Myosin ii regulatory light chain phosphorylation and formin availability modulate cytokinesis upon changes in carbohydrate metabolism.

Cytokinesis, the separation of daughter cells at the end of mitosis, relies in animal cells on a contractile actomyosin ring (CAR) composed of actin and class II myosins, whose activity is strongly influenced by regulatory light chain (RLC) phosphorylation. However, in simple eukaryotes such as the fission yeast Schizosaccharomyces pombe, RLC phosphorylation appears dispensable for regulating CAR dynamics. We found that redundant phosphorylation at Ser35 of the S. pombe RLC homolog Rlc1 by the p21- activated kinases Pak1 and Pak2, modulates myosin II Myo2 activity and becomes essential for cytokinesis and cell growth during respiration. Previously, we showed that the stress activated protein kinase pathway (SAPK) MAPK Sty1 controls fission yeast CAR integrity by downregulating formin For3 levels (Gomez-Gil et al.,2020). Here, we report that the reduced availability of formin For3-nucleated actin filaments for the CAR is the main reason for the required control of myosin II contractile activity by RLC phosphorylation during respiration-induced oxidative stress. Thus, the restoration of For3 levels by antioxidants overrides the control of myosin II function regulated by RLC phosphorylation, allowing cytokinesis and cell proliferation during respiration. Therefore, fine-tuned interplay between myosin II function through Rlc1 phosphorylation and environmentally controlled actin filament availability is critical for a successful cytokinesis in response to a switch to a respiratory carbohydrate metabolism.Agencia Estatal de Investigación PID2020-112569GB-I00 José Cansado Agencia Estatal de Investigación PGC2018-098924-B-I00 Pilar Pérez Regional Government of Castile and Leon CSI150P20 Pilar Pérez The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication

Diálogos em rede

Excertos das entrevistas realizadas, no âmbito do projecto unplace, a artistas, curadores e investigadores = Excerpts from the interviews carried out, in the scope of the project unplace, with artists, curators and researchers

Plan Andaluz de Cuidados Paliativos, 2008-2012

Publicado en la página web de la Consejería de Igualdad, Salud y Políticas Sociales: www.juntadeandalucia.es/salud (Consejería de Igualdad, Salud y Políticas Sociales / Ciudadanía / Quiénes somos / Planes y Estrategias)YesEste plan nace con el objetivo de proporcionar una atención integral que dé una respuesta adecuada a las necesidades de diversa índole -físicas, emocionales, sociales y espirituales,.- que presentan las personas que se hallan en una situación terminal, así como a sus familiares, procurándoles el máximo grado de bienestar y calidad de vida posibles, siempre dentro del respeto a la dignidad y voluntad personal en el que es su último periodo vita

Incidence, Clinical Characteristics, and Management of Psoriasis Induced by Anti-TNF Therapy in Patients with Inflammatory Bowel Disease: A Nationwide Cohort Study.

Psoriasis induced by anti-tumor necrosis factor-α (TNF) therapy has been described as a paradoxical side effect. To determine the incidence, clinical characteristics, and management of psoriasis induced by anti-TNF therapy in a large nationwide cohort of inflammatory bowel disease patients. Patients with inflammatory bowel disease were identified from the Spanish prospectively maintained Estudio Nacional en Enfermedad Inflamatoria Intestinal sobre Determinantes genéticos y Ambientales registry of Grupo Español de Trabajo en Enfermedad de Croh y Colitis Ulcerosa. Patients who developed psoriasis by anti-TNF drugs were the cases, whereas patients treated with anti-TNFs without psoriasis were controls. Cox regression analysis was performed to identify predictive factors. Anti-TNF-induced psoriasis was reported in 125 of 7415 patients treated with anti-TNFs (1.7%; 95% CI, 1.4-2). The incidence rate of psoriasis is 0.5% (95% CI, 0.4-0.6) per patient-year. In the multivariate analysis, the female sex (HR 1.9; 95% CI, 1.3-2.9) and being a smoker/former smoker (HR 2.1; 95% CI, 1.4-3.3) were associated with an increased risk of psoriasis. The age at start of anti-TNF therapy, type of inflammatory bowel disease, Montreal Classification, and first anti-TNF drug used were not associated with the risk of psoriasis. Topical steroids were the most frequent treatment (70%), achieving clinical response in 78% of patients. Patients switching to another anti-TNF agent resulted in 60% presenting recurrence of psoriasis. In 45 patients (37%), the anti-TNF therapy had to be definitely withdrawn. The incidence rate of psoriasis induced by anti-TNF therapy is higher in women and in smokers/former smokers. In most patients, skin lesions were controlled with topical steroids. More than half of patients switching to another anti-TNF agent had recurrence of psoriasis. In most patients, the anti-TNF therapy could be maintained

Sars-Cov-2 Infection in Patients on Long-Term Treatment with Macrolides in Spain: A National Cross-Sectional Study

The aim of this study was to know the prevalence and severity of COVID-19 in patients treated with long-term macrolides and to describe the factors associated with worse outcomes. A cross-sectional study was conducted in Primary Care setting. Patients with macrolides dispensed continuously from 1 October 2019 to 31 March 2020, were considered. Main outcome: diagnosis of coronavirus disease-19 (COVID-19). Secondary outcomes: symptoms, severity, characteristics of patients, comorbidities, concomitant treatments. A total of 3057 patients met the inclusion criteria. Median age: 73 (64–81) years; 55% were men; 62% smokers/ex-smokers; 56% obese/overweight. Overall, 95% of patients had chronic respiratory diseases and four comorbidities as a median. Prevalence of COVID-19: 4.8%. This was in accordance with official data during the first wave of the pandemic. The most common symptoms were respiratory: shortness of breath, cough, and pneumonia. Additionally, 53% percent of patients had mild/moderate symptoms, 28% required hospital admission, and 19% died with COVID-19. The percentage of patients hospitalized and deaths were 2.6 and 5.8 times higher, respectively, in the COVID-19 group (p < 0.001). There was no evidence of a beneficial effect of long-term courses of macrolides in preventing SARS-CoV-2 infection or the progression to worse outcomes in old patients with underlying chronic respiratory diseases and a high burden of comorbidity

Provincia de Santiago del Estero

Por primera vez a nivel nacional, se describen en un trabajo las principales buenas prácticas para las distintas ecorregiones y provincias del país. En la presente obra se incluyen aquellas prácticas de manejo del suelo y del agua relevante para el buen uso y conservación del suelo en áreas de secano, es decir, en tierras que solamente se benefician con el agua de lluvia. Se incluyen también las prácticas que consideran el uso de vegetación como base para la conservación del suelo. El manual contiene 25 capítulos redactados por especialistas referentes de las instituciones oficiales y privadas del país, los que en forma generosa han aportado la valiosa información y experticia que permitió plasmar la presente obra. Las prácticas se describen tomando como base el mapa de regionalización ecológica-productiva que los equipos técnicos han propuesto para cada provincia. Las mismas han sido referidas a las climosecuencias del área, los tipos de suelos más representativos, los procesos de degradación identificados y la normativa legal vigente, siguiendo el formato de ficha técnica: i) Nombre, ii) Definición, iii) Objetivo, iv) Condiciones para su aplicación, v) Superficie estimada de aplicación, vi) Normas técnicas, vii) Equipo necesario, y viii) Mantenimiento. En esta obra queda demostrado que existen actualmente en la Argentina los conocimientos y tecnologías disponibles para desarrollar una producción agropecuaria sustentable. Ello asegurará un rol estratégico como país productor de alimentos, con respeto a las normas ambientales y de seguridad agroalimentaria. En un futuro inmediato se constituirán en exigencias del comercio internacional, evaluando el camino de la trazabilidad -huellas de carbono e hídrica- de los diferentes productos agropecuarios y la emisión de gases de efecto invernadero. La conservación del suelo y del agua constituye un deber inexcusable, ya que se trata de recursos naturales estratégicos para la Nación Argentina, que cumplen funciones de alcance social y que trascienden las generaciones. Este capítulo describe la zona de la provincia de Santiago del Estero: La provincia de Santiago del Estero posee una superficie de 145.690 km2 se encuentra ubicada entre los 25° 35´ y los 30° 41´ de latitud sur y entre los 61° 34´ y los 65° 34´ de longitud oeste. Su territorio es una planicie que presenta una pequeña inclinación en dirección noroeste-sureste, interrumpida por los ríos que corren en sentido diagonal y por elevaciones ubicadas en la zona sur, oeste y noroeste; donde se registran las mayores altitudes provinciales, que no superan los 700 m de altura (sierras de Guasayán, Sumampa y Ambargasta).Fil: Sanchez, María C.. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Lopez Rivilli, Marisa Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Duffau, Alejandro R.. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Galizzi, Fernando A.. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Barraza, Gabriela A.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Amarilla, Mabel Elizabeth. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Prieto Angueira, Salvador. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Berton, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Ventura, Javier. Establecimiento Monte Buey Agropecuaria S.R.L.; ArgentinaFil: Lescano, Marcelo D.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Storniolo, Angel del Rosario. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Exactas y Tecnologías. Departamento de Geología y Geotecnia; ArgentinaFil: Terribile, Elsa Marcela. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Exactas y Tecnologías. Departamento de Geología y Geotecnia; ArgentinaFil: Thir, Juan Martin. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Exactas y Tecnologías. Departamento de Geología y Geotecnia; ArgentinaFil: Trejo, Walter Mario. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Exactas y Tecnologías. Departamento de Geología y Geotecnia; ArgentinaFil: Pavón, Julio A.. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Exactas y Tecnologías. Departamento de Geología y Geotecnia; ArgentinaFil: Contreras, Sofia Guadalupe. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Exactas y Tecnologías. Departamento de Geología y Geotecnia; ArgentinaFil: Silberman, Juan Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Albanesi de Garay, Ada Susana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Anriquez, Analia Liliana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Kunst, Carlos Roberto Guillermo. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Domínguez Nuñez, José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; ArgentinaFil: Suarez, Ariel. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Ledesma, Roxana. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Grasso, Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; ArgentinaFil: Navall, Jorge Marcelo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Vizgarra, Lidia Amanda. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Venier, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Estudios Agropecuarios; ArgentinaFil: Kowaljow, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Vaieretti, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ferreras, Ana Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Gómez, Adriana T.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Tamer, Ariel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Puig, Omar. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Coronel, Gustavo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Prieto, Daniel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Angueira, Cristina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Angella, Gabriel Augusto. Instituto Nacional de Tecnología Agropecuaria; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Cornacchione, Monica Viviana. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ledesma, Martín. Empresa Sucesores de Antonio Lladhon SL..R.; ArgentinaFil: Bolañez, Luciana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Mas, Laura Inés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucumán-Santiago del Estero. Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Alvarez, Carina Rosa. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Osinaga, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Salvatierra, José I.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Tomsic, Pablo D.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Lizzi, José. Establecimiento El Mangrullo S.A.; ArgentinaFil: Perez Farhat, Walter. Empresa PASA Fertilizantes; Argentin

COVID-19 in hospitalized HIV-positive and HIV-negative patients : A matched study

CatedresObjectives: We compared the characteristics and clinical outcomes of hospitalized individuals with COVID-19 with [people with HIV (PWH)] and without (non-PWH) HIV co-infection in Spain during the first wave of the pandemic. Methods: This was a retrospective matched cohort study. People with HIV were identified by reviewing clinical records and laboratory registries of 10 922 patients in active-follow-up within the Spanish HIV Research Network (CoRIS) up to 30 June 2020. Each hospitalized PWH was matched with five non-PWH of the same age and sex randomly selected from COVID-19@Spain, a multicentre cohort of 4035 patients hospitalized with confirmed COVID-19. The main outcome was all-cause in-hospital mortality. Results: Forty-five PWH with PCR-confirmed COVID-19 were identified in CoRIS, 21 of whom were hospitalized. A total of 105 age/sex-matched controls were selected from the COVID-19@Spain cohort. The median age in both groups was 53 (Q1-Q3, 46-56) years, and 90.5% were men. In PWH, 19.1% were injecting drug users, 95.2% were on antiretroviral therapy, 94.4% had HIV-RNA < 50 copies/mL, and the median (Q1-Q3) CD4 count was 595 (349-798) cells/μL. No statistically significant differences were found between PWH and non-PWH in number of comorbidities, presenting signs and symptoms, laboratory parameters, radiology findings and severity scores on admission. Corticosteroids were administered to 33.3% and 27.4% of PWH and non-PWH, respectively (P = 0.580). Deaths during admission were documented in two (9.5%) PWH and 12 (11.4%) non-PWH (P = 0.800). Conclusions: Our findings suggest that well-controlled HIV infection does not modify the clinical presentation or worsen clinical outcomes of COVID-19 hospitalization

How do women living with HIV experience menopause? Menopausal symptoms, anxiety and depression according to reproductive age in a multicenter cohort

CatedresBackground: To estimate the prevalence and severity of menopausal symptoms and anxiety/depression and to assess the differences according to menopausal status among women living with HIV aged 45-60 years from the cohort of Spanish HIV/AIDS Research Network (CoRIS). Methods: Women were interviewed by phone between September 2017 and December 2018 to determine whether they had experienced menopausal symptoms and anxiety/depression. The Menopause Rating Scale was used to evaluate the prevalence and severity of symptoms related to menopause in three subscales: somatic, psychologic and urogenital; and the 4-item Patient Health Questionnaire was used for anxiety/depression. Logistic regression models were used to estimate odds ratios (ORs) of association between menopausal status, and other potential risk factors, the presence and severity of somatic, psychological and urogenital symptoms and of anxiety/depression. Results: Of 251 women included, 137 (54.6%) were post-, 70 (27.9%) peri- and 44 (17.5%) pre-menopausal, respectively. Median age of onset menopause was 48 years (IQR 45-50). The proportions of pre-, peri- and post-menopausal women who had experienced any menopausal symptoms were 45.5%, 60.0% and 66.4%, respectively. Both peri- and post-menopause were associated with a higher likelihood of having somatic symptoms (aOR 3.01; 95% CI 1.38-6.55 and 2.63; 1.44-4.81, respectively), while post-menopause increased the likelihood of having psychological (2.16; 1.13-4.14) and urogenital symptoms (2.54; 1.42-4.85). By other hand, post-menopausal women had a statistically significant five-fold increase in the likelihood of presenting severe urogenital symptoms than pre-menopausal women (4.90; 1.74-13.84). No significant differences by menopausal status were found for anxiety/depression. Joint/muscle problems, exhaustion and sleeping disorders were the most commonly reported symptoms among all women. Differences in the prevalences of vaginal dryness (p = 0.002), joint/muscle complaints (p = 0.032), and sweating/flush (p = 0.032) were found among the three groups. Conclusions: Women living with HIV experienced a wide variety of menopausal symptoms, some of them initiated before women had any menstrual irregularity. We found a higher likelihood of somatic symptoms in peri- and post-menopausal women, while a higher likelihood of psychological and urogenital symptoms was found in post-menopausal women. Most somatic symptoms were of low or moderate severity, probably due to the good clinical and immunological situation of these women