MUC1 positive cutaneous metastasis with transepidermal elimination from a breast carcinoma

Breast cancer is the most common cause of cutaneous metastases from internal malignancies. Generally, the neoplastic cells are located in the dermis or hypodermis, while a finding of transepidermal elimination on cutaneous metastases is exceptional. In this report we present a patient with perforating cutaneous metastases from breast cancer with mucin 1 expression. Cutaneous, bone, lung, and hepatic lesions were detected two years after the diagnosis of the primary tumor.Fil: Luna, Amalia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Merino, María Emilia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Alberdi, Cecilio G.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segal Eiras, Amada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Croce, María Virginia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Natural mortality of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) eggs in Argentina and Italy, and first record of Encarsia porteri (Mercet) (Hymenoptera: Aphelinidae) affecting its populations

En este estudio se evaluaron los agentes oófagos de T. absoluta de presencia espontánea en cultivos de tomate en Sudamérica (NOA y Cinturón Hortícola Platense, Argentina) y en Europa (Lazio, Italia). Se reporta el listado de especies encontradas y las tasas de mortalidad natural por parasitismo y depredación en cultivos de ambos continentes. Además, se informa el primer registro de Encarsia porteri (Mercet) (Hymenoptera: Aphelinidae) parasitando huevos de la polilla.Oophagous biological control agents of T. absoluta naturally present in crops in South America (Buenos Aires and Tucumán Provinces, Argentina) and Europe (Lazio, Italia) were surveyed. A taxonomic list of species and the natural mortality rates caused by parasitism and predation are reported. Furthermore, the first record of Encarsia porteri (Mercet) (Hymenoptera: Aphelinidae) parasitizing T. absoluta eggs is informed.Fil: Luft, Érica. San Miguel de Tucumán (Tucumán, Argentina). CONICET - Planta Piloto de Procesos Industriales Microbiológicos-Biotecnología, Div. Control Biológico.Fil: Luna, María Gabriela. Universidad Nacional de La PlataFil: Galise, Gabriele. Italia. Università degli Studi della Tuscia.Fil: Speranza, Stefano. Italia. Università degli Studi della Tuscia.Fil: Virla, Eduardo G.. San Miguel de Tucumán (Tucumán, Argentina). CONICET - Planta Piloto de Procesos Industriales Microbiológicos-Biotecnología, Div. Control Biológico

Desarrollo y morfología del fruto y la semilla del género hemiparásito <i>Jodina</i> (Cervantesiaceae)

The monotypic genus Jodina is endemic to central and southeastern South America, common in forests and scrubland environments. The fruit type in Jodina is controversial since it has been described at different stages of development. The main objective of this work was to analyze the morphology of the fruit of J. rhombifolia during its maturation to attain a consensus about its type. Also characteristics of seed development and anatomy were analyzed in detail. Material was processed according to conventional techniques for LM and SEM studies. The fruit of J. rhombifolia is a pseudodrupe since the ovary is half-inferior; the fleshy layer of the pericarp is constituted by an increase of the nectary disk whereas the stony layer is represented by the mesocarp. Participation of the nectary disk as part of the fruit wall has not been mentioned previously. The seed is "naked" -the integument disintegrates during development- and the resulting structure is a pyrene. The interpretations made by other authors on the fruit of Jodina are also discussed.El género monotípico Jodina es endémico del centro y sureste de América central, común en bosques y ambientes de matorral. El tipo de fruto en Jodina es controvertido, ya que se ha descrito en diferentes etapas de su desarrollo. El principal objetivo de este trabajo fue analizar la morfología del fruto de J. rhombifolia durante su maduración para alcanzar un consenso sobre su tipo. Asimismo, se analizaron en detalle diferentes características del desarrollo y la anatomía de la semilla. El material se estudió de acuerdo con las técnicas convencionales utilizadas en estudios con MO y MEB. El fruto de J. rhombifolia es una pseudodrupa, dado que el ovario es semiínfero; la capa carnosa del pericarpio está constituida por un engrosamiento del disco y la capa dura está representada por el mesocarpio. La participación del disco como parte de la pared del fruto no se ha mencionado anteriormente. La semilla es “desnuda” —el tegumento se desintegra durante el desarrollo— y la estructura resultante es un pireno. Se discuten asimismo las interpretaciones realizadas por otros autores acerca del fruto de Jodina.Facultad de Ciencias Naturales y Muse

Desarrollo y morfología del fruto y la semilla del género hemiparásito <i>Jodina</i> (Cervantesiaceae)

The monotypic genus Jodina is endemic to central and southeastern South America, common in forests and scrubland environments. The fruit type in Jodina is controversial since it has been described at different stages of development. The main objective of this work was to analyze the morphology of the fruit of J. rhombifolia during its maturation to attain a consensus about its type. Also characteristics of seed development and anatomy were analyzed in detail. Material was processed according to conventional techniques for LM and SEM studies. The fruit of J. rhombifolia is a pseudodrupe since the ovary is half-inferior; the fleshy layer of the pericarp is constituted by an increase of the nectary disk whereas the stony layer is represented by the mesocarp. Participation of the nectary disk as part of the fruit wall has not been mentioned previously. The seed is "naked" -the integument disintegrates during development- and the resulting structure is a pyrene. The interpretations made by other authors on the fruit of Jodina are also discussed.El género monotípico Jodina es endémico del centro y sureste de América central, común en bosques y ambientes de matorral. El tipo de fruto en Jodina es controvertido, ya que se ha descrito en diferentes etapas de su desarrollo. El principal objetivo de este trabajo fue analizar la morfología del fruto de J. rhombifolia durante su maduración para alcanzar un consenso sobre su tipo. Asimismo, se analizaron en detalle diferentes características del desarrollo y la anatomía de la semilla. El material se estudió de acuerdo con las técnicas convencionales utilizadas en estudios con MO y MEB. El fruto de J. rhombifolia es una pseudodrupa, dado que el ovario es semiínfero; la capa carnosa del pericarpio está constituida por un engrosamiento del disco y la capa dura está representada por el mesocarpio. La participación del disco como parte de la pared del fruto no se ha mencionado anteriormente. La semilla es “desnuda” —el tegumento se desintegra durante el desarrollo— y la estructura resultante es un pireno. Se discuten asimismo las interpretaciones realizadas por otros autores acerca del fruto de Jodina.Facultad de Ciencias Naturales y Muse

MUC1 positive cutaneous metastasis with transepidermal elimination from a breast carcinoma

Breast cancer is the most common cause of cutaneous metastases from internal malignancies. Generally, the neoplastic cells are located in the dermis or hypodermis, while a finding of transepidermal elimination on cutaneous metastases is exceptional. In this report we present a patient with perforating cutaneous metastases from breast cancer with mucin 1 expression. Cutaneous, bone, lung, and hepatic lesions were detected two years after the diagnosis of the primary tumor.Centro de Investigaciones Inmunológicas Básicas y Aplicada

Borrelia burgdorferi infection in a natural population of Peromyscus leucopus mice: A longitudinal study in an area where lyme borreliosis is highly endemic

Blood samples from Peromyscus leucopus mice captured at an enzootic site in Connecticut were examined for antibodies to and DNA of Borrelia burgdorferi, to characterize the dynamics of infection in this reservoir population. From trappings conducted over the course of 2 transmission seasons, 598 (75%) of 801 serum samples from 514 mice were found to be positive by enzyme immunoassay. Seropositivity correlated with date of capture and mouse age, was similar among locations within the site, increased from 57% to 93% over the course of the transmission season, and was associated with antibodies to outer surface protein (Osp) C, but not to OspA. Longitudinal samples from 184 mice revealed an incidence of 0.2 cases/mouse/week. Nineteen (10%) of 187 samples were found by polymerase chain reaction to be positive for B. burgdorferi, and, of those, 14 (74%) were found to be seropositive. Nearly the entire population of P. leucopus mice became infected with B. burgdorferi by late August, coinciding with the peak activity period of host-seeking larvae uninfected with the spirochete Ixodes scapularis, thereby perpetuating the agent through succeeding generations of ticks.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Naturales y Muse

Borrelia burgdorferi infection in a natural population of Peromyscus leucopus mice: A longitudinal study in an area where lyme borreliosis is highly endemic

Blood samples from Peromyscus leucopus mice captured at an enzootic site in Connecticut were examined for antibodies to and DNA of Borrelia burgdorferi, to characterize the dynamics of infection in this reservoir population. From trappings conducted over the course of 2 transmission seasons, 598 (75%) of 801 serum samples from 514 mice were found to be positive by enzyme immunoassay. Seropositivity correlated with date of capture and mouse age, was similar among locations within the site, increased from 57% to 93% over the course of the transmission season, and was associated with antibodies to outer surface protein (Osp) C, but not to OspA. Longitudinal samples from 184 mice revealed an incidence of 0.2 cases/mouse/week. Nineteen (10%) of 187 samples were found by polymerase chain reaction to be positive for B. burgdorferi, and, of those, 14 (74%) were found to be seropositive. Nearly the entire population of P. leucopus mice became infected with B. burgdorferi by late August, coinciding with the peak activity period of host-seeking larvae uninfected with the spirochete Ixodes scapularis, thereby perpetuating the agent through succeeding generations of ticks.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Naturales y Muse

Anatomical and chemical modifications in the wood of specimens of trees of La Plata city (Buenos Aires) caused by Laetiporus sulphureus (Basidiomycota, Polyporales).

A nivel mundial, Laetiporus sulphureus es un importante patógeno causante de pudriciones castañas en el arbolado urbano. En la ciudad de La Plata, dicho hongo fue hallado en ejemplares de álamo blanco, fresno americano, eucalipto y paraíso gigante. El objetivo del trabajo fue analizar las alteraciones estructurales y químicas ocasionadas por L. sulphureus en el leño de álamo blanco, fresno americano, eucalipto y paraíso gigante con el propósito de dimensionar el riesgo que implica su deterioro para los habitantes, sus bienes y la diversidad arbórea de la ciudad. Los estudios anatómicos fueron realizados con microscopios óptico y electrónico de barrido. Los análisis químicos se llevaron a cabo en muestras de fresno y paraíso, según normas TAPPI. En todos los ejemplares el daño se localizó en el duramen, registrándose caracteres típicos de estadios avanzados de pudrición castaña. En cada especie se registraron alteraciones particulares a nivel anatómico y químico, compatibles con las diferencias estructurales entre ellas. Esta es la primera contribución regional sobre el tipo y grado de deterioro registrados en especies infectadas naturalmente por L. sulphureus, de utilidad para la comprensión de los riesgos que ello implica en el contexto urbano.L. sulphureus is an important pathogen causing brown rot in urban trees worldwide. In La Plata city, this fungus was found in specimens of white poplar, green ash, eucalyptus and chinaberry. The objective of this study was to analyze the structural and chemical alterations caused by L. sulphureus in white poplar, green ash, eucalyptus and chinaberry in order to contribute to the diagnosis of the risk that implies their deterioration for inhabitants, their goods and tree diversity in the city. Anatomical studies were performed using light and scanning electron microscopes. Chemical analyses were carried out on samples of green ash and chinaberry, according to TAPPI standards. In all instances the damage was located in the heartwood, showing typical characteristics of advanced stages of brown rot decay. For each species specific alterations at the anatomical and chemical levels were registered, compatible with their structural differences. This is the first regional contribution on the type and degree of deterioration recorded in species naturally affected by L. sulphureus, useful for understanding the risks involved in the urban context.Facultad de Ciencias Agrarias y Forestale

Anatomical and chemical modifications in the wood of specimens of trees of La Plata city (Buenos Aires) caused by Laetiporus sulphureus (Basidiomycota, Polyporales).

A nivel mundial, Laetiporus sulphureus es un importante patógeno causante de pudriciones castañas en el arbolado urbano. En la ciudad de La Plata, dicho hongo fue hallado en ejemplares de álamo blanco, fresno americano, eucalipto y paraíso gigante. El objetivo del trabajo fue analizar las alteraciones estructurales y químicas ocasionadas por L. sulphureus en el leño de álamo blanco, fresno americano, eucalipto y paraíso gigante con el propósito de dimensionar el riesgo que implica su deterioro para los habitantes, sus bienes y la diversidad arbórea de la ciudad. Los estudios anatómicos fueron realizados con microscopios óptico y electrónico de barrido. Los análisis químicos se llevaron a cabo en muestras de fresno y paraíso, según normas TAPPI. En todos los ejemplares el daño se localizó en el duramen, registrándose caracteres típicos de estadios avanzados de pudrición castaña. En cada especie se registraron alteraciones particulares a nivel anatómico y químico, compatibles con las diferencias estructurales entre ellas. Esta es la primera contribución regional sobre el tipo y grado de deterioro registrados en especies infectadas naturalmente por L. sulphureus, de utilidad para la comprensión de los riesgos que ello implica en el contexto urbano.L. sulphureus is an important pathogen causing brown rot in urban trees worldwide. In La Plata city, this fungus was found in specimens of white poplar, green ash, eucalyptus and chinaberry. The objective of this study was to analyze the structural and chemical alterations caused by L. sulphureus in white poplar, green ash, eucalyptus and chinaberry in order to contribute to the diagnosis of the risk that implies their deterioration for inhabitants, their goods and tree diversity in the city. Anatomical studies were performed using light and scanning electron microscopes. Chemical analyses were carried out on samples of green ash and chinaberry, according to TAPPI standards. In all instances the damage was located in the heartwood, showing typical characteristics of advanced stages of brown rot decay. For each species specific alterations at the anatomical and chemical levels were registered, compatible with their structural differences. This is the first regional contribution on the type and degree of deterioration recorded in species naturally affected by L. sulphureus, useful for understanding the risks involved in the urban context.Facultad de Ciencias Agrarias y Forestale

An ecological approach to preventing human infection: Vaccinating wild mouse reservoirs intervenes in the Lyme disease cycle

Many pathogens, such as the agents of West Nile encephalitis and plague, are maintained in nature by animal reservoirs and transmitted to humans by arthropod vectors. Efforts to reduce disease incidence usually rely on vector control or immunization of humans. Lyme disease, for which no human vaccine is currently available, is a commonly reported vector-borne disease in North America and Europe. In a recently developed, ecological approach to disease prevention, we intervened in the natural cycle of the Lyme disease agent (Borrelia burgdorferi) by immunizing wild white-footed mice (Peromyscus leucopus), a reservoir host species, with either a recombinant antigen of the pathogen, outer surface protein A, or a negative control antigen in a repeated field experiment with paired experimental and control grids stratified by site. Outer surface protein A vaccination significantly reduced the prevalence of B. burgdorferi in nymphal blacklegged ticks (Ixodes scapularis) collected at the sites the following year in both experiments. The magnitude of the vaccine's effect at a given site correlated with the tick infection prevalence found on the control grid, which in turn correlated with mouse density. These data, as well as differences in the population structures of B. burgdorferi in sympatric ticks and mice, indicated that nonmouse hosts contributed more to infecting ticks than previously expected. Thus, where nonmouse hosts play a large role in infection dynamics, vaccination should be directed at additional species.Facultad de Ciencias Naturales y MuseoCentro de Estudios Parasitológicos y de Vectore