11 research outputs found
The Protein-Templated Synthesis of Enzyme-Generated Aptamers.
Inspired by the chemical synthesis of molecularly imprinted polymers, we demonstrated for the first time, the protein-target mediated synthesis of enzyme-generated aptamers (EGAs). We prepared pre-polymerisation mixtures containing different ratios of nucleotides, an initiator sequence and protein template and incubated each mixture with terminal deoxynucleotidyl transferase (TdT). Upon purification and rebinding of the EGAs against the target, we observed an enhancement in binding of templated-EGAs against the target compared to a non-templated control. These results demonstrate the presence of two primary mechanisms for the formation of EGAs, namely, the binding of random sequences to the target as observed in systematic evolution of ligands by exponential enrichment (SELEX) and the dynamic competition between TdT enzyme and the target protein for binding of EGAs during synthesis. The latter mechanism serves to increase the stringency of EGA-based screening and represents a new way to develop aptamers that relies on rational design
Chemical Analysis of H. pomatia Mucus Constituents: A study investigating the main constituents of the adhesive mucus extracted from H. pomatia snails, and the variation in the chemical composition of mucus over time and under different conditions
Chemical and Antimicrobial Analysis of Mucus from Helix aspersa and Helix pomatia Snail Species
the Influence of Lysine Acetylation on The Cellular Physiology, Growth, and DNA Replication Initiation of Escherichia coli Cells: A study investigating the effect of deletion of genes (pta, ackA, YfiQ, and cobB) coding for central enzymes involved in the process of lysine acetylation on the cellular physiology, growth, and DNA replication initiation of Escherichia coli cells.
Ethical aspects of alternative treatment methods for certain disorders and their current commercial ones
How is Helicobacter pylori's efficiency at forming stomach ulcers related to a factors - tobacco, alcohol, caffeine?
The Protein-Templated Synthesis of Enzyme-Generated Aptamers
Inspired by the chemical synthesis of molecularly imprinted polymers, we demonstrated for the first time, the protein‐target mediated synthesis of enzyme‐generated aptamers (EGAs). We prepared pre‐polymerisation mixtures containing different ratios of nucleotides, an initiator sequence and protein template and incubated each mixture with terminal deoxynucleotidyl transferase (TdT). Upon purification and rebinding of the EGAs against the target, we observed an enhancement in binding of templated‐EGAs towards the target compared to a non‐templated control. These results demonstrate the presence of two primary mechanisms for the formation of EGAs, namely, the binding of random sequences to the target as observed in systematic evolution of ligands by exponential enrichment (SELEX) and the dynamic competition between TdT enzyme and the target protein for binding of EGAs during synthesis. The latter mechanism serves to increase the stringency of EGA‐based screening and represents a new way to develop aptamers that relies on rational design