Passive transfer status and growth performance in newborn buffalo calves allowed to nurse the dam

The objective of this study was to evaluate the effect of passive transfer status, determined by measuring serum IgG concentration 24 hours after parturition, on growth performance in buffalo calves fed milk replacer or allowed to nurse the dam during the first month of life. Experiment consisted of 24 healthy buffalo calves from birth to 30 days old. Significant quadratic associations were detected between serum IgG concentration 24 hours after birth and day-30 weight (P < 0.05; R2 = 0.62) and between serum IgG concentration 24 hours after birth and the mean daily gain from birth to day 30 (P < 0.01; R2 = 0.74) in buffalo calves allowed to nurse the dam. No significant association was detected between serum IgG concentration 24 hours after birth and measures of growth performance in buffalo calves fed milk replacer. Results indicated that passive transfer status, determined as serum IgG concentration 24 hours after birth, was a significant source of variation in growth performance when buffalo calves nursed the dam. Maximizing passive transfer of immunity by allowing buffalo calves to nurse the dam increased the growth performance during the first month of life

Growth hormone response to arginine test distinguishes multiple system atrophyfrom Parkinson's disease and idiopathic late-onset cerebellar ataxia

OBJECTIVE: Multiple system atrophy (MSA) is difficult to distinguish from idiopathic Parkinson's disease (PD) and idiopathic late-onset cerebellar ataxia (ILOCA). This study aimed to evaluate GH response to three different GH stimulation tests in order to establish a reliable test to differentiate these degenerative disorders. DESIGN: Twelve patients with MSA, 10 with PD, eight with ILOCA and 30 healthy controls entered the study. They were submitted to clonidine, arginine, and GH-releasing-hormone (GHRH) + arginine tests in a random manner on three different nonconsecutive days. The peak serum GH response was used as a primary variable for analysis of stimulation tests. By ROC analysis, the optimum cut-off level was considered as the cut-off with the maximal sum of sensitivity and specificity. RESULTS: After clonidine administration, GH peak was significantly lower in patients with MSA than in those with ILOCA (P < 0.05) and in the controls (P < 0.001). At the optimum cut-off level of 5 mU/l, the clonidine test distinguished patients with MSA from those with PD with a sensitivity and specificity of 78%. Moreover, this test distinguished patients with MSA from those with ILOCA with a sensitivity of 100% and a specificity of 75% at a cut-off level of 5 mU/l, and with a sensitivity of 75% and a specificity of 100% at the cut-off level of 7.6 mU/l. After arginine administration, the GH peak was significantly lower in patients with MSA than in those with ILOCA (P = 0.001) and in controls (P < 0.001). At the optimum cut-off level of 5 mU/l, the arginine test distinguished patients with MSA from those with PD with a sensitivity and a specificity of 100%. At a GH peak cut-off value of 3.6 mU/l the arginine test distinguished patients with MSA from those with ILOCA with a sensitivity and specificity of 100%. After GHRH + arginine administration, a significant GH increase was found in all groups of patients and controls. CONCLUSIONS: The GH response to arginine administration is impaired in MSA. Therefore, the arginine test showed the highest diagnostic accuracy to distinguish MSA from both PD and ILOCA, and could be used in the clinical practice of these neurodegenerative diseases

Simulation and comparison of coils for Hyperpolarized 13C MRS cardiac metabolism studies in pigs

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ANALISI DELL’INDICE DI INTERAZIONE BAMBINO-PETS IN FATTORIA ZOOANTROPOLOGICA: STUDIO PILOTA

INTRODUZIONE - La didattica applicata alla zooantropologia prevede un’attuazione in campo educativo e pedagogico dei principi zooantropologici; essa si prefigge un duplice obiettivo: di ridurre il gap relazionale tra animali e giovani generazioni e di recuperare i contenuti e le valenze della relazione, al fine dell’utilizzo pedagogico ed educativo. Nasce un nuovo modello educativo che pone al centro delle strutture pedagogiche l’animale, non più come oggetto da sfruttare, ma come soggetto che aiuti il bambino nella sua crescita educativa (1,2). In quest’ottica generale, la Fattoria Zooantropologica sembra essere un buon sistema in grado di permettere la “fruizione” da parte di bambini e\o soggetti con disabilità di questo modello relazionale e di consentire, altresì, attività e terapie assistite con gli animali (3,4). La Fattoria Zooantropologica offre l’opportunità di attivare la relazione reciproca in un ambiente nuovo, attraente e confortevole tra il fruitore e l’animale che giova di un habitat naturale e familiare. L’esperienza diretta permette ai bambini di usare tutti i loro sensi per imparare: più i bambini vedono, toccano, odorano, sentono, gustano, più imparano (5,6). Partendo da questi presupposti, lo studio si è posto l’obiettivo di effettuare una valutazione del gradimento delle differenti specie animali, attraverso l’analisi degli indici di interazione dei bambini rispetto agli animali presenti in fattoria, al fine di fornire delle linee guida nella scelta del pet in relazione ad una amplificazione delle risposte cognitive ed emozionali del bambino

Continuous monitoring of natural CO2 emissions near Rome: lessons for low-level CO2 leakage detection

Continuous monitoring has been carried out at a fluvial flood-plain site near Rome for over a year. There is a mix of biogenic CO2 and deep geogenic CO2 at the site at relatively low concentrations and fluxes compared with other natural CO2 seepage sites studied previously. Factors such as temperature and soil moisture clearly affect the CO2 concentration and flux and seasonal and diurnal influences are apparent. Statistical approaches are being used to try to define these relationships and separate out the two gas components, which would be necessary in any quantification of leakage from CO2 storage

Choosing wisely: practical considerations on treatment efficacy and safety of asthma in the elderly.

The prevalence of asthma in the most advanced ages is similar to that of younger ages. However, the concept that older individuals may suffer from allergic asthma has been largely denied in the past, and a common belief attributes to asthma the definition of "rare" disease. Indeed, asthma in the elderly is often underdiagnosed or diagnosed as COPD, thus leading to undertreatment of improper treatment. This is also due to the heterogeneity of clinical and functional presentations of geriatric asthma, including the partial loss of reversibility and the lower occurrence of the allergic component in this age range. The older asthmatic patients are also characterized the coexistence of comorbid conditions that, in conjunction with age-associated structural and functional changes of the lung, may contribute to complicate the management of asthma. The current review addresses the main issues related to the management of allergic asthma in the geriatric age. In particular, the paper aims at revising current pharmacological and non pharmacological treatments for allergic asthmatics of advanced ages, primarily focusing on their safety and efficacy, although most behaviors are an arbitrary extrapolation of what has been tested in young ages. In fact, age has always represented an exclusion criterion for eligibility to clinical trials. Experimental studies and real life observations specifically testing the efficacy and safety of therapeutic approaches in allergic asthma in the elderly are urgently needed

Polydatin, a natural precursor of resveratrol, induces cell cycle arrest and differentiation of human colorectal Caco-2 cell.

Background: Human colon adenocarcinoma cells are resistant to chemotherapeutic agents, such as anthracyclines, that induce death by increasing the reactive oxygen species. A number of studies have been focused on chemo-preventive use of resveratrol as antioxidant against cardiovascular diseases, aging and cancer. While resveratrol cytotoxic action was due to its pro-oxidant properties. In this study, we investigate whether the Resveratrol (trans-3,5,49-trihydroxystilbene) and its natural precursor Polydatin (resveratrol-3-O-b-mono- D-glucoside, the glycoside form of resveratrol) combination, might have a cooperative antitumor effect on either growing or differentiated human adenocarcinoma colon cancer cells. Methods: The polydatin and resveratrol pharmacological interaction was evaluated in vitro on growing and differentiated Caco-2 cell lines by median drug effect analysis calculating a combination index with CalcuSyn software. We have selected a synergistic combination and we have evaluated its effect on the biological and molecular mechanisms of cell death. Results: Simultaneous exposure to polydatin and resveratrol produced synergistic antiproliferative effects compared with single compound treatment. We demonstrated that polydatin alone or in combination with resveratrol at 3:1 molar ratio synergistically modulated oxidative stress, cell cycle, differentiation and apoptosis. Worthy of note treatment with polydatin induced a nuclear localization and decreased expression of heat shock protein 27, and vimentin redistributed within the cell. Conclusions: From morphological, and biochemical outcome we obtained evidences that polydatin induced a transition from a proliferative morphology to cell-specific differentiated structures and caused human CaCo-2 cell death by induction of apoptosis. Our data suggest the potential use of polydatin in combination chemotherapy for human colon cancer