Cytotoxicity evaluation of haloperidol, clozapine and a new molecule with antipsychotic potential, PT-31, in NIH-3T3 cells

Schizophrenia is an illness that affects 26 million people worldwide. However, conventional antipsychotics present side effects and toxicity, highlighting the need for new antipsychotics. We aimed to evaluate the cytotoxicity of haloperidol (HAL), clozapine (CLO), and a new molecule with antipsychotic potential, PT-31, in NIH-3T3 cells. The neutral red uptake assay and the MTT assay were performed to evaluate cell viability and mitochondrial activity, morphological changes were assessed, and intracellular reactive oxygen species (ROS) detection was performed. HAL and CLO (0.1 μM) showed a decrease in cell viability in the neutral red uptake assay and in the MTT assay. In addition, cell detachment, content decrease, rounding and cell death were also observed at 0.1 μM for both antipsychotics. An increase in ROS was observed for HAL (0.001, 0.01 and 1 μM) and CLO (0.01 and 1 μM). PT-31 did not alter cell viability in any of the assays, although it increased ROS at 0.01 and 1 μM. HAL and CLO present cytotoxicity at 0.1 μM, possibly through apoptosis and necrosis. In contrast, PT-31 does not present cytotoxicity to NIH-3T3 cells. Further studies must be performed for a better understanding of these mechanisms and the potential risk of conventional antipsychotics

Cytotoxicity evaluation of haloperidol, clozapine and a new molecule with antipsychotic potential, PT-31, in NIH-3T3 cells

Abstract Schizophrenia is an illness that affects 26 million people worldwide. However, conventional antipsychotics present side effects and toxicity, highlighting the need for new antipsychotics. We aimed to evaluate the cytotoxicity of haloperidol (HAL), clozapine (CLO), and a new molecule with antipsychotic potential, PT-31, in NIH-3T3 cells. The neutral red uptake assay and the MTT assay were performed to evaluate cell viability and mitochondrial activity, morphological changes were assessed, and intracellular reactive oxygen species (ROS) detection was performed. HAL and CLO (0.1 μM) showed a decrease in cell viability in the neutral red uptake assay and in the MTT assay. In addition, cell detachment, content decrease, rounding and cell death were also observed at 0.1 μM for both antipsychotics. An increase in ROS was observed for HAL (0.001, 0.01 and 1 μM) and CLO (0.01 and 1 μM). PT-31 did not alter cell viability in any of the assays, although it increased ROS at 0.01 and 1 μM. HAL and CLO present cytotoxicity at 0.1 μM, possibly through apoptosis and necrosis. In contrast, PT-31 does not present cytotoxicity to NIH-3T3 cells. Further studies must be performed for a better understanding of these mechanisms and the potential risk of conventional antipsychotics

Ações extensionistas e o diálogo com as comunidades contemporâneas.

As possibilidades que o tema desta segunda edição da Coleção Extensão e Sociedade oportuniza são muitas, porque o diálogo é a condição essencial da Extensão Universitária, a ponto de ser um princípio determinante. Portanto, quando a chamada foi lançada para que os autores submetessem textos oriundos de ações extensionistas, já se esperava que as submissões desenhassem um quadro de diversidade ampla. A expectativa foi cumprida. O que se poderá observar nesta coletânea é como o princípio do diálogo ocorre em campos diversos. Sobre tal aspecto, há de se observar que para a extensão, o diálogo deve ocorrer entre diferentes grupos. A Universidade é, de modo geral, um grupo e a sociedade, abarca todos os demais. Isto, diga-se logo, sob um ponto de vista amplo, porque quanto mais o olhar se aproxima dos fatos, encontram-se no primeiro grupo, muitos outros, tão diversos entre si quanto o são aqueles que se resolveu reunir em uma categoria imensa: a sociedade. Portanto, os influxos desejáveis no processo dialógico são esperados dentro e entre os grupos e é, possivelmente, a maior energia que uma atividade extensionista possa gerar: a ebulição de ideias (nem sempre convergentes) que o diálogo entre os diversos, internos e externos, acaba gerando no processo de interação. E como não há extensão sem interação, vamos aceitar que quando os dois universos, o acadêmico e a sociedade, aproximam-se, nem sempre é possível saber quais os planetas, de cada dimensão, que orbitarão no processo interativo