Antiradical activity of phenolic metabolites extracted from grapes of white and red Vitis vinifera L. cultivars

A diet rich in plant foods is strongly recommended for its beneficial effect on human health. In fact, plant secondary metabolites may exert various biological activities on mammalian cells. Among them, phenolics are excellent natural antioxidants able to rescue cell redox unbalance responsible for the onset of different pathologies. For these reasons, the present work was focused on the study of grape extracts obtained from eight different Italian Vitis vinifera cultivars, quite rare in Italian viticulture and not yet completely chemically characterized. For each preparation, total simple phenolic, flavonoidic and anthocyaninic content was measured through spectrophotometrical assays, while detailed biochemical profile was revealed by LC-MS analyses. In order to valorize the products of these varieties and increase our knowledge about their potential healthy role, the antioxidant power of the samples was evaluated by two different in vitro antiradical tests: DPPH and FRAP. Moreover, free radical scavenging properties of eleven grape pure compounds were investigated, with the aim to: a) compare their real antiradical property with the theoretical one; b) identify which one of them possessed the best bioactivity; c) understand how they might singularly contribute to the nutraceutical effect of the whole grapevine phytocomplex

Covidex: an ultrafast and accurate tool for virus subtyping

Covidex is an open-source, alignment-free machine learning subtyping tool for viral species. It is a shiny app that allows a fast and accurate classification in pre-defined clusters for SARS-CoV-2 and FMDV genome sequences. The user can also build its own classification models with the Covidex model generator.Fil: Cacciabue, Marco Polo Domingo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Aguilera, Pablo Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Gismondi, Maria Ines. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentin

Profile and potential bioactivity of the miRNome and metabolome expressed in Malva sylvestris L. leaf and flower

Malva sylvestris L. (common mallow) is a plant species widely used in phytotherapy and ethnobotanical practices since time immemorial. Characterizing the components of this herb might promote a better comprehension of its biological effects on the human body but also favour the identification of the molecular processes that occur in the plant tissues. Thus, in the present contribution, the scientific knowledge about the metabolomic profile of the common mallow was expanded. In particular, the phytocomplex of leaves and flowers from this botanical species and the extraction capacity of different concentrations of ethanol (i.e., 95%, 70%, 50%, and 0%; v/v in ddH2O) for it were investigated by spectrophotometric and chromatographic approaches. In detail, 95% ethanol extracts showed the worst capacity in isolating total phenols and flavonoids, while all the hydroalcoholic samples revealed a specific ability in purifying the anthocyanins. HPLC–DAD system detected and quantified 20 phenolic secondary metabolites, whose concentration in the several extracts depended on their own chemical nature and the percentage of ethanol used in the preparation. In addition, the stability of the purified phytochemicals after resuspension in pure ddH2O was also proved, considering a potential employment of them in biological/medical studies which include in vitro and in vivo experiments on mammalian models. Here, for the first time, the expressed miRNome in M. sylvestris was also defined by Next Generation Sequencing, revealing the presence of 33 microRNAs (miRNAs), 10 typical for leaves and 2 for flowers. Then, both plant and human putative mRNA targets for the detected miRNAs were predicted by bioinformatics analyses, with the aim to clarify the possible role of these small nucleic acids in the common mallow plant tissues and to try to understand if they could exert a potential cross-kingdom regulatory activity on the human health. Surprisingly, our investigations revealed that 19 miRNAs out of 33 were putatively able to modulate, in the plant cells, the expression of various chromosome scaffold proteins. In parallel, we found, in the human transcriptome, a total of 383 mRNAs involved in 5 fundamental mammalian cellular processes (i.e., apoptosis, senescence, cell-cycle, oxidative stress, and invasiveness) that theoretically could be bound and regulated by M. sylvestris miRNAs. The evidence collected in this work would suggest that the beneficial properties of the use of M. sylvestris, documented by the folk medicine, are probably linked to their content of miRNAs and not only to the action of phytochemicals (e.g., anthocyanins). This would open new perspectives about the possibility to develop gene therapies based on miRNAs isolated from medicinal plants, including M. sylvestris

Investigating plant micro‐remains embedded in dental calculus of the Phoenician inhabitants of Motya (Sicily, Italy)

Plant records reveal remarkable evidence about past environments and human cultures. Exploiting dental calculus analysis and using a combined approach of microscopy and gas chromatography mass spectrometry, our research outlines dietary ecology and phytomedicinal practices of the ancient community of Motya (Sicily, eight to sixth century BC), one of the most important Phoenician settlements in the Mediterranean basin. Micro‐remains suggest use or consumption of Triticeae cereals, and animal‐derived sources (e.g., milk and aquatic birds). Markers of grape (or wine), herbs, and rhizomes, endemic of Mediterranean latitudes and the East, provide insight into the subsistence of this colony, in terms of foodstuffs and phytotherapeutic products. The application of resins and wood of Gymnosperms for social and cultural purposes is hypothesized through the identification of Pinaceae secondary metabolites and pollen grains. The information hidden in dental calculus discloses the strong human‐plant interaction in Motya’s Phoenician community, in terms of cultural traditions and land use

Assessing molecular diversity among 87 species of the Quercus L. genus by RAPD markers

Oaks (Quercus sp.) are among the most ecologically and economically important woody Angiosperms of the northern hemisphere. Nowadays, the reduction of Quercus biodiversity is becoming a matter of global concern, and several oak species have been included in the IUCN Red List of Threatened Species. Consequently, characterization and preservation strategies for the oak germplasm are largely promoted. Thus, in this work, the genetic diversity existing among 87 different Quercus species was assessed using the RAPD markers, in order to better typify these specimens, to show the amazing DNA variability of this plant genus, and to confirm or infer new putative molecular correlations. Our data were discussed taking into consideration the phylogenetic and phylogeographic relationships previously proposed by the literature. In general, the obtained results corroborated that the evolutionary pattern of Quercus genus has been extremely intricate and continues to change rapidly, making it difficult to be fully resolved. The evidence collected in the present investigation would confirm the complex evolution of the oaks, due to their high migration capacity, divergence rate, and hybridization propensity. This research, performed on a so large series of species, represents a positive contribution for highlighting the genetic diversity within collections of Quercus germplasm and favouring ex-situ conservation programmes

Case report of a familial triple: a syndrome and review of the literature

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Sustainability in Aquaponics: Industrial Spirulina Waste as a Biofertilizer for Lactuca sativa L. Plants

Aquaponics represents an alternative to traditional soil cultivation. To solve the problem of nutrient depletion that occurs in this biotechnological system, the application of a spirulina-based biofertilizer was assessed. The microalgal waste used in this study came from industrial processing. Four different dilutions of the supernatant portion of this waste were sprayed on lettuce plants cultivated in an aquaponics system installed at the Botanical Gardens of the Tor Vergata University of Rome. The biofertilizer was characterized to evaluate its amount of macro- and micronutrients. The analysis conducted on the plants involved both morpho-biometric aspects and qualitative–quantitative measurements. The experiments showed that the spirulina extract had a positive effect on the growth and nutraceutical content of the lettuce plants; the obtained results highlighted that a dilution of 75% was the best for treatment. The use of the proposed organic and recycled fertilizer could increase the sustainability of crop cultivation and promote the functioning of aquaponics systems

Biostimulants promoting growth of Vicia faba L. seedlings: inulin coated ZnO nanoparticles

In the present contribution, inulin coated ZnO nanoparticles (ZnO@inu NPs) were investigated for their potential application on crop production systems, by appraisal of their biostimulating effects on Vicia faba L (faba bean). Naked and coated ZnO NPs were synthesized according to purposely implemented eco-friendly protocols and characterized with multiple techniques to determine their crystallographic phase, average particle size, and degree of coating. Faba beans were grown in culture medium supplemented with NPs at 50 or 100 mg kg−1, using ZnO NPs alone, inulin alone, a mixture of the two, or ZnO@inu NPs. Seed germination rate and biometric evaluations on seedlings were carried out, together with Zn localization in the plant tissues. cellular and molecular effects were ascertained by analyses of photosynthetic pigments, cytotoxicity, genotoxicity, viability, induction of oxidative stress and tissue damage, antioxidant response, and modulation of gene expression. these combined studies indicated a potential role of ZnO@inu NPs in promoting growth and development of V. faba seedlings, acting at a post-germinative phase, probably by stimulating the stem cell mitosis. Finally, inulin as a coating agent for the ZnO NPs favored the bioavailability and adsorption of the nanomaterials into the plant tissues, without altering their bioactivity but mitigating any adverse side effect. Graphical Abstract: [Figure not available: see fulltext.]

Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot-and-mouth disease virus

RNA interference (RNAi) is a well-conserved mechanism in eukaryotic cells that directs post-transcriptional gene silencing through small RNA molecules. RNAi has been proposed as an alternative approach for rapid and specific control of viruses including foot-and-mouth disease virus (FMDV), the causative agent of a devastating animal disease with high economic impact. The aim of this work was to assess the antiviral activity of different small RNA shuttles targeting the FMDV RNA-dependent RNA polymerase coding sequence (3D). Three target sequences were predicted within 3D considering RNA accessibility as a major criterion. The silencing efficacy of short-hairpin RNAs (shRNAs) and artificial microRNAs (amiRNAs) targeting the selected sequences was confirmed in fluorescent reporter assays. Furthermore, BHK-21 cells transiently expressing shRNAs or amiRNAs proved 70 to >95% inhibition of FMDV growth. Interestingly, dual expression of amiRNAs did not improve FMDV silencing. Lastly, stable cell lines constitutively expressing amiRNAs were established and characterized in terms of antiviral activity against FMDV. As expected, viral replication in these cell lines was delayed. These results show that the target RNA-accessibility-guided approach for RNAi design rendered efficient amiRNAs that constrain FMDV replication. The application of amiRNAs to complement FMDV vaccination in specific epidemiological scenarios shall be explored further