Histopathology of myocardial tissue after CLP.

<p>Myocytolysis and contraction bands become evident in the SSI group (upper right panel) compared to the SSI+ALLN group (bottom right panel) 24 h after surgery. The SHAM (upper left panel) and SHAM+ALLN (bottom left panel) groups revealed no morphological alterations. Scale bars indicate 50 mm.</p

Activation of Both the Calpain and Ubiquitin-Proteasome Systems Contributes to Septic Cardiomyopathy through Dystrophin Loss/Disruption and mTOR Inhibition - Fig 5

<p>(<b>A</b>) Western blot analysis of cardiac mTOR protein levels after CLP. Cardiac mTOR protein levels in the SHAM, SSI, SHAM+ALLN and SSI+ALLN groups were measured 24 h after surgery and were expressed in arbitrary units (AUs). GAPDH was used to determine equivalent loading conditions. (<b>B</b>) Analysis of cardiac dystrophin mRNA expression. Dystrophin mRNA expression was measured 24 h after the CLP procedure and expressed in arbitrary units (AUs). GAPDH gene expression was used as the internal control for gene expression normalization. The results (n = 6 per group) are representative of three different experiments.</p

Western blot analysis of cardiac calpain-1, ubiquitin and Pa28β after CLP.

<p>Protein levels of calpain-1 (<b>A</b>), ubiquitin (<b>B</b>) and Pa28β <b>(C)</b> in the SHAM, SSI, SHAM+ALLN and SSI+ALLN groups were measured 24 h after the CLP procedure and expressed in arbitrary units (AUs). GAPDH was used to determine equivalent loading conditions. (<b>D</b>) Calpain-1 mRNA expression was measured 24 h after the CLP procedure and expressed in arbitrary units (AUs). GAPDH gene expression was used as the internal control for gene expression normalization. The results (n = 6 per group) are representative of three different experiments.</p