Sheep corneal endothelium morphology : evaluation with trypan blue and alizarin red

Background: The endothelium is a layer fundamental to maintaining corneal transparency. In ophthalmology, sheep eyes have been used as a model in research related to corneal transplantation. Different techniques have been used to evaluate the corneal endothelium. Concerning vital dyes, corneal endothelial cell analyses have not yet been studied in ovines. The purpose of the present study was to evaluate the morphology of endothelial cells from different regions of the cornea of sheep after staining with alizarin red and trypan blue using an optical microscope. Materials, Methods & Results: Twenty healthy eyes of 10 male sheep obtained from a licensed commercial slaughterhouse were studied. The study was approved by the Research Committee of the Faculty of Veterinary at UFRGS and followed the ethical standards of the Association for Research in Vision and Ophthalmology (ARVO). Immediately after the slaughter, the eyes were enucleated and underwent eye examination. The corneal endothelium was stained with trypan blue and alizarin red and examined and photographed using an optical microscope. The central, superior, inferior, nasal and temporal areas of the cornea were evaluated for cell morphology. Data were compared by t-tests. Differences were considered statistically significant at P < 0.05. Immediately after staining the corneal endothelium, it was possible to examine with an optical microscope, obtain images and analyse the shape of endothelial cells from all regions of the sheep cornea. Polygonal, uniform and continuous cells were observed in all samples studied. Considering all the corneas analysed, cells with 6 sides (75.11%), 5 sides (12.76%) and 4 sides (12.12%) were found. In the central region of the cornea 75.91% of cells with 6 sides, 12.6% of cells with 5 sides and 11.48% with 7 sides were found. In the superior region of the cornea 76.07% of cells with 6 sides, 13.25% with 5 sides and 10.68% with 7 sides were found. In the lower region were found 74.72% of cells with 6 sides, 13% with 5 sides and 12.27% with 7 sides. In the temporal region, 74.14% were 6-sided cells, 11.42% had 5 sides, and 14.43% had 7 sides. Furthermore, in the nasal region, 74.72% of the cells had 6 sides, 13.54% had 5 sides, and 11.73% had 7 sides. No significant differences were found between cell morphology in all corneal regions evaluated. In addition, no significant difference was found when comparing the right eye with the left eye. Discussion: Different methods are used for the analysis of corneal endothelium. For ex vivo research optical microscopy after endothelial staining is an alternative low-cost technique that allows the analysis of all regions of the cornea. Quan¬titative analyses must characterise the endothelial parameters of the different species. The analysis of the morphology of corneal endothelium with an optic microscope after staining with alizarin red has been described as an effective, rapid and cost-efficient method, since this dye blends with the borated cells, allowing identification. In the present study, using optical microscopy and coloration with alizarin red it was possible to explore and obtain images of the ovine endothelium of all regions of the cornea. In the current study, the endothelium had a predominance of cells will 6 sides in all regions studied. This study allowed us to obtain images of the endothelium as well as quantitative data on the morphology of the different regions of the sheep cornea. This study demonstrated that morphology did not differ between the central and peripheral regions. The findings of this study represent a further source of reproducible data that should be considered when using sheep cornea as ex vivo model for experimental research

Sheep Corneal Endothelium Morphology - Evaluation with Trypan Blue and Alizarin Red

Background: The endothelium is a layer fundamental to maintaining corneal transparency. In ophthalmology, sheep eyes have been used as a model in research related to corneal transplantation. Different techniques have been used to evaluate the corneal endothelium. Concerning vital dyes, corneal endothelial cell analyses have not yet been studied in ovines. The purpose of the present study was to evaluate the morphology of endothelial cells from different regions of the cornea of sheep after staining with alizarin red and trypan blue using an optical microscope. Materials, Methods &amp; Results: Twenty healthy eyes of 10 male sheep obtained from a licensed commercial slaughterhouse were studied. The study was approved by the Research Committee of the Faculty of Veterinary at UFRGS and followed the ethical standards of the Association for Research in Vision and Ophthalmology (ARVO). Immediately after the slaughter, the eyes were enucleated and underwent eye examination. The corneal endothelium was stained with trypan blue and alizarin red and examined and photographed using an optical microscope. The central, superior, inferior, nasal and temporal areas of the cornea were evaluated for cell morphology. Data were compared by t-tests. Differences were considered statistically significant at P &lt; 0.05. Immediately after staining the corneal endothelium, it was possible to examine with an optical microscope, obtain images and analyse the shape of endothelial cells from all regions of the sheep cornea. Polygonal, uniform and continuous cells were observed in all samples studied. Considering all the corneas analysed, cells with 6 sides (75.11%), 5 sides (12.76%) and 4 sides (12.12%) were found. In the central region of the cornea 75.91% of cells with 6 sides, 12.6% of cells with 5 sides and 11.48% with 7 sides were found. In the superior region of the cornea 76.07% of cells with 6 sides, 13.25% with 5 sides and 10.68% with 7 sides were found. In the lower region were found 74.72% of cells with 6 sides, 13% with 5 sides and 12.27% with 7 sides. In the temporal region, 74.14% were 6-sided cells, 11.42% had 5 sides, and 14.43% had 7 sides. Furthermore, in the nasal region, 74.72% of the cells had 6 sides, 13.54% had 5 sides, and 11.73% had 7 sides. No significant differences were found between cell morphology in all corneal regions evaluated. In addition, no significant difference was found when comparing the right eye with the left eye. Discussion: Different methods are used for the analysis of corneal endothelium. For ex vivo research optical microscopy after endothelial staining is an alternative low-cost technique that allows the analysis of all regions of the cornea. Quantitative analyses must characterise the endothelial parameters of the different species. The analysis of the morphology of corneal endothelium with an optic microscope after staining with alizarin red has been described as an effective, rapid and cost-efficient method, since this dye blends with the borated cells, allowing identification. In the present study, using optical microscopy and coloration with alizarin red it was possible to explore and obtain images of the ovine endothelium of all regions of the cornea. In the current study, the endothelium had a predominance of cells will 6 sides in all regions studied. This study allowed us to obtain images of the endothelium as well as quantitative data on the morphology of the different regions of the sheep cornea. This study demonstrated that morphology did not differ between the central and peripheral regions. The findings of this study represent a further source of reproducible data that should be considered when using sheep cornea as ex vivo model for experimental research. Keywords: ovine, endothelial cells, ex vivo model, vital staining, hexagonality

Morphology of bovine corneal endothelial cells obtained with alizarin red and optical microscopy

The aim of this study was to determine the endothelial cell morphology in the different regions of healthy bovine corneal endothelium using optical microscopy. Twenty eyeballs from 10 male Brangus cattle, aged 24 months, were studied. The corneal endothelium was stained with the vital dye alizarin red and then examined with an optical microscope and photographed. Thirty endothelial cells from each corneal region were included in the analysis. Endothelial cell morphology was analysed in the central, superior, inferior, lateral and medial regions of the cornea. Comparisons between regions were performed using repeated measures analysis of variance (ANOVA). Differences were considered statistically significant at P < 0.05. Normal endothelial cells were mainly hexagonal (83.7%), pentagonal (7.45%) and heptagonal (8.8%), with a minimal number of cells of other shapes present. No statistical differences were observed in the endothelial cell morphology when comparing different regions of the cornea. Regarding endothelial cell morphology, there were no differences between the corneal regions

Uveíte em cães : revisão bibliográfica

A úvea é constituída pela íris, corpo ciliar e coróide. A uveíte é a inflamação da úvea e frequentemente acomete cães, sendo esta uma das principais inflamações ocasionadas por doenças infecciosas nestes animais. Com base nisto, é de extrema importância que se estabeleça o diagnóstico correto e a pesquisa da doença infecciosa que serviu de agravante para a inflamação. Objetiva-se com esta revisão bibliográfica abordar principalmente a etiopatogenia, o diagnóstico, o tratamento e as sequelas da uveíte em cães.The uvea is represented by the iris, ciliary body and choroid. Uveitis, on the other hand, consists of inflammation of the uvea and dogs are commonly affected, being this one of the main inflammations caused by infectious diseases in these animals. Based on this, it is extremely important to establish the correct diagnosis and research of the infectious disease that has aggravated the inflammation. This work is a bibliographical review that emphasizes the etiopathogenesis of uveitis, its causes, complications, treatments and sequelae

Uveíte em cães : revisão bibliográfica

A úvea é constituída pela íris, corpo ciliar e coróide. A uveíte é a inflamação da úvea e frequentemente acomete cães, sendo esta uma das principais inflamações ocasionadas por doenças infecciosas nestes animais. Com base nisto, é de extrema importância que se estabeleça o diagnóstico correto e a pesquisa da doença infecciosa que serviu de agravante para a inflamação. Objetiva-se com esta revisão bibliográfica abordar principalmente a etiopatogenia, o diagnóstico, o tratamento e as sequelas da uveíte em cães.The uvea is represented by the iris, ciliary body and choroid. Uveitis, on the other hand, consists of inflammation of the uvea and dogs are commonly affected, being this one of the main inflammations caused by infectious diseases in these animals. Based on this, it is extremely important to establish the correct diagnosis and research of the infectious disease that has aggravated the inflammation. This work is a bibliographical review that emphasizes the etiopathogenesis of uveitis, its causes, complications, treatments and sequelae

Avaliação do endotélio da córnea de bovinos (Bos taurus) utilizando a microscopia especular de contato : estudo ex vivo

O endotélio constitui a camada posterior da córnea, é formado por uma camada única de células poligonais em um padrão mosaico organizado, sendo essa estrutura fundamental para manter a córnea transparente. Devido às variações nos parâmetros endoteliais da córnea entre as diferentes espécies o conhecimento dos parâmetros normais de cada espécie é fundamental. Objetivou-se avaliar o endotélio da córnea de bovinos utilizando o microscópio especular de contato. Procedimentos: Foram avaliados 20 bulbos oculares de 10 bovinos (Bos taurus), machos, com a idade de 24 meses da raça brangus. A microscopia especular de contato foi realizada na área central da córnea. Resultados: A densidade celular média na área central foi de 1277,0 células/mm² e a porcentagem de células hexagonais foi de 74,3 %. Não foram observadas diferenças na densidade celular endotelial e na hexagonalidade entre os olhos esquerdo e direito. Conclusão: Com a microscopia especular de contato foi possível analisar e mensurar os parâmetros endoteliais da córnea de bovinos. Os dados obtidos servirão de referência para análise do endotélio da córnea de bovinos.The corneal endothelium is the posterior layer of the cornea, consisting of a single layer of polygonal cells in an organized mosaic pattern, wich is essential for maintaining corneal transparency. Due to variations in corneal endothelial parameters among different species, knowledge of the normal parameters of each species is crucial. The objective of this study was to evaluate the corneal endothelium of bovines using a contact specular microscope. Procedures: Twenty eyeballs from 10 male Brangus bovines (Bos taurus), aged 24 months, were evaluated. Contact specular microscopy was performed on the central area of the cornea. Results: The mean cell density in the central area was 1277,0 cells/mm² and the percentage of hexagonal cells was 74.3%. No differences in endothelial cell density and hexagonality were observed between the left and right eyes. Conclusion: Contact specular allowed for the analysis and measurement of corneal endothelial parameters in bovines. The obtained data will serve as a reference for the analysis of corneal endothelium in bovines

Sheep Corneal Endothelium Morphology - Evaluation with Trypan Blue and Alizarin Red

Background: The endothelium is a layer fundamental to maintaining corneal transparency. In ophthalmology, sheep eyes have been used as a model in research related to corneal transplantation. Different techniques have been used to evaluate the corneal endothelium. Concerning vital dyes, corneal endothelial cell analyses have not yet been studied in ovines. The purpose of the present study was to evaluate the morphology of endothelial cells from different regions of the cornea of sheep after staining with alizarin red and trypan blue using an optical microscope. Materials, Methods &amp; Results: Twenty healthy eyes of 10 male sheep obtained from a licensed commercial slaughterhouse were studied. The study was approved by the Research Committee of the Faculty of Veterinary at UFRGS and followed the ethical standards of the Association for Research in Vision and Ophthalmology (ARVO). Immediately after the slaughter, the eyes were enucleated and underwent eye examination. The corneal endothelium was stained with trypan blue and alizarin red and examined and photographed using an optical microscope. The central, superior, inferior, nasal and temporal areas of the cornea were evaluated for cell morphology. Data were compared by t-tests. Differences were considered statistically significant at P &lt; 0.05. Immediately after staining the corneal endothelium, it was possible to examine with an optical microscope, obtain images and analyse the shape of endothelial cells from all regions of the sheep cornea. Polygonal, uniform and continuous cells were observed in all samples studied. Considering all the corneas analysed, cells with 6 sides (75.11%), 5 sides (12.76%) and 4 sides (12.12%) were found. In the central region of the cornea 75.91% of cells with 6 sides, 12.6% of cells with 5 sides and 11.48% with 7 sides were found. In the superior region of the cornea 76.07% of cells with 6 sides, 13.25% with 5 sides and 10.68% with 7 sides were found. In the lower region were found 74.72% of cells with 6 sides, 13% with 5 sides and 12.27% with 7 sides. In the temporal region, 74.14% were 6-sided cells, 11.42% had 5 sides, and 14.43% had 7 sides. Furthermore, in the nasal region, 74.72% of the cells had 6 sides, 13.54% had 5 sides, and 11.73% had 7 sides. No significant differences were found between cell morphology in all corneal regions evaluated. In addition, no significant difference was found when comparing the right eye with the left eye. Discussion: Different methods are used for the analysis of corneal endothelium. For ex vivo research optical microscopy after endothelial staining is an alternative low-cost technique that allows the analysis of all regions of the cornea. Quantitative analyses must characterise the endothelial parameters of the different species. The analysis of the morphology of corneal endothelium with an optic microscope after staining with alizarin red has been described as an effective, rapid and cost-efficient method, since this dye blends with the borated cells, allowing identification. In the present study, using optical microscopy and coloration with alizarin red it was possible to explore and obtain images of the ovine endothelium of all regions of the cornea. In the current study, the endothelium had a predominance of cells will 6 sides in all regions studied. This study allowed us to obtain images of the endothelium as well as quantitative data on the morphology of the different regions of the sheep cornea. This study demonstrated that morphology did not differ between the central and peripheral regions. The findings of this study represent a further source of reproducible data that should be considered when using sheep cornea as ex vivo model for experimental research. Keywords: ovine, endothelial cells, ex vivo model, vital staining, hexagonality