The rest-frame optical sizes of massive galaxies with suppressed star formation at z∼4z\sim4

We present the rest-frame optical sizes of massive quiescent galaxies (QGs) at $z\sim4$ measured at $K'$-band with the Infrared Camera and Spectrograph (IRCS) and AO188 on the Subaru telescope. Based on a deep multi-wavelength catalog in the Subaru XMM-Newton Deep Survey Field (SXDS), covering a wide wavelength range from the $u$-band to the IRAC $8.0\mu m$ over 0.7 deg$^2$, we evaluate photometric redshift to identify massive ($M_{\star}\sim10^{11}\ M_\odot$) galaxies with suppressed star formation. These galaxies show a prominent 4000$\rm \AA$break feature at$z\sim4$, suggestive of an evolved stellar population. We then conduct follow-up$K'$-band imaging with adaptive optics for the five brightest galaxies ($K_{AB,total}=22.5\sim23.4$). Compared to lower redshift ones, QGs at$z\sim4$have smaller physical sizes of effective radii$r_{eff}=0.2$to$1.8$kpc. The mean size measured by stacking the four brightest objects is$r_{eff}=0.7\rm\ kpc$. This is the first measurement of the rest-frame optical sizes of QGs at$z\sim4$. We evaluate the robustness of our size measurements using simulations and find that our size estimates are reasonably accurate with an expected systematic bias of$\sim0.2$kpc. If we account for the stellar mass evolution, massive QGs at$z\sim4$are likely to evolve into the most massive galaxies today. We find their size evolution with cosmic time in a form of$\log(r_e/{\rm kpc})= -0.44+1.77 \log(t/\rm Gyr)$. Their size growth is proportional to the square of stellar mass, indicating the size-stellar mass growth driven by minor dry mergers.Comment: 15 pages, 11 figures, ApJ accepte

Potential of Genomic Selection in Mass Selection Breeding of an Allogamous Crop: An Empirical Study to Increase Yield of Common Buckwheat

To evaluate the potential of genomic selection (GS), a selection experiment with GS and phenotypic selection (PS) was performed in an allogamous crop, common buckwheat (Fagopyrum esculentum Moench). To indirectly select for seed yield per unit area, which cannot be measured on a single-plant basis, a selection index was constructed from seven agro-morphological traits measurable on a single plant basis. Over 3 years, we performed two GS and one PS cycles per year for improvement in the selection index. In GS, a prediction model was updated every year on the basis of genotypes of 14,598–50,000 markers and phenotypes. Plants grown from seeds derived from a series of generations of GS and PS populations were evaluated for the traits in the selection index and other yield-related traits. GS resulted in a 20.9% increase and PS in a 15.0% increase in the selection index in comparison with the initial population. Although the level of linkage disequilibrium in the breeding population was low, the target trait was improved with GS. Traits with higher weights in the selection index were improved more than those with lower weights, especially when prediction accuracy was high. No trait changed in an unintended direction in either GS or PS. The accuracy of genomic prediction models built in the first cycle decreased in the later cycles because the genetic bottleneck through the selection cycles changed linkage disequilibrium patterns in the breeding population. The present study emphasizes the importance of updating models in GS and demonstrates the potential of GS in mass selection of allogamous crop species, and provided a pilot example of successful application of GS to plant breeding

A massive quiescent galaxy in a group environment at z=4.53z=4.53

We report on the spectroscopic confirmation of a massive quiescent galaxy at $z_\mathrm{spec}=4.53$ in the COSMOS field with Keck/MOSFIRE. The object was first identified as a galaxy with suppressed star formation at $z_\mathrm{phot}\sim4.65$ from the COSMOS2020 catalog. The follow-up spectroscopy with MOSFIRE in the $K$-band reveals a faint [OII] emission and the Balmer break, indicative of evolved stellar populations. We perform the spectral energy distribution fitting using both the photometry and spectrum to infer physical properties. The obtained stellar mass is high ($M_*\sim 10^{10.8}\,M_\odot$) and the current star formation rate is more than 1 dex below that of main-sequence galaxies at $z=4.5$. Its star formation history suggests that this galaxy experienced starburst at $z\sim5$ followed by a rapid quenching phase. This is one of the youngest quiescent galaxies at $z>3$ and is likely a galaxy in the process of being quenched. An unique aspect of the galaxy is that it is in an extremely dense region; there are four massive star-forming galaxies at $4.4<z_\mathrm{phot}<4.7$ located within 150 physical kpc from the galaxy. Interestingly, three of them have strongly overlapping virial radii with that of the central quiescent galaxy ($\sim 70\,\mathrm{kpc}$), suggesting that the over-density region is likely the highest redshift candidate of a dense group with a spectroscopically confirmed quiescent galaxy at the center. The group provides us with an unique opportunity to gain insights into the role of the group environment for quenching at $z\sim$ 4 - 5 corresponding to the formation epoch of massive elliptical galaxies in the local Universe.Comment: 13 pages, 7 figures, 2 tables; submitted to Ap

Stellar Velocity Dispersion of a Massive Quenching Galaxy at z = 4.01

We present the first stellar velocity dispersion measurement of a massive quenching galaxy at z = 4. The galaxy is first identified as a massive z ≥ 4 galaxy with suppressed star formation from photometric redshifts based on deep multiband data. A follow-up spectroscopic observation with MOSFIRE on Keck revealed strong multiple absorption features, which are identified as Balmer lines, giving a secure redshift of z = 4.01. This is the most distant quiescent galaxy known to date. Thanks to the high S/N of the spectrum, we are able to estimate the stellar velocity dispersion, σ=268±59 km s⁻¹, making a significant leap from the previous highest redshift measurement at z = 2.8. Interestingly, we find that the velocity dispersion is consistent with that of massive galaxies today, implying no significant evolution in velocity dispersion over the last 12 Gyr. Based on a stringent upper limit on its physical size from deep optical images (r_(eff) < 1.3 kpc), we find that its dynamical mass is consistent with the stellar mass inferred from photometry. Furthermore, the galaxy is located on the mass fundamental plane extrapolated from lower redshift galaxies. The observed no strong evolution in σ suggests that the mass in the core of massive galaxies does not evolve significantly, while most of the mass growth occurs in the outskirts of the galaxies, which also increases the size. This picture is consistent with a two-phase formation scenario in which mass and size growth is due to accretion in the outskirts of galaxies via mergers. Our results imply that the first phase may be completed as early as z ~ 4

Rapid genotyping with DNA micro-arrays for high-density linkage mapping and QTL mapping in common buckwheat (Fagopyrum esculentum Moench)

For genetic studies and genomics-assisted breeding, particularly of minor crops, a genotyping system that does not require a priori genomic information is preferable. Here, we demonstrated the potential of a novel array-based genotyping system for the rapid construction of high-density linkage map and quantitative trait loci (QTL) mapping. By using the system, we successfully constructed an accurate, high-density linkage map for common buckwheat (Fagopyrum esculentum Moench); the map was composed of 756 loci and included 8,884 markers. The number of linkage groups converged to eight, which is the basic number of chromosomes in common buckwheat. The sizes of the linkage groups of the P1 and P2 maps were 773.8 and 800.4 cM, respectively. The average interval between adjacent loci was 2.13 cM. The linkage map constructed here will be useful for the analysis of other common buckwheat populations. We also performed QTL mapping for main stem length and detected four QTL. It took 37 days to process 178 samples from DNA extraction to genotyping, indicating the system enables genotyping of genome-wide markers for a few hundred buckwheat plants before the plants mature. The novel system will be useful for genomics-assisted breeding in minor crops without a priori genomic information

Co-activation of macrophages and T cells contribute to chronic GVHD in human IL-6 transgenic humanised mouse model.

BACKGROUND: Graft-versus host disease (GVHD) is a complication of stem cell transplantation associated with significant morbidity and mortality. Non-specific immune-suppression, the mainstay of treatment, may result in immune-surveillance dysfunction and disease recurrence. METHODS: We created humanised mice model for chronic GVHD (cGVHD) by injecting cord blood (CB)-derived human CD34 FINDINGS: In cGVHD humanised mice, we found activation of T cells in the spleen, lung, liver, and skin, activation of macrophages in lung and liver, and loss of appendages in skin, obstruction of bronchioles in lung and portal fibrosis in liver recapitulating cGVHD. Acute GVHD humanised mice showed activation of T cells with skewed TCR repertoire without significant macrophage activation. INTERPRETATION: Using humanised mouse models, we demonstrated distinct immune mechanisms contributing acute and chronic GVHD. In cGVHD model, co-activation of human HSPC-derived macrophages and T cells educated in the recipient thymus contributed to delayed onset, multi-organ disease. In acute GVHD model, mature human T cells contained in the graft resulted in rapid disease progression. These humanised mouse models may facilitate future development of new molecular medicine targeting GVHD

Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice.

The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors that foster functional maturation of immune cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation of human immunity in vivo, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune function. To study the roles of human cytokines in human acquired and innate immune cell development, we created NSG mice expressing hIL-7 and hIL-15. Although hIL-7 alone was not sufficient for supporting human NK cell development in vivo, increased frequencies of human NK cells were confirmed in multiple organs of hIL-7 and hIL-15 double knockin (hIL-7xhIL-15 KI) NSG mice engrafted with human hematopoietic stem cells. hIL-7xhIL-15 KI NSG humanized mice provide a valuable in vivo model to investigate development and function of human NK cells

Stellar Velocity Dispersion of a Massive Quenching Galaxy at z = 4.01

We present the first stellar velocity dispersion measurement of a massive quenching galaxy at z = 4. The galaxy is first identified as a massive z ≥ 4 galaxy with suppressed star formation from photometric redshifts based on deep multiband data. A follow-up spectroscopic observation with MOSFIRE on Keck revealed strong multiple absorption features, which are identified as Balmer lines, giving a secure redshift of z = 4.01. This is the most distant quiescent galaxy known to date. Thanks to the high S/N of the spectrum, we are able to estimate the stellar velocity dispersion, σ=268±59 km s⁻¹, making a significant leap from the previous highest redshift measurement at z = 2.8. Interestingly, we find that the velocity dispersion is consistent with that of massive galaxies today, implying no significant evolution in velocity dispersion over the last 12 Gyr. Based on a stringent upper limit on its physical size from deep optical images (r_(eff) < 1.3 kpc), we find that its dynamical mass is consistent with the stellar mass inferred from photometry. Furthermore, the galaxy is located on the mass fundamental plane extrapolated from lower redshift galaxies. The observed no strong evolution in σ suggests that the mass in the core of massive galaxies does not evolve significantly, while most of the mass growth occurs in the outskirts of the galaxies, which also increases the size. This picture is consistent with a two-phase formation scenario in which mass and size growth is due to accretion in the outskirts of galaxies via mergers. Our results imply that the first phase may be completed as early as z ~ 4