The Effect of Trehalose in Inhibiting Liver Damage via The Il-6 Pathway in Old Mice

Trehalose is a non-reducing disaccharide consisting of two glucose molecules linked by a 1–1 glycosidic bond. Trehalose can play a role in organ detoxification, antioxidant enzyme activity, reducing lipid peroxidation and reducing the secretion of inflammatory factors TNF-, IL-1β, Il-6, thereby inhibiting liver damage. Because of its role, this study aims to determine the effect of trehalose administration on liver damage through the IL-6 expression pathway with experimental post-test design with control group design. This research was conducted using samples of old rats, namely Wistar rats (Rattus novergicus). The old rats used were 21 male species which were then divided into 3 groups, namely the old control group, the old rat group that was given sucrose, and the old rat group that was given trehalose. Then observed for 8 weeks, namely in March - May 2021 at the Faculty of Medicine, Hasanuddin University Makassar. The results obtained from the 3 groups showed a significant value that the administration of trehalose sugar in elderly mice had an effect on reducing the inflammatory factor IL-6 because it activated autophagy in macrophages thereby reducing cytokine production and vascular inflammation so as to be able to restore all aging mice to a level like observed in young mice. It is also known that aged rat liver, which is characterized by ER UPR activation and inflammatory signaling, decreases nicotinamide and UDP-N-acetyl-hexosamines, and reduces proteasome activity. Therefore, trehalose can be an effective therapeutic strategy against age-related disorders of proteostasis in the liver

The effect of family education on the fulfillment of family health tasks in handling case of hypertension post earthquake in Sigi regency

The role of a good family is indispensable in handling cases of hypertension, direct involvement of family members is a form of support so that hypertension care management can run well. The purpose of this study was to determine whether there was an effect of family education on the fulfillment of family health tasks in handling hypertension cases after the earthquake in Sigi Regency. This research was conducted from December 2018 to March 2019 using an analytical survey pre and post-test design with a sample of 30 respondents from families with hypertension. Sampling using non-probability sampling technique with a purposive sampling approach. The data were then analyzed using the McNenmar test. There is a significant difference between blood pressure before education and after family education was conducted (? value = 0.001), while at the level of family knowledge, there was a significant difference before and after family education was carried out on the fulfillment of family health tasks in handling hypertension cases after the earthquake in Sigi district. There is an effect of family education on reducing blood pressure in patients with hypertension after the earthquake that occurred in Sigi Regency

Effect of Dangke consumption on body weight, blood glucose, and total cholesterol in rats subjected to high-fat and fructose diet

Obesity occurs due to an imbalance in caloric intake, leading to excess fat accumulation in adipose tissue. This condition is associated with hyperglycemia and abnormal lipid levels. Dangke, a traditional Indonesian cheese, contains lactic acid bacteria and bioactive peptides known for their health benefits. This study aims to assess the effects of dangke consumption on body weight, glucose, and total cholesterol levels in rats subjected to a high-fat and fructose diet (HFFD). This research employed an experimental laboratory design. Twenty-four Sprague-Dawley rats, with weights ranging from 100 to 200 g, were divided into six groups, each consisting of four rats. Group 1 (ND) (received a normal diet. Group 2 (HFFD) was provided with a high-fat and fructose diet)

Viability, Migration Rate, and mRNA Expression of GLUT5, GLUT7, GLUT11 in WiDr Colorectal Cancer Cell Line

BACKGROUND: Insufficient glucose levels in colorectal cancer (CRC) patients leads to a condition where fructose might become an alternative source for cells proliferation, but the role of fructose or fructose-glucose combinations in development of CRC has not been elucidated well. In this study, the effect of fructose-glucose variations on viability, migration, and glucose transporter (GLUT)5, GLUT7, GLUT11 mRNA expressions in WiDr CRC cell line were examined.METHODS: Cells were treated with varying ratios of fructose-glucose (F100%; F75%:G25%; F50%:G50%; F25%:G75%; G100%; F: Fructose, G: Glucose). Untreated cells (F0:G0) were used as cell control. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used for cell viability test, scratch assay was used to examine the cell migration, and quantitative polymerase chain reaction (qPCR) was performed to examine mRNA expressions. Data were analyzed using one-way analysis of variance (ANOVA) and followed with Tukey's post-hoc test, with p<0.05 consideres as significant.RESULTS: Fructose-glucose combinations and glucose 100% significantly increased the cell viability compared to control (p<0.05). All treatment groups showed a significant increase in cell migration compared to control (p=0.000). Only GLUT7 and GLUT11 expressions in the G100% group were significantly different compared to the control (p=0.000). GLUT7 and GLUT11 expressions were also significantly different in F100% and F50%:G50% treatments compared to G100% (p=0.000).CONCLUSION: Taken together, fructose might play important role in cell migration. However, in cell viability, combination with glucose could increase fructose's effect. Fructose might not affect the mRNA expressions of GLUT5, GLUT7 and GLUT11.KEYWORDS: GLUT5, GLUT7, GLUT11, fructose transporter, colorectal cancer, WiD