26 research outputs found
Embedding a Metal-Binding Motif for Copper Transporter into a Lipid Bilayer by Cu(I) Binding
Peptide–lipid
interactions are widely involved with biologically
significant phenomena, including the pathogenic mechanisms of protein
misfolding diseases and transmembrane protein folding. In this paper,
the interaction of the cysteine/tryptophan (Cys/Trp) motif, which
is a metal-binding motif of copper transporter (Ctr) proteins, with
a lipid bilayer was studied using fluorescence and circular dichroism
(CD) spectroscopy. The binding of Cu(I) to the Cys/Trp motif induced
a large red-edge excitation shift in the Trp fluorescence, indicating
that the Trp residue is located inside the lipid bilayer following
complexation of Cu(I) with the Cys/Trp motif. The Stern–Volmer
quenching of the Trp fluorescence also supported the Cu(I) binding
peptide embedding in the lipid bilayer. The measurement of the CD
spectra indicated the increase in β-sheet content of the Cys/Trp
motif peptide as a result of Cu(I) binding. These results lead to
the conclusion that complexation with Cu(I) induces the change in
the secondary structure of the Cys/Trp motif, which results in the
peptide embedding in the lipid bilayer. Cu(I)-induced enhancement
of the lipid affinity is discussed in terms of the mechanism for copper
transport by Ctr
Collision detection on transmission lines with optical interferometer
V diplomski nalogi skušamo ugotoviti, v kolikšni meri je možno zaznavati in klasificirati trke na jeklenicah daljnovodov z optičnim interferometrom. Na začetku predstavimo osnovne pojme interferometrije in opišemo uporabljen optični interferometer. V jedru diplomske naloge natančneje opišemo eksperimentalni protokol in obdelavo signalov. Nadaljujemo z implementacijo algoritmov za segmentacijo in klasifikacijo zajetih signalov ter predstavimo dobljene rezultate. Segmentacijo izvedemo v domeni števila prehodov signala skozi ničlo, za klasifikacijo pa uporabimo večplastno nevronsko mrežo z algoritmom vzvratnega učenja. Rezultati študije nakazujejo, da sta implementirani segmentacija in klasifikacija uspešni v 77 % izvedenih trkov različnih predmetov.We analyse feasibility of collision detection on transmission lines with optical interferometer. We first provide a brief introduction into interferometry, along with a description of the optical interferometer used for measurements in this study. Afterwards, we describe the conducted experimental protocol and signal processing methodology. The focus is on implementation of algorithms for signal segmentation and collision classification. We used zero-crossing algorithm to transform signals into segmentation domain. Classification of collisions is done with a multilayer neural network trained by the backpropagation algorithm. The results demonstrate an average success rate of 77% for segmentation and classification of collision with five different objects
Sistematización de la experiencia de un ambiente de aprendizaje enriquecido por TIC durante la práctica clínica en fisioterapia cardiopulmonar en un hospital de nivel II de la ciudad de Cali
Esta investigación se centra en la caracterización de la experiencia de 4 estudiantes de fisioterapia de IX semestre de la Institución Universitaria Escuela Nacional del Deporte (IUEND) durante la implementación de un ambiente de aprendizaje enriquecido con Tecnologías de la Información y la Comunicación (TIC) en la práctica clínico – asistencial en Salud Cardiopulmonar; la cual se fundamenta en el hacer y pone a prueba las bases conceptuales del ciclo de fundamentación; todo esto con el fin de identificar las experiencias significativas que facilitan el aprendizaje y desarrollo de competencias clínicas, además analizar si este tipo de estrategias de enseñanza -aprendizaje permite al estudiante y al docente asesor superar inconvenientes propios de la práctica clínica como: optimizar tiempos de atención a pacientes, estudio independiente y trabajo colaborativo, retomar e integrar gran cantidad de conceptos y procedimientos aprendidos en IV semestre con las nuevas experiencias y la realidad del paciente; y a la vez cumplir con funciones administrativas propios del rol del fisioterapeuta asistencial (estadística, indicadores, desarrollo de guías, etc.) que dificultan el proceso de aprendizaje; concluyendo que los ambientes mediados por TIC pueden lograr superar estas dificultades y favorecer finalmente el aprendizaje significativo (juicio clínico), en el que se fundamenta el ciclo de práctica profesional
DRA001027
List of DRA (http://trace.ddbj.nig.ac.jp/dra/index_e.html) accession numbers
of the FANTOM5 samples, sequences and genomic coordinations.
Files are in tab-delimited format, which includes
* Library ID
* FFID
* BioSamples accession number
* DRA experiment accession number
* DRA run accession numbers
* DRA analysis accession number for genomic coordination (BAM file)
* DRA analysis accession number for CTSS (BED file)
* Experiment method (CAGE/RNA-Seq/sRNA-Seq
assay information of the mouse tissue samples for HeliScopeCAGE
a tab delimited flat file (SDRF file) describing the experimental details for mouse tissue samples for the standard protocol of the HeliScopeCAGE protoco
assay information of the mouse qualitycontrol samples for HeliScopeCAGE
a tab delimited flat file (SDRF file) describing the experimental details for mouse quality control samples for the standard protocol of the HeliScopeCAGE protoco
DRA002216
List of DRA (http://trace.ddbj.nig.ac.jp/dra/index_e.html) accession numbers
of the FANTOM5 samples, sequences and genomic coordinations.
Files are in tab-delimited format, which includes
* Library ID
* FFID
* BioSamples accession number
* DRA experiment accession number
* DRA run accession numbers
* DRA analysis accession number for genomic coordination (BAM file)
* DRA analysis accession number for CTSS (BED file)
* Experiment method (CAGE/RNA-Seq/sRNA-Seq
assay information of the unclassified samples for HeliScopeCAGE
a tab delimited flat file (SDRF file) describing the experimental details for unclassified samples for the standard protocol of the HeliScopeCAGE protoco
DRA001028
List of DRA (http://trace.ddbj.nig.ac.jp/dra/index_e.html) accession numbers
of the FANTOM5 samples, sequences and genomic coordinations.
Files are in tab-delimited format, which includes
* Library ID
* FFID
* BioSamples accession number
* DRA experiment accession number
* DRA run accession numbers
* DRA analysis accession number for genomic coordination (BAM file)
* DRA analysis accession number for CTSS (BED file)
* Experiment method (CAGE/RNA-Seq/sRNA-Seq
annotations of the mouse CAGE peaks
CAGE peak based table with annotated peaks for mouse sample