Data Descriptor: High resolution, week-long, locomotion time series from Japanese quail in a home-box environment

Temporal and spatial patterns of locomotion reflect both resting periods and the movement from one place to another to satisfy physiological and behavioural needs. Locomotion is studied in diverse areas of biology such as chronobiology and physiology, as well as in biomathematics. Herein, the locomotion of 24 visually-isolated Japanese quails in their home-box environment was recorded continuously over a 6.5 days at a 0.5 s sampling rate. Three time series are presented for each bird: (1) locomotor activity, (2) distance ambulated, and (3) zone of the box where the bird is located. These high resolution, week-long, time series consisting of 1.07 × 10 6 data points represent, to our knowledge, a unique data set in animal behavior, and are publically available on FigShare. The data obtained can be used for analyzing dynamic changes of daily or several day locomotion patterns, or for comparison with existing or future data sets or mathematical models across different taxa.publishedVersionFil: Flesia, Ana Georgina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina.Fil: Flesia, Ana Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Centro de Investigaciones y Estudios de Matemática; Argentina.Fil: Guzmán, Diego A. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Química Biológica; Argentina.Fil: Guzmán, Diego A. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.Fil: Guzmán, Diego A. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Ciencia y Tecnología de los Alimentos; Argentina.Fil: Pellegrini, Stefania. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Química Biológica; Argentina.Fil: Pellegrini, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.Fil: Pellegrini, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Ciencia y Tecnología de los Alimentos; Argentina.Fil: Aon, Miguel A. Johns Hopkins University. School of Medicine; Estados Unidos de AméricaFil: Marin, Raúl H. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Química Biológica; Argentina.Fil: Marin, Raúl H. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.Fil: Marin, Raúl H. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Ciencia y Tecnología de los Alimentos; Argentina.Fil: Kembro, Jackelyn M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales, Cátedra de Química Biológica; Argentina.Fil: Kembro, Jackelyn M. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina.Fil: Kembro, Jackelyn M. Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Córdoba. Instituto de Ciencia y Tecnología de los Alimentos; Argentina.Fil: Guzmán, Diego A. Aarhus University. Department of Animal Science; Dinamarca

Evaluation of the risks of the professionally exposed staff after the expansion of the storage of corrosive raw materials

[EN] This work deals with the expansion of the storage of corrosive raw materials in a company dedicated to the manufacture and packaging of ecological fertilizers, carried out in accordance with the new Regulation of Storage of Chemical Products, in force since September 2017. To secure the safety and health of the workers of the company with access to the storage, an identification and evaluation of the main risks of the professionally exposed workers in the raw materials storage is done. After this, the preventive measures to be applied will be proposed, so that the work carried out in the raw materials warehouse is done under optimal safety conditions.[ES] Este trabajo aborda la ampliación del almacenamiento de materias primas corrosivas de una empresa dedicada a la fabricación y envasado de fertilizantes ecológicos, realizado de acuerdo con el nuevo Reglamento de Almacenamiento de Productos Químicos, en vigor desde septiembre de 2017. Para garantizar la seguridad y salud de los trabajadores de la empresa con acceso a dicho almacenamiento, se realizará una identificación y evaluación de los principales riesgos del personal profesionalmente expuesto del almacén de materias primas. Tras ello, se propondrán las medidas preventivas a aplicar, con la finalidad de que el trabajo realizado en el almacén de materias primas se realice bajo unas condiciones de seguridad óptimas.Ferrer Marin, J.; Arnal Arnal, JM.; García-Fayos, B.; Sancho, M. (2019). Evaluación de los riesgos del personal profesionalmente expuesto tras la ampliación de un almacén de materias primas corrosivas. AEIPRO. 1754-1766. http://hdl.handle.net/10251/181195S1754176

Andes Basin Focal Project

The CPWF Basin Focal Project for the Andes system of basins worked with a range of local stakeholders to develop a better understanding of the mechanisms for improving the productivity of water in the Andes. We considered productivity in broad terms as the productivity of energy (HEP), food and fiber (agriculture) and livelihoods (industry, transport and benefit sharing such as Payments for Environmental Services schemes (PES)). In addition to the compiled data bases and analyses on poverty and institutions, one of the key deliverables of the project was the development and deployment of the AguAAndes policy support system (PSS). This integrates analyses of water availability and productivity within the local environmental and policy context. It is a web-based policy support system combining an extensive spatial database with process-based models for hydrology, crop production and socio-economic processes. It is intended to allow analysts and decision makers to test the potential onsite and offsite impacts of land and water management decisions in terms of their ability to sustain environmental services and human wellbeing. Interventions and recommendations for future actions on water and food in the region are presented

Two-channel dansyl/tryptophan emitters with a cholic acid bridge as reporters for local hydrophobicity within supramolecular systems based on bile salts

The aim of the present work is to develop two-channel emitters to probe local hydrophobicity by means of fluorescence quenching within different biomimetic supramolecular environments. To achieve this goal, the dansyl (Dns) and tryptophan (Trp) fluorophores have been covalently attached to cholic acid (CA) in order to ensure simultaneous incorporation of the two emitting units into the same compartment. In principle, the two fluorophores of the synthesized Dns-CA-Trp probes could either exhibit an orthogonal behavior or display excited state interactions. The fluorescence spectra of 3 beta-Dns-CA-Trp showed a residual Trp emission band at ca. 350 nm and an enhanced Dns maximum in the 500-550 nm region. This reveals a partial intramolecular energy transfer, which is consistent with the Dns and Trp singlet energies. Thus, the two photoactive units are not orthogonal; nevertheless, 3 beta-Dns-CA-Trp seems appropriate as a two-channel reporter for the supramolecular systems of interest. Fluorescence quenching of 3 beta-Dns-CA-Trp by iodide (which remains essentially in bulk water) was examined within sodium cholate, sodium taurocholate, sodium deoxycholate and mixed micelles. Interestingly, a decrease in the emission intensity of the two bands was observed with increasing iodide concentrations. The most remarkable effect was observed for mixed micelles, where the quenching rate constants were one order of magnitude lower than in solution. As anticipated, the quenching efficiency by iodide decreased with increasing hydrophobicity of the microenvironment, a trend that can be correlated with the relative accessibility of the probe to the ionic quencher.Financial support from the Spanish Government (CTQ2012-38754-C03-03), Predoctoral FPU fellowship (AP2008-03295), and the Generalitat Valenciana (Prometeo Program) is gratefully acknowledged.Gómez Mendoza, M.; Marín García, ML.; Miranda Alonso, MÁ. (2014). Two-channel dansyl/tryptophan emitters with a cholic acid bridge as reporters for local hydrophobicity within supramolecular systems based on bile salts. Organic and Biomolecular Chemistry. 12(42):8499-8504. https://doi.org/10.1039/c4ob01394hS849985041242Vayá, I., Lhiaubet-Vallet, V., Jiménez, M. C., & Miranda, M. A. (2014). Photoactive assemblies of organic compounds and biomolecules: drug–protein supramolecular systems. Chem. Soc. Rev., 43(12), 4102-4122. doi:10.1039/c3cs60413fGomez-Mendoza, M., Marin, M. L., & Miranda, M. A. (2011). Dansyl Derivatives of Cholic Acid as Tools to Build Speciation Diagrams for Sodium Cholate Aggregation. The Journal of Physical Chemistry Letters, 2(7), 782-785. doi:10.1021/jz200178rGomez-Mendoza, M., Marin, M. L., & Miranda, M. A. (2012). Dansyl-Labeled Cholic Acid as a Tool To Build Speciation Diagrams for the Aggregation of Bile Acids. The Journal of Physical Chemistry B, 116(51), 14776-14780. doi:10.1021/jp308624hWaissbluth, O. L., Morales, M. C., & Bohne, C. (2006). Influence of Planarity and Size on Guest Binding with Sodium Cholate Aggregates. Photochemistry and Photobiology, 82(4), 1030. doi:10.1562/2006-02-14-ra-803Rinco, O., Kleinman, M. H., & Bohne, C. (2001). Reactivity of Benzophones in the Different Binding Sites of Sodium Cholate Aggregates. Langmuir, 17(19), 5781-5790. doi:10.1021/la010526cHofmann, A. F. (1999). The Continuing Importance of Bile Acids in Liver and Intestinal Disease. Archives of Internal Medicine, 159(22), 2647. doi:10.1001/archinte.159.22.2647Nuin, E., Gómez-Mendoza, M., Andreu, I., Marin, M. L., & Miranda, M. A. (2012). New Photoactive Compounds To Probe Cholic Acid and Cholesterol inside Mixed Micelles. Organic Letters, 15(2), 298-301. doi:10.1021/ol303201yHammad, M. ., & Müller, B. . (1998). Increasing drug solubility by means of bile salt–phosphatidylcholine-based mixed micelles. European Journal of Pharmaceutics and Biopharmaceutics, 46(3), 361-367. doi:10.1016/s0939-6411(98)00037-xHammad, M. ., & Müller, B. . (1998). Solubility and stability of tetrazepam in mixed micelles. European Journal of Pharmaceutical Sciences, 7(1), 49-55. doi:10.1016/s0928-0987(98)00006-2Hammad, M. (1998). Solubility and stability of clonazepam in mixed micelles. International Journal of Pharmaceutics, 169(1), 55-64. doi:10.1016/s0378-5173(98)00117-3Hendradi, E., Obata, Y., Isowa, K., Nagai, T., & Takayama, K. (2003). Effect of Mixed Micelle Formulations Including Terpenes on the Transdermal Delivery of Diclofenac. Biological & Pharmaceutical Bulletin, 26(12), 1739-1743. doi:10.1248/bpb.26.1739Parsaee, S., Sarbolouki, M. N., & Parnianpour, M. (2002). In-vitro release of diclofenac diethylammonium from lipid-based formulations. International Journal of Pharmaceutics, 241(1), 185-190. doi:10.1016/s0378-5173(02)00238-7Yu, J., Zhu, Y., Wang, L., Peng, M., Tong, S., Cao, X., … Xu, X. (2010). Enhancement of oral bioavailability of the poorly water-soluble drug silybin by sodium cholate/phospholipid-mixed micelles. Acta Pharmacologica Sinica, 31(6), 759-764. doi:10.1038/aps.2010.55Sznitowska, M., Klunder, M., & Placzek, M. (2008). Paclitaxel Solubility in Aqueous Dispersions and Mixed Micellar Solutions of Lecithin. CHEMICAL & PHARMACEUTICAL BULLETIN, 56(1), 70-74. doi:10.1248/cpb.56.70Nuin, E., Gomez-Mendoza, M., Marin, M. L., Andreu, I., & Miranda, M. A. (2013). Influence of Drug Encapsulation within Mixed Micelles on the Excited State Dynamics and Accessibility to Ionic Quenchers. The Journal of Physical Chemistry B, 117(32), 9327-9332. doi:10.1021/jp404353uCuquerella, M. C., Rohacova, J., Marin, M. L., & Miranda, M. A. (2010). Stereodifferentiation in fluorescence quenching within cholic acid aggregates. Chemical Communications, 46(27), 4965. doi:10.1039/c0cc00176gWu, J., Liu, W., Ge, J., Zhang, H., & Wang, P. (2011). New sensing mechanisms for design of fluorescent chemosensors emerging in recent years. Chemical Society Reviews, 40(7), 3483. doi:10.1039/c0cs00224kBronshtein, I., Afri, M., Weitman, H., Frimer, A. A., Smith, K. M., & Ehrenberg, B. (2004). Porphyrin Depth in Lipid Bilayers as Determined by Iodide and Parallax Fluorescence Quenching Methods and Its Effect on Photosensitizing Efficiency. Biophysical Journal, 87(2), 1155-1164. doi:10.1529/biophysj.104.041434Rohacova, J., Marin, M. L., Martínez-Romero, A., O’Connor, J.-E., Gomez-Lechon, M. J., Donato, M. T., … Miranda, M. A. (2009). Synthesis of new, UV-photoactive dansyl derivatives for flow cytometric studies on bile acid uptake. Organic & Biomolecular Chemistry, 7(23), 4973. doi:10.1039/b912134jFőrster, T. (1959). 10th Spiers Memorial Lecture. Transfer mechanisms of electronic excitation. Discuss. Faraday Soc., 27(0), 7-17. doi:10.1039/df9592700007Eaton, D. F. (1988). Reference materials for fluorescence measurement. Pure and Applied Chemistry, 60(7), 1107-1114. doi:10.1351/pac198860071107T. Förster , Modern Quantum Chemistry , Academic Press , New York , 196

EL PASO DEL CONCEPTO DE CONVIVENCIA CON LA SEMI-ARIDEZ A LA APLICACIÓN DE POLÍTICAS-PAÍS: BRASIL 2002-2016

El presente artículo objetivó explorar si ha sido posible hacer realidad la propuesta brasileña de convivencia con la semi-aridez, y de haber sido así, establecer cuáles estrategias han contribui- do, y cuáles condiciones gruesas han sido necesarias para ello. En contexto, fue realizado un análi- sis comparativo, de las transformaciones que ocurrieron en 10 territorios del semiárido brasileño, en dos periodos de tiempo PI (1973-2001) con políticas de combate a las sequias vs PII (2002- 2016) con la implementación de políticas gubernamentales de desarrollo bajo el concepto de convivencia con la semi-áridez. Las variables y procesos analizados incluyeron (a) Capital Físico; (b) Acceso a Infra-Estructura Hídrica; (c) Diversificación de sistemas de Producción (vegetal y ani- mal); (d) Gestión de Bienes Común; (e) Integración en Espacios Políticos Organizativos y (f ) Acceso a Políticas Públicas. Los resultados obtenidos muestran que hubo cambios significativos en los in- dicadores analizados en el periodo PII, en comparación al periodo de (PI), en los 10 territorios ana- lizados, como resultado del acceso a políticas públicas de convivencia con la sequía. Comparando el P-II con el P-I, en promedio, hubo una mejoría sustantiva en el Acceso a Infra-estructura Hídrica (121%), Diversidad de los sistemas Producción (Animal, 99% y Vegetal 229%), Participación en la Gestión de Bienes Comunes (66%), Integración en Espacio Políticos Organizativos (148%) y Participación en Políticas Publicas (99%). La convivencia pasó de ser un concepto a convertirse en hecho como consecuencia del conjunto de transformaciones estructurales, agroecológicas, sociales y de manejo en combinación con el fortalecimiento de mecanismos de reciprocidad co- munitaria, caracterizados por a) movilización social y canalización para crear almacenamiento de recursos con el fin de utilizarlos en periodos de sequias, b) la reducción de la pérdida de recursos de los agro-ecosistemas y uso su eficaz y c) la articulación, organización y momentos sinérgicos de comunicación entre los diversos atores

Heterogeneity of Melanoma Cell Responses to Sleep Apnea-Derived Plasma Exosomes and to Intermittent Hypoxia

Obstructive sleep apnea (OSA) is associated with increased cutaneous melanoma incidence and adverse outcomes. Exosomes are secreted by most cells, and play a role in OSA-associated tumor progression and metastasis. We aimed to study the effects of plasma exosomes from OSA patients before and after adherent treatment with continuous positive airway pressure (CPAP) on melanoma cells lines, and also to identify exosomal miRNAs from melanoma cells exposed to intermittent hypoxia (IH) or normoxia. Plasma-derived exosomes were isolated from moderate-tosevere OSA patients before (V1) and after (V2) adherent CPAP treatment for one year. Exosomes were co-incubated with three3 different melanoma cell lines (CRL 1424; CRL 1619; CRL 1675) that are characterized by genotypes involving different mutations in BRAF, STK11, CDKN2A, and PTEN genes to assess the effect of exosomes on cell proliferation and migration, as well as on pAMK activity in the presence or absence of a chemical activator. Subsequently, CRL-1424 and CRL-1675 cells were exposed to intermittent hypoxia (IH) and normoxia, and exosomal miRNAs were identified followed by GO and KEG pathways and gene networks. The exosomes from these IH-exposed melanoma cells were also administered to THP1 macrophages to examine changes in M1 and M2 polarity markers. Plasma exosomes from V1 increased CRL-1424 melanoma cell proliferation and migration compared to V2, but not the other two cell lines. Exposure to CRL-1424 exosomes reduced pAMPK/tAMPK in V1 compared to V2, and treatment with AMPK activator reversed the effects. Unique exosomal miRNAs profiles were identified for CRL-1424 and CRL-1675 in IH compared to normoxia, with six miRNAs being regulated and several KEGG pathways were identified. Two M1 markers (CXCL10 and IL6) were significantly increased in monocytes when treated with exosomes from IH-exposed CRL-1424 and CRL-1625 cells. Our findings suggest that exosomes from untreated OSA patients increase CRL-1424 melanoma malignant properties, an effect that is not observed in two other melanoma cell lines. Exosomal cargo from CRL-1424 cells showed a unique miRNA signature compared to CRL-1675 cells after IH exposures, suggesting that melanoma cells are differentially susceptible to IH, even if they retain similar effects on immune cell polarity. It is postulated that mutations in STK-11 gene encoding for the serine/threonine kinase family that acts as a tumor suppressor may underlie susceptibility to IH-induced metabolic dysfunction, as illustrated by CRL1424 cells

A novel synthetic approach to tyrosine dimers based on pterin photosensitization

Oxidative damage to proteins leads to a variety of modifications that are markers of pathogenesis. One of the most important modifications is the dityrosine (Tyr₂ ) cross-link, resulting from an oxidative covalent bond between two tyrosines (Tyr). An optimized methodology for preparation of pure Tyr₂ is important to investigate in detail its physicochemical properties and reactivity. Pterin (Ptr), the parent and unsubstituted compound of oxidized pterins, is able to photosensitize the cross-linking of free tyrosine (Tyr) and tyrosine residues of peptides and proteins through a photoinduced electron transfer mechanism. We have optimized a simple, one-step photocatalyzed formation of Tyr₂ , using Ptr as photocatalyst. Our procedure is carried out in aqueous solutions under UV-A radiation for few minutes. The purification of Tyr₂ is performed by reverse-phase chromatography. The obtained highly pure solution is used to fully characterize the Tyr₂ (exact mass and ¹H, ¹H- ¹H COSY; DEPT; HSQC and HMBC NMR experiments) and to deeper study its fluorescence properties.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicada