Exploring atmospheric radon with airborne gamma-ray spectroscopy

$^{222}$Rn is a noble radioactive gas produced along the $^{238}$U decay chain, which is present in the majority of soils and rocks. As $^{222}$Rn is the most relevant source of natural background radiation, understanding its distribution in the environment is of great concern for investigating the health impacts of low-level radioactivity and for supporting regulation of human exposure to ionizing radiation in modern society. At the same time, $^{222}$Rn is a widespread atmospheric tracer whose spatial distribution is generally used as a proxy for climate and pollution studies. Airborne gamma-ray spectroscopy (AGRS) always treated $^{222}$Rn as a source of background since it affects the indirect estimate of equivalent $^{238}$U concentration. In this work the AGRS method is used for the first time for quantifying the presence of $^{222}$Rn in the atmosphere and assessing its vertical profile. High statistics radiometric data acquired during an offshore survey are fitted as a superposition of a constant component due to the experimental setup background radioactivity plus a height dependent contribution due to cosmic radiation and atmospheric $^{222}$Rn. The refined statistical analysis provides not only a conclusive evidence of AGRS $^{222}$Rn detection but also a (0.96 $\pm$ 0.07) Bq/m$^{3}$ $^{222}$Rn concentration and a (1318 $\pm$ 22) m atmospheric layer depth fully compatible with literature data.Comment: 17 pages, 8 figures, 2 table

Thiol-gelatin-norbornene bioink for laser‐based high‐definition bioprinting

Two-photon polymerization (2PP) is a lithography-based 3D printing method allowing the fabrication of 3D structures with sub-micrometer resolution. This work focuses on the characterization of gelatin-norbornene (Gel-NB) bioinks which enables the embedding of cells via 2PP. The high reactivity of the thiol-ene system allows 2PP processing of cell-containing materials at remarkably high scanning speeds (1000 mm s(-1)) placing this technology in the domain of bioprinting. Atomic force microscopy results demonstrate that the indentation moduli of the produced hydrogel constructs can be adjusted in the 0.2-0.7 kPa range by controlling the 2PP processing parameters. Using this approach gradient 3D constructs are produced and the morphology of the embedded cells is observed in the course of 3 weeks. Furthermore, it is possible to tune the enzymatic degradation of the crosslinked bioink by varying the applied laser power. The 3D printed Gel-NB hydrogel constructs show exceptional biocompatibility, supported cell adhesion, and migration. Furthermore, cells maintain their proliferation capacity demonstrated by Ki-67 immunostaining. Moreover, the results demonstrate that direct embedding of cells provides uniform distribution and high cell loading independently of the pore size of the scaffold. The investigated photosensitive bioink enables high-definition bioprinting of well-defined constructs for long-term cell culture studies

Sensitivity analysis of damaged monumental structures: the example of S. Maria del Suffragio in L'Aquila

In Italy, which accounts for an impressive number of architectural heritage sites, a large part of the territory is subject to seismic risk. Nonetheless, also the two recent examples of the 2009 L'Aquila earthquake and 2012 Emilia earthquake confirmed and highlighted the vulnerability of cultural heritage structures to these types of events. In this paper the church of Santa Maria del Suffragio (Anime Sante) in L'Aquila is used as a benchmark for the experimental validation of a finite element model on the basis of the data gathered by the permanent structural health monitoring system installed on the building by IUAV in 2009. Structural health monitoring techniques have been largely applied to cultural Heritage buildings in recent times, mostly because of their non-destructive nature, and they have proven to be a valid tool in assessing the damage evolution and in characterising the global dynamic behaviour of the structure. In particular, a global sensitivity analysis technique has been applied to a finite element model. The model underwent a model updating procedure on the parameters chosen in the sensitivity analysis. The calibrated model is an invaluable tool in assessing the dynamic behaviour of the structure and may serve for several purpose

" Sensitivity analysis of damaged monumental structures: the example of S. Maria del Suffragio in L'Aquila "

In Italy, which accounts for an impressive number of architectural heritage sites, a large part of the territory is subject to seismic risk. Nonetheless, also the two recent examples of the 2009 L'Aquila earthquake and 2012 Emilia earthquake confirmed and highlighted the vulnerability of cultural heritage structures to these types of events. In this paper the church of Santa Maria del Suffragio (Anime Sante) in L'Aquila is used as a benchmark for the experimental validation of a finite element model on the basis of the data gathered by the permanent structural health monitoring system installed on the building by IUAV in 2009. Structural health monitoring techniques have been largely applied to cultural Heritage buildings in recent times, mostly because of their non-destructive nature, and they have proven to be a valid tool in assessing the damage evolution and in characterising the global dynamic behaviour of the structure. In particular, a global sensitivity analysis technique has been applied to a finite element model. The model underwent a model updating procedure on the parameters chosen in the sensitivity analysis. The calibrated model is an invaluable tool in assessing the dynamic behaviour of the structure and may serve for several purposes

Melatonin Attenuates Ischemic-like Cell Injury by Promoting Autophagosome Maturation via the Sirt1/FoxO1/Rab7 Axis in Hippocampal HT22 Cells and in Organotypic Cultures

Dysfunctional autophagy is linked to neuronal damage in ischemia/reperfusion injury. The Ras-related protein 7 (Rab7), a member of the Rab family of small GTPases, appears crucial for the progression of the autophagic flux, and its activity is strictly interconnected with the histone deacetylase Silent information regulator 1 (Sirt1) and transcription factor Forkhead box class O1 (FoxO1). The present study assessed the neuroprotective role of melatonin in the modulation of the Sirt1/FoxO1/Rab7 axis in HT22 cells and organotypic hippocampal cultures exposed to oxygen-glucose deprivation followed by reoxygenation (OGD/R). The results showed that melatonin re-established physiological levels of autophagy and reduced propidium iodide-positive cells, speeding up autophagosome (AP) maturation and increasing lysosomal activity. Our study revealed that melatonin modulates autophagic pathways, increasing the expression of both Rab7 and FoxO1 and restoring the Sirt1 expression affected by OGD/R. In addition, the Sirt1 inhibitor EX-527 significantly reduced Rab7, Sirt1, and FoxO1 expression, as well as autolysosomes formation, and blocked the neuroprotective effect of melatonin. Overall, our findings provide, for the first time, new insights into the neuroprotective role of melatonin against ischemic injury through the activation of the Sirt1/FoxO1/Rab7 axis

Azithromycin for sarcoidosis cough: an open label exploratory clinical trial

Background Chronic cough is a distressing symptom for many people with pulmonary sarcoidosis. Continuous treatment with a macrolide antibiotic may improve cough. We aimed to assess the potential efficacy of azithromycin in patients with sarcoidosis and self-reported cough.Methods We did a non-controlled, open label clinical trial of azithromycin 250 mg once daily for 3 months in patients with pulmonary sarcoidosis who reported a chronic cough. The primary outcome was number of coughs in 24 h. Secondary outcomes were cough visual analog scales and quality of life measured using the Leicester Cough Questionnaire and King's Sarcoidosis Questionnaire. Safety outcomes included QTc interval on ECG. Measurements were made at baseline and after one and 3 months of treatment.Results All 21 patients were white, median age 57 years, 9 males/12 females, median 3 years since diagnosis. Five were taking oral corticosteroids and none were taking other immunosuppressants. Twenty patients completed the trial. The median (range) number of coughs in 24 h was 228 (43–1950) at baseline, 122 (20–704) at 1 month, and 81 (16–414) at 3 months (p=0.002, Friedman's test). The median reduction in cough count at 3 months was 49.6%. There were improvements in all patient-reported outcomes. Azithromycin was well tolerated.Conclusion In a non-controlled open-label trial in people with sarcoidosis who reported a chronic cough, 3 months of treatment with azithromycin led to improvements in a range of cough metrics. Azithromycin should be tested as a treatment for sarcoidosis cough in a randomised placebo-controlled trial

Large variability of proanthocyanidin content and composition in sainfoin (onobrychis viciifolia)

Proanthocyanidins (PAs) in sainfoin (Onobrychis viciifolia Scop.) are of interest to ameliorate the sustainability of livestock production. However, sainfoin forage yield and PA concentrations, as well as their composition, require optimization. Individual plants of 27 sainfoin accessions from four continents were analyzed with LC-ESI-QqQ-MS/MS for PA concentrations and simple phenolic compounds. Large variability existed in PA concentrations (23.0–47.5 mg g–1 leaf dry matter (DM)), share of prodelphinidins (79–96%), and mean degree of polymerization (11–14) among, but also within, accessions. PAs were mainly located in leaves (26.8 mg g–1 DM), whereas stems had less PAs (7.8 mg g–1 DM). Overall, high-yielding plants had lower PA leaf concentrations (R2 = 0.16, P < 0.001) and fewer leaves (R2 = 0.66, P < 0.001). However, the results show that these two trade-offs between yield and bioactive PAs can be overcome

A bright megaelectronvolt emission line in γ\gamma-ray burst GRB 221009A

The highly variable and energetic pulsed emission of a long gamma-ray burst (GRB) is thought to originate from local, rapid dissipation of kinetic or magnetic energy within an ultra-relativistic jet launched by a newborn compact object, formed during the collapse of a massive star. The spectra of GRB pulses are best modelled by power-law segments, indicating the dominance of non-thermal radiation processes. Spectral lines in the X-ray and soft $\gamma$-ray regime for the afterglow have been searched for intensively, but never confirmed. No line features ever been identified in the high energy prompt emission. Here we report the discovery of a highly significant ($> 6 \sigma$) narrow emission feature at around $10$ MeV in the brightest ever GRB 221009A. By modelling its profile with a Gaussian, we find a roughly constant width $\sigma \sim 1$ MeV and temporal evolution both in energy ($\sim 12$ MeV to $\sim 6$ MeV) and luminosity ($\sim 10^{50}$ erg/s to $\sim 2 \times 10^{49}$ erg/s) over 80 seconds. We interpret this feature as a blue-shifted annihilation line of relatively cold ($k_\mathrm{B}T\ll m_\mathrm{e}c^2$) electron-positron pairs, which could have formed within the jet region where the brightest pulses of the GRB were produced. A detailed understanding of the conditions that can give rise to such a feature could shed light on the so far poorly understood GRB jet properties and energy dissipation mechanism.Comment: Submitte