Relation between spectral changes and the presence of the lower kHz QPO in the neutron-star low-mass X-ray binary 4U 1636-53

We fitted the $3-180$-keV spectrum of all the observations of the neutron-star low-mass X-ray binary 4U 1636$-$53 taken with the {\it Rossi X-ray Timing Explorer} using a model that includes a thermal Comptonisation component. We found that in the low-hard state the power-law index of this component, $\Gamma$, gradually increases as the source moves in the colour-colour diagram. When the source undergoes a transition from the hard to the soft state $\Gamma$ drops abruptly; once the source is in the soft state $\Gamma$ increases again and then decreases gradually as the source spectrum softens further. The changes in $\Gamma$, together with changes of the electron temperature, reflect changes of the optical depth in the corona. The lower kilohertz quasi-periodic oscillation (kHz QPO) in this source appears only in observations during the transition from the hard to the soft state, when the optical depth of the corona is high and changes depends strongly upon the position of the source in the colour-colour diagram. Our results are consistent with a scenario in which the lower kHz QPO reflects a global mode in the system that results from the resonance between, the disc and/or the neutron-star surface, and the Comptonising corona.Comment: 9 pages, 6 figures. Accepted for publication in MNRA

Impact of extracellular folic acid levels on oviductal gene expression

Folate plays a specific role as methyl donor for nucleotide synthesis and genomic methylation patterns, which in turn are important epigenetic determinants in gene expression. Previous studies have revealed the presence of folate in bovine oviductal fluid as well as the existence of a fine-tuned regulation of the gene expression of folate receptors and transporters in bovine oviduct epithelial cells (BOECs). However, the functional implications of folate in the oviduct remain unknown. The present study aimed to assess the effect of folic acid (FA) on expression levels of selected genes that potentially respond to the folate status in in vitro BOECs. To obtain an insight into the optimization of a culture system for assays, gene expression of folate receptors and transporters was compared between BOECs grown in monolayers and in suspension. The results showed that BOECs from isthmus and ampulla in suspension culture better preserved the region-dependent gene expression profile than in monolayers. Subsequently, BOECs from both anatomical regions were separately cultured in suspension for 24 h assaying different FA concentrations: I) TCM-199 (control); II) TCM-199 + 1 μM FA (similar to the oviduct concentration); III) TCM-199 + 10 μM FA and IV) TCM-199 + 100 μM FA. Expression analysis of genes related to important cellular processes including folate transport, DNA methylation, cell-cell interaction, antioxidant activity and signaling pathways was performed in BOECs using RT-qPCR. Our data demonstrated that addition of 1 μM FA did not affect mRNA levels of most genes analyzed. In contrast, BOECs cultured with 10 μM FA exhibited increased mRNA expression levels of genes involved in folate intake, DNA methylation and antioxidant protection. It is worth noting that at 100 μM FA, transcriptional response in BOECs mainly resulted in decreased mRNA levels of the majority of the genes assayed. Interestingly, cytotoxicity analysis showed a similar LDH activity in the culture media of the experimental groups, indicating that cell integrity was not affected by the FA concentrations assayed. In conclusion, our findings suggest that folate can affect BOECs, promoting changes in gene activity in a framework of functional readjustments in response to environmental conditions.Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto Superior de Investigaciones Biológicas. Grupo de Investigación y Desarrollo del Noroeste Argentino | Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas. Grupo de Investigación y Desarrollo del Noroeste Argentino; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; ArgentinaFil: Mansilla, Mariano J.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto Superior de Investigaciones Biológicas. Grupo de Investigación y Desarrollo del Noroeste Argentino | Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas. Grupo de Investigación y Desarrollo del Noroeste Argentino; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentin

Cancellation of vorticity in steady-state non-isentropic flows of complex fluids

In steady-state non-isentropic flows of perfect fluids there is always thermodynamic generation of vorticity when the difference between the product of the temperature with the gradient of the entropy and the gradient of total enthalpy is different from zero. We note that this property does not hold in general for complex fluids for which the prominent influence of the material substructure on the gross motion may cancel the thermodynamic vorticity. We indicate the explicit condition for this cancellation (topological transition from vortex sheet to shear flow) for general complex fluids described by coarse-grained order parameters and extended forms of Ginzburg-Landau energies. As a prominent sample case we treat first Korteweg's fluid, used commonly as a model of capillary motion or phase transitions characterized by diffused interfaces. Then we discuss general complex fluids. We show also that, when the entropy and the total enthalpy are constant throughout the flow, vorticity may be generated by the inhomogeneous character of the distribution of material substructures, and indicate the explicit condition for such a generation. We discuss also some aspects of unsteady motion and show that in two-dimensional flows of incompressible perfect complex fluids the vorticity is in general not conserved, due to a mechanism of transfer of energy between different levels.Comment: 12 page

The origin of UV/optical emission in the black hole low-mass X-ray binary Swift J1753.5-0127

The emission from the accreting black holes (BHs) in low-mass X-ray binaries (LMXBs) covers a broad energy band from radio to X-rays. Studying the correlations between emission in different energy bands during outbursts can provide valuable information about the accretion process. We analyse the simultaneous optical, ultraviolet (UV) and X-ray data of the BH-LMXB Swift J1753.5-0127 during its $\sim$ 12-year long outburst with the {\it Neil Gehrels Swift Observatory}. We find that the UV/optical and X-ray emission are strongly correlated during the hard states of the outburst. We fit the relation with a power-law function $F_{UV/optical} \propto F_{X}^{\beta}$ and find that the power-law index $\beta$ increases from $\sim$ 0.24 to $\sim$ 0.33 as the UV/optical wavelength decreases from $\sim$ 5400 \r{A} (V) to $\sim$ 2030 \r{A} (UVW2). We explore the possible reasons for this and suggest that in Swift J1753.5-0127 the UV/optical emission is dominated by a viscously heated accretion disc at large radii. We find that the data that deviate from the correlation correspond to the low-intensity peaks appeared in the X-ray band during the outburst, and suggest that these deviations are driven by the emission from the inner part of the accretion disc.Comment: 15 pages, 8 figures, 1 table, accepted by MNRA

Leaking method approach to surface transport in the Mediterranean Sea from a numerical ocean model

We use Lagrangian diagnostics (the leaking and the exchange methods) to characterize surface transport out of and between selected regions in the Western Mediterranean. Velocity fields are obtained from a numerical model. Residence times of water of Atlantic origin in the Algerian basin, with a strong seasonal dependence, are calculated. Exchange rates between these waters and the ones occupying the northern basin are also evaluated. At surface, northward transport is dominant, and involves filamental features and eddy structures that can be identified with the Algerian eddies. The impact on these results of the presence of small scale turbulent motions is evaluated by adding Lagrangian diffusion.Comment: 21 pages using the elsart style. Higher resolution figures available from http://www.imedea.uib.es/physdept/publications/showpaper_en.php?indice=119

In Vitro-In Vivo Translation of Lipid Nanoparticles for Hepatocellular siRNA Delivery

A significant challenge in the development of clinically viable siRNA delivery systems is a lack of in vitro–in vivo translatability: many delivery vehicles that are initially promising in cell culture do not retain efficacy in animals. Despite its importance, little information exists on the predictive nature of in vitro methodologies, most likely due to the cost and time associated with generating in vitro–in vivo data sets. Recently, high-throughput techniques have been developed that have allowed the examination of hundreds of lipid nanoparticle formulations for transfection efficiency in multiple experimental systems. The large resulting data set has allowed the development of correlations between in vitro and characterization data and in vivo efficacy for hepatocellular delivery vehicles. Consistency of formulation technique and the type of cell used for in vitro experiments was found to significantly affect correlations, with primary hepatocytes and HeLa cells yielding the most predictive data. Interestingly, in vitro data acquired using HeLa cells were more predictive of in vivo performance than mouse hepatoma Hepa1-6 cells. Of the characterization parameters, only siRNA entrapment efficiency was partially predictive of in vivo silencing potential, while zeta-potential and, surprisingly, nanoparticle size (when <300 nm) as measured by dynamic light scattering were not. These data provide guiding principles in the development of clinically viable siRNA delivery materials and have the potential to reduce experimental costs while improving the translation of materials into animals.Alnylam Pharmaceuticals (Firm)National Institutes of Health (U.S.) (Fellowship Award F32EB009623

GERONA. Asedios (18--)

Presenta una vista de la ciudadOrientado con lís, N. al SORelación del sistema defensivo y las derrotas sufridas indicadas mediante clave numéricaLeyenda explicativa con la historia de la ciudadIndica con flecha la dirección del río Te

Gerona

Presenta una vista de la ciudad de GeronaRelación de las baterías, fuertes, torres, campamento francés, baluartes, cuarteles así como los edificios más destacados de la ciudad y sus calles, indicada por clave numéricaSeñala el alcance de tiro de las bateríasEn nota se elogia a la ciudad que durante el sitio que duró seis meses, de Mayo a Diciembre, resistió y venció a las tropas francesas, mencionándose los generales franceses que se sucedieron a lo largo del mismo y el número de bombas que se lanzaronProcede de la colección de D. Manuel Rico y SinobasExisten dos ejemplares facsímiles en el Centro de Documentación del Ministerio de Defens