Chemical composition and thermal behaviour of tropical fat fractions from solvent-assisted process: a review

Fractional crystallization is a process applied to plant and animal fats to obtain fat products with new functional properties and nutritional values. Fractionation is generally performed through either a dry or a solvent-assisted process. As solvents can wash off the liquid molecules able to entrap the solid component inside, the solvent-crystallization seems to be more efficient than other fractionation procedures. In recent times, fractional crystallization has been investigated for avocado (Persea Americana) butter, engkabang (Shorea macrophylla) fat, palm oil-moringa oil blend, and mee fat (Madhuca longifolia) to prepare solid (stearin) and liquid (olein) fractions. This review sums up the main and recent published studies on chemical and thermal (upon differential scanning calorimetry) properties of fat fractions obtained using solvent-assisted process. It has been argued that the ease of a fat fractionation mainly depends on the thermal characteristics of its triacylglycerol molecules. Fats with low-and high-melting thermal transitions in wide separation would yield fat components with remarkable changes in physico-chemical characteristics, fatty acid and triacylglycerol compositions as well as thermal profiles. The activities involving the use of fat components, during food formulation, may significantly benefit from a further learning of the fats behavior

Differentiation of fractionated components of lard from other animal fats using different analytical techniques

A study was conducted to differentiate fractionated components of lard namely lard olein (LO) and lard stearin (LS) from other common animal fats. Lard fractions and animal fats were analyzed using differential scanning calorimetry (DSC), elemental analyzer–isotope ratio mass spectrometry (EA-IRMS), gas chromatography mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR). Overlay of FTIR spectra did not help to pinpoint any characteristic feature to distinguish either LO or LS from other animal fats, but overlay of DSC cooling curves helped a successful discrimination. The determination of δ13C from EA-IRMS showed that the values corresponding to the fractionated components of lard were significantly (p<0.05) different from those of the other common animal fats. GC-MS analysis showed that direct comparison of overall fatty acid data was not able to discriminate LO and LS from other animal fats, but the application of principal component analysis (PCA) to fatty acid data helped a successful discrimination

Diferenciación de acilgliceroles parciales derivados de diferentes grasas animales mediante técnicas de EA-IRMS y GCMS

A study was carried out to compare partial acylglycerols of lard with those of chicken fat, beef fat and mutton fat using Gas Chromatography Mass Spectrometry (GC-MS) and Elemental Analysis–Isotope Ratio Mass Spectrometry (EA-IRMS). Mono- (MAG) and di-(DAG) acylglycerols of animal fats were prepared according to a chemical glycerolysis method and isolated using column chromatography. The fatty acid composition and δ13C carbon isotope ratio of MAG and DAG derived from individual animal fat were determined separately to establish their identity characteristics. The results showed that the δ13C values of MAG and DAG of lard were significantly different from those of MAG and DAG derived from chicken fat, beef fat and mutton fat. According to the loading plots based on a principle component analysis (PCA), fatty acids namely stearic, oleic and linoleic were the most discriminating parameters to distinctly identify MAG and DAG derived from different animal fats. This demonstrated that the EA-IRMS and the PCA of fatty acid data have considerable potential for discriminating MAG and DAG derived from lard from other animal fats for Halal authentication purposes.Se realizó un estudio para comparar acilgliceroles parciales de la manteca de cerdo con las de grasa de pollo, grasa de vacuno y grasa de cordero utilizando cromatografía de gases-espectrometría de masas (GC-MS) y análisis elemental de Isótopos-Espectrometría de Masas (EA-IRMS). Los mono- (MAG) y di- (DAG) acilgliceroles de grasas animales se prepararon mediante un método de glicerolisis química y se aislaron mediante cromatografía en columna. La composición de ácidos grasos y la relación isotópica de carbono δ13C de los MAG y DAG de las grasas de animales se determinan por separado para establecer sus características de identidad. Los resultados mostraron que los valores de δ13C de MAG y DAG de la manteca de cerdo fue significativamente diferente de los de MAG y DAG derivados de grasa de pollo, grasa de vacuno y grasa de cordero. De acuerdo con los diagramas de carga basados en el análisis de componentes principales (PCA), los ácidos grasos esteárico, oleico y linoleico fueron los parámetros más exigentes para identificar claramente MAG y DAG derivados de las diferentes grasas animales. Esto demuestra que EA-IRMS y PCA de los datos de ácidos grasos tienen un potencial considerable en discriminar MAG y DAG derivados de la manteca de cerdo frente a otras grasas animales para fines de autenticación Halal

Use of gas liquid chromatography in combination with pancreatic lipolysis and multivariate data analysis techniques for identification of lard contamination in some vegetable oils

A study was conducted to investigate the use of gas liquid chromatography (GLC) to identify lard (LD) contamination in palm oil (PO), palm kernel oil (PKO), and canola oil (CLO). Vegetable oils were deliberately adulterated with animal fats such as LD, beef tallow (BT), and chicken fat (CF) in varying proportions. In order to monitor the fatty acid (FA) compositional changes due to adulteration, GLC analyses of fatty acid methyl esters (FAME) were performed on 2-monoacylglycerol (2-MG) and neutral triacylglycerol (TAG) isolated from each sample. For the evaluation of FA data, multivariate statistical techniques were employed. The results showed that canonical discriminant (CANDISC) analysis was the most effective technique for discriminating LD-adulterated samples from those adulterated with other animal fats. Additionally, mathematical equations obtained by simple regression analysis could be used for quantification of LD contents in admixtures

Physicochemical properties and thermal behavior of binary blends of Madhuca longifolia seed fat and palm oil as a lard substitute

Fat extracted from pork is prohibited under halal and kosher food regulations. A study was carried out on Madhuca longifolia seed fat and palm oil to compare their physicochemical, solidification and melting characteristics to formulate halal alternative lipid substitutes. Various blends of Madhuca longifolia (ML) and palm oil (PO) was formulated in order to become similar to lard (LD). A total of three binary blends were prepared: ML:PO (97:3; w/w), ML:PO (95:5), ML:PO (93:7), and identified by the mass ratio of ML to PO. The fat blends were compared with LD in terms of the fatty acid and triacylglycerol compositions using gas chromatography and high performance liquid chromatography, respectively. In addition, the fat blends also being studied for thermal properties using differential scanning calorimetry and solid fat content using p-nuclear magnetic resonance. Although there were considerable differences between LD and the fat blends with regard to fatty acid and triacylglycerol compositions, some similarities were seen regarding to thermal properties and solid fat content profiles. The blend of ML:PO (97:3) displayed closer similarity to LD with respect to melting transition at -3.59°C and its solid fat content profile showed the least difference to that of LD throughout the temperature range measured

Total phenolic content, antioxidative and antidiabetic properties of coconut (Cocos Nucifera L.) testa and selected bean seed coats

Natural alternatives tor the treatment of diabetes mellitus have been the interest of many researchers. In this study, the brown testas of mature coconuts were compared to beans seed coats of four varieties in terms of antioxidative and anti-hyperglycaemic properties. The total phenolic and flavonoid contents, the antioxidant potentials and the a-amvlase and a-glucosidase inhibitorv activities of the crude extracts were studied in vitro. The results showed that extracts of coconut testa and red kidney bean seed coat displayed higher a-glucosidase inhibition (IC50=19.90±5.67 and 4.84±1.43 μg/mL) and α-amylase inhibition (IC50=120.5±15.4 and 532.8±68.0 μg/mL) than the other extracts. These two extracts showed higher antioxidant capacities owing to their high phenolic and flavonoid contents. These results suggest that red kidney bean seed coat and tender coconut testa would have higher potential as nutraceuticals and could serve as natural alternative sources of anti-diabetic remedy