Mesoporous silica nanoparticles grafted with a light-responsive protein shell for highly cytotoxic antitumoral therapy

A novel phototriggered drug delivery nanocarrier, which exhibits very high tumor cytotoxicity against human tumoral cells, is presented. This device is based on mesoporous silica nanoparticles decorated with a biocompatible protein shell cleavable by light irradiation. The proteins that compose the protein shell (avidin, streptavidin and biotinylated transferrin) act as targeting and capping agents at the same time, avoiding the use of redundant systems. The light responsive behavior is provided by a biotinylated photocleavable cross-linker covalently grafted on the mesoporous surface, which suffers photocleavage by UV radiation (366 nm). Human tumoral cells incubated in the presence of a very low particle concentration enter into the apoptotic stage after a short irradiation time. Thus, the system described here could be applied to the treatment of exposed tumors that affect the skin, oesophagus, and stomach, among others, and are easily accessible for light irradiation

Automatic processing of number identity and place-value in multi-digit numbers

The Arabic numerical system employs two dimensions to create multi-digit numbers: digits symbols and place-value. The present research explores in two numerical Stroop experiments to which extent number identity and number place-value processing is automatic in four-digit numbers. Whereas the automatic coding of place-value has been stated previously (see Kallai & Tzelgov, 2012), this has been studied in isolation using similar numbers (e.g., 0400-0040). Experiment 1 explored the access to numbers identity. Fifty-five volunteers were presented with pairs of four digit numbers that differed in one number (e.g., 0200-0400). They had to decide which number string was presented in bigger font size. Congruity between the physical size and the numerical value, distance between the numbers, and position of the discrepant numbers within the string, were manipulated. Results revealed congruity effects that were modulated by distance and position. Experiment 2 (N=90) jointly manipulated size congruity at the place-value (PV) and at the number identity (NI) dimensions (e.g., congruent NI & PV: 0100-5000; congruent NI & incongruent PV: 1000-0500; incongruent NI & congruent PV: 0500-1000; incongruent NI & incongruent PV: 5000-0100). Data analyses indicated an interaction between place-value and identity showing the automatic and simultaneous processing of both dimensions in four-digit numbers.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Seminario de Iniciación Profesional como Práctica Profesional Supervisada

Esta ponencia tiene como objetivo, presentar en el “8° Encuentro de Docentes de Asignaturas con contenidos jurídicos en Facultades de Ciencias Económicas”, una propuesta de Seminario elaborada por la cátedra de Organización Profesional, que pueda ser reconocido como una Práctica Profesional Supervisada. En el contexto actual nos encontramos próximos a un proceso de acreditación de la carrera de Contador Público en todas las universidades de nuestro país. El Ministerio de Educación a través de la Resolución 3400-E/2017 ha dado carácter normativo a los contenidos curriculares básicos, la carga horaria mínima, los criterios de intensidad de la formación práctica y los estándares para la acreditación de la carrera correspondiente al título de Contador Público. En consonancia con los estándares, la Facultad de Ciencias Económicas a partir de febrero de este año ha implementado el nuevo plan de estudios de la carrera de Contador Público. Dentro de las modificaciones que tiene el Plan de Estudios VII, vale destacar que se encuentra la incorporación de la Práctica Profesional Supervisada (PPS), como un espacio curricular específico de integración de conocimientos, para jerarquizar la instancia de profesionalización en el proceso de formación. En tal sentido, desde la cátedra proponemos y fundamentamos un Seminario de Iniciación Profesional como PPS, que se basará en técnicas adecuadas para este tipo de aprendizajes como la problematización y los ejercicios de simulación. A lo largo del trabajo se presentan los objetivos generales, la metodología de enseñanza, el temario general y la descripción de las herramientas a utilizar.Facultad de Ciencias Económica

Seminario de Iniciación Profesional como Práctica Profesional Supervisada

Esta ponencia tiene como objetivo, presentar en el “8° Encuentro de Docentes de Asignaturas con contenidos jurídicos en Facultades de Ciencias Económicas”, una propuesta de Seminario elaborada por la cátedra de Organización Profesional, que pueda ser reconocido como una Práctica Profesional Supervisada. En el contexto actual nos encontramos próximos a un proceso de acreditación de la carrera de Contador Público en todas las universidades de nuestro país. El Ministerio de Educación a través de la Resolución 3400-E/2017 ha dado carácter normativo a los contenidos curriculares básicos, la carga horaria mínima, los criterios de intensidad de la formación práctica y los estándares para la acreditación de la carrera correspondiente al título de Contador Público. En consonancia con los estándares, la Facultad de Ciencias Económicas a partir de febrero de este año ha implementado el nuevo plan de estudios de la carrera de Contador Público. Dentro de las modificaciones que tiene el Plan de Estudios VII, vale destacar que se encuentra la incorporación de la Práctica Profesional Supervisada (PPS), como un espacio curricular específico de integración de conocimientos, para jerarquizar la instancia de profesionalización en el proceso de formación. En tal sentido, desde la cátedra proponemos y fundamentamos un Seminario de Iniciación Profesional como PPS, que se basará en técnicas adecuadas para este tipo de aprendizajes como la problematización y los ejercicios de simulación. A lo largo del trabajo se presentan los objetivos generales, la metodología de enseñanza, el temario general y la descripción de las herramientas a utilizar.Facultad de Ciencias Económica

Detection and molecular characterization of porcine parvovirus in fetal tissues from sows without reproductive failure in Argentina

Porcine parvovirus (PPV) is one of many pathogens responsible for reproductive failure in pregnant sows. Several studies have reported the appearance of new PPV strains that differ from previous isolates both genetically and antigenically. Thus, the protective effects of commercially inactivated vaccines could not be complete. In South America, the information about PPV is limited. Thus, the aim of the present study was to detect and characterize the PPV strains present in 131 mummies or stillbirths from normal deliveries in sows from a commercial swine farm of Argentina that uses the commercial vaccine. PCR results showed that 17/131 were positive to PPV. Ten of these viruses were isolated and sequenced. All viruses were related to the PPV1 sequence (NADL-2), maintaining the amino acid differences in positions 436 (S–P) and 565 (R–K). This study is the first to report the isolation of PPV in Argentina and the results suggest that PPV can cross the placenta even in vaccinated sows, thus affecting some of the fetuses and being able to cause fetal death in sows without reproductive failure. The results also suggest that vaccination only reduces clinical signs and reproductive disorders and may thus not be a perfect tool to manage PPV infection. This study provides information that needs to be studied in depth to improve strategies to prevent and control PPV infection in swine farms.Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Cordoba. Estacion Experimental Agropecuaria Marcos Juarez. Agencia de Extension Rural Rio Cuarto.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Dibárbora, Marina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Cordoba. Estacion Experimental Agropecuaria Marcos Juarez. Agencia de Extension Rural Rio Cuarto.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Calderón, M. Gallo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Echeverría, M. G.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin

Automatic Processing of place value in four digit numbers depends on number complexity

Coding of place-value of numbers is as relevant in number processing as coding the identity of the corresponding digits. Recently, Kallai & Tzelgov (2012), manipulating physical size and numerical magnitude in a physical size decision task, found longer response times when the numerical magnitude of four-digit numbers was not congruent with its physical size. They concluded that place-value I multi-digit numbers is processed automatically. However, these authors, with the aim of isolating the effect of place-value, used exclusively strings composed by three zeros and one non-zero digit that varied its position within the string (e.g., 0060 vs. 0600), hence it is uncertain whether automatic coding of place-value van be extended to more complex number strings (e.g., 1364 vs 1634). In the present research, together with Kallai & Tzelgov’s stimuli, numbers composed of four different non-zero digits (e.g., 7192 vs. 7912) were employed. Fifty-nine participants were requested to decide which of the two four-digit length numbers was presented in bigger font. Results using Kallai & Tzelgov’s stimuli replicated the findings of these authors. However, no evidence of place-value processing was found with the all-different stimuli. Automatic coding of place value seems to be affected by the complexity of numbers to be processed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Targeted knockdown of Cerkl, a retinal dystrophy gene, causes mild affectation of the retinal ganglion cell layer

AbstractIn order to approach the function of the retinal dystrophy CERKL gene we generated a novel knockout mouse model by cre-mediated targeted deletion of the Cerkl first exon and proximal promoter. The excised genomic region (2.3kb) encompassed the first Cerkl exon, upstream sequences including the proximal promoter and the initial segment of the first intron. The Cerkl−/− mice were viable and fertile. The targeted Cerkl deletion resulted in a knockdown more than a knockout model, given that alternative promoters (unreported at that time) directed basal expression of Cerkl (35%). In situ hybridizations and immunohistochemistry showed that this remnant expression was moderate in the photoreceptors and weak in the ganglion and inner cell layers. Morphological characterization of the Cerkl−/− retinas did not show any gross structural changes, even at 12months of age. However, some clear and consistent signals of gliosis and retinal stress were detected by the statistically significant increase of i) the glial fibrillary antigen protein (GFAP) expression, and ii) apoptosis, as detected by TUNEL. Remarkably, consistent non-progressive perturbation (from birth up to 12months of age) of ganglion cells was supported by the decrease of the Brn3a marker expression as well as the reduced oscillatory potentials in the electroretinographic recordings. In conclusion, the Cerkl−/− knockdown shows a mild retinal phenotype, with increased levels of cellular stress and apoptosis indicators, and clear signs of functional alteration at the ganglion cell layer, but no detectable morphological changes

Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonicpasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss inthe pig industry. This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate theantibiotic susceptibility profiles of the strains.Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine speciesspecific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibioticresistance profiles. We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene.The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin,tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprimsulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin(ERY), TMS, SPC, OTC, FFN, TIL, and CTF. The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the mostfrequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.Fil: Bessone, Fernando Anibal. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Soriano Pérez, María Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Dibárbora, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Characterization of new strains of Pseudorabies virus in Argentina: Detection of interspecies transmission

Background: Aujeszky's disease is mainly a swine disease, still endemic worldwide. It can infect other mammalians, including human beings, and it is usually fatal with nervous symptoms. Ever since the disease was detected in 1988 in Argentina, many outbreaks have been reported involving both feral swine and dogs. Aim: At present, in Argentina, Pseudorabies virus (PRV) cases are sporadically reported; however, clinical cases are informed. This study aims to obtain information about the seroprevalence of PRV in wild boars and to isolate and characterize PRV from clinical samples. Methods: From 2018 to 2019, 78 wild boars’ serum samples from Bahía de Samborombón natural reserve were analyzed for antibodies to PRV using a virus neutralization test. Clinical samples from 17 pigs, 2 wild boars, 1 dog, and 1 cat were collected from 2013 to 2019 for viral isolation and detection of the presence of the gD gene by PCR. For sequence analysis, the gC partial gene was amplified. Results: Five strains were isolated from the dog, cat, and swine samples. The new PRV strains identified were confirmed by BLAST analysis, which revealed between 99.74% and 100% of similarity to the NIA-3 strain and phylogenetic analysis of the partial gene encoding the gC protein revealed that the PRV strains have divided into two main clades, clade 1 and clade 2. Conclusion: This report informed that most new cases of PRV were detected in the central regions of Argentina, where pig production is concentrated. The study in Bahía de Samborombón revealed a high percentage of detection but, the sampling is not representative of that of the rest of the country. Therefore, a systematic sampling effort of wild boar throughout the country should be included in the national program control. Although in Argentina only the inactivated Bartha vaccine is allowed, recombination risk should not be ignored if attenuated vaccines are incorporated into the National control plan. The two strains, one from the cat and one from the dog sample, are directly related to infected swine. The information about clinical cases and molecular characterization of new strains is important for a better understanding of the dynamics of PRV and to promote preventive measures.Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Cappuccio, Javier Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Fossaroli, Melisa Gisele. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Williman, Macarena Marta. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Dibárbora, Marina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Brizzio, Renata. Universidad Católica de Córdoba; ArgentinaFil: Metz, German Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Aspitia, Carolina Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Pérez, Alejandro. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Carpinetti, Bruno Nicolás. Universidad Nacional Arturo Jauretche. Instituto Ciencias Sociales y Administracion; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentin

Design, characterization and in vitro evaluation of antifungal polymeric films

The objective of the present paper was the development and the full characterization of antifungal films. Econazole nitrate (ECN) was loaded in a polymeric matrix formed by chitosan (CH) and carbopol 971NF (CB). Polyethylene glycol 400 and sorbitol were used as plasticizing agents. The mechanical properties of films were poorer when the drug was loaded, probably because crystals of ENC produces network outages and therefore reduces the polymeric interactions between the polymers. Polymers–ECN and CH–CB interactions were analyzed by Fourier-transform infrared spectroscopy (FTIR), thermal gravimetry analysis, and differential thermal analysis (DTA-TGA). ECN did not show structure alterations when loaded into the films. In scanning electron microphotographs and atomic force microscopy analysis, films prepared with CB showed an evident wrinkle pattern probably due to the strong interactions between the polymers, which were observed by FTIR and DTA-TGA. The in vitro activity of the formulations against Candida krusei and Candida parapsilosis was twice as greater as the commercial cream, probably as a result of the antifungal combination of the drug with the CH activity. All these results suggest that these polymeric films containing ECN are potential candidates in view of alternatives dosages forms for the treatment of the yeast assayed.Fil: Real, Daniel Andres. Universidad Nacional de Rosario; ArgentinaFil: Martinez, Maria V.. Universidad Nacional de Rosario; ArgentinaFil: Frattini, Agustin Lujan. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Soazo, Marina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; ArgentinaFil: Luque, Alicia G.. Universidad Nacional de Rosario; ArgentinaFil: Biasoli, Marisa S.. Universidad Nacional de Rosario; ArgentinaFil: Salomon, Claudio Javier. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; ArgentinaFil: Olivieri, Alejandro Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; ArgentinaFil: Leonardi, Darío. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentin