Methods for the determination of organochlorine pesticide residues in human serum

The effectiveness of solid-phase extraction with Florisil for the determination of 12 organochlorine pesticide residues from human serum was examined. Recoveries greater than 84% and coefficients of variation better than 19% were obtained. Others methods, such as column partition and matrix solid-phase dispersion, were compared. The better method provides quantification limits ranging from 1.08 [mu]g/l for [gamma]-HCH and 37.5 [mu]g/l for p,p'-DDT when capillary gas-liquid chromatography with electron-capture detection is used for the final determination.http://www.sciencedirect.com/science/article/B6TG9-3TWXX3J-J/1/b7804f2ca479d02daf6147329c4f29c

Determination of nitrofurans in animal feeds by liquid chromatography-UV photodiode array detection and liquid chromatography-ionspray tandem mass spectrometry

Within the EU, the use of nitrofurans is prohibited in food production animals. For this reason detection of these compounds in feedingstuffs, at whatever limit, constitutes an offence under EU legislation. This detection generally involves the use of analytical methods with limits of quantification lowers than 1 mg kg-1. These procedures are unsuitable for the detection and confirmation of trace amounts of nitrofurans in feedingstuffs due to contamination. It is well known that very low concentrations of these compounds can be the source of residues of nitrofuran metabolites in meat and other edible products obtained from animals consuming the contaminated feed. The present multi-compound method was capable of measuring very low concentrations of nitrofurantoin (NFT), nitrofurazone (NFZ), furazolidone (FZD) and furaltadone (FTD) in animal feed using nifuroxazide (NXZ) as internal standard. Following ethyl acetate extraction at mild alkaline conditions and purification on NH2 column, the nitrofurans are determined using liquid chromatography with photodiode-array detection (LC-DAD). It was observed a CC[alpha] ranged from 50 to 100 [mu]g kg-1. The liquid chromatography-tandem mass spectrometric (LC-MS/MS) procedure was used to confirm the identity of the suspected presence of any of the nitrofuran compounds.http://www.sciencedirect.com/science/article/B6TF4-4MFJ2HW-2/1/a316f0a51003ac6889ccac0f4af2813

Control of the illegal use of clenbuterol in bovine production

This study is based on a plan of collecting different matrices (hair, eye, muscle, liver and kidney) in order to define a strategy for the control of the illegal use of clenbuterol in bovine production. Of all matrices utilised, hair is recommended for the analytical control of clenbuterol in living animals, due to its being permanently available and easy to collect. The eye, or rather the retina, is the matrix which gives the most trustworthy result, after the animal slaughter, and the one that best helps in the determination of the illegal use of clenbuterol in a perspective of gradual food safety improvement.http://www.sciencedirect.com/science/article/B6TGX-487KS9W-1/1/a2e3907888615efd8c5cc06f89154d2

Determination of the furaltadone metabolite 5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ) using liquid chromatography coupled to electrospray tandem mass spectrometry during the nitrofuran crisis in Portugal

Abstract The use of nitrofuran veterinary drugs as antibacterial compounds in food-producing animals has been banned in the EU since 1995. As nitrofurans are extensive and rapidly metabolized, control of their illegal use in animal production must be done in edible tissues by LC-MS/MS analysis in order to determine persistent tissue-bound metabolites. The introduction during 2002 of the multi-residue detection of nitrofuran tissue-bound metabolites by LC-MS/MS for nitrofuran control in Portuguese Residues Monitoring Plan, revealed the presence of 5-morpholinomethyl-3-amino-2-oxozolidinone (AMOZ), the bound residue of furaltadone, in a large number of samples, namely in meat poultry samples. From the 226 analysed samples in the last 4 months of 2002, 78 were non-compliant due to the presence of AMOZ (61 broilers, 11 turkeys, 5 quails and 1 pig). In this context, the aim of this paper is to describe the analytical data obtained on meat samples collected from various animal species under official Portuguese control for nitrofuran drug residues during the so-called “Portuguese nitrofuran crisis”

Production of “in house” reference materials for ELISA screening of bovine urine and liver samples for clenbuterol

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