N′-(4-Hy­droxy­benzyl­idene)ferrocene-1-carbohydrazide

In the title compound, [Fe(C5H5)2(C13H11N2O2)], the dihedral angle between the benzene ring and the cyclo­penta­diene ring bonded to the carbonyl group is 26.1 (2)°. In the crystal, bifurcated O—H⋯(O,N) and N—H⋯O hydrogen bonds link the mol­ecules into a three-dimensional network

The effects of three different grinding methods in DNA extraction of cowpea (Vigna unguiculata L. Walp)

Rapid DNA extraction is a prerequisite for molecular studies. Generally, plant tissue is ground in liquid nitrogen to isolate DNA; but, liquid nitrogen is dangerous and volatile. Besides, liquid nitrogen is not always available in many developing countries. To investigate if high quality DNA could be obtained for downstream PCR analysis without liquid nitrogen, the cowpea DNA was extracted by Hexadecyl trimethyl ammonium bromide cetyl trimethylammonium bromide (CTAB) method and sodium dodecyl sulphate (SDS) method, respectively, each with three different grinding methods, including ground in liquid nitrogen, in preheated mortar and in non-preheated mortar. The DNA was compared according to their yield, purity, integrity and functionality. The results showed that high quality DNA could be obtained by three grinding methods both in CTAB method and SDS method. Without liquid nitrogen, grinding plant tissue in preheated or non-preheated mortar with extraction buffer to extract DNA is feasible.Keywords: Cowpea (Vigna unguiculata), grinding method, liquid nitrogen, DNA extractionAfrican Journal of Biotechnology Vol. 12(16), pp. 1946-195

5-(4-Methyl­phen­yl)-1,3,4-oxadiazol-2-amine

In the crystal structure of the title compound, C9H9N3O, adjacent mol­ecules are linked through N—H⋯N hydrogen bonds into a three-dimensional network

A Hybrid Multi-objective Genetic Algorithm for Bi-objective Time Window Assignment Vehicle Routing Problem

Providing a satisfying delivery service is an important way to maintain the customers’ loyalty and further expand profits for manufacturers and logistics providers. Considering customers’ preferences for time windows, a bi-objective time window assignment vehicle routing problem has been introduced to maximize the total customers’ satisfaction level for assigned time windows and minimize the expected delivery cost. The paper designs a hybrid multi-objective genetic algorithm for the problem that incorporates modified stochastic nearest neighbour and insertion-based local search. Computational results show the positive effect of the hybridization and satisfactory performance of the metaheuristics. Moreover, the impacts of three characteristics are analysed including customer distribution, the number of preferred time windows per customer and customers’ preference type for time windows. Finally, one of its extended problems, the bi-objective time window assignment vehicle routing problem with time-dependent travel times has been primarily studied.</p

Verification of functional and non-functional requirements of web service composition

Abstract. Web services have emerged as an important technology nowadays. There are two kinds of requirements that are crucial to web service composition, which are functional and non-functional requirements. Functional requirements focus on functionality of the composed service, e.g., given a booking service, an example of functional requirements is that a flight ticket with price higher than $2000 will never be purchased. Non-functional requirements are concerned with the quality of service (QoS), e.g., an example of the booking service’s non-functional requirements is that the service will respond to the user within 5 sec-onds. Non-functional requirements are important to web service composition, and are often an important clause in service-level agreements (SLAs). Even though the functional requirements are satisfied, a slow or unreliable service may still not be adopted. In our paper, we propose an automated approach to verify combined functional and non-functional requirements directly based on the semantics of web service composition. Our approach has been implemented and evaluated on the real-world case studies, which demonstrate the effectiveness of our method.

Low dose triptolide reverses chemoresistance in adult acute lymphoblastic leukemia cells via reactive oxygen species generation and DNA damage response disruption

Chemoresistance represents a major challenge for treatment of acute lymphoblastic leukemia (ALL). Thus, new drugs to overcome chemoresistance in ALL are urgently needed. To this end, we established a cytarabine (araC)-resistant ALL cell line (NALM-6/R), which interestingly displayed cross-resistance towards doxorubicin (ADM). Here we report that low dose of triptolide (TPL), a natural product used for treating inflammatory diseases such as arthritis, could reverse araC and ADM resistance and in NALM-6/R cells as well as primary cells from patients with relapsed or refractory (R/R) ALL, reflected by inhibition of cell proliferation and induction of apoptosis in vitro, and repression of tumor growth in vivo in a mouse xenograft model. Mechanistically, these events were associated with impaired mitochondrial membrane potential and increased reactive oxygen species (ROS) production. Co-treatment with TPL and araC or ADM upregulated pro-apoptotic caspase-9 protein, inhibited checkpoint kinase 1 (Chk1) and 2 (Chk2) phosphorylation, and induced γH2A.X (a DNA damage marker). Notably, the combination regimen of TPL and conventional chemotherapeutics also rapidly diminished tumor burden in a patient with R/R ALL. Together, these findings provide preclinical evidence for repurposing use of TPL in combination with chemotherapeutic agents to treat R/R ALL as an alternative salvage regimen

In Vivo Blood Glucose Quantification Using Raman Spectroscopy

We here propose a novel Raman spectroscopy method that permits the noninvasive measurement of blood glucose concentration. To reduce the effects of the strong background signals produced by surrounding tissue and to obtain the fingerprint Raman lines formed by blood analytes, a laser was focused on the blood in vessels in the skin. The Raman spectra were collected transcutaneously. Characteristic peaks of glucose (1125 cm(-1)) and hemoglobin (1549 cm(-1)) were observed. Hemoglobin concentration served as an internal standard, and the ratio of the peaks that appeared at 1125 cm(-1) and 1549 cm(-1) peaks was used to calculate the concentration of blood glucose. We studied three mouse subjects whose blood glucose levels became elevated over a period of 2 hours using a glucose test assay. During the test, 25 Raman spectra were collected transcutaneously and glucose reference values were provided by a blood glucose meter. Results clearly showed the relationship between Raman intensity and concentration. The release curves were approximately linear with a correlation coefficient of 0.91. This noninvasive methodology may be useful for the study of blood glucose in vivo

Scaling BDD-based timed verification with simulation reduction

Digitization is a technique that has been widely used in real-time model checking. With the assumption of digital clocks, symbolic model checking techniques (like those based on BDDs) can be applied for real-time systems. The problem of model checking real-time systems based on digitization is that the number of tick transitions increases rapidly with the increment of clock upper bounds. In this paper, we propose to improve BDD-based verification for real-time systems using simulation reduction. We show that simulation reduction allows us to verify timed automata with large clock upper bounds and to converge faster to the fixpoint. The presented approach is applied to reachability and LTL verification for real-time systems. Finally, we compare our approach with existing tools such as Rabbit, Uppaal, and CTAV and show that our approach outperforms them and achieves a significant speedup.No Full Tex