Data Content Design of eGovernment Application

Bakalářská práce se zabývá problematikou datového modelování v oblasti eGovernmentu, konkrétně tvorby Data transfer object modelu pro novou službu Žádost o důchod online. Součástí práce je teoretická část zabývající se danou problematikou, analytická část, která popisuje východiska a současný stav podání žádosti o důchod, a praktická část, ve které je navržen samotný Data transfer object model Žádost o důchod online.The bachelor thesis deals with eGovernment application data modeling, namely with creation of Data transfer object model of the Pension applying online service. The bachelor thesis includes a theoretical part that concerns the issue, an analytical part that describes the starting points and the current state of pension application and practical part where Data transfer object model of the Pension applying online service is designed.

iP & OEIP - Cytokinin Micro Application Modulates Root Development with High Spatial Resolution

State-of-the-art technology based on organic electronics can be used as a flow-free delivery method for organic substances with high spatial resolution. Such highly targeted drug micro applications can be used in plant research for the regulation of physiological processes on tissue and cellular levels. Here, for the first time, an organic electronic ion pump (OEIP) is reported that can transport an isoprenoid-type cytokinin, N-6-isopentenyladenine (iP), to intact plants. Cytokinins (CKs) are plant hormones involved in many essential physiological processes, including primary root (PR) and lateral root (LR) development. Using the Arabidopsis thaliana root as a model system, efficient iP delivery is demonstrated with a biological output - cytokinin-related PR and LR growth inhibition. The spatial resolution of iP delivery, defined for the first time for an organic compound, is shown to be less than 1 mm, exclusively affecting the OEIP-targeted LR. Results from the application of the high-resolution OIEP treatment method confirm previously published findings showing that the influence of CKs may vary at different stages of LR development. Thus, OEIP-based technologies offer a novel, electronically controlled method for phytohormone delivery that could contribute to unraveling cytokinin functions during different developmental processes with high specificity

Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution

Auxins and cytokinins are two major families of phytohormones that control most aspects of plant growth, development and plasticity. Their distribution in plants has been described, but the importance of cell- and subcellular-type specific phytohormone homeostasis remains undefined. Herein, we revealed auxin and cytokinin distribution maps showing their different organelle-specific allocations within the Arabidopsis plant cell. To do so, we have developed Fluorescence-Activated multi-Organelle Sorting (FAmOS), an innovative subcellular fractionation technique based on flow cytometric principles. FAmOS allows the simultaneous sorting of four differently labelled organelles based on their individual light scatter and fluorescence parameters while ensuring hormone metabolic stability. Our data showed different subcellular distribution of auxin and cytokinins, revealing the formation of phytohormone gradients that have been suggested by the subcellular localization of auxin and cytokinin transporters, receptors and metabolic enzymes. Both hormones showed enrichment in vacuoles, while cytokinins were also accumulated in the endoplasmic reticulum

New Urea Derivatives Are Effective Anti-senescence Compounds Acting Most Likely via a Cytokinin-Independent Mechanism

Stress-induced senescence is a global agro-economic problem. Cytokinins are considered to be key plant anti-senescence hormones, but despite this practical function their use in agriculture is limited because cytokinins also inhibit root growth and development. We explored new cytokinin analogs by synthesizing a series of 1,2,3-thiadiazol-5-yl urea derivatives. The most potent compound, 1-(2-methoxy-ethyl)-3-1,2,3-thiadiazol-5-yl urea (ASES - Anti-Senescence Substance), strongly inhibited dark-induced senescence in leaves of wheat (Triticum aestivum L.) and Arabidopsis thaliana. The inhibitory effect of ASES on wheat leaf senescence was, to the best of our knowledge, the strongest of any known natural or synthetic compound. In vivo, ASES also improved the salt tolerance of young wheat plants. Interestingly, ASES did not affect root development of wheat and Arabidopsis, and molecular and classical cytokinin bioassays demonstrated that ASES exhibits very low cytokinin activity. A proteomic analysis of the ASES-treated leaves further revealed that the senescence-induced degradation of photosystem II had been very effectively blocked. Taken together, our results including data from cytokinin content analysis demonstrate that ASES delays leaf senescence by mechanism(s) different from those of cytokinins and, more effectively. No such substance has yet been described in the literature, which makes ASES an interesting tool for research of photosynthesis regulation. Its simple synthesis and high efficiency predetermine ASES to become also a potent plant stress protectant in biotechnology and agricultural industries

The Cytokinin Complex Associated With Rhodococcus fascians: Which Compounds Are Critical for Virulence?

Virulent strains of Rhodococcus fascians cause a range of disease symptoms, many of which can be mimicked by application of cytokinin. Both virulent and avirulent strains produce a complex of cytokinins, most of which can be derived from tRNA degradation. To test the three current hypotheses regarding the involvement of cytokinins as virulence determinants, we used PCR to detect specific genes, previously associated with a linear virulence plasmid, including two methyl transferase genes (mt1 and mt2) and fas4 (dimethyl transferase), of multiple strains of R. fascians. We inoculated Pisum sativum (pea) seeds with virulent and avirulent strains of R. fascians, monitored the plants over time and compared these to mock-inoculated controls. We used RT-qPCR to monitor the expression of mt1, mt2, and fas4 in inoculated tissues and LC-MS/MS to obtain a comprehensive picture of the cytokinin complement of inoculated cotyledons, roots and shoots over time. The presence and expression of mt1 and mt2 was associated with those strains of R. fascians classed as virulent, and not those classed as avirulent. Expression of mt1, mt2, and fas4 peaked at 9 days post-inoculation (dpi) in cotyledons and at 15 dpi in shoots and roots developed from seeds inoculated with virulent strain 602. Pea plants inoculated with virulent and avirulent strains of R. fascians both contained cytokinins likely to have been derived from tRNA turnover including the 2-methylthio cytokinins and cis-zeatin-derivatives. Along with the isopentenyladenine-type cytokinins, the levels of these compounds did not correlate with virulence. Only the novel 1- and 2-methylated isopentenyladenine cytokinins were uniquely associated with infection by the virulent strains and are, therefore, the likely causative factors of the disease symptoms

Analysis and Architecture of Žádost o důchod online Application

Diplomová práce se zabývá softwarovou analýzou a architekturou aplikace v oblasti eGovernmentu - Žádost o důchod online. Součástí práce jsou teoretická východiska popisující základní pojmy dané problematiky, analytická část, ve které jsou definovány východiska, požadavky, současný stav podání žádosti o důchod a dokončené analytické bloky. V praktické části jsou navrženy samotné analytické a architektonické modely popisující jednotlivé oblasti nově vznikající aplikace Žádost o důchod online.The diploma thesis is focused on software analysis and architecture of the eGovernment application – Žádost o důchod online. It includes theoretical background describing the basic concepts of the issue, the analytical part, which defines starting points, requirements, current state of pension application and completed analytical models. Practical part contains analytical and architecture models describing specific areas of the newly emerging application Žádost o důchod online.

The analysis of foreign trade with wood from selected biomes of the Earth

The object of this diploma thesis is evaluation trends of chosen countries between the years 1990 -- 2010 in division of production, import and export. The next part is description of the year 2010, determination of the main producers, importers, exporters and most significant flows of the industrial roundwoood between countries, regions and continents. The following point is taken into consider other factors like a forest area and population of individual country. These factors strongly influence production, import and export of each country. There were also mentioned the significant benefits and problems of Czech Republic, because of production, import, export and geographical position. Each of the result was presented by graphs and thematic maps. The diploma thesis is based on the statistical information from databases of Food and Agriculture Organization of the United Nations (FAO). These information were evaluated by FAO and estimated like official data about selected countries

Analysis and Architecture of Žádost o důchod online Application

The diploma thesis is focused on software analysis and architecture of the eGovernment application – Žádost o důchod online. It includes theoretical background describing the basic concepts of the issue, the analytical part, which defines starting points, requirements, current state of pension application and completed analytical models. Practical part contains analytical and architecture models describing specific areas of the newly emerging application Žádost o důchod online