BIOQUIMICO PLASMÁTICO DE CÃES: EFEITOS DOS DIFERENTES ANTICOAGULANTES EM COMPARAÇÃO COM O SORO

Na rotina clínica veterinária são utilizados diferentes tipos de anticoagulantes, escolhidos de acordo com o exame a ser solicitado. Animais agitados, agressivos, de difícil contenção e de pequeno porte podem ser fatores limitantes quando se deseja aliquotar a amostra em tubos com diferentes anticoagulantes. Assim, esse trabalho teve como objetivo avaliar os efeitos dos anticoagulantes EDTA, Citrato de Sódio e Fluoreto associado ao EDTA, nos resultados dos exames bioquímicos de rotina realizados em cães e compará-los com o soro. Verificou-se que o plasma obtido com o anticoagulante EDTA resultou em alterações nas dosagens de glicose, colesterol e cálcio. Amostras obtidas com Citrato de Sódio tiveram resultados inferiores ao do soro em todos os parâmetros avaliados, com exceção da ALT. O anticoagulante Fluoreto associado ao EDTA, apenas nas dosagens de uréia e glicose, não resultou em alterações estatísticas quando comparado ao soro

Maxwell's Equations in the Myers-Perry Geometry

We demonstrate separability of the Maxwell's equations in the Myers-Perry-(A)dS geometry and derive explicit solutions for various polarizations. Application of our construction to the four-dimensional Kerr black hole leads to a new ansatz for the Maxwell field which has significant advantages over the previously known parameterization.Comment: 94 pages. V2: Appendix F added, typos fixe

BIOQUIMICO PLASMÁTICO DE CÃES: EFEITOS DOS DIFERENTES ANTICOAGULANTES EM COMPARAÇÃO COM O SORO

Different types of anticoagulants are used in the routine of a veterinary clinic, which are chosen based on the exam to be performed. Agitated, aggressive, of hard contention and small size animals can be a limiting factor when it is wished to separate samples into tubes with different anticoagulants. Therefore, this study had as the objective evaluate the effects of anticoagulants: EDTA, sodium citrate and sodium fluorite associated with EDTA, in the results of routine plasma biochemistry performed in dogs and compare it with serum. Blood samples of 10 clinically healthy dogs were collected and placed in different tubes with each anticoagulant and one without to obtain serum. Albumin, alanine aminotransferase (ALT), calcium, total cholesterol, creatine, phosphorus, glucose, total protein (PT) and urea concentrations were measured by spectophotometric. It was found that plasma obtained with EDTA anticoagulant resulted in dosage alterations for glucose, cholesterol and calcium. Samples obtained with sodium citrate presented results lower than serum in all analyzed parameters, except for ALT. The anticoagulant sodium 34 Colloquium Agrariae, v. 11, n.1, Jan-Jun. 2015, p.33-41. DOI: 10.5747/ca.2015.v11.n1.a116 fluorite with EDTA only didn’t result in statistical differences when compared to serum in the parameters of urea and glucose. Thus, serum is the most trustworthy sample to determine the biochemical profile of dogs, however some biochemical’s can be performed with plasma with EDTA such as creatine, ALT, urea, albumin, PT and phosphorus

FREQUÊNCIA DE ANTICORPOS ANTI-TOXOPLASMA GONDII EM CÃES COM SINAIS CLÍNICOS COMPATÍVEIS COM TOXOPLASMOSE

O objetivo deste trabalho foi detectar anticorposcontra T. gondii emcãescom sinais clínicoscompatíveis com toxoplasmose atendidos em um Hospital Veterinário. Foram submetidos a sorologia 598 cães no período de 1993 a 2013. A pesquisa de anticorpos da classe IgG contra T. gondii foi realizada pela Reação de Imunofluorescência Indireta (RIFI); amostras com título ≥ 16 foram consideradas positivas. Entre os cães com sinais clínicos, 259 (43,31%) foram soropositivos para T. gondii com títulos variando de 16 a 4096; já entre os animais hígidos, 79 (22,25%) foram reagentes. Os sinais mais evidentes foram os neurológicos (30,43%) e uveíte (8,03%). Entre as variáveis raça, sexo e idade, apenas a última exerceu influência sobre os resultados observados, com maior frequência de soropositivos em cães idosos. Palavras-chave: caninos; RIFI; sinais clínicos; sorologia; Toxoplasma gondii; zoonose

Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis

Abstract This study aimed to detect parasites from Leishmania genus, to determine the prevalence of anti-Leishmania spp. antibodies, to identify circulating species of the parasite, and to determine epidemiological variables associated with infection in rats caught in urban area of Londrina, Paraná, Brazil. Animal capture was carried out from May to December 2006, serological and molecular methods were performed. DNA was extracted from total blood, and nested-PCR, targeting SSu rRNA from Leishmania genus, was performed in triplicate. The positive samples were sequenced twice by Sanger method to species determination. In total, 181 rodents were captured, all were identified as Rattus rattus and none showed clinical alterations. Forty-one of the 176 (23.3%) animals were positive for Leishmania by ELISA and 6/181 (3.3%) were positive by IFAT. Nine of 127 tested animals (7.1%) were positive by PCR; seven were identified as L. (L.) amazonensis, one as L. (L.) infantum. Four rats were positive using more than one test. This was the first description of synanthropic rodents naturally infected by L. (L.) amazonensis (in the world) and by L. (L.) infantum (in South Brazil). Regarding L. (L.) amazonensis, this finding provides new evidence of the urbanization of this etiological agent

Socioeconomic vulnerability associated to Toxoplasma gondii exposure in southern Brazil.

Human toxoplasmosis, a protozoonosis caused by Toxoplasma gondii, has been described as a worldwide foodborne disease with important public health impact. Despite infection has reportedly varied due to differences in alimentary, cultural and hygienic habits and geographic region, social vulnerability influence on toxoplasmosis distribution remains to be fully established. Accordingly, the present study has aimed to assess T. gondii seroprevalence and factors associated to social vulnerability for infection in households of Ivaiporã, southern Brazil, with 33.6% population making half minimum wage or less, ranked 1,055th in population (31,816 habitants), 1,406th in per capita income (U$ 211.80 per month) and 1,021st in HDI (0.764) out of 5,570 Brazilian cities. Serum samples and epidemiological questionnaires were obtained from citizen volunteers with official City Secretary of Health assistance in 2015 and 2016. In overall, serosurvey has revealed 526/715 (73.57%) positive samples for anti-T. gondii antibodies by Indirect Fluorescent Antibody Test. Logistic regression has shown a significant increase associated to adults (p = 0.021) and elderly (p = 0.014) people, illiterates (p = 0.025), unemployment (p <0.001) and lack of household water tank (p = 0.039). On the other hand, sex (male or female), living area (urban or rural), yard hygiene, meat ingestion, sand or land contact, owning pets (dog, cat or both) were not significant variables of positivity for anti-T. gondii antibodies in the surveyed population. Although no significant spatial cluster was found, high intensity areas of seropositive individuals were located in the Kernel map where the suburban neighborhoods are located. In conclusion, socioeconomic vulnerability determinants may be associated to Toxoplasma gondii exposure. The increased risk due to illiteracy, adult or elderly age, unemployment and lack of household water tank were confirmed by multivariate analysis and the influence of low family income for seropositivity by the spatial analysis

Correction: Spatial and simultaneous representative seroprevalence of anti-Toxoplasma gondii antibodies in owners and their domiciled dogs in a major city of southern Brazil.

[This corrects the article DOI: 10.1371/journal.pone.0180906.]