Expected and unexpected products of reactions of 2-hydrazinylbenzothiazole with 3-nitrobenzenesulfonyl chloride in different solvents

Acknowledgements We thank the EPSRC National Crystallography Service (University of Southampton) for the X-ray data collections. Funding information MVNdS and JLW thank CNPq (Brazil) for financial support.Peer reviewedPublisher PD

weSPOT: a cloud-based approach for personal and social inquiry

Scientific inquiry is at the core of the curricula of schools and universities across Europe. weSPOT is a new European initiative proposing a cloud-based approach for personal and social inquiry. weSPOT aims at enabling students to create their mashups out of cloud-based tools in order to perform scientific investigations. Students will also be able to share their inquiry accomplishments in social networks and receive feedback from the learning environment and their peers

Applications of Rat Grimace Scale Method in Postoperative Pain Management in Rats

Introduction: Nowadays, 3R (replacement, reduction, refinement) concept lies in the core of laboratory animals science, one of the most important refinement strategies being the pain control. Rat Grimace Scale (RGS) is an actual and effective method used to assess pain in laboratory animals.Aims: The purpose of this study was to find the most effective post surgery analgesic protocol, by grading the changes in the animal’s facial expression.Materials and methods: In the present study we used 5 Wistar rats, as a pain model, ovariectomy was performed on 4 of the rats. Tramadol was administrated subcutaneously as it follows: 1 rat in pre-surgery dose of 25 mg/kg, 2 rats in post-surgery a dose of 25 mg/kg and respectively 50 mg/kg. The evaluation of pain was done across at the time 0, 2, 4 and 6 hours post-surgery using the scores of 0, 1 and 2, 0 representing the absence of pain, 1 a moderate level of pain and 2 a high level of pain.Results: The results of the study revealed that the spayed rat without Tramadol presented the highest level of pain (1.25 - 1.75). The pre-surgery analgesia enhanced the narcosis effect, but analgesia had a short term effect, with a moderate to high level of pain (1 - 1.5). To the animal treated with a dose of 25 mg/kg post-surgery, a moderate pain level was observed (0.5 - 1.25) and to the animal with the highest dose an optimal analgesia was determined (0.25).Conclusion: The expected analgesic effect was put forward by the 50 mg/kg dose of Tramadol and the RGS method was found to be suitable for the assessment of animal suffering in surgical experimental procedures

Пути повышения производительности труда в организации (на примере Брагинского районного потребительского общества)

The purpose of this study was to provide a better understanding why consumers conduct research online and post online reviews. This was achieved by finding relationships between the two phenomena presented above. The study used a cross-sectional design since little was known about the relationships within the chosen research area. Results were measured by quantitative instrument through a self-administered questionnaire divided by each phenomenon. The questionnaire was directed towards Swedish consumers in the buying decision process.Consumers conduct research online with the specific goal for gaining information posted by other consumers, since it has a higher credibility. Helping other consumers and self-expression was reasons found in relation to why consumers post online reviews.Findings strengthen the fact that higher education could be found of relationship to conduct research online; however, not to post online reviews. In comparison to previous studies the study measured online shopping frequency instead of Internet experience. It was found that consumers who conducted online shopping were in relation with previous presented theories.Syftet med denna studie var att skapa en bättre förståelse till varför konsumenter publicerar och konsumerar onlinerecensioner. Genom att applicera en tvärsnittsdesign kunde syftet uppnås då relationer identifierades. För att möjliggöra mätning av resultatet applicerades ett kvantitativt instrument genom en självadministrerad enkät vilken var uppdelad efter respektive fenomen.Onlinerecensioner används för det specifika målet att samla större mängder information innan köpbeslut. De onlinerecensioner som används främst är de skapade av andra konsumenter då de anses vara av högre trovärdighet än information skapat av företag. Att hjälpa andra konsumenter och självförbättring är vad resultatet påvisar vara anledning till att konsumenter publicerar onlinerecensioner.Vidare stärkte resultatet en relation där konsumenter med högre utbildning tenderar att konsumera onlinerecensioner men inte till att publicera dessa. Konsumenternas köpfrekvens applicerades istället för att mäta graden erfarenhet av Internet, vilket gjorts i tidigare studier

THE ACUTE EFFECTS OF A GRIP-CONSTRAINT TOOL ON UPPER BODY AND RACKET KINEMATICS DURING TENNIS FOREHANDS

The purpose of this study was to compare the acute effect of a grip-constraint tool on upper body and racket kinematics during tennis single-handed forehand strokes. Upper-body and racket kinematics for two grip conditions, Preferred (self-selected) and Grip-constraint tool (fixed semi-western forehand grip) were captured for eleven tennis players using a 22-camera Vicon motion capture system (240 Hz). Using a grip-constraint tool resulted in a more closed racket face tilt (~4°) at ball impact while having variations in joint rotations across the shoulder, elbow and wrist. This possibly demonstrates the participant’s ability to self-organise compensatory angular rotations across the upper limb to achieve similar impact orientations. Collectively, these data demonstrate the acute responses to modifying grip technique using a grip-constraint tool during single-handed down-the-line forehands

Retrospective Assessment of Animals Experimentation Projects in Romania – A Critical Analysis of Non-Technical Summaries

The aim of the present inquiry was to design an overview about the non-technical summaries available on the web page of the National Sanitary Veterinary and Food Safety Agency (Romania). We analyzed the compliance to the requirement of replacement, reduction and refinement (the 3R’s). 56 projects were found, the most of them (48), were assigned as “basic research”, 2 as “translational and applied research”, 2 as “regulatory use and routine analysis”, while 4 projects targeted “higher education or training”. The most common animals species used were rats, and mice, followed by rabbits, pigs, hamsters, guinea pigs and frogs (Rana sp.). According to cumulative severity of the project, 20 projects were classified as “severe”, six as “moderate”, three “mild”, and three as “non-recovery”. 24 projects had nor a severity assessment, neither a proper description of the level of severity. In our opinion, the number of projects classified as “severe” is too high; at least for some of them, the level of severity could be reduced using appropriate pain control techniques and / or human end-points. Overall, our recommendation is further improving the knowledge and skills of the personal involved in authorization and execution of the projects and of the authority inspectors that authorized the projects

A correlative analysis of gold nanoparticles internalized by A549 cells

Fluorescently labeled nanoparticles are widely used to investigate nanoparticle cell interactions by fluorescence microscopy. Owing to limited lateral and axial resolution, nanostructures (<100 nm) cannot be resolved by conventional light micro­scopy techniques. Especially after uptake into cells, a common fate of the fluorescence label and the particle core cannot be taken for granted. In this study, a correlative approach is presented to image fluorescently labeled gold nanoparticles inside whole cells by correlative light and electron microscopy (CLEM). This approach allows for detection of the fluorescently labeled particle shell as well as for the gold core in one sample. In this setup, A549 cells are exposed to 8 nm Atto 647N-labeled gold nanoparticles (3.3 × 109 particles mL−1, 0.02 μg Au mL−1) for 5 h and are subsequently imaged by confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). Eight fluorescence signals located at different intracellular positions are further analyzed by TEM. Five of the eight fluorescence spots are correlated with isolated or agglomerated gold nanoparticles. Three fluorescence signals could not be related to the presence of gold, indicating a loss of the particle shell