Control of Fungal Diseases in Mushroom Crops while Dealing with Fungicide Resistance: A Review

Mycoparasites cause heavy losses in commercial mushroom farms worldwide. The negative impact of fungal diseases such as dry bubble (Lecanicillium fungicola), cobweb (Cladobotryum spp.), wet bubble (Mycogone perniciosa), and green mold (Trichoderma spp.) constrains yield and harvest quality while reducing the cropping surface or damaging basidiomes. Currently, in order to fight fungal diseases, preventive measurements consist of applying intensive cleaning during cropping and by the end of the crop cycle, together with the application of selective active substances with proved fungicidal action. Notwithstanding the foregoing, the redundant application of the same fungicides has been conducted to the occurrence of resistant strains, hence, reviewing reported evidence of resistance occurrence and introducing unconventional treatments is worthy to pave the way towards the design of integrated disease management (IDM) programs. This work reviews aspects concerning chemical control, reduced sensitivity to fungicides, and additional control methods, including genomic resources for data mining, to cope with mycoparasites in the mushroom industry

Screening and Evaluation of Essential Oils from Mediterranean Aromatic Plants against the Mushroom Cobweb Disease, Cladobotryum mycophilum

The main aim of this study was to evaluate the use of essential oils (EOs) as an alternative to synthetic fungicides used in the control of cobweb disease of button mushroom (Agaricus bisporus) caused by Cladobotryum mycophilum. The EOs used were obtained by hydrodistillation from five Mediterranean aromatic species (Lavandula × intermedia, Salvia lavandulifolia, Satureja montana, Thymus mastichina, and Thymus vulgaris), analyzed by gas chromatography, and tested in vitro for their antifungal activity against C. mycophilum. In vitro bioassays showed that the EOs obtained from T. vulgaris and S. montana (ED50 = 35.5 and 42.8 mg L−1, respectively) were the most effective EOs for inhibiting the mycelial growth of C. mycophilum, and were also the most selective EOs between C. mycophilum and A. bisporus. The in vivo efficacy of T. vulgaris and S. montana EOs at two different concentrations (0.5 and 1%) were evaluated in two mushroom growing trials with C. mycophilum inoculation. The treatments involving T. vulgaris and S. montana EOs at the higher dose (1% concentration) were as effective as fungicide treatment. The effect of these EOs on mushroom productivity was tested in a mushroom cropping trial without inoculation. They had a strong fungitoxic effect at the first flush. However, a compensatory effect was observed by the end of the crop cycle and no differences were observed in biological efficiency between treatments. The main compounds found were carvacrol and p-cymene for S. montana, and p-cymene and thymol for T. vulgaris. These results suggest that T. vulgaris and S. montana EOs may be useful products to manage cobweb disease if used as part of an integrated pest management (IPM) program

Genetic Analysis and In Vitro Enzymatic Determination of Bacterial Community in Compost Teas from Different Sources

16 páginas.-- 2 figuras.-- 5 tablas.-- 53 referenciasCompost tea has been used throughout the world to control diseases and promote the growth of plants. The microbial community is the main factor involved both in the antagonistic effect against relevant phytopathogens and as a stimulator of plant growth. The objective of this research is to determine the bacterial diversity present in four types of compost as well as some of the mechanisms that may be involved in the positive effects of compost tea on crops. Aerated (ACT) and non-aerated (NCT) compost teas were obtained from four different composts: spent mushroom substrate compost (SMC), grape marc compost (GMC), greenhouse horticultural crop residues compost (CRC), and vermicompost (CRV). 16S rDNA-based DGGE profiles were obtained for each compost tea and their respective bacterial communities were analyzed. Of the 100 clones obtained, those typical for being dominant for all profiles were chosen. In total, 20 different clones were sequenced. The results showed that the bacterial communities of most compost teas had high richness, diversity, and evenness values, with relative abundance of species belonging to Bacteroidetes and Proteobacteria. The NCTs obtained from all the composts, especially CRC and CRV, showed high levels of siderophore production, while teas from GMC manifested high and consistent cellulase activity. The ACTs from all the composts, especially SMC, had high protease activityThe present work benefited from the input of the project RTA2010-00011-C02-02. “Caracterización microbiológica y optimización de los mecanismos de supresividad de sustratos postcultivos de hongos comestibles frente a enfermedades del champiñón, ” from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) supported by the Spanish Ministry of Science and Innovation.Peer reviewe

Mercado actual para los profesionales del periodismo en la Región Metropolitana y su tendencia a 1998

In this work, a mechanochemical procedure is proposed as a simple and fast method to synthesize the pure BiFeO3 perovskite phase as a nanostructured material without the need for purification treatments, while the mechanochemical reaction mechanism has been investigated and correlated with that of the conventional solid-state reaction. Thus, different milling conditions have been used as a tool for tailoring the crystallite size of the resulting BiFeO3 nanoparticles. The materials prepared by the mechanochemical reaction could be annealed or sintered without the formation of undesirable phases. Both the ferroelectric and ferromagnetic transitions were observed by DSC. Finally, the dielectric constants of the prepared material at different frequencies as a function of the temperature have been measured, showing that the material is clearly an isolator below 200 °C, characteristic of a high quality BiFeO3 material.Gobierno de España CTQ 2011-27626Junta de Andalucía TEP-03002, TEP-785