Environmental management information system for Thailand's industrial estates.

Our project team designed an Environmental Management Information System prototype for the Industrial Estate Authority of Thailand. By providing effective data management, this tool is a first step toward sustainable development. Using our data analysis methods, and following our recommendations for implementation, the Industrial Estate Authority will be able to build a system that improves decision making throughout Thailand's Industrial Estates. The long-term benefits of this project include reduced waste, increased recycling, and overall environmental and economic improvement

Computational molecular phenotyping of retinal sheet transplants to rats with retinal degeneration

Retinal progenitor sheet transplants have been shown to extend neuronal processes into a degenerating host retina and to restore visual responses in the brain. The aim of this study was the first attempt to identify cells involved in transplant signals to retinal degenerate hosts using computational molecular phenotyping (CMP). - S334ter line 3 rats received fetal retinal sheet transplants at the age of 24-40d. Donor tissues were incubated with slow-releasing microspheres containing BDNF or GDNF. Up to 265 days after surgery, eyes of selected rats were vibratome sectioned through the transplant area (some slices stained for donor marker hPAP), dehydrated and embedded in Eponate, sectioned into serial ultrathin datasets and probed for rhodopsin, cone opsin, CRALBP, L-glutamate, L-glutamine, glutathione, glycine, taurine, GABA, and DAPI. - In large transplant areas, photoreceptor outer segments in contact with host RPE revealed rod and cone opsin immunoreactivity whereas no such staining was found in the degenerate host retina. Transplant photoreceptor layers contained high taurine levels. Glutamate levels in the transplants were higher than in the host retina whereas GABA levels were similar. The transplant inner nuclear layer showed some loss of neurons, but amacrine cells and horizontal cells were not reduced. In many areas, glial hypertrophy between the host and transplant was absent and host and transplant neuropil appeared to intermingle. CMP data indicate that horizontal cells and both glycinergic and GABAergic amacrine cells are involved in a novel circuit between transplant and host, generating alternative signal pathways between transplant and degenerating host retina