La historia de la matemática en el diseño de e-comics y recursos hipermedia

La historieta es uno de los medios de comunicación más extendidos entre todas las edades y es uno de los recursos comunicativos en continua evolución que más público tiene. Si a ello sumamos el fuerte impacto que ha producido las TIC en estos medios, (concepto de hipermedia), desarrollado un nuevo concepto de historietas, las historietas digitales, no es de dudar entonces, que tenemos un nuevo aliado que debemos llevarlo al aula. A esto hay que sumarle la serie de programas libres tale como Pixton, Powtoon, PowerPoint, etc., para diseñar e-comics con aspectos hipermediales como profesionales. Flores (2012) destaca que si somos capaces de crear un mensaje que rompa las expectativas estaremos introduciendo el mensaje matemático sin tener esa lectura amarga que generalmente se asocia a la clase de matemática

Identification and characterization of PhbF: A DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1

<p>Abstract</p> <p>Background</p> <p><it>Herbaspirillum seropedicae </it>SmR1 is a nitrogen fixing endophyte associated with important agricultural crops. It produces polyhydroxybutyrate (PHB) which is stored intracellularly as granules. However, PHB metabolism and regulatory control is not yet well studied in this organism.</p> <p>Results</p> <p>In this work we describe the characterization of the PhbF protein from <it>H. seropedicae </it>SmR1 which was purified and characterized after expression in <it>E. coli</it>. The purified PhbF protein was able to bind to eleven putative promoters of genes involved in PHB metabolism in <it>H. seropedicae </it>SmR1. <it>In silico </it>analyses indicated a probable DNA-binding sequence which was shown to be protected in DNA footprinting assays using purified PhbF. Analyses using <it>lacZ </it>fusions showed that PhbF can act as a repressor protein controlling the expression of PHB metabolism-related genes.</p> <p>Conclusions</p> <p>Our results indicate that <it>H. seropedicae </it>SmR1 PhbF regulates expression of <it>phb</it>-related genes by acting as a transcriptional repressor. The knowledge of the PHB metabolism of this plant-associated bacterium may contribute to the understanding of the plant-colonizing process and the organism's resistance and survival <it>in planta</it>.</p

Isolation and prebiotic activity of inulin-type fructan extracted from Pfaffia glomerata (Spreng) Pedersen roots

AbstractPfaffia glomerata (Amaranthaceae) is popularly known as “Brazilian ginseng.” Previous studies have shown that fructose is the major carbohydrate component present in its roots. Inulin-type fructans, polymers of fructose, are the most widespread and researched prebiotics. Here, we isolated and chemically characterized inulin extracted from P. glomerata roots and investigated its potential prebiotic effect. Fructans were isolated and their structures were determined using colorimetric, chromatography, polarimetry, and spectroscopic analysis. The degree of polymerization (DP) was determined, and an in vitro prebiotic test was performed. The structure of inulin was confirmed by chromatography and spectroscopic analysis and through comparison with existing data. Representatives from the genera Lactobacillus and Bifidobacterium utilized inulin from P. glomerata, because growth was significantly stimulated, while this ability is strain specific. The results indicated that inulin extracted from P. glomerata roots represents a promising new source of inulin-type prebiotics

Identification and characterization of a new true lipase isolated through metagenomic approach

<p>Abstract</p> <p>Background</p> <p>Metagenomics, the application of molecular genomics to consortia of non-cultivated microbes, has the potential to have a substantial impact on the search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, EC 3.1.1.1) and lipases (triacylglycerol lipases, EC 3.1.1.3). In the current work, a novel lipase gene was identified from a fosmid metagenomic library constructed with the "prokaryotic-enriched" DNA from a fat-contaminated soil collected from a wastewater treatment plant.</p> <p>Results</p> <p>In preliminary screening on agar containing 1% tributyrin, 2661 of the approximately 500,000 clones in the metagenomic library showed activity. Of these, 127 showed activity on agar containing 1% tricaprylin, while 32 were shown to be true lipase producers through screening on agar containing 1% triolein. The clone with the largest halo was further characterized. Its lipase gene showed 72% identity to a putative lipase of <it>Yersinia enterocolitica </it>subsp. <it>palearctica </it>Y11. The lipase, named LipC12, belongs to family I.1 of bacterial lipases, has a chaperone-independent folding, does not possess disulfide bridges and is calcium ion dependent. It is stable from pH 6 to 11 and has activity from pH 4.5 to 10, with higher activities at alkaline pH values. LipC12 is stable up to 3.7 M NaCl and from 20 to 50°C, with maximum activity at 30°C over a 1 h incubation. The pure enzyme has specific activities of 1722 U/mg and 1767 U/mg against olive oil and pig fat, respectively. Moreover, it is highly stable in organic solvents at 15% and 30% (v/v).</p> <p>Conclusions</p> <p>The combination of the use of a fat-contaminated soil, enrichment of prokaryotic DNA and a three-step screening strategy led to a high number of lipase-producing clones in the metagenomic library. The most notable properties of the new lipase that was isolated and characterized were a high specific activity against long chain triacylglycerols, activity and stability over a wide range of pH values, good thermal stability and stability in water-miscible organic solvents and at high salt concentrations. These characteristics suggest that this lipase has potential to perform well in biocatalytic processes, such as for hydrolysis and synthesis reactions involving long-chain triacylglycerols and fatty acid esters.</p

Estudo químico do chá de partes aéreas de amor-crescido (Portulaca pilosa L.) / Chemical study of love-grown aerial parts tea (Portulaca pilosa L.)

 Plantas medicinais são consumidas em formas de chás pelo ser humana desde tempos remotos, nas mais diversas civilizações. Um chá de determinada planta medicinal pode ser útil no combate aos males que afligem a população, sendo, muitas vezes, o único recurso terapêutico disponível, principalmente para os integrantes das classes menos favorecidas da população, porém pode apresentar também algum malefício ao seu consumidor. Esse consumo sempre se baseou no conhecimento empírico das pessoas, sendo ainda escassos trabalhos científicos que colaborem com estudos sobre suas propriedades químicas, e, em especial, teores de metais em chás.  Neste trabalho, teores dos elementos minerais Al, Ba, Ca, K, Mg, Mn, Cr, Cu, Fe, Ni, Sr e Zn foram analisados em amostras de chás das partes aéreas da espécie amor-crescido (Portulaca pilosa L.), coletadas em períodos pluviométricos diferentes. As concentrações destes minerais foram determinadas via espectrometria de emissão ótica por plasma acoplado indutivamente (ICP OES). A composição mineral do chá nos dois períodos analisados (chuvoso e seco) apresentaram valores dentro dos limites permitidos para o consumo humano, a partir da ingestão de no máximo uma xícara de chá de 200 mL por dia, levando-se em consideração fatores dietéticos e a biodisponibilidade destes minerais em cada organismo

Investigation on the Anticonvulsant Potential of Luteolin and Micronized Luteolin in Adult Zebrafish (Danio rerio)

Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Therefore, there is a lack of consensus about the use of luteolin for seizure control. Luteolin low bioavailability could be a limiting factor to obtain better results. Attractively, micronization technology has been applied to improve flavonoids bioavailability. Thus, the present study aimed to investigate the effects of luteolin on its raw form and micronized luteolin in a PTZ-induced seizure model in adult zebrafish (Danio rerio). Our results demonstrate that luteolin and micronized luteolin did not block PTZ-induced seizures in adult zebrafish. Also, luteolin and micronized luteolin did not provoke behavioral changes. Finally, our results show that 24 h after seizure occurrence, no changes were detected for p70S6Kb, interleukin 1β, and caspase-3 transcript levels. Altogether, we failed to observe an anticonvulsant potential of luteolin in adult zebrafish, even in its micronized form. However, we recommend new studies to investigate luteolin benefits in epilepsy.Fil: Garbinato, Cristiane. No especifíca;Fil: Alves Lima Rezende, Cássia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Schneider, Sabrina Ester. No especifíca;Fil: Pedroso, Jefferson. No especifíca;Fil: dos Santos, Aline E.. Universidade Federal de Santa Catarina; BrasilFil: Petry, Fernanda. No especifíca;Fil: Aguiar, Gean Pablo S.. No especifíca;Fil: Girardi Müller, Liz. No especifíca;Fil: Lanza, Marcelo. Universidade Federal de Santa Catarina; BrasilFil: Piato, Angelo. Universidade Federal do Rio Grande do Sul; BrasilFil: Vladimir Oliveira, J.. Universidade Federal de Santa Catarina; BrasilFil: Siebel, Anna Maria. No especifíca

Plasmodium vivax vaccine: What is the best way to go?

Malaria is one of the most devastating human infectious diseases caused by Plasmodium spp. parasites. A search for an effective and safe vaccine is the main challenge for its eradication. Plasmodium vivax is the second most prevalent Plasmodium species and the most geographically distributed parasite and has been neglected for decades. This has a massive gap in knowledge and consequently in the development of vaccines. The most significant difficulties in obtaining a vaccine against P. vivax are the high genetic diversity and the extremely complex life cycle. Due to its complexity, studies have evaluated P. vivax antigens from different stages as potential targets for an effective vaccine. Therefore, the main vaccine candidates are grouped into preerythrocytic stage vaccines, blood-stage vaccines, and transmission-blocking vaccines. This review aims to support future investigations by presenting the main findings of vivax malaria vaccines to date. There are only a few P. vivax vaccines in clinical trials, and thus far, the best protective efficacy was a vaccine formulated with synthetic peptide from a circumsporozoite protein and Montanide ISA-51 as an adjuvant with 54.5% efficacy in a phase IIa study. In addition, the majority of P. vivax antigen candidates are polymorphic, induce strain-specific and heterogeneous immunity and provide only partial protection. Nevertheless, immunization with recombinant proteins and multiantigen vaccines have shown promising results and have emerged as excellent strategies. However, more studies are necessary to assess the ideal vaccine combination and test it in clinical trials. Developing a safe and effective vaccine against vivax malaria is essential for controlling and eliminating the disease. Therefore, it is necessary to determine what is already known to propose and identify new candidates

Obtenção de biodiesel por transesterificação em dois estágios e sua caracterização por cromatografia gasosa: óleos e gorduras em laboratório de química orgânica

Methanolic transesterification of oils and fats was carried out in a two steps procedure, under basic and acidic catalysis. Palm, soybean, canola, corn, rice, grapeseed, sunflower, peanut, pequi and olive oils, besides tallow and lard were used as feedstock. Specific gravity, relative viscosity, thin layer chromatography and gas chromatography were used to characterize the biodiesel. Biodiesel was obtained in high yield and purity. Results were used to discuss the following key-concepts: 1 - triglycerides, composition and properties; 2 - nucleophilic acyl substitution under basic and acid conditions, 3 - thin layer chromatography, 4 - gas chromatography and its quantitative methods

Universidad comprometida : Rescatando PYMES durante la crisis de Covid-19

Efeitos econômicos das políticas de isolamento da pandemia da Covid-19 levaram pequenas e médias empresas (PMEs) a buscar alternativas para sobreviver. Em cenários de crise, uma universidade engajada tem um papel importante ao abordar não apenas questões de saúde, mas também problemas sociais e econômicos em contextos regionais. Neste artigo, um projeto de universidade-comunidade é analisado com o intuito de apontar os elementos necessários para promover uma universidade regional engajada: a Rede de Assessoria SOS-PME, originalmente projetada para apoiar PMEs durante a crise. Uma universidade engajada entrega conhecimento para a sociedade, além de educação e pesquisa. Como resultado, identificam-se elementos para promover a terceira missão da universidade engajada – engajamento social: equipe engajada, multidisciplinaridade, gerenciamento de projetos, agilidade, alianças, estratégia de comunicação, e suporte e reputação da instituiçãoThe economic effects of isolation policies resulting from the COVID-19 pandemic have led small and medium-sized enterprises (SMEs) to look for alternatives to survive. Within this crisis scenario, an engaged university has an important role to play in a regional context in addressing not only health issues, but also any resultant social and economic problems. An engaged university needs to take actions that go beyond its traditional missions of education and research - it has to deliver knowledge to society. This paper analyzes a university-community project in Brazil to identify the necessary elements that help promote a regionally- -engaged university: the SOS-PME Advisory Network project, which was originally designed to assist SMEs during the crisis. As a result, we identified elements necessary for promoting the university’s third mission - social engagement by way of a university-community project: an engaged team, multidisciplinarity, project management, agility, alliances, a communication strategy, institutional support, and reputation.Los efectos económicos de las políticas de aislamiento de la pandemia de COVID-19 han llevado a las pequeñas y medianas empresas (PYMEs) a buscar alternativas para sobrevivir. En un escenario de crisis, una universidad comprometida desempeña un papel importante para abordar, no solo los problemas de salud, sino también los problemas sociales y económicos en un contexto regional. En este artículo, se analiza un proyecto universidad-comunidad con la intención de señalar los elementos necesarios para promover una universidad regional comprometida: la Red de Asesoramiento SOS-PME, originalmente diseñado para apoyar a las PYMEs durante la crisis. Una universidad comprometida necesita llevar adelante acciones más allá de sus misiones tradicionales de educación e investigación; debe transferir conocimiento a la sociedad. Como resultado, identificamos cuáles son los elementos necesarios para promover la tercera misión de una universidad con compromiso social: equipo comprometido, gestión de proyectos, agilidad, alianzas, multidisciplinariedad, estrategia de comunicación y apoyo, y reputación de la institución