Airborne and Terrestrial Laser Scanning Data for the Assessment of Standing and Lying Deadwood: Current Situation and New Perspectives

LiDAR technology is finding uses in the forest sector, not only for surveys in producing forests but also as a tool to gain a deeper understanding of the importance of the three-dimensional component of forest environments. Developments of platforms and sensors in the last decades have highlighted the capacity of this technology to catch relevant details, even at finer scales. This drives its usage towards more ecological topics and applications for forest management. In recent years, nature protection policies have been focusing on deadwood as a key element for the health of forest ecosystems and wide-scale assessments are necessary for the planning process on a landscape scale. Initial studies showed promising results in the identification of bigger deadwood components (e.g., snags, logs, stumps), employing data not specifically collected for the purpose. Nevertheless, many efforts should still be made to transfer the available methodologies to an operational level. Newly available platforms (e.g., Mobile Laser Scanner) and sensors (e.g., Multispectral Laser Scanner) might provide new opportunities for this field of study in the near future

La rappresentanza esterna e le Delegazioni dell'Unione Europea: prospettive per un nuovo diritto diplomatico.

The Treaty of Lisbon strengthened the European toolset concerning the external representation of the EU, enshrining new principles and redrawing the institutional framework. Art. 18 TEU made official the role of the High Representative as chief of the European foreign policy. As a consequence, Decision 2010/427/EU was adopted by the Council with the aim of establishing the European External Action Service, the administrative apparatus and cornerstone of the new diplomatic architecture. In the same respect, art. 221 TFEU turned the ‘Commission Delegations’ into ‘Delegations of the EU’ as a whole, providing them with further State-like competences. Indeed, the praxis of recognizing them as proper diplomatic missions in third States, endowed the EU Delegations with advanced prerogatives in coordinating Member States representatives, sharing information and protecting European Union’s interests around the world. Such an analysis, together with the evaluation of EU’s performance in international fora, will be useful in order to understand how EU Delegations carry out the task of channeling diplomacy towards EU institutions ahead of more coherent and effective decisions in foreign policy. Conclusions will be aimed at understanding to what extent the EU transformed (and will transform) the institution of Diplomacy and what are the chances for reaching a common European ‘corps diplomatique’

Emerging and Re-emerging Arboviral Diseases as a Global Health Problem

Newly emerging or re-emerging infections continue to pose significant global public health threats. This chapter provides an overview of the combinations of factors that led to the emergence of arthropod-borne viruses as human and veterinary health threats, in order to understand the risk associated and how this can be mitigated. Considering the history of emergence of some arboviruses, these epidemics have occurred globally as a result of climate and socioeconomic changes that have allowed the spread to new geographical areas of viruses previously confined to specific ecological niches such as West Nile and Chikungunya, or viruses considered under control such as Dengue, Japanese encephalitis, and Yellow fever. Moreover, the greatest risk for humans derives from the ability of these viruses to adopt transmission cycles involving highly anthropophilic mosquito species. Finally, many other arboviruses are largely ignored despite their potential to emerge globally. The recent epidemic spread of Zika virus throughout the Americas is the evidence that arboviruses are likely to continually emerge and re-emerge and that improved scientific technologies and knowledge is essential to deal with future vector-borne epidemics. Research priorities should therefore focus on surveillance systems and vector control tools, as well as on the development of antiviral molecules or candidate vaccine

Toxoplasma gondii in women of childbearing age and during pregnancy: seroprevalence study in Central and Southern Italy from 2013 to 2017

Toxoplasmosis is a worldwide health problem. Infection in pregnant women can result in severe fetal morbidity or in subclinical neonatal infection; most subclinical cases develop ocular and neurological sequelae. The purpose of this serological study was to assess the prevalence of Toxoplasma gondii in two populations of women of childbearing age in Siena (Tuscany, Central Italy) and Bari (Apulia, Southern Italy) between 2013 and 2017 and in a group of pregnant women in Bari in 2016-2017. Serum samples were tested for the presence of specific anti-Toxoplasma gondii IgG antibodies by a commercially available ELISA test. The percentage of seropositive subjects in Bari was significantly higher than in Siena (22.4% vs. 12.4%) and an age-related trend was observed. A low prevalence of T. gondii infection (13.8%) was observed among the pregnant women tested. In addition to showing a significant difference between Central and Southern Italy, this study provides updated data on T. gondii seroprevalence in women during childbearing age and pregnancy. The results confirm a trend toward a decrease, especially in younger people and pregnant women

Epidemiology of herpes simplex virus type 1 and 2 in Italy: a seroprevalence study over 15 years

Introduction: Herpes simplex viruses (HSV) are among the most widespread causative agents of human viral infections. HSV-2 is one of the commonest causes of genital disease, while HSV-1 is associated primarily with orolabial ulceration; however, recent changes in HSV epidemiology showed an increase in genital and neonatal herpes particularly caused by HSV-1. The main purpose of this study was to assess the seroprevalence of HSV-1 and HSV-2 in a random population in Siena (central Italy) in 2000, 2005 and 2013-2014 and in Bari (southern Italy) in 2005. Moreover, a preliminary study was conducted to investigate the spread of HSV infection in a population of pregnant women and infants in Bari in 2003, 2004 and 2005. Methods: Human serum samples were tested for the presence of specific anti-HSV-1 and anti-HSV-2 IgG antibodies using a commercially available ELISA test. Results and conclusions: For the primary purpose, seroprevalence rates observed in Siena were compared over the years sampled and with the seroprevalence rate found in Bari. Results of seroprevalence in Siena show a decreased trend for both viruses, especially in adolescents and young adults; moreover, HSV-2 seroprevalence rates found in the two cities suggest geographical differences. For the secondary purpose, prevalence rates among pregnant women were compared with the seroprevalence found in women of the general population. No significant difference in prevalence rates were found among pregnant women, while results indicate both viruses are a source of infection in infants

Inflation vs. Exhaustion of Antiviral CD8+ T-Cell Populations in Persistent Infections: Two Sides of the Same Coin?

Persistent virus infection can drive CD8+ T-cell responses which are markedly divergent in terms of frequency, phenotype, function, and distribution. On the one hand viruses such as Lymphocytic Choriomeningitis Virus (LCMV) Clone 13 can drive T-cell “exhaustion”, associated with upregulation of checkpoint molecules, loss of effector functions, and diminished control of viral replication. On the other, low-level persistence of viruses such as Cytomegalovirus and Adenoviral vaccines can drive memory “inflation,” associated with sustained populations of CD8+ T-cells over time, with maintained effector functions and a distinct phenotype. Underpinning these divergent memory pools are distinct transcriptional patterns—we aimed to compare these to explore the regulation of CD8+ T-cell memory against persistent viruses at the level of molecular networks and address whether dysregulation of specific modules may account for the phenotype observed. By exploring in parallel and also merging existing datasets derived from different investigators we attempted to develop a combined model of inflation vs. exhaustion and investigate the gene expression networks that are shared in these memory pools. In such comparisons, co-ordination of a critical module of genes driven by Tbx21 is markedly different between the two memory types. These exploratory data highlight both the molecular similarities as well as the differences between inflation and exhaustion and we hypothesize that co-ordinated regulation of a key genetic module may underpin the markedly different resultant functions and phenotypes in vivo—an idea which could be tested directly in future experiments

Warped Wigner-Hough Transform for Defect Reflection Enhancement in Ultrasonic Guided Wave Monitoring

To improve the defect detectability of Lamb wave inspection systems, the application of nonlinear signal processing was investigated. The approach is based on a Warped Frequency Transform (WFT) to compensate the dispersive behavior of ultrasonic guided waves, followed by a Wigner-Ville time-frequency analysis and the Hough Transform to further improve localization accuracy. As a result, an automatic detection procedure to locate defect-induced reflections was demonstrated and successfully tested by analyzing numerically simulated Lamb waves propagating in an aluminum plate. The proposed method is suitable for defect detection and can be easily implemented for real-world structural health monitoring applications

Numerical evaluation of the forest protective role against rockfall after a windthrow: the case study of the Mt. Pore (North-Eastern Italy)

Protection forests effectively preserve people and infrastructures against natural hazards such as rockfall, snow avalanches, landslides, debris flows, soil erosion and floods. Consequently, a reduction of this protective effect due to changes in forest structure can drastically increase the degree of risk related to these phenomena. The Vaia winter storm (29-30 October 2018) affected 41000 ha of forest in North-Eastern Italy, with an amount of windthrow timber volume of 8.6\u2022106 m3, compromising the protective function of a large portion of the forests. The aim of this study is to evaluate changes in the protective effect against rockfall of a forest located on the southern slope of the Mt. Pore after the damages of the Vaia winter storm. Three scenarios have been considered: 1) forest before the windstorm (condition at summer 2018), 2) uncleared windthrow (current status after the windstorm), and 3) cleared windthrow (removal of all fallen dead stems). Rockfall numerical simulations have been pursued considering these three scenarios using the 3D rockfall trajectory model Rockyfor3D. The protective effect of the forest in the three scenarios has been evaluated using the model outputs to calculate quantitative indicators at the distance of the infrastructures at risk. Field surveys and a UAV survey carried out during summer 2018 allowed the collection and the spatialization of the model input parameters, including the forest characteristics before the wind storm. Furthermore, after event on-site investigations allowed the estimation of the windthrow damages and the calibration of the model parameters in the second and third scenarios. Modelling results show that the great amount of dead stems lying on the ground after windthrow (second scenario) can provide protection against rockfall comparable to the living forest (first scenario) in the short period. Effectively, even though the forest and the elements on the ground are not able to stop all the boulders, they can significantly decrease the kinetic energy of the boulders, reducing the speed and rebound height, and decreasing the magnitude of the phenomenon. On the other hand, clearing the windthrow (scenario 3) increase the rockfall risk, especially for the infrastructures at higher distances from the rock cliff. Finally, field surveys supported by remote sensing surveys are revealed essential in order to achieve simulations adherent to reality

SARS-CoV-2 epidemiological trend before vaccination era: a seroprevalence study in Apulia, Southern Italy, in 2020

AimThe present study aimed at assessing the prevalence of antibodies against SARS-CoV-2 in the general population in the province of Bari (Apulia region, Southern Italy) during the year 2020.Subject and methodsIn this study, 1325 serum samples collected from January to December 2020 were tested for the presence of IgM and IgG antibodies against whole-virus SARS-CoV-2 antigen by commercial ELISA. Positive samples were further tested by in-house ELISA for the detection of anti-receptor binding domain (RBD) IgM and IgG antibodies and by micro-neutralization (MN) assay for the detection of neutralizing antibody.ResultsOne hundred (7.55%) samples had the presence of at least one antibody class against SARS-CoV-2 by commercial ELISA, of which 88 (6.6%) showed IgG and 19 (1.4%) showed IgM antibodies. The proportion of samples with IgG antibodies increased from 1.9% in January-February to 9.6% in November-December, while no significant increase was observed for IgM. When tested by in-house ELISA and MN assay, 17.0% and 31.6% were found positive to RBD IgG and RBD IgM, respectively, while 12.0% showed neutralizing antibody.ConclusionThe proportion of samples with SARS-CoV-2 IgG antibodies increased during 2020, especially in the second half of the year, consistent with data reported by the routine epidemiological surveillance of SARS-CoV-2 cases. Despite the high number of reported cases, the seroprevalence values are relatively low, and only a small proportion of samples had neutralizing antibodies