of fatty acids, and genes involved in nitrate/nitrite reduction. 1H NMR metabolomics revealed elevated methionine and betaine and diminished acetate and NAD+ in cydD cells, which was consistent with the transcriptomics-based metabolic model. The growth rate and ?pH, however, were unaffected, although the cydD strain did exhibit sensitivity to the NO-releasing compound NOC-12. These observations are consistent with the hypothesis that the loss of CydDC-mediated reductant export promotes protein misfolding, adaptations to energy metabolism, and sensitivity to NO. The addition of both glutathione and cysteine to the medium was found to complement the loss of bd -type cytochrome synthesis in a cydD strain (a key component of the pleiotropic cydDC phenotype), providing the first direct evidence that CydDC substrates are able to restore the correct assembly of this respiratory oxidase. These data provide an insight into the metabolic flexibility of E. coli , highlight the importance of bacterial redox homeostasis during nitrosative stress, and report for the first time the ability of periplasmic low molecular weight thiols to restore haem incorporation into a cytochrome complex

Radial Distribution of Stellar Motions in Gaia DR2

By taking advantage of the superb measurements of position and velocity for an unprecedented large number of stars provided in Gaia DR2, we have generated the first maps of the rotation velocity, $V_{\rm rot}$, and vertical velocity, $V_{\rm z}$, distributions as a function of the Galactocentric radius, $R_{\rm gal}$, across a radial range of $5<R_{\rm gal}<12$~kpc. In the $R-V_{\rm rot}$ map, we have identified many diagonal ridge features, which are compared with the location of the spiral arms and the expected outer Lindblad resonance of the Galactic bar. We have detected also radial wave-like oscillations of the peak of the vertical velocity distribution.Comment: 5 pages, 3 figures, accepted for publication in MNRAS Lette

Spiral and bar driven peculiar velocities in Milky Way sized galaxy simulations

We investigate the kinematic signatures induced by spiral and bar structure in a set of simulations of Milky Way-sized spiral disc galaxies. The set includes test particle simulations that follow a quasi-stationary density wave-like scenario with rigidly rotating spiral arms, and $N$-body simulations that host a bar and transient, co-rotating spiral arms. From a location similar to that of the Sun, we calculate the radial, tangential and line-of-sight peculiar velocity fields of a patch of the disc and quantify the fluctuations by computing the power spectrum from a two-dimensional Fourier transform. We find that the peculiar velocity power spectrum of the simulation with a bar and transient, co-rotating spiral arms fits very well to that of APOGEE red clump star data, while the quasi-stationary density wave spiral model without a bar does not. We determine that the power spectrum is sensitive to the number of spiral arms, spiral arm pitch angle and position with respect to the spiral arm. However, it is necessary to go beyond the line of sight velocity field in order to distinguish fully between the various spiral models with this method. We compute the power spectrum for different regions of the spiral discs, and discuss the application of this analysis technique to external galaxies.Comment: 14 pages, 11 figures. Improved and MNRAS Accepte

A geochemical study of the winonaites: Evidence for limited partial melting and constraints on the precursor composition

The winonaites are primitive achondrites which are associated with the IAB iron meteorites. Textural evidence implies heating to at least the Fe, Ni–FeS cotectic, but previous geochemical studies are ambiguous about the extent of silicate melting in these samples. Oxygen isotope evidence indicates that the precursor material may be related to the carbonaceous chondrites. Here we analysed a suite of winonaites for modal mineralogy and bulk major- and trace-element chemistry in order to assess the extent of thermal processing as well as constrain the precursor composition of the winonaite-IAB parent asteroid. Modal mineralogy and geochemical data are presented for eight winonaites. Textural analysis reveals that, for our sub-set of samples, all except the most primitive winonaite (Northwest Africa 1463) reached the Fe, Ni–FeS cotectic. However, only one (Tierra Blanca) shows geochemical evidence for silicate melting processes. Tierra Blanca is interpreted as a residue of small-degree silicate melting. Our sample of Winona shows geochemical evidence for extensive terrestrial weathering. All other winonaites studied here (Fortuna, Queen Alexander Range 94535, Hammadah al Hamra 193, Pontlyfni and NWA 1463) have chondritic major-element ratios and flat CI-normalised bulk rare-earth element patterns, suggesting that most of the winonaites did not reach the silicate melting temperature. The majority of winonaites were therefore heated to a narrow temperature range of between ∼1220 (the Fe, Ni–FeS cotectic temperature) and ∼1370 K (the basaltic partial melting temperature). Silicate inclusions in the IAB irons demonstrate partial melting did occur in some parts of the parent body (Ruzicka and Hutson, 2010), thereby implying heterogeneous heat distribution within this asteroid. Together, this indicates that melting was the result of internal heating by short-lived radionuclides. The brecciated nature of the winonaites suggests that the parent body was later disrupted by a catastrophic impact, which allowed the preservation of the largely unmelted winonaites. Despite major-element similarities to both ordinary and enstatite chondrites, trace-element analysis suggests the winonaite parent body had a carbonaceous chondrite-like precursor composition. The parent body of the winonaites was volatile-depleted relative to CI, but enriched compared to the other carbonaceous classes. The closest match are the CM chondrites; however, the specific precursor is not sampled in current meteorite collections

Ascorbic acid oxidation: A potential cause of the elevated severity of atherosclerosis in diabetes mellitus?

AbstractThe exposure of mouse peritoneal macrophages to cholesterol linoleate-containing, artificial lipoproteins can lead to intracellular ceroid accumulation. This can be used as a model to study the role of oxidation in macrophage uptake of lipoproteins containing unsaturated fatty acids, considered by many as a primary event in atherosclerotic plaque formation. Our studies show that ascorbic acid can both inhibit and promote the formation of ceroid in such a model system. The transition metal copper (Cu(II)) further elevates ceroid accumulation and EDTA, a metal chelator, inhibits it. When trace levels of transition metals are present, low concentrations of ascorbic acid can elevate ceroid formation. This pro- and antioxidant characteristic of ascorbic acid was confirmed by monitoring the generation of oxidants by various concentrations of ascorbic acid, assessed by benzoic acid hydroxylation or the fragmentation of BSA. We discuss these observations in the context of an apparent increase in ascorbic acid oxidation and elevated severity of atherosclerosis in diabetes mellitus

Population-Dynamics of Juvenile Caribbean Spiny Lobster, Panulirus-Argus, in Florida Bay, Florida

Despite a wealth of information on the growth and population dynamics of sub-adult and adult Caribbean spiny lobsters (Panulirus argus), there is far less information about younger juveniles under natural conditions. Here we describe growth and population dynamics of juvenile spiny lobsters (12-68 mm carapace length, CL) that we have studied for 14 months (October 1988-December 1989) using mark-recapture techniques in a hardbottom community in Florida Bay, Florida. We also monitored the supply of postlarvae into the region in 1988 and 1989 using Witham-type surface collectors in an effort to link peak periods of settlement of postlarvae with subsequent cohorts of juveniles. Field estimates of growth were the highest ever reported for this species, averaging 0.95 mm CL.wk-1 (range: 0.35-1.25 mm CL.wk-1 for individuals 20-25 mm CL and 40-45 mm CL, respectively). These results indicate that lobsters in some areas in Florida Bay can reach Florida\u27s legal harvestable size (76 mm CL) 1.5 years after settlement. Season and lobster size had significant effects on growth rates; slower growth occurred during the winter and among small individuals. Differences in growth among size classes resulted from changes in molt increment, whereas seasonal differences were a result of changes in intermolt interval. Using mark-recapture techniques, we estimate that the density of juvenile spiny lobsters \u3c45 mm CL in this prime nursery habitat was 454.ha-1, that the mean monthly probability of survival (reflecting actual mortality plus emigration) was 0.51, and that an average of 131 lobsters entered the population through recruitment and immigration each month. Recruitment of juveniles was significantly correlated (r = 0.83) with the supply of postlarvae to the region 8 months earlier. This relationship is stronger than was previously believed, and may only be manifested in areas with superior nursery habitat

Factors Affecting the Recruitment of Juvenile Caribbean Spiny Lobsters Dwelling in Macroalgae

In south Florida, Caribbean spiny lobsters (Panulirus argus) settle and spend their first few months in macroalgae or seagrass. After a few months, these \u27\u27algal-phase\u27\u27 juveniles emerge from vegetation and, as \u27\u27postalgal-phase\u27\u27 juveniles, seek refuge in crevices, often dwelling in groups. The importance of crevice shelters in determining the abundance of postalgal-phase juvenile spiny lobsters has been studied but we know little about the processes affecting lobster distribution and survival during their cryptic algal-dwelling phase. We found that postlarval supply varied independently of changes in the structure of macroalgal settlement habitat. For this reason, postlarval supply alone can not reliably predict local settlement density. Changes in the size of macroalgal patches in particular tend to increase the variability in settlement density among locations and times. Field and mesocosm experiments indicate that social interactions and individual movements are unlikely to alter the general distribution of algal-phase lobsters established at settlement. But if algal-phase lobsters are aggregated at scales \u3c1 \u3em(2) (e.g., due to patchy settlement), they experience higher mortality than non-aggregated lobsters, as revealed in field experiments where lobsters were tethered alone or in pairs and at varying inter-individual distances. Field manipulations of settlement density indicate that recapture (survival) of microwire tagged algal-phase juveniles is positively associated with features of the habitat that affect lobster density (e.g., site area, macroalgal patch size), but survival and growth of lobsters are unrelated to artificially manipulated settlement density. Collectively, these results imply that the population dynamics of juvenile P. argus dwelling in macroalgae are not typically regulated by density-dependent processes, although density-dependent predation may be locally important in patches when settlement is episodically high